Jurnal Biosains Pascasarjana

DETERMINATION OF DIASTASE ENZYME ACTIVITY AND WATER CONTENT OF HONEY FOREST OF SULA ISLAND, NORTH MALUKU

2025-06-24T18:04:36+07:00

The experiment that has been carried out aims to determine the quality of forest honey originating from the Sula Islands, North Maluku based on the parameters set by the Indonesian National Standards Agency (SNI) 8864:2018, which includes organoleptic parameters (odour and taste), water content and Diastase Number. These parameters can indicate the originality of a forest honey product. The research was conducted at the Forensic Laboratory Center of the Criminal Investigation Department of the Republic of Indonesia Police, using the laboratory experiment method, using the UV-Vis Spectrophotometry instrument, forest honey originating from the Sula Islands district, North Maluku Province was used as material in this experiment. Organoleptic tests including odour and taste were carried out using the sense of smell and taste, water content testing using an oven drying technique, and DN value determination using the UV-Vis Spectrophotometry instrument. The experiment results showed that some parameters met the requirements and did not meet the criteria. Tests on Sula Islands forest honey's taste and water content parameters did not meet the criteria, but the odour and DN parameters met the requirements.

Keywords: Forest honey, honey quality, UV-Vis spectrophotometry

COMPARISON OF 10% BUFFERED FORMALIN NEUTRAL FIXATION SOLUTION WITH BOUIN ON MICROSCOPIC IMAGES OF CHICKEN HEPAR AND CEREBRUM WITH HEMATOXYLIN EOSIN (HE) STAINING

2025-05-15T14:31:55+07:00

The fixation process is an important stage in the manufacture of histopathology preparations. Fixation aims to prevent autolysis and tissue degradation, so that the results can be observed both anatomically and microscopically. Fixation solutions that are often used in routine histopathological examinations are 10% Formalin Buffer Neutral and Bouin. This study was used to determine the results of the comparison of 10% NBF fixation solution with Bouin on the microscopic picture of hepar and cerebrum with hematoxylin eosin staining. The type of research used is a cross sectional approach with a qualitative descriptive design. Sampling was performed on chicken animals. The samples used were hepar and cerebrum fixed with 10% NBF and 10% Bouin for 12 hours, stained with Hematoxylin Eosin and observed microscopically. Then give an assessment with good, less good, or not good categories based on assessment indicators based on cell color and shape. The results showed a microscopic picture of hepatic and cerebrum tissue as much as 6 preparations fixed with 10% NBF liquid showed good results. While hepatic and cerebrum tissue fixed with bouin liquid as many as 6 preparations showed poor results. The conclusion is that there are differences in the microscopic results of hepatic and cerebrum tissue fixed with 10% NBF solution and Bouin. Good microscopic observation results based on the assessment indicator criteria are tissues fixed with 10% NBF liquid.

REVIEW OF THE USE OF DNA BARCODING IN AVIFAUNA RESEARCH: CONSERVATION, AUTHENTIFICATION, AND DISEASE DETECTION

2025-05-15T14:51:24+07:00

DNA barcoding is an effective tool for identifying species, with additional uses for measuring molecular diversity, authentication of illegal products, and early detection of disease types. In this review, we discussed the use of DNA barcoding for Avifauna research, that is known to have an important impact on environmental health. The existence of Avifauna is threatened with extinction due to human anthropogenic activities. This review starts by providing a general overview of DNA barcoding, focusing on its application in Avifauna research, metabarcoding, as a tool in detecting food samples from the Avifauna, tracking enandgered and protected exotic birds, and tracking avifauna diseases. This review concludes with definitive statements and challenges regarding the use of DNA barcoding, especially in regions with high levels of avifauna diversity. This review can contribute to a better understanding of DNA barcoding and its potential in managing Avifauna genetic resources in the wild and conservation institutions.

ANTI-INFLAMMATORY POTENTIAL OF FUNGUS COMBS FROM INDO-MALAYAN TERMITE MACROTERMES GILVUS HAGEN MOUND: REVIEW ARTICLE

2025-05-15T14:49:55+07:00

There is evidence that termite colonies of the subfamily Termitomyces produce an unusual structure called a fungus comb, especially Termite mounds in Indo-Malaysian regions. This comb may include bioactive components that the termites use to investigate if they might aid in the body's inflammatory response. The anti-inflammatory potential, concentration, and bioavailability of fungal comb from the Indo-Malayan termite macrotermes gilvus Hagen mound will be further discussed in this research. We utilized databases to review the current evidence related to anti-inflammatory potential of fungus combs and termite macrotermes gilvus hagen mound. We discussed the most recent evidence of fungus combs and termite macrotermes gilvus mound regarding the anti-inflammatory potential, concentration, and bioavailability. This study concludes that ethyl acetate extract from fungus comb could enhance the immune system. Phenolic compounds have a positive effect on health indicators. These mechanisms may account for the decreased prevalence of chronic degenerative disorders in populations.

THE EFFECT OF LED MAGNETIC ON STAPHYLOCOCCUS AUREUS BACTERIA

2025-05-15T14:48:36+07:00

Nosocomial infections, caused by viruses, bacteria, and fungal pathogens, often occur during treatment in health facilities due to cross-contamination from healthcare workers or medical equipment. The study explores the efficacy of static magnetic fields and photodynamic inactivation in inactivating Staphylococcus aureus bacteria, a crucial step in sterilization. The study used high-power blue LEDs and static magnetic fields generated by neodymium magnets. The highest reduction percentage observed was 81.92 ± 7.92%, found in the combination treatment of static magnetic fields (SMF) with LED illumination at the F8 microplate location, with a treatment time of 40 minutes, an LED dose of 11.72 J/cm², and a magnetic field dose of 25.61 mT. The lowest reduction percentage, 52.41 ± 7.64%, was observed at the F8 microplate location with a treatment time of 10 minutes, an LED dose of 2.93 J/cm², and a magnetic field dose of 25.61 mT.

ANALYSIS OF REPRODUCTIVITY OF MADRASIN CATTLE BEFORE AND AFTER FOOT AND MOUTH DISEASE ON GILI IYANG ISLAND, SUMENEP, MADURA

2025-05-15T14:39:39+07:00

This research aims to analyze reproductivity (S/C and Pregnancy) and supply and demand for Madrasin cattle before and after FMD disease on Gili Iyang Island, Sumenep, Madura. This research used data from 450 madrasin cattle on Gili Iyang Island, Sumenep. The research was carried out by comparing data obtained before the FMD outbreak in January 2022 – May 2022 and after the FMD outbreak in June 2022 – November 2022. The results of the research showed that the reproductivity of Madrasin cattle before and after the FMD disease on Gili Iyang Island, Sumenep, Madura in S/C increased, while during pregnancy there is a decrease. The supply and demand for the Madrasin cattle business before and after the FMD on Gili Iyang Island, Sumenep, Madura, decreased.

ANALYSIS OF THE IMPACT OF FOOT-AND-MOUTH DISEASE ON PRODUCTION MANAGEMENT AND BUSINESS FEASIBILITY OF SAPI SONOK IN SUMENEP REGENCY

2024-08-09T13:08:27+07:00

This study aims to determine the impact of foot and mouth disease (FMD) on production management consisting of maintenance management, cage management, health management, feed management and business analysis of sonok cattle in Potre Koning Sonok Cattle Association in Lenteng Sub-district, Sumenep District. This study used 21 respondents consisting of 20 association members and one paramedic. The primary and secondary data were obtained from the distribution of questionnaires and interviews. Questionnaire data are divided into three groups: before FMD, during FMD, and post-FMD, which were then analyzed using the Manova test and the Duncan test. The results showed significant differences in housing management, health management, feed management, and business analysis (p<0.05). Housing management showed no significant difference in the time before, during and after FMD, while business analysis during FMD suffered losses due to decreased productivity of sonok cattle. Health management is the most influential management during FMD. Farmers ignore the profitability of raising sonok cattle because sonok cattle are a hobby for farmers.

PHYSICS OF BLOODSTAIN PATTERN ANALYSIS

2025-05-15T14:36:41+07:00

Bloodstain patterns are one of the pieces of evidence that can be found at the scene. Bloodstain pattern analysis is carried out to help reconstruct the events or occurrences that caused the formation of the pattern. Analysis is based on a combination of physics, biology and mathematics. Factors that influence the formation of bloodstain patterns are: surface tension and viscosity, drag and gravity and their influence on speed, and the shape of bloodstains when falling. In addition, surface conditions can affect the pattern of blood produced. Determining the area of "‹"‹origin of the bloodstain is carried out based on the impact angle calculated from the length and width of the bloodstain pattern. The analysis carried out in the experiment shows that there is closure in the bloodstain pattern analysis due to the assumption of a linear trajectory and determining the angle of the bloodstain. Different impact speeds will produce different bloodstain patterns.

A REVIEW: EXPERIMENTAL ANIMAL MODEL OF HELICOBACTER PYLORI IN 2018-2023

2025-05-15T14:34:27+07:00

H. pylori is a Gram-negative bacterium that has colonized the stomachs of approximately 50% of the human population worldwide. H. pylori is a major pathogen associated with gastrointestinal diseases, including gastritis, peptic ulcers, and gastritis, gastric adenocarcinoma, and mucosa-associated lymphoid tissue (MALT). To study the pathogenesis, prevention and therapy of H. pylori infection, ideal, safe and stable animal model experiments are needed. Therefore, in this text we summarize several important aspects in successfully creating experimental animal models of H. pylori. The method used is literature study through two electronic databases, namely Sciencedirect and Pub Med. A total of 50 articles were used in this study. Full text assessment was carried out according to inclusion and exclusion criteria. The C57BL/6 and BALB/c animal models are capable of producing gastritis, metaplasia and hyperplasia and rarely produce cancer, while Mongolian gerbils and transgenic mice are considered more susceptible to creating cancer models. The strains commonly used in research on H. pylori infection are SS1 and PMSS1 because they are considered to be CagA-positive. The non-invasive method for confirming H. pylori that is most often used in experimental animal models is the ELISA serological test, while the invasive methods that are often used are RUT and H&E and other additional staining.

ALTERNATIVE MATRICES FROM THE ORAL CAVITY AS A SOURCE FOR DRUG DETECTION

2025-06-10T13:55:18+07:00

Forensic toxicology plays a crucial role in law enforcement by detecting xenobiotic substances in the human body, aiding in legal decisions. Traditional matrices like blood and urine have significant drawbacks, such as invasiveness, susceptibility to adulteration, and limited detection windows. This paper explores alternative matrices from the oral cavity, including oral fluid, teeth, dental plaque, and dental calculus, for drug detection. Oral fluid offers non-invasive collection and reliable pharmacokinetic data, while teeth provide long-term drug detection in postmortem cases. Dental plaque and dental calculus can trap substances over time, offering insights into past drug use with less invasive sampling. These matrices reflect the overall condition of the body and provide valuable forensic information. Continued research and technological advancements are necessary to optimize their application in various forensic scenarios.