Curcumin Sebagai Photosensitizers Terapi Cahaya LED Biru untuk Penyembuhan Luka Infeksi Secara In Vivo

Juandri S. Tusi

= http://dx.doi.org/10.20473/bsn.v17i3.Y2015.10759
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Abstract


Abstrak

Salah satu penyebab infeksi luka adalah bakteri Staphylococcus Aureus. Penelitian ini bertujuan untuk efektifitas fotoinaktivasi LED biru dengan curcumin untuk penyembuhan luka infeksi secara in vivo. Sampel terdiri dari (a) kontrol negatif, (b) curcumin, (c) LED biru, dan (d) tritmen LED biru dengan curcumin menggunakan dosis energi 10, 20, 30, dan 40 menit untuk melihat parameter yang diamati dengan uji Anova. Hasil penelitian pada mencit menunjukan bahwa presentase kolagen dan fibroblas dari setiap kelompok kontrol adalah kontrol negatif (1) kolagen sebesar 11.84% dan fibroblas 9,35%, LED (2) kolagen sebesar 16.94% dan fibroblas 25,29%, Curcumin (3) kolagen sebesar 13.07% dan fibroblas 24,49%, LED dengan Curcumin 10 menit (4) kolagen sebesar 21.98% dan fibroblas 23,23%, LED dengan Curcumin 20 menit (5) kolagen sebesar 36.72% dan fibroblas 24,17%, LED dengan Curcumin 30 menit (6) kolagen sebesar 45.31% dan fibroblas 26,13%, LED dengan Curcumin 40 menit (7) kolagen sebesar 55.17% dan fibroblas 26,85%. Kesimpulan dari hasil uji Histopatologi dengan control perlakuan curcumin dengan  LED biru 40 menit menghasilkan jumlah kolagen sebanyak 55,17% dan fibroblas 26,85%, sedangkan analisis dengan pengujian secara makroskopik diketahui luka telah sembuh pada hari keenam pada kelompok kontrol tersebut.

Kata kunci : luka infeksi, curcumin, LED biru.


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DAFTAR PUSTAKA

Chattopdhyay, I., Biswas, K., Bandyopdhyay, U., dan Banerje, K.R. 2004. Turmeric and Curcumin: Biological Actions And Medicinal Applications. Current Science 87(1): 44-53

DEPKES RI (2007), Pedoman Manajerial Pencegahan Dan Pengendalian Infeksi Di Rumah Sakit dan Fasilitas Kesehatan Lainnya, Jakarta

Dowshen, et al. 2002. Staphylococcus aureus. http:ud/ac.id/primahapsa/files/ 2012/06/jtptunimus-gdl-primahapsa-5337-1-bab1.pdf.

Grossweiner LI, 2005. The Science of Phototherapy: An Introduction. Springer: USA.

Hamblin MR, Hasan T, 2004. Photodynamic therapy: a new antimicrobial approach to infectious disease? J of Photochemical and Photobiology, 3: 436-450.

Hegge, A.B., Nielsen, T.T., Larsen, K.L., Bruzell, E., Tønnesen, H.H., 2011. Impact of curcumin supersaturation in antibacterial photodynamic therapy (aPDT) –effect of cyclodextrin type and amount. Studies on curcumin and curcuminoides XLV. J. Pharmacology. Sci. http://dx.doi.org/10.1002/jps. 23046.

Hegge, A.B., Schuller, R.B., Kristiansen, S., Tønnesen, H.H., 2008. In vitro release of curcumin from vehicles containing alginate and cyclodextrin. Studies of curcumin and curcuminoides XXXIII. Pharmazie 63, 585–592.

Jawetz, Melnick, Alderberg’s, 2001. Medical Microbiology, McGraw-Hill Companies Inc. 22nd edition, 235-23.

M. Wainwright, “Photodynamic antimicrobial chemotherapy (PACT),” Journal of Antimicrobial Chemotherapy, vol. 42, no. 1, pp. 13–28, 1998.

Niemz, Markolf H. 2007. Laser-Tissue Intractions Fundamentals and Applications Third, Enlarged Edition. Germany: Springer.

Papageorgiu P et al., 2000. Phototherapy with Blue (415 nm) and Red (660 nm) Light in The Treatment of Acne Vulgaris, British Journal of Dermatology.

Prasad, Paras N. 2003. Introduction To Biophotonics. New Jersey: Jhon Wiley & Sons, Inc.

T. Dai, Y.Y. Huang, M.R. Hamblin, Photodynamic therapy for localized infections – state of the art, Photodiag. Photodyn. Ther. 6 (2009) 170–188.

Utama, H. W. (2006). Infeksi nosokomial. Jakarta.

Wainwright M, 2009. Photosensitizers in Biomedicines, John Willey & Sons Ltd.

WHO (2004).Prevention of Hospital Acquired Infection, A Practical Guide, 2 http://www.who.int/research/en/emc, diperoleh 19 maret 2009) nd Edition.

Y. Jiang, A. W. Leung, X. Wang, H. Zhang, and C. Xu, “Effect of photodynamic therapy with hypocrellin B on apoptosis, adhesion, and migration of cancer cells,” International Journal of Radiation Biology, 2014.

Y. Jiang, A. W. Leung, X. Wang, H. Zhang, and C. Xu, “Inactivation of Staphylococcus aureus by photodynamic action of hypocrellin B,” Photodiagnosis and Photodynamic Therapy, vol. 10, no. 4, pp. 600–606, 2013.


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