Identifikasi KHV dengan Uji Immunofluorescence dan Immunocytochemistry Berdasarkan Uji Polymerase Chain Reaction Positif KHV pada Ikan Koi (Cyprinus carpio)
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Koi Herpesvirus (KHV) menyebabkan penyakit parah dan kematian di segala usia ikan mas dan ikan koi (Cyprinus carpio) dan menyebar dengan cepat di seluruh dunia sebagai penyebab mortalitas yang sangat tinggi dengan perkiraan (80-95%) untuk ikan koi dan ikan mas. Identifikasi KHV ini sudah dilakukan dengan metode Polymerase Chain Reaction (PCR). Tetapi, metode tersebut tidak cocok untuk pekerjaan lapangan karena belum efisien dalam hal waktu dan peralatan. Perlu ada pemeriksaan berbasis imunologis sebagai identifikasi awal (pemeriksaan dini) yang belum banyak dilakukan dan perlu dikembangkan terutama untuk pemeriksaan KHV yaitu metode Immunofluorescence sebagai pemeriksaan imunologis untuk identifikasi adanya antigen atau dengan menggunakan pewarnaan fluorescent dan immunocytochemistry menggunakan metode imuno histo (sito) kimia ensim yang bersifat spesifik dengan pewarnaan oleh zat chromogen guna mengidentifikasi antigen dalam suspensi organ insang dan darah. Tujuan dari penelitian ini adalah agar mampu mengidentifikasi KHV (Koi Herpes Virus) yang menginfeksi ikan koi (Cyprinus carpio L) menggunakan metode aplikasi Immunofluorescence serta Immunocytochemistry berdasarkan pemeriksaan menggunakan uji PCR dengan hasil positif KHV.Hasil dari kedua metode diperoleh positif untuk beberapa sampel berdasarkan positif PCR, ditandai dengan adanya pendaran oleh zat fluorescein berwarna hijau pada antigen KHV dengan pemeriksaan metode Imunofluorescence dan adanya ekspresi zat chromogen pada antigen berwarna coklat kemerahan pada antigen KHV dengan pemeriksaan Imunocytochemistry. Sehingga dapat disimpulkan bahwa kedua metode tersebut mampu mengidentifikasi KHV dan dapat digunakan sebagai uji pemeriksaan dini KHV berbasis imunologis.
Kata kunci: Koi Herpesvirus, Ikan koi (Cyprinus carpio), Imunofluorescence, Immunocytochemistry
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