Antibiotic Resistance in Escherichia coli and Staphylococcus aureus from Retail Chicken Meat in Surabaya, Indonesia
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Introduction: Antimicrobial resistance is becoming a problem in public health. Zoonotic food-borne bacteria is infectious agent that can be transferred from animal to human through the food-producing animal we consume. Nowadays, antibiotic used for human and animal is not only to cure infection but also to aim animal's growth promotion. It is known as non therapeutic antimicrobial agent (NTA) leading to antibiotic resistance. The third generation cephalosporins, cefotaxime, and also cefoxitin are included as important antibiotic for human. This study aims to identify the presence of cefotaxime-resistant Escherichia coli and cefoxitin-resistant Staphylococcus aureus isolated from chicken meat of both traditional and modern market in Surabaya.
Methods: This is descriptive post test only experimental research. We used 8 samples of chicken meat from 4 different market using purposive sampling technique. We cultured Escherichia coli and Staphylococcus aureus from the chicken meat. Sensitivity test was done using Kirby-bauer disk-diffusion method.
Results: All chicken meat sample bought from traditional market in Surabaya are contaminated by cefotaxime-sensitive Escherichia coli (n=4/4) while chicken meat sample bought from modern market are not contaminated by Escherichia coli (n=0/4). All chicken meat sample bought from traditional (n=4/4) are also contaminated by cefoxitin-sensitive Staphylococcus aureus. Half of chicken meat sample bought from modern market (n=2/4) are contaminated by cefoxitin-sensitive Staphylococcus aureus, while the other half (n=2/4) are contaminated by cefoxitin-resistant Staphylococcus aureus.
Conclusion: Antibiotic resistance is found and all chicken meat samples have been highly contaminated with bacteria therefore food-processing should be done correctly.
Ventola L. The Antibiotic Resistance Crisis. Pharm Ther. 2015;40(4):277–83.
Garedew L, Hagos Z, Zegeye B, Addis Z. The detection and antimicrobial susceptibility profile of Shigella isolates from meat and swab samples at butchers' shops in Gondar town, Northwest Ethiopia. J Infect Public Health. 2016;9(3):348–55
SkoÄková A, KoláÄková I, BogdanoviÄová K, KarpíÅ¡ková R. Characteristic and antimicrobial resistance in Escherichia coli from retail meats purchased in the Czech Republic. Food Control. 2015;47:401–6.
Hughes D, Andersson DI. Environmental and genetic modulation of the phenotypic expression of antibiotic resistance. FEMS Microbiol Rev. 2017;41(3):374–91.
Andleeb S, Majid M, Sardar S. Environmental and public health effects of antibiotics and AMR/ARGs. Antibiotics and Antimicrobial Resistance Genes in the Environment. Elsevier Inc.; 2020. 269–291 p.
Thakare R, Kesharwani P, Dasgupta A, Srinivas N. Antibiotics : Past, Present, and Future [Internet]. Drug Discovery Targeting Drug-Resistant Bacteria. Elsevier Inc.; 2020. 1–8 p.
WHO. Global Antimicrobial Resistance Surveillance System (GLASS) Report. Who. 2017.
Lambrecht E, Van Coillie E, Van Meervenne E, Boon N, Heyndrickx M, Van de Wiele T. Commensal E. coli rapidly transfer antibiotic resistance genes to human intestinal microbiota in the Mucosal Simulator of the Human Intestinal Microbial Ecosystem (M-SHIME). Int J Food Microbiol. 2019;311(August):108357.
Capita R, Riesco-Peláez F, Alonso-Hernando A, Alonso-Calleja C. Exposure of Escherichia coli ATCC 12806 to sublethal concentrations of food-grade biocides influences its ability to form biofilm, resistance to antimicrobials, and ultrastructure. Appl Environ Microbiol. 2014;80(4):1268–80.
Tong SYC, Davis JS, Eichenberger E, Holland TL, Fowler VG. Staphylococcus aureus infections: Epidemiology, pathophysiology, clinical manifestations, and management. Clin Microbiol Rev. 2015;28(3):603–61.
Feltrin F, Alba P, Kraushaar B, Ianzano A, Argudín A, Matteo D. A Livestock-Associated, Multidrug-Resistant, Methicillin-Resistant Staphylococcus aureus Clonal Complex 97 Lineage Spreading in Dairy Cattle and Pigs in Italy. 2016;82(3):816–21.
Velasco V, Buyukcangaz E, Sherwood JS, Stepan RM, Koslofsky RJ, Logue CM. Characterization of staphylococcus aureus from humans and a comparison with isolates of animal origin, in North Dakota, United States. PLoS One. 2015;10(10):1–15.
Swenson JM, Tenover FC, Addison R, D'Souza H, O'Connor J, Rothberg J, et al. Results of disk diffusion testing with cefoxitin correlate with presence of mecA in Staphylococcus spp. J Clin Microbiol. 2005;43(8):3818–23.
Cappuccino JG, Welsh C. Microbiology; A Laboratory Manual. Eleventh. Microbiology. Harlow, Essex, England: Pearson; 2017.
CLSI. Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fifth Informational Supplement. Wayne, PA: Clinical and Laboratory Standards Institute; 2015.
BSN. Standar Nasional Indonesia 7388:2009 Tentang Batas Maksimum Cemaran Mikroba Dalam Pangan. 2009.
Rouger A, Tresse O, Zagorec M. Genetic and nutrition development of indigenous chicken in Africa. Microorganisms. 2017;5(50).
Nyamusore J, Nahimana MR, Ngoc CT, Olu O, Isiaka A, Ndahindwa V, et al. Risk factors for transmission of Salmonella Typhi in Mahama refugee camp, Rwanda: A matched case-control study. Pan Afr Med J. 2018;29:1–13.
Adeyanju GT, Ishola O. Salmonella and escherichia coli contamination of poultry meat from a processing plant and retail markets in Ibadan, Oyo state, Nigeria. Springerplus. 2014;3(1):1–9.
Altalhi AD, Gherbawy YA, Hassan SA. Antibiotic Resistance in Escherichia coli Isolated from Retail Raw Chicken Meat in Taif, Saudi Arabia. Foodborne Pathog Dis. 2010;7(3):281–5.
Yamamoto T, Nishiyama A, Takano T, Yabe S, Higuchi W, Razvina O, et al. Community-acquired methicillin-resistant Staphylococcus aureus: Community transmission, pathogenesis, and drug resistance. J Infect Chemother [Internet]. 2010;16(4):225–54.
Tang Y, Larsen J, Kjeldgaard J, Andersen PS, Skov R, Ingmer H. Methicillin-resistant and -susceptible Staphylococcus aureus from retail meat in Denmark. Int J Food Microbiol [Internet]. 2017;249:72–6.
Neela V, Zafrul AM, Mariana NS, Van Belkum A, Liew YK, Rad EG. Prevalence of ST9 methicillin-resistant Staphylococcus aureus among pigs and pig handlers in Malaysia. J Clin Microbiol. 2009;47(12):4138–40.
Mulders MN, Haenen APJ, Geenen PL, Vesseur PC, Poldervaart ES, Bosch T, et al. Prevalence of livestock-associated MRSA in broiler flocks and risk factors for slaughterhouse personnel in the Netherlands. Epidemiol Infect. 2010;138(5):743–55.
Haaber J, Penadés JR, Ingmer H. Transfer of Antibiotic Resistance in Staphylococcus aureus. Trends Microbiol [Internet]. 2017;25(11):893–905.
Ugwu IC, Anyanwu MU, Ugwu CC, Okoro JC. Isolation and Detection of Methicillin-Resistant Staphylococci in Healthy Broilers in Nsukka Southeast, Nigeria. Not Sci Biol. 2015;7(1):20–5.
Mkize N, Zishiri OT, Mukaratirwa S. Genetic characterisation of antimicrobial resistance and virulence genes in Staphylococcus aureus isolated from commercial broiler chickens in the Durban metropolitan area, South Africa. J S Afr Vet Assoc. 2017;88(1):1–7.
Witte W, Strommenger B, Stanek C, Cuny C. Methicillin-resistant Staphylococcus aureus ST398 in Humans and Animals, Central Europe. Emerg Infect Dis. 2007;13(2):255–8.
van Rijen MML, Kluytmans-van den Bergh MFQ, Verkade EJM, ten Ham PBG, Feingold BJ, Kluytmans JAJW, et al. Lifestyle-Associated Risk Factors for Community-Acquired Methicillin-Resistant Staphylococcus aureus Carriage in the Netherlands: An Exploratory Hospital-Based Case-Control Study. PLoS One. 2013;8(6):1–7.
Verkade E, Kluytmans J. Livestock-associated Staphylococcus aureus CC398: Animal reservoirs and human infections. Infect Genet Evol. 2014;21:523–30.
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