Kadar Malondialdehid tikus model Sindroma Ovarium Polikistik dengan daun kelor (Moringa oleifera)
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Sindroma Ovarium Polikistik (SOPK) merupakan endokrinopati paling umum pada perempuan yang sering dikaitkan dengan resistensi insulin, hyperandrogenemia, peradangan kronis, dan oxydative stress. Tingkat oxydative stress pada SOPK diamati secara signifikan berkorelasi dengan obesitas, bahkan ditemukan memainkan peran penting dalam patogenesis kanker. Antioksidan adalah substansi yang diperlukan tubuh untuk menetralisir radikal bebas, penggunaan kelor (Moringa Oleifera) sebagai antioksidan perlu diteliti sebagai alternatif pengobatan terhadap oxydative stress pada SOPK dengan resistensi insulin. Penelitian eksperimen ini menggunakan tikus Rattus norvegicus strain wistar betina berusia 3 bulan dengan berat 100-130 gram dibagi menjadi 4 kelompok (n=8). Tikus model SOPK diberikan injeksi testosteron propionat sebesar 1 mg/100grBB secara intramuskular selama 28 hari, selanjutnya diberikan ekstrak daun kelor (Moringa Oleifera) dengan dosis 250 dan 500 mg/KgBB selama 14 hari. Kemudian dilakukan pengukuran berat badan dan pemeriksaan kadar Malondialdehid (MDA) dalam darah. Hasil penelitian menunjukan bahwa kadar MDA pada kelompok kontrol SOPK naik secara signifikan (p<0.05) dibandingkan dengan kontrol normal. Pemberian ekstrak daun kelor (Moringa Oleifera) 500 mg/KgBB menunjukan penurunan yang signifikan (p<0.05) terhadap kadar MDA dibanding kelompok kontrol SOPK. Pemberian ekstrak daun kelor (Moringa Oleifera) sebagai antioksidan terbukti dapat menurunkan kadar MDA tikus betina model SOPK dengan resistensi insulin.
Kata kunci: Kadar Malondialdehid, ekstrak daun kelor (Moringa Oleifera), Sindroma Ovarium Polikistik
DAFTAR PUSTAKA
Adewole, S. O., Caxton-Martins, E. A. & Ojewole, J. A. 2007. Protective effect of quercetin on the morphology of pancreatic β-cells of streptozotocin-treated diabetic rats. African Journal of Traditional, Complementary and Alternative Medicines, 4, 64-74.
Agarwal, A., Prabakaran, S. A. & Said, T. M. 2005. Prevention of oxidative stress injury to sperm. Journal of Andrology, 26, 654-660.
Balen, A. H., Conway, G. S., Homburg, R. & Legro, R. S. 2005. Polycystic ovary syndrome: a guide to clinical management, CRC Press.
Bostancı, S. M., Bayram, M., Sevinç, F. C., Paşaoğlu, H. & Elbeğ, Åž. 2012. The relationship between biochemical parameters, interleukin-6 and ovarian morphology in polycystic ovary syndrome. Journal of Clinical and Experimental Investigations, 3.
Fahey, J. W. 2005. Moringa oleifera: A Review of the Medical Evidence for Its Nutritional, Therapeutic, and Prophylactic Properties. Part 1. Trees for life Journal, 1.
Fauser, B. C., Tarlatzis, B. C., Rebar, R. W., Legro, R. S., Balen, A. H., Lobo, R., Carmina, E., Chang, J., Yildiz, B. O. & Laven, J. S. 2012. Consensus on women's health aspects of polycystic ovary syndrome (PCOS): the Amsterdam ESHRE/ASRM-Sponsored 3rd PCOS Consensus Workshop Group. Fertility and sterility, 97, 28-38. e25.
Gopalakrishnan, L., Doriya, K. & Kumar, D. S. 2016. Moringa oleifera: A review on nutritive importance and its medicinal application. Food Science and Human Wellness, 5, 49-56.
Homburg, R. 2003. The management of infertility associated with polycystic ovary syndrome. Reproductive Biology and Endocrinology, 1, 109.
Homburg, R. 2008. Polycystic ovary syndrome. Best Practice & Research Clinical Obstetrics & Gynaecology, 22, 261-274.
Jackson, M. J. 2005. Reactive oxygen species and redox-regulation of skeletal muscle adaptations to exercise. Philosophical Transactions of the Royal Society B: Biological Sciences, 360, 2285-2291.
Johnson, B. 2005. Clinical perspectives on the health effects of Moringa oleifera: A promising adjunct for balanced nutrition and better health. KOS health publications.
Kusumastuty, I. & Ratnawati, R. 2013. Epigallocatechin Gallate Green Tea GMB4 Clon Prevent Insulin Resistance due to High Fat Diet in Rat. Jurnal Kedokteran Brawijaya, 26, 63-68.
Marshall, K. 2001. Polycystic ovary syndrome: clinical considerations. Alternative Medicine Review, 6, 272-272.
Mbikay, M. 2012. Therapeutic potential of Moringa oleifera leaves in chronic hyperglycemia and dyslipidemia: a review. Frontiers in pharmacology, 3.
Mukherjee, S. & Maitra, A. 2010. Molecular & genetic factors contributing to insulin resistance in polycystic ovary syndrome.
Oparinde, D. P. & Atiba, A. S. 2014. Moringa oleifera Leaf Prevents Oxidative Stress in Wistar Rats.
Powers, S. K. & Jackson, M. J. 2008. Exercise-induced oxidative stress: cellular mechanisms and impact on muscle force production. Physiological reviews, 88, 1243-1276.
Purwanto, B., Sudiana, I. K., Herawati, L. & Aksono, B. 2013. MUSCLE GLUCOSE TRANSPORTER 1 (GLUT-1) EXPRESSION IN DIABETIC RAT MODELS. Folia Medica Indonesiana, 49, 21-25.
Rojas, J., Chávez, M., Olivar, L., Rojas, M., Morillo, J., Mejías, J., Calvo, M. & Bermúdez, V. 2014. Polycystic ovary syndrome, insulin resistance, and obesity: navigating the pathophysiologic labyrinth. International journal of reproductive medicine, 2014.
Roloff, A., Korn, S. & Gillner, S. 2009. The climate-species-matrix to select tree species for urban habitats considering climate change. Urban Forestry & Urban Greening, 8, 295-308.
Rusnasari, V. D. 2005. HUBUNGAN RESISTENSI INSULIN (HOMA-IR) DENGAN OBESITAS DAN PERUBAHAN HORMON ANDROGEN PADA PENDERITA SINDROMA OVARIUM POLIKISTIK. Program Pasca Sarjana Universitas Diponegoro.
Sikka, S. C., Rajasekaran, M. & Hellstrom, W. J. 1995. Role of oxidative stress and antioxidants in male infertility. Journal of andrology, 16, 464-468.
Slowey, M. J. 2001. Polycystic ovary syndrome: new perspective on an old problem. Southern medical journal, 94, 190-196.
Sujatha, B. & Patel, P. 2017. Moringa Oleifera–Nature's Gold. Imperial Journal of Interdisciplinary Research, 3.
Tan, H. 2011. Perbandingan Efektifitas dan Efek Samping Pemakaian Metformin XR dan Metformin IR dalam Pengobatan PCOS yang Resisten terhadap Clomiphene Citrate.
Teede, H. J., Misso, M. L., Deeks, A. A., Moran, L. J., Stuckey, B., Wong, J., Norman, R. J., Costello, M. F. & Guideline, D. G. 2011. Assessment and management of polycystic ovary syndrome: summary of an evidence-based guideline. The Medical Journal of Australia, 195, S65.
Touyz, R. M. 2005. Molecular and cellular mechanisms in vascular injury in hypertension: role of angiotensin II–editorial review. Current opinion in nephrology and hypertension, 14, 125-131.
Valkenburg, O., Steegers-Theunissen, R. P., Smedts, H. P., Dallinga-Thie, G. M., Fauser, B. C., Westerveld, E. H. & Laven, J. S. 2008. A more atherogenic serum lipoprotein profile is present in women with polycystic ovary syndrome: a case-control study. The Journal Of Clinical Endocrinology & Metabolism, 93, 470-476.
Winarsi, H. 2005. Antioksidan Alami dan Radikal, Kanisius.
Yeon Lee, J., Baw, C.-K., Gupta, S., Aziz, N. & Agarwal, A. 2010. Role of oxidative stress in polycystic ovary syndrome. Current Women's Health Reviews, 6, 96-107.
Ziech, D., Franco, R., Pappa, A. & Panayiotidis, M. I. 2011. Reactive Oxygen Species (ROS)––Induced genetic and epigenetic alterations in human carcinogenesis. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 711, 167-173.
Zuo, T., Zhu, M. & Xu, W. 2015. Roles of oxidative stress in polycystic ovary syndrome and cancers. Oxidative medicine and cellular longevity, 2016.
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