Blood Description, Parasite Investation and Survival Rate Of Carp (Cyprinus carpio) Which Is Exposed By Spore Protein Myxobolus koi On Rearing Pond as Immunostimulan Material
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ABSTRACT
This research is an experimental research to determine the effect of spore protein Myxobolus koi on grass carp (Cyprinus carpio) in rearing pond. The sample used is grass carp (Cyprinus carpio) 90 fish (10-15 cm). Spore protein used in this research is Myxobolus koi spore protein, that has been found by predecessor researchers and has been tested in laboratory as immunostimulan. The exposure was done orally, mixed in the feed, with a dose of 2 μg / gram dose of protein, feeded 1 time before being reared for 30 days. Parameters measured were: (1) leucocytes description (differentiated leucocytes of carp, (2) Parasitic infestation in carp and (3) Survival Rate / SR of carp were reared on pond for 30 days. The collected data is analyzed descriptively.
The results showed that there was alteration of leukocytes description (differential leukocytes) in carp (Cyprinus carpio) as an indicator of the immune response. A leukocyte differential examination showed that exposure to the Myxobolus koi spore protein, The highest total lymphocytes occurred in carp exposed to Myxobolus koi spores protein and reared from pond in Mojokerto, were 77.6%, Monocytes of 16.3%, Heterophyll 14.4%, Eosinophils 7.6% and Basofil 0.4%. The highest infestation of Myxobolus koi occurred in fish that was not exposed to spore protein was 53.33% after 30 days of reared at pond, while parasitic infestation in fish exposed to spore protein was only 16.66%. Survival rate of carp indicated that the highest occurred in carp that exposed with spore protein and reared from pond in Mojokerto, equal to 90%.
Myxobolus koi spore protein exposure given orally can makes alteration of leukocytes description (differential leukocytes), Decreased parasite infestation and increased survival of carp fish reared for 30 days, with the result that Myxobolus koi spore protein can be developed as an immunostimulant material.
Keyword: Myxobolus koi, Cyprinus carpio, spore protein, immunostimulant, infestation
DAFTAR PUSTAKA
Alejo, A and Tafalla, C., 2011. Chemokines in teleost fish species. Dev. Comp. Immunol. 35: 1215 - 1222.
Amos WB, Routledge LM, and Yew FF, 1975. Calcium-binding proteins in a vorticellid contractile organelle. Cell Sci J, 19 (1) : 203-213.
Anugrahi T, 2013. Efektifitas Daun Ketepeng Cina untuk Penanggulangan Myxobolisis pada Ikan Mas (Cyprinus carpi Linn), Fakultas Perikanan dan Kelautan, Universitas Airlangga. Surabaya.
Anshary, H. 2008. Modul Pembelajaran Berbasis Student Center Learning (SCL) Mata Kuliah Parasitologi Ikan. Jurusan Perikanan Fakultas Perikanan dan Ilmu Kelautan Universitas Hassanudin. Makasar. Hal 126.
Irianto, A. 2005. Patologi Ikan Teleostei. Gajah Mada University Press, Yogyakarta. hal 243.
Ariaty L. 1991. Morfologi Darah Ikan Mas (Cyprinus carpio Linn) Nila Merah (Orechromis sp) dan Lele Dumbo (Clarias gariepinus) dari Sukabumi. [skripsi]. Bogor. Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor.
Badan Karantina Ikan, Pengendalian Mutu dan Keamanan Hasil Perikanan. 2011. Pedoman Penetapan Hama dan Penyakit Ikan Karantina. Kementrian Kelautan dan Perikanan. Jakarta. Hal 25 - 27.
Baratawidjaja. 1996. Immunologi Dasar. Fakultas Kedokteran Universitas Indonesia. Gaya Baru. Jakarta. Hal 12.
Brunk, C.F., 1999. Ciliates display promise for foreign gene expression, Nature AmericaInc,*http://biotech.nature.com.
Clark, T.G., Tian-Long Lin and H.W. Dickerson, 1996. Surface antigen cross-linking triggers forced exit of a protozoan parasite from its host, Proc. Natl. Acad. Sci. USA, Vol. 93, pp.6825 – 6829, June.
Devi, H. L. N. A. 2011. Hubungan Derajat Infestasi Myxobolus koi Terhadap Jumlah Spora Dan Derajat Kerusakan Pada Usus Ikan Mas (Cyprinus carpio L). Skripsi. Budidaya Perairan. Fakultas Perikanan dan Kelautan. Universitas Airlangga. Surabaya. Hal 60.
Dwijayanti, A. A. 2011. Karakterisasi protein nodul pada insang ikan mas (Cyprinus carpio) akibat infestasi Myxobolus sp dengan metode SDS-PAGE. Skripsi. Fakultas Perikanan dan Kelautan Universitas Airlangga. Hal 2.
Easy R. H., S. C. Johnson and D. K. Cone. 2005. Morphological and molecular comparison of Myxobolus procerus (Kudo, 1934) and M. intramusculi n. sp. (Myxozoa) parasitizing muscles of the trout-perch Percopsis omiscomaycus. J.Parasitol 61:115–122.
El-Matbouli. M., T. W. Holstein and R. W. Hoffmann. 1998. Determination of nuclear DNA concentration in cells of Myxobolus cerebralis and triactinomyxon spores, the causative agent of whirling disease. J.Parasitol 84: 696-699.
Eszterbauer E., M. Benko, A. Dan and Molnar K. 2001. Identification of fish parasitic Myxobolus (Myxosporea) species using a combined PCR-RFLP method. J.Dis Aquat Org 44:35–39.
Farmer, J. N. 1980. The Protozoan Introduction to Protozoology. The C.V. Mosby Company, St.Louis. 35-36 p.
Firmansyah, R. A. F. 2012. Prevalensi Dan Jumlah Nodul Pada Insang Ikan Koi (Cyprinus Carpio) Yang Terinfeksi Myxobolus Di Sentra Budidaya Ikan Koi Kabupaten Blitar-Jawa Timur. Skripsi. Budidaya Perairan. Fakultas Perikanan dan Kelautan. Universitas Airlangga. Surabaya. Hal 42-43.
Guyton Arthur C. and Hall John E. 1999. Buku Ajar Fisiologi Kedokteran. Setiawan I, Tengadi KA, Santoso A, penerjemah: Setiawan I, editor. Jakarta : EGC. Terjemahan dari : Textbook of Medical Physiology. Hal 25-27.
Itabashi T, Mikami K and Asai H, 2003. Characterization of the spasmin I gene in Zoothamnium arbuscula strain Kawagoe (protozoa, eiliophora) and its relation to other spasmins and centris. Res. Microbiol J, 154 ( 5 ) : 361 - 367.
Kudo, R. R., 1977. Protozoology. Fifth Edition. Publisher Ilinois. USA. p.125-131.
Kementerian Kelautan dan Perikanan, 2010. Peikanan Budidaya. Jakarta. Hal 14-16.
Leff, A.A., T. Yoshinaga and H.W. Dickerson, 1994. Cross immunity in channel catfish, Ictalurus punctatus (Rafinesque), against two immobilization serotypes of Ichthyopthirus multifiliis (Fouquet), J. of Fish Diseases, Vol. 17, pp. 429 – 432.
Levine ND. 1994. Buku Pelajaran Parasitologi Veteriner. Ashadi, penerjemah. UGM Gadjah Mada Uni Press. Yogyakarta. Terjemahan dari: Textbook of Veterinary Parasitology. Hal 31-32.
Lin, T.L., T.G. Clark and H. Dickerson, 1996. Passive Immunization of Channel Catfish (Ictalurus punctatus) against the Ciliated Protozoan Parasite Ichthyophthirius multifiliis by Use of Murine Monoclonal Antibodies, Infection and Immunity, Oct, pp. 4085 – 4090.
Lom, J and I. Dykova. 1992. Protozoan Parasites of Fishes. Developments in Aquaculture and Fisheries Science. 26: 315 p.
Lom, J and I. Dykova. 2006. Myxozoan genera: Definition and notes on taxonomy, life cycle, terminology and pathogenic species. J.Folia Parasitol. 53: 35-36.
Mahasri, G. 2007. Buku Ajar Ilmu Penyakit Protozoa Pada Ikan dan Udang. Universitas Airlangga. Surabaya.
Maki, J.L. and H.W. Dickerson, 2003. Systemic and Cutaneous Mucus Antibody Responses of Channel Catfish Immnunized against the Protozoan Parasite Ichthyophthirius multifiliis, Clinical and Diagnostic Laboratory Immunology, Vol. 10, No. 5, pp.876 – 881.
Maswan, N. A. Pengujian Efektivitas Dosis Vaksin Dna dan Korelasinya Terhadap Parameter Hematologi Secara Kuantitatif. 2009. Skripsi. Teknologi dan Manajemen Akuakultur. Fakultas Perikanan dan Ilmu kelautan. Institut Pertanian. Bogor. Hal 33.
Mcutcheon SM, NB Mclaughhlin and HE Buhse Jr, 2002. Characterization of Cytoskeletal, Calcium-Binding Proteins in Vorticella convallaria, Eukaryot Microbiol J.
Moriyama Y, H Okamoto and H Asai, 1999. Rubber-Like Elasticity and Volume Changes in the Isolated Spasmoneme of Giant Zoothamnium sp. under Ca2+-Induced Contraction, Biophys J, 76 (2) : 993-1000.
Nazir, M. 2011. Metode Penelitian. Ghalia Indonesia. Bogor. hal. 63-64,124.
Preer, J.R, Jr., 1986. In the molekcular biology of ciliated protozoa, ed. Gall, J.G (Akademic London). Pp. 301-339.
Pylawka, S and Buhse, Jr HE, 2003. Protein Synthesis and Telotroch Formation in Vorticella convallaria, Eukaryot Microbiol J, 198 : 45 - 52
Randelli, E., F. Buonocore and G. Scapigliati. 2008. Cell markers and determinants in fish immunology. J.Fish Shell Immuno. 25: 326-340.
Robert, J. R. 1989. Fish Pathology Second Edition. Bailliere Tindall. London. 64 p.
Routledge LM, 1978. Calcium-binding proteins in the Vorticellid spasmoneme : Extraction and Characterization by Gel Electrophoresis,, Cell Biology J, 77 : 358-370.
Randelli, E., Buonocore. F, and Scapigliati. G., 2008. Cell markers and determinants in fish immunology. J. Fish & Shellfish Immunology, 25:326-340.
Sommerset I, Krossí¸y B, Biering E, Frost P. 2005. Vaccines for fish in aquaculture. Exp Rev Vac. 4: 89-101.
Steel R. G and Torrie J. H. 1993. Prinsip Prosedur Statistika, Terjemahan oleh Bambang Sumantri. Gramedia. Jakarta. Hal 425-478.
Stoskopf, M. K. 1993. Fish Medicine. Clinical Pathology of Carp, Goldfish and Koi. Mexico. 882: 54-56.
Stoskopf, M. K and Michael, W.B. 1992. Fish Medicine. Saunders Company, Philadelphia, PA. 11 p.
NACA. 1991. Fish Health Management. In : Asia Pacific report on a Regional Study and Workshop on Fish Diseases and Fish Health Management, ADB Agriculture Department Report Series No.1 Network of Aquaculture Center in Asia-Pacific, Bangkok. 627 pp.
Sugianti, B., R. C. Tarumingkeng, Z. Coto dan Hardjanto. 2005. Pemanfaatan Tumbuhan Obat Tradisional Dalam Pengendalian Penyakit Ikan. Makalah Pribadi Falsafah Sains (PPS-702). Program Pasca Sarjana. Institut Pertanian Bogor. Hal 7-8.
Titis, C. D., W. S. D. Nugroho, D. Dudung, H. Nurul dan Sumayani. 2009. Laporan Uji Coba Identifikasi dan Penentuan Derajat Kerusakan akibat Infeksi Myxobolus koi pada Ikan Mas. Balai Karantina Ikan Kelas II. Semarang. Hal 66.
Tizard I. 1988. Pengantar Imunologi Veteriner. Ed ke-2. Partodirejo M, Hardjosworo S, penerjemah; Surabaya: Airlangga University Press. Terjemahan dari: An Introduction to Veterinary Immunology.
Wijanarko, P. Y. 2011. Hubungan Jumlah Spora Terhadap Jumlah dan Ukuran Nodul Myxobolus koi Yang Menyerang Ikan Mas. Skripsi. Budidaya Perairan. Fakultas Perikanan dan Kelautan. Universitas Airlangga. Surabaya. Hal 15.
Wang, Xuting and H.W. Dickerson. 2002. Surface Immobilization Antigen of the Parasitic Ciliate Ichthyophthirius multifiliis Elicits Protective Immunity in Channel Catfish (Ictalurus punctatus), Clinical and Diagnostic Laboratory Immunology, January, Vol. 9, No. 1, p. 178 -181, Athens, Georgia.
Woro, A.R., Gunanti, M. dan Lucia, T.S., 2015. Efektivitas Vaksinasi Crude dan Soluble Protein Spora Myxobolus koi terhadap tingkat kerusakan usus ikan koi (cyprinus carpio koi). Jurnal Ilmu Perikanan dan Kelautan. April, Vol 2. No.2. Hal. 12-17
Yuasa, K., Panigoro, N., Bahnan, M., dan Khiidin., B. E. 2003. Panduan Diagnostik Penyakit Ikan. Teknik Diagnosa Penyakit Ikan Budidaya Air Tawar. Balai Budidaya Tawar Jambi.
Yusuf, M. 2015. Analisis Respons Imun Ikan Koi (cyprinus carpio koi) Yang Divaksin Dengan Whole Protein Spora Myxobolus koi Sebagai Kandidat Vaksin Myxobolusis. Tesis. Sekolah Pascasarjana, Universitas Airlangga. Surabaya. 64 Halaman.
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