Spot Urine for Sex Determination Forensic Identifications with Amelogenin Locus and Y chromosomes (DYS 19). Bercak Urin untuk Identifikasi Forensik Jenis Kelamin dengan Lokus Amelogenin dan Y Kromosom (DYS19)
Downloads
Abstract
Background: Amelogenin gene was a single copy gene located in an X chromosome and a Y chromosome. The location of amelogenin gene for identification of sex chromosome has good variability between the form and the shape of the X chromosome and the Y chromosome and between Amelogenin alleles among different populations. Purpose: To prove urine spot examination on the results of the sex determination through Deoxyribo Nucleid Acid (DNA) isolation using amelogenin and Y chromosome loci (DYS19). Methods: Spotting the microscopic examination of urine samples to determine the presence or absence of urethral epithelial cells, followed by isolation Deoxyribo nucleid Acid (DNA) in order to determine the extent and purity of DNA amplification. Then performed Polymerase Chain Reaction (PCR) amelogenin locus at 106bp - 112bp and Y chromosomes (DYS19) at 232 -268 bp. Results: in 9 samples of men from 3 families with 3 kinship of different regions shows the results of different tests, because Amel Y variation between individual and populations method of determining the sex of 100% was inaccurate. In some men Amel Y can be removed entirely. This research should be visualized one band on the Y chromosome (DYS19) and the Amelogenin two bands during electrophoresis occurs misidentification of the sample as a woman. Conclusions: Identification of sex using Amelogenin locus and Y chromosomes (DYS19) has six identical and ambiguous results because the two samples shown as the sign of men but visualized as women, another sample was not visualized because of the thick level and concentration of Deoxyribo nucleid Acid (DNA).
Keywords: Urine Spot, Sex Determination, Amelogenin, Y chromosome (DYS19).
DAFTAR PUSTAKA
Thangaraj K,Reddy AG,Singh L.2002.Apakah gen Amelogenin diandalkan untuk identifikasi jenis kelamin pada identifikasi forensic dan diagnose prenatal Int J Legal Med.116(2). 3-121.
Gatut S and Ujinaka T.2004.Pengaruh waktu terhadap kualitas dan kuantitas DNA Forensik : Sampel Whole Blood tanpa zat antikoagulan dalam bunga rampai Ilmu Kedokteran forensic&Medikolegal,Konas III PDFI Semarang 23-24 Juli 2004
Atmaja D S, 2005. Peranan Sidik jari DNA dalam bidang Forensik, Seminar Nasional Aplikasi DNA Finger Printing dalam bidang kedokteran,29 Agustus 2005, PS UGM.
Lattanzi, W., Di Giacomo, M. C., Lenato, G. M., Chimienti, G., Voglino, G., Resta, N., Pepe, G., Guanti, G. A large interstitial deletion encompassing the amelogenin gene on the short arm of the Y chromosome. Hum. Genet. 116: 395-401, 2005. [PubMed: 15726419, related citations]
Kusuma S E,Yudianto A, Sosiawan A. 2007. Pemeriksaan Identifikasi Forensik Molekuler (panduan laboratorium).Kelompok Studi Human Genetik TDC. Universitas Airlangga.
Syukriani Yoni. 2012. DNA Forensik.Sagung seto.Jakarta
Tilgner, Hagen, Knowles, David G., Johnson, Rory, Davis, Carrie A. Chakrabortty, udipto; Djebali, Sarah; Curado, Joí£o; Snyder, Michael, Gingeras, Thomas R. Guigó, Roderic. 2012 . "Deep sequencing of subcellular RNA fractions shows splicing to be predominantly co-transcriptional in the human genome but inefficient for lncRNAs". Genome Research 22 (9): 1616–25.
Authors who publish with this journal agree to the following terms:
- The copyright of this journal belongs to the Editorial Board and Journal Manager with the author's knowledge, while the moral right of the publication belong to the author.
- The formal legal aspect of journal publication accessibility refers to the Creative Commons Attribution-Share Alike (CC BY-SA).
- Every publication (print/electronic) is open access for educational, research, and library purposes. In addition to the objectives mentioned above, the editorial board is not responsible for copyright infringement
