Potensi Jatropha multifida Terhadap Jumlah Fibroblast pada Aphthous Ulcer Mukosa Mulut Tikus
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Peningkatan pemakaian obat dengan kandungan asli untuk lebih bebas efek toksik, mudah didapat dan harga yang terjangkau telah menjadi kebutuhan masyarakat dewasa ini. Jatropha multifida, dengan kandungan bioaktif alkaloid, flavonoid, saponin, tanin, dan diterpenoid berperan dalam penyembuhan luka. Tujuan penelitian untuk mengetahui potensi ekstrak Jatropha multifida terhadap jumlah fibroblast pada proses penyembuhan aphthous ulcer atau sariawan yang sering terjadi di masyarakat. Subyek penelitian adalah 24 ekor tikus Rat norvegicus dibagi menjadi kelompok K0 (CMC-Na), P1 (JM 2,5%); P2 (JM5%), P3(JM10%). Perlukaan pada mukosa mulut tikus dengan memakai burnisher yang dipanaskan dan ekstrak J.Multifida diberikan secara topikal 2 kali sehari selama 5 hari. Tikus dikorbankan dan dibiopsi incise pada hari ke-5. Untuk melihat jumlah fibroblast dilakukan pembuatan preparat dengan pengecatan Hematoxylin Eosin. Hasil: Terdapat perbedaan jumlah fibroblast secara signifikan pada hari ke-5 di K0 (55,70), P1(69,60), P2(48,20), P3(65,53). Kesimpulan: ada perbedaan bermakna antara kelompok kontrol, P1 dan P2 dengan P3. Ektrak gel Jatropha multifida 5% paling efektif terhadap jumlah fibroblast pada proses penyembuhan mukosa mulut tikus rat norvegicus.
Kata kunci : Aphthous ulcer, Jatropha multifida, fibroblast
DAFTAR PUSTAKA:
Adesola A, Adetunji O., 2007.Tersedia dalam jurnal Ekstraksi zat aktif antimikroba dari tanaman yodium (Jatropha Multifida Linn) sebagai bahan baku alternatif antibiotik alami oleh Sari FM&Sari SM. hal:1
Anabel Diaz et al., 2015. Behaviour of The Recurrent Aphthous Stomatitis as a Dental Urgency at Vedado's University Policlinic. Faculty of Dental Medicine, University of Porto Portugal Original Research.
Al Samadi et al., 2015. Epithelial Cell Apoptosis in Recurrent Aphthous Ulcer. Journal of dental research. Vol.94 (7) 928-935. Institute of Clinical Medicine Biomedicum, Helsinki, Finland.
Anthony L. Mescher, 2013. Basic Histology Text and Atlas. Thirteenth edition. Medical McGraw Hill eBook. Indiana
Beatrize C.K. Hirota et al., 2012. C- Glycosyl Flavones and Comparative Study of The Antioxidant, Haemolytic and Toxic Potential of Jatropha multifida Leaves and Bark. International Journal of Phytomedicine 4. 01 – 05. Brazil.
Christian Agyare, Yaw Duah Boakye, 2015. African Medicinal Plants With Wound Healing Properties. Journal of Ethnopharmacology Institute for Pharmaceutical Biology and Phytochemistry, University of Munster, Germany.
E. J. Mazyala, 2008. Dermal Fibroblast : A Histological And Tissue Culture Study. Division of anatomy and histology, faculty of health science, University of Stellenbosch, Mwanza, Tanzania.
Hannu Larjava, 2012. Oral Wound Healing : Cell Biology and Clinical Management, Faculty of Dentistry, University of British Columbia, Vancouver – Canada.
Ian A. Darby, Tim D. Hewitson, 2007. A Survey of Cell Biology : Fibroblast Differentiation in Wound Healing and Fibrosis. The Royal Melbourne Hopital and Department of Medicine.
Laurel M. Morton, Tania J. Phillip , 2016. Wound Healing and Treating Wounds. Differential diagnose and evaluation of chronic wounds. University School of Medicine, Boston. American Academy of Dermatology.
Matthias Chiquet, Christos Katsaros, Dimitris Kletsas, 2015. Multiple Function of Gingival and Mucoperiostal Fibroblasts in Oral Wound Healing and Repair. Periodontology 2000, Vol. 68, 2015, 21- 40. John Wiley & Sons, Singapore.
Nwokocha, Blessing A, Agbagwa IO and Okoli BE., 2011. Comparative Phytochemical Screening of Jatropha L. Species in the Niger Delta.Research Journal of Phytochemistry. 5(2): 107-114
Nicholas S. Greaves et al., 2013. Current Understanding of Molecular and Cellular Mechanism in Fibroplasia and Angiogenesis During Acute Wound Healing. Journal of Dermatological Science – Elsevier.
P. Bainbridge, 2014. Wound Healing and The Role of Fibroblast. Journal of Wound care. Vol 22 no 8, 407 – 8/ 410-12. Biomedical Chemistry, Cardiff University, UK.
Ronald G. Shashy, Marion B. Ridley, 2000. Aphthous Ulcer : A difficult Review Article. Clinical entity Am.J. Otolaryngol 2000; 21: 389-393. Saunders Company.
Salkutty Thomas, 2016. Pharmacognostic and Phytochemical Constituent of Leaves of Jatropha multifida Linn and Jatropha podagrica Hook. Journal of Pharmacognosy and Phytochemistry 2016; 5 (20: 243-246. India.
Sean E.Gill, William C. Parks, 2008. Metalloproteinases and Their Inhibitor: Regulator of Wound Healing. Int. J. Biochem Cell Biol. 2008; 40 (6-7): 1334 – 1347. University of Washington, Seattle, WA.
Silma Rampadarath, Daneshwar Puchoa, Vijayanti Mala, 2014. Antimicrobial, Phytochemical and Larvicidal Properties of Jatropha multifida Linn. Asian Pasific Journal of Tropical Medicine. Faculty of Agriculture, University of Mauritius – Elsevier.
S. Kosasih et al., 1989. Labaditin, A Novel Cyclic Decapeptide from The Latex of Jatropha multifida. Vol 256, number 1,2, 91-96. Elsevier Science Publisher Biomedical Division.
Sophie Van Lithout, Kapka Miteva, Carsten Tschope, 2014. Crosstalk Between Fibroblast and Inflammatory Cells. Cardiovascular Research (2014) 102, 258-259. European Society of Cardiology.
Stephen T. Sonis, 2015. Genomic, Personalized Medicine and Oral Disease. Springer International Publishing Switzerland, 2015.
Sule Coskun, Emine Gulceri Gulec, Barbaros Balabanli, 2007. Effect of Epidermal Growth Factor on Lipid Peroxidation and Nitric Oxide Level in Oral Mucosal Ulcer Healing. A time course study Surg today (2007) 37; 570-574 Gazi University, Ankara – Turkey.
Sunday O. Akintoye, Greenberg MS., 2014. Reccurrent Aphthous Stomatitis. Dent Clin N. Am 2005;49;31-47. School of Dental Medicine, University Of Pennsylvania, Philadelphia.
Shruti Shukla et al.,2012. Plants as Potential Sources of Natural Immuno modulators. Rev.Environ Sci Biotechnol, Mini review. Springer, 2012.
T. Velnar, T. Bailey, V. Smrkolj, 2009. The Wound Healing Process : An Overview of The Cellular and Molecular Mechanism. Journal of International Medical Research 2009 37: 1528. Published by SAGE.
Xiao Wen Jiang et al., 2013. Double Blind, Randomized, Controlled Clinical Trial of The Effects of Diosmetite and Basic Fibroblast Growth Factor Paste On Treatment of Minor Recurrent Aphthous Stomatitis. Oral Surg Oral Med Oral Pathol Oral Radiol 2013; 116:570-575. University Of South China, Chenzou, Hunan, China.
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