Jurnal Biosains Pascasarjana

LASERPUNCTURE TECHNOLOGY AS AN EFFORT PREPARATION OF SIAM CATFISH SEED (Pangasianodon hypophthalmus) MASS

Maulia Patmadevi, Ahmad Taufiq Mukti, Ahmad Shofy Mubarak, Suryani Dyah Astuti — Mon, 01 Jul 2024 00:00:00 +0700

The spawning of striped catfish which is very dependent on the season and environmental conditions results in the unavailability of mature gonad broodstock during the dry season. The alternative that is often done is by biological stimulation using the ovaprim hormone and laserpuncture technology. The purpose of this study was to determine the effect of giving the ovaprim hormone, laserpuncture induction and a combination of the two on the speed of spawning time and the fecundity value of Striped catfish. This study used an experimental method with a completely randomized design with 3 treatments and 6 replications. The results of this study were the average spawning time for broodstock treated with laserpuncture and ovaprim hormone and the fecundity value was significantly different from the other treatments (p<0.05). Based on the results of the study, it can be concluded that the combination of 0.5 ml/kg of ovaprim hormone and laser puncture at a dose of 0.5 J/cm² for 32.66 seconds can speed up the spawning time and fecundity of striped catfish.

MORINGA OLEIFERA EXTRACT CAN INHIBIT GLOMERULUS DAMAGE OF RATTUS NOVERGICUS INJECTED CYCLOPHOSPHAMIDE

Laurencia Dwiamanda Elizabeth, Theresia Indah Budhy, Retno Pudji Rahayu — Mon, 01 Jul 2024 00:00:00 +0700

Cyclophosphamide is a cytostatic used in chemotherapy. Cyclophosphamide has a toxic effect that causes damage to the kidneys. Moringa oleifera is a plant with high antioxidant activity. Its antioxidant content in the form of flavonoids and vitamin C which can inhibit glomerulus damage. Many studies have proven that Moringa oleifera contains high antioxidant activity. However, there has been no research about the potential of Moringa oleifera in inhibiting glomerulus damage that was injected with cyclophosphamide. The purpose of this study was to determine the potential of Moringa oleifera in inhibiting glomerulus damage in Rattus novergicus due to cyclophosphamide injection. This was a laboratory experiment with a post-test only control group design. This study used 18 Rattus norvegicus as samples which was divided into 3 sample groups namely K1 is the group that was only given NaCl, K2 was the group that was given NaCl and injected by cyclophosphamide, and the treatment group was given Moringa oleifera extract. Glomerulus histopathological preparations were stained with Hematoxylin Eosin. Data were analyzed statistically by the Kruskall Wallis and Mann-Whitney tests. The Mann-Whitney test showed significant difference between groups K1 and K2 and groups of P and K2 (p<0.05). There were no significant differences between K1 and P (p>0.05). Giving Moringa oleifera extract can inhibit the glomerular damage of Rattus novergicus, which is injected with cyclophosphamide.

EFFECT OF DIFFERENCES BROMELAIN ENZYME CONSENTRATION ON PROTEIN HYDROLYSATE FROM WASTE OF TILAPIA VISCERA (Oreochromis sp.) ON ANTIOXIDANT ACTIVITY

Muhammad Athoillah Sholahuddin, Nunuk Dyah Retno Lastuti, Muhamad Amin — Mon, 01 Jul 2024 00:00:00 +0700

Tilapia is a commodity that is widely consumed by the public, especially in Indonesia. Fresh tilapia offal has a protein content composition of 55.55%, so to increase its selling value it is often made into protein hydrolysate. Protein hydrolysis using enzymes is an effective way, because it can make protein hydrolysates avoid damage to certain amino acids. Proteolytic enzymes have many types and one that can be used as a hydrolyzer, in producing protein hydrolysates is bromelain enzyme. The purpose of this study was to determine the effect of proteolytic enzyme type and hydrolysis time on antioxidant activity of protein hydrolysate from Oreochromis sp. offal waste. This study used RAL 2 factors and each was repeated 3 times. The first factor was the concentration of bromelain enzyme used, namely control concentration, 1%, 2% and 3%. The second factor was the hydrolysis time, which was 4 hours, 6 hours and 8 hours. The data obtained were analyzed and continued with antioxidant activity test, hydrolysis degree test and protein content test. The best concentration of bromelain enzyme as antioxidant was 3% concentration for 6 hours hydrolysis with IC50 value of 82.53µg/mL, the best DH of 82.25%±4.03 and the highest protein content of 54.55%.

Keywords: Antioxidant, bromelain enzyme, tilapia offal, protein hydrolysate

TEICHMANN TEST: ASSESSMENT OF HEMOGLOBIN CRYSTALS ON BLOOD SPOTS EXPOSED TO POWDER DETERGENT

Istiana Firqah Abid, Erik Rezakola, Imam Yusuf Hanura — Mon, 01 Jul 2024 00:00:00 +0700

Evidence found at the crime scene (TKP) in the form of bloodstains needs to be examined to ensure that the evidence is really blood so that it can assist the investigation process. However, in some cases, criminals can remove or remove bloodstains using detergents that are used daily which contain surfactants. The purpose of this study was to find out how to examine and describe hemoglobin crystals in blood spots exposed to several powder detergents using the Teichmann test. This study used an experimental method using 3 different brands of detergent with 2 controls, namely a positive control with the presence of blood spots and a negative control without blood spots. The examination results obtained positive control results in all experiments as much as 100% still giving a brown rhombus-shaped appearance of hemoglobin crystals like needles even though the blood spots had been exposed to powder detergent.

Keywords: Blood spot, Detergent, Hemoglobin, Teichmann test

D-DIMER AS A PROGNOSIS OF THE SEVERITY OF COVID-19 PATIENTS AT BEKASI REGENCY HOSPITAL

Dyah Eka Kurniawati — Mon, 01 Jul 2024 00:00:00 +0700

The increase in D-dimer is the most significant change in coagulation parameters in Covid-19 patients and can be a marker of poor prognosis. The purpose of this study was to see the average significant D-dimer levels in patients with confirmed Covid-19 with severity. The method used is Retrospective Research with a purposive sampling technique based on the criteria of the severity of Covid-19 disease. Patient data was taken from medical records and D-dimer levels from the Laboratory Information System and then analyzed using a one-way Anova test. The results showed an average D-dimer level of 2.66 mg/L at critical degrees, 2.02 mg/L at severe degrees, 0.30 at moderate degrees and 0.26 at mild degrees. Male patients occupy a larger percentage than female patients with a total of 62.5%. The age of 46-65 years occupies the highest percentage of 55% for the abnormal category because the immune system as a protector of the body does not work as strongly as when it was young. This study showed a significant average difference in the groups of mild severity with severe, mild with critical, moderate with severe, and moderate with critical. For the next researcher, it is expected to be able to analyze other hemostasis examination parameters with variables of disease severity.

Kata kunci: Coronavirus, COVID-19, D-dimer, parameter koagulasi, pembekuan darah

EFFECT OF HIGH-FAT DIET ON SERUM TNF-ALPHA LEVELS, A MARKER OF LOW-GRADE INFLAMMATION

Cut Indriputri — Mon, 01 Jul 2024 00:00:00 +0700

A high-fat diet is a pattern of excessive fat consumption that can cause various metabolic function disorders, such as hypertension, dyslipidemia, obesity, and increased glucose levels. The aim of this study was to analyze the effect of a high-fat diet on serum tumor necrosis factor alpha (TNF-α) levels as a marker of low-grade inflammation in Wistar rats. The study was designed using a true experimental randomized posttest-only control group. Sixteen male Wistar rats weighing 150-250g and aged 4-5 months were divided into two groups. Group K1 was a negative control group that was given normal diet, while group K2 was a group that was given a high-fat diet, with a fat content of 66.28%. This treatment was controlled for 50 days. The mean TNF-α levels in K1 (290.912 ± 1.87) pg/mL and K2 (295.149 ± 2.76) pg/mL. Based on the results of independent T-test analysis, TNF-α levels in groups K1 and K2 were significantly different (P = 0.034 or P < 0.05). This means that a high-fat diet increases serum levels of TNF-a, a marker of low-grade inflammation.

SKELETON ANALYSIS OF Tursiops truncatus REGIO COSTAE AND SCAPULA STRANDED ON SENGGIGI BEACH, WEST LOMBOK

Mon, 01 Jul 2024 00:00:00 +0700

The dolphin (Tursiops truncatus) is the most studied and most common species of cetacean in captivity. In this study, the size results obtained were the ribs and scapula. The first measure is a difference from the proximal to the distal end between the literature and this study. the second and third sizes have long, short, and longer proximal to distal differences. The fourth, fifth and sixth sizes show that the bones of T.truncatus are smaller than those of the Numataphocoena yamashitai species and the seventh size shows that the differences between T.truncatus and the Numataphocoena yamashitai species are several short and long costae. For the scapula, the dimensions of the first and second bones are larger than the present study species T.truncatus and Numataphocoena yamashitai. The sizes of the three results from this study are almost the same as the species Numataphocoena yamashitai except for the species Prosqualodon marplesi, which is larger than this study and research in California. The fourth size, which is 49.0 mm from head to tuberculum, is larger than the species T.truncatus and Numataphocoena yamashitai studied. The fifth size, the diameter of the rib head of this research species is smaller than the species Numataphocoena yamashitai and Prosqualodon marplesi, both of which are smaller than T.truncatus, and some of these differences are caused by food, habitat and environmental conditions.

NURSING INTERVENTION GIVING WARM COMPRESS TO LARGE VEINS AREAS WITH ABDOMEN WALLS TO DECREASE TEMPERATURE OF HYPERTHERMIC PATIENTS

Risanti Handayani, Hariyono, Arief Hargono — Mon, 01 Jul 2024 00:00:00 +0700

Intra-abdominal organs are receptors that are more sensitive to temperature, cold. While the large vein area is effective because of the vasodilation process by giving warm compresses to reduce body temperature on the body surface. The purpose of this study was to determine the effectiveness of giving warm compresses to the abdominal wall (abdomen) and large vein (axilla) area to decrease the body temperature in fever patients. The design of this research is Quast-Experiment, Design with Nonequivalent Control Group Design approach. The population of this study was all patients with fever as many as 26 respondents. Samples were taken by consecutive sampling technique as many as 20 patients. The research variable is a warm compress in the axilla and abdomen and the independent variable is a decrease in body temperature. Data were collected by observing body temperature and analyzed by using the T-test. The results showed that abdominal compresses were more effective because the number of patients who did not experience a decrease was 1 respondent, while the axilla compression had 2 respondents. The results of the normality test of the data showed that the data were not normally distributed so that the hypothesis testing used the T-test and the results showed that the data was p = 0.000, = 0.05 so that p < a means Ho is rejected, meaning that the difference in body temperature decrease between giving axillary compresses and abdominal compresses is not too significant. and not that much different.

Keywords: Warm Compress, Fever, Body Temperature.

DIFFERENCES IN INTERFERON GAMMA LEVELS IN TREATMENT OF TUBERCULOSIS IN INTENSIVE PHASE AND ADVANCED PHASE

Mon, 01 Jul 2024 00:00:00 +0700

Tuberculosis (TB) is a chronic infectious disease caused by Mycobacterium tuberculosis complex (MTBC). Anti-tuberculosis drugs given to active TB sufferers consist of 3 or 4 combinations. Tuberculosis treatment is divided into intensive phase treatment (2 weeks) and continuation phase (16 weeks / 4 months). Interferon gamma (IFN Î³) is a protein belonging to the cytokine family which plays a role in eliminating MTB bacteria through a cell-mediated immunity mechanism. The aim of this study was to analyze differences in gamma interferon levels in the intensive phase and advanced phase of tuberculosis treatment. This research method is an analytical observational study with a cross-sectional design (cross sectional study). The research design used was a randomized post test only control group design. Data were analyzed using the Kruskal Wallis test with statistical test results obtained with a p value of 0.033 (> 0.05), meaning there was a difference in IFN Î³ levels in the intensive phase and advanced phase of tuberculosis treatment.

DNA TOUCH STR CODIS AS LEGITIMATE EVIDENCE IN UNCOVERING CRIMINAL ACTS

Mon, 01 Jul 2024 00:00:00 +0700

The identification process at the crime scene (TKP) is one of the essential tasks carried out by police investigators. One critical aspect is the collection and analysis of evidence found at the crime scene, referred to as biological evidence (BB). This evidence serves as a crucial source for determining whether an incident occurred and, if so, whether it was a suicide or another criminal act (such as murder, etc.). The biological evidence found can be analyzed using molecular serobiology approaches, which are rapidly advancing in the fields of forensic medicine and medicolegal practice. One such approach involves utilizing DNA traces, although a common challenge is that often the DNA recovered at crime scenes is degraded. Therefore, a DNA amplification method utilizing the Polymerase Chain Reaction (PCR) technique, along with the short tandem repeat (STR) – CODIS identification approach, is necessary. The analysis of DNA traces using the STR-CODIS approach enables investigators to determine whether the incident was purely criminal, a suicide, or another type of accident involving the victim. Furthermore, the DNA analysis using the STR-CODIS method can also aid in identifying the victims discovered at the crime scene.