Journal of Aquaculture and Fish Health

Effect of vitamin C supplementation on the survival rate and histopathological changes of gills and kidneys of tilapia (Oreochromis niloticus) infected by Aeromonas hydrophylla

2023-03-31T12:04:00+00:00

This study was conducted to determine the effect of vitamin C supplementation on the survival rate and histopathological changes of gills and kidneys of tilapia infected by Aeromonas hydrophila. Three doses of vitamin C were tested (150; 300; and 450 mg/kg) with two control groups. Tilapia with uniform size (average weight of 14 grams) as the criteria for inclusion were randomly distributed in five ponds with 15 tilapia fish per ponds. Tilapia was fed with hands until full for two weeks. Tilapia was then infected with A. hydrophila to find out the survival rate and histopathological changes of gills and kidneys at the end of the experimental period or 7 days after infection. The supplementation of 150 mg/kg vitamin C in feed increased the survival rate (%) in Tilapia by 86.67% or higher than other treatment and control groups. The damage to gills in terms of lamella separation was found in all treatment groups, as well as kidneys. The results found that the supplementation of 150 mg/kg vitamin C in feed increased survival rate but did not give effective protection on gills and kidneys.

Spatial Distribution Of Heavy Metals Cd And Cu In Water, Sediment And Fish (Mugil Sp) In Pelawangan East Segara Anakan Cilacap

2023-10-02T14:20:30+00:00

Heavy metals are widely known pollutants with bioaccumulative nature, so they can be transferred and accumulated in biota, such as mullet fish (Planiliza subviridis). The heavy metals Cd and Cu have different anthropogenic and natural sources. Heavy metals have the potential to absorb existing biota such mullet fish. Fish absorb metals from the water in the body through gills, skin surface and food. This study aims to identify Cd and Cu metals in distribute water, sediment and mullet fish in the Plawangan Timur, Segara Anakan lagoon, Cilacap, Central Java, and the relationship between aquatic media and sediment and mullet fish. The study used a research method with a targeted random sampling method at five stations with four replications. The survey was conducted from April to September 2020. Descriptive data analysis, F-test, regression and correlation. The results showed in water, sediment and mullet fish the heavy metal content of Cd and Cu varied between stations. The correlation between the Cd and Cu metals in water, sediment and mullet fish showed a positive correlation. The content of heavy metal Cd in mullet fish media in Plawangan Timur Segara Anakan Cilacap has exceeded the specified quality standard, while for heavy metal Cu in mullet fish is still below the specified quality standard.

Relationship Between The Dynamics of Plankton Community Abundance, Total Organic Matter, and Salinity in Intensive Shrimp Farming Systems

2024-01-02T16:29:57+00:00

Plankton plays an important role in shrimp cultivation in ponds, both as an indicator of water quality and as a natural food source for shrimp that are raised. The purpose of this study was to measure the relationship between the dynamics of the plankton community, the concentration of total organic matter, and water salinity with the abundance of plankton in intensive shrimp farming systems. The study was conducted on six units of ponds located in Cirebon district, West Java, Indonesia. The ponds were lined with HDPE on each side and plastic mulch at the bottom. Post larvae of shrimp were stocked at an average density of 110 shrimp.m^-2 and maintained for 98 days. Water quality samples were collected for daily measurement. Data were analyzed descriptively and then analyzed using Pearson's correlation test and regression. The results of this study showed that during the shrimp rearing period, 65 species of plankton were identified, consisting of Chlorophyta 20%, Cyanophyta 15.38%, Diatoms 26.15%, Euglenophyta 6.15%, Dinoflagellates 4.62%, Protozoa 10.77%, and Ciliates 16.92% with an average density ranging from 7.56x10⁵-19.99x10⁵ cells.mL^-1. Chlorella sp. (Chlorophyta) and Oscillatoria sp. (Cyanophyta) were found to be the dominating species in all ponds. Total phytoplankton density tended to be higher during the first 54 days of culture. The results of the correlation and regression tests showed that the composition of plankton, the concentration of total organic matter, and the salinity of the water affected the abundance of plankton in the pond.

Effect of Giving Api-Api (Avicennia marina ) Mangrove Leaf Solution In Artificial Feed On The Immunity Response and Growth of Vannamei Shrimp (Litopenaeus vannamei)

2024-03-11T02:39:41+00:00

The purpose of this study was .to determine the effect of giving Avicennia marina mangrove leaf solution on artificial feed to increase the immune response and growth of vannamei shrimp. The parameters observed in this study were the enhancement of PA value (Phagocytosis Activity), ADG (Average Daily Growth), FCR (Feed Conversion Ratio), and EPP (Efficiency of Feed Utilization). The vannamei shrimp used was PL-20 fry with the weight of 1,09 – 1,13 gâ‹…head^-1and density 1 shrimp L^-1. The method used in this study was an experimental method in the laboratory, with a completely randomized design (CRD) with 4 treatments and 3 replications. This research was conducted by adding Avicennia marina mangrove leaf solution to the feed, with treatments of A (control), B (125 "°), C (175 "°), and D (225 "°). The addition of Avicennia marina leaf solution to the feed had a significant effect on the increase of phagocytosis activity and growth of the shrimp with F count of 30,773 which was higher than the F table of 4,06. Moreover, the best dose obtained in treatment D which resulted in an increase in PA was 61,9%, ADG was 0,22 gâ‹…day^-1, FCR was 1,29, and EPP was 79,04%.

Optimizing Tilapia (Oreochromis niloticus) Growth through a Comparative Evaluation of EM4 and Homemade Probiotics in Pellets

2024-02-21T17:52:27+00:00

One alternative approach to facilitate the utilization of feed by tilapia is the addition of probiotic in feed. In this study, we compare the effects of commercial probiotic EM4 and homemade probiotics on tilapia growth. This research was conducted in PT. Sahaja Berkah Abadi at wonosalam, jawatimur, indonesia. The study was designed as a completely randomized design with 3 treatments and 4 replications, encompassing control group (C), commercial probiotics (G1), and homemade probiotic (G2). The commercial probiotics (G1) contained Rhodopseudomonas sp., Lactobacillus sp., Streptomyces sp., and Actinomycetes sp. while the homemade probiotic (G2) contained starter bacteria Lactobacillus casei and herbal plant such as ginger, curcuma, and tumeric. Parameters observed during the study included weight gain (WG), specific growth rate (SGR), and survival rate (SR). The results revealed that both G1 and G2 performed better than the control group. The weight gain for the control, G1, and G2 groups were 2.38±0.03 g, 3.09± 0.01 g, and 3.13 ± 0.03 g, respectively. Similarly, the specific growth rates for the control, G1, and G2 groups were 4.25±0.03%, 5.52± 0.02%, and 5.59 ± 0.03%, respectively, while the survival rate remained at 100% across all groups. The water quality in all treatments during maintenance met the standard requirements for tilapia, with temperatures ranging from 25.5 to 26.7 °C and pH levels between 6.7 and 6.9. These findings suggest that the probiotics incorporated in G2 present a cost-effective alternative to those in G1, offering a promising option for tilapia cultivation.

Utilization of Cow's Rumen Bokashi Enriched with Chicken Manure on Chlorella sp. Cell Density

2024-01-26T15:36:26+00:00

One of the organic fertilizers that has the potential to cultivate Chlorella sp. is the cow's rumen. To increase the nutrient content in the cow's rumen, the cow's rumen must be made into bokashi and enriched with chicken manure. This study aimed to determine the use of cow's rumen bokashi enriched with chicken manure at different doses on Chlorella sp cell density. The research design used was a Completely Randomized Design (CRD) with 5 treatments and 3 replications, namely P0 (2 g/L cow's rumen bokashi), P1 = 2 g/L cow's rumen bokashi enriched with 2.5 g/L chicken manure, P2 = 2 g/L cow's rumen bokashi enriched with 3.0 g/L chicken manure, P3 = 2 g/L cow's rumen bokashi enriched with 3.5 g/L chicken manure, and P4 = 2 g/L cow's rumen bokashi enriched with 4.0 g/L chicken manure. The results showed that the highest cell density of Chlorella sp. occurred in treatment P3 (i.e. 456.1 ± 15.1 í— 10⁴ cells/mL) and the highest specific growth rate occurred in treatment P0 (i.e. 0.20 ± 0.0/day). Nutrient levels in the culture medium are optimal for Chlorella sp., with nitrate ranging from 0.1375 to 0.2833 mg/L and phosphate ranging from 2.4889 to 2.8650 mg/L. From the results of this study, it can be concluded that the use of cow's rumen bokashi enriched with chicken manure had a very significant effect on cell density and specific growth rate of Chlorella sp. (P<0.01).

Effect of two aquatic ambient factors (pH and Dissolved Oxygen) on antioxidant generation in skeletal muscle of zebrafish (Danio rerio, Hamilton 1822)

2023-12-05T14:38:52+00:00

Ambient factors in aquatic body are often responsible for oxidative stress in fish. Zebrafish has been frequently targeted for study to understand such hypoxic effects. In this study, two ambient factors, viz. DO saturation (20-30%, 40-50%, 60-70% and, 80% and above) and pH (4.5-5.5, 5.5-6.5, 6.5-7.5, 7.5-8.5 and 8.5-9.5) were experimented for generation of antioxidant (Catalase, SOD and Glutathione) in the skeletal muscle of zebrafish. The oxidative stress was marked with the levels of MDA in the skeletal muscle. These conditions were tested against the length of treatment period (in hr) from 4hr to 16 hr for each DO saturation level and 1hr to 4 hr for each pH level. The analysis of data shows that, for DO saturation, the skeletal muscle of zebrafish suffered maximum at 12 hr of treatment period having highest level of MDA against 20-30% of saturation level. Similarly, for pH, this period was 2hr and the treatment level was pH 4.5-5.5. Although alkaline ambiance (pH 8.5-9.5) also exerted strong oxidative stress at 2 hr, but it was significantly low in terms of generation of MDA. In all cases, the antioxidant levels spiked after post maximum generation period i.e. 16 hr for DO saturation and 2 hr for pH level. This indicates a time point wherefrom the fish undergoes oxidative stress and initiation of a counter mechanism during the post oxidative stress period in these two ambient conditions. A two-way ANOVA has explained that the effect of treatment period and the level of ambient factors significantly contributed to the antioxidant generation in zebrafish. The outcome of this work will directly help in management of fish culture when the aquatic body turns to hypoxic or acidic in nature.

Analysis Of Mercury (Hg) In Mujair Fish (Oreochromis mosambicus) In Limboto Lake, Gorontalo Province

2021-03-08T05:25:08+00:00

The occurrence of silting of the lake and sedimentation in Limboto Lake has become a place for the deposition of heavy metals, especially mercury. In recent years it has been known that the water of Lake Limboto has been polluted by mercury based on data from the Agency for the Environment, Research and Technology (Balihristi), Gorontalo Province in 2007.This study aims to analyze the mercury (Hg) content of mujair fish (Oreochromis mossambicus) in Limboto Lake. This research was conducted in November 2018- June 2019. The method used in this research is descriptive method. Sampling of water and fish was carried out by purposive sampling by dividing the research location into 2 stations, namely Station I in the floating net cages and Station II outside the KJA Measurement of mercury content (Hg) was carried out at the Laboratory of Fisheries Product Quality Development and Testing (LPPMHP) Gorontalo City using the Atomic Absorbtin Spectrophotometer (AAS). From the results of the study, it was found that, the Mercury (Hg) Content in Lake Limboto Waters, Stasion I was 0.0014 ppm and Station II was 0.0007 ppm, and for mujair fish organs the highest was in meat, namely outside KJA 0.0037 ppm and in meat, the lowest was in KJA 0.0035 ppm.. Nakoe et al. (2014) examined the risk of exposure to mercury (Hg) in humans who consume Mujair (Oreochromis niloticus) from Lake Limboto using the Limboto approach. Limboto using an environmental health analysis approach with a variable test method of body weight, consumption level and frequency. Concentration mercury levels of tilapia in Lake Limboto are between 0.007 - 0.089 mg/kg while the mercury levels in the lake are between 0.0014 mg/L. lake is around 0.0014 mg/L.

Effect of Additional Dried Tubifex sp. in Commercial Feed Against Color Intensity of Guppy (Poecilia reticulata)

2022-09-22T12:10:56+00:00

Guppy fish (Poecilia reticulata) is a type of ornamental fish that is in great demand, because of its small size and beautiful color. The color of ornamental fish will generally fade, due to a lack of carotenoids in their feed. The purpose of this study was to determine the effect of giving Tubifex sp. in commercial feed to increase the color intensity of guppy fish and the best dose of Tubifex sp. The test fish used were male guppy fish strain HB Red. This study used a completely randomized design (CRD) method with five treatments and three replications, that is commercial feed with the addition of Tubifex sp. with doses of 0% (P1), 2% (P2), 4% (P3), 6% (P4), and 8% (P5). Parameters observed were color intensity (chroma value), survival, and water quality. Addition of Tubifex sp. in commercial feed gave the effect with the highest yield on P5 at a dose of 8%, with an increase in color intensity (chroma value) of 4,21±0,25^d. At P1 it gave an increase of 1,19±0,02^a, P2 was 1,34±0,04^a, P3 was 1,81±0,21^b, and P4 was 2,88±0,18^c. So, the best treatment is P5 (8%). Survival showed the results were not significantly different, that is 100%. Water quality is included in the tolerance limit of fish with the results of temperature 26,6 – 27,4°C, pH 7,4 – 7,9, and DO 6,2 – 7,7 mg/L.

Initial Development of Lamota (Salicornia europae) Yogurt As a Functional Drink

2024-02-01T15:18:53+00:00

Salicornia europae is a marine plant that grows in coastal areas and tides. In Sumbawa, this plant lives in the former area of ponds and usually called as lamota. S. europae contains beneficial ingredients such as iodine, minerals, vitamins, antioxidants, unsaturated fatty acids, and sugars. Marine plant innovations in S. Europae, which is rich in iodine into processed yogurt, has not been well explored. This study aimed to develop the processing of lamota into functional yogurt drink. Complete Randomized Design (CRD) was used in this experiment with a single factor of 6 levels of lamota concentration. Iodine content, ash content, pH and scoring test were carried out to find out the best quality of the lamota yogurt. The results showed that the iodine content of the product was 29.01 mcg / g dry weight and ash content was 10.59%. The pH showed that the addition of lamota minimally affected the pH of the yoghurt. In the organoleptic test found that 25% addition of lamota extract was the most preferrable concentration compared to 5, 10, 15, and 20% based on the color, taste, texture, aroma. Panelist describe the 25% lamota addition has greenish color, a little bit salty, watery texture, and green aroma. Based on the iodine content and the best consumers acceptance the yogurt with 25% addition of lamota could be categorized as a rich in iodine drink and a promising product to improve the functional characteristics of yogurt as well develop a value added local marine commodity with unique flavor.

The Use of OODEV® in Improving the Reproductive Performance of Male Yellow Rasbora Fish Rasbora lateristriata (Bleeker, 1854)

2023-11-01T13:41:18+00:00

A local commodity, Yellow rasbora fish Rasbora lateristriata (Bleeker, 1854), can potentially be used as a food resource by the Kulon Progo community. Added to that, cultivation or aquaculture is known to optimize this potential. Furthermore, Aquaculture will also prevent illegal fishing in nature, where yellow rasbora fish are listed as vulnerable species in the IUCN Red list. This study induced male yellow rasbora fish using the hormone OODEV^® injection, which comprises the hormone Pregnant Mare Serum Gonadotropin (PMSG) and anti-dopamine. PMSG is a hormone that contains FSH as well as LH in it. Yellow rasbora fish were nurtured to the age of six months and treated with four different treatments (dose) for a month, namely 0 mL/Kg, 0.25 mL/Kg, 0.5 mL/Kg, and 0.75 mL/Kg. Observed parameters included motility (movement and duration) as well as sperm count, sperm viability, sperm morphology, sperm morphometry, gonadosomatic index (GSI), and survival rate (SR). Data obtained were analyzed using SPSS with the One-way ANOVA test and continued with Duncan's test if the results were significant. The obtained results had no significant effect on the IGS value (p>0.05), with the highest value found at a dose of 0.75 mL/Kg. In addition, administration of OODEV^®also improved sperm quality with the best results at 0.75 mL/Kg in terms of motility with the highest progressive movement compared to other doses but not significant (p>0.05) compared to the control, and significantly (p<0.05) longest movement duration compared to other doses, while in terms of morphometry it had the second widest middle piece area but not significant (p>0.05) compared to other doses, widest tail area but not significant (p>0.05) compared to other doses except with 0.25 mL/Kg, longest tail length (p>0.05) but not significant with other doses except with 0.25 mL/Kg , and significantly (p<0.05) had the longest sperm total length. Every dose showed a 100% percentage of survival rate.

Natural Spawning Breeding Techniques Analysis of Carp (Cyprinus carpio) in the Fish Seed Center (BBI) Bolangan, Tabanan, Bali

2023-08-05T03:44:04+00:00

Carp (Cyprinus carpio) is a freshwater fish species with a high tolerance to various environmental conditions. The present study aimed to analyze the seeding process of carp using a natural spawning method at the Fish Seed Center (BBI) Bolangan, Tabanan, Bali. The descriptive analysis had been used to analyze of the data collected through active participation, observation, and interviews. The carp natural spawning techniques at BBI Bolangan were conducted in an earthen pond. The breeding techniques include pond preparation, brood stock selections, fish spawning, larvae or seed maintenance, water quality control, disease control, and fish harvesting. Male and female parents in a 2:1 ratio were required for effective breeding. This natural hatching system produced up to 100806 eggs with a hatching rate of 74.4%, yielding 75000 larvae per female parent. After a week of care, the larvae have a 30% survival rate and are ready for cultivation in the following section (nursery II). We conclude that the Carp (Cyprinus carpio) breeding techniques at the Fish Seed Center (BBI) Bolangan, Tabanan, Bali are technically sound and feasible to apply.

Utilization of Nipa Leaves Meal (Nypa fruticans Wurmb) as a Substitute for Rice Bran of Striped Catfish Feed (Pangasius hypophthalmus)

2024-01-26T14:41:10+00:00

Feed is one of the important components of aquaculture. The higher the feed price, it is necessary to look for alternative materials for feed ingredients. One of the alternative feed ingredients that can be used is rice bran. In certain seasons, rice bran production decreases. One of the ingredients that can be used as a substitute for rice bran is nipa leaves. This study aims to determine the effect of using nipa leaves meal to replace rice bran in feed formulations and to examine the best percentage of nipa leaves meal on the growth and survival of striped catfish. This study used a completely randomized design (CRD) which consisted of 5 treatments (P) with three replications, namely P0 (0% nipa leaves meal: 20% rice bran), P1 (5% nipa leaves meal: 15% rice bran), P2 (10% nipa leaves meal: 10% rice bran), P3 (15% nipa leaves meal: 5% rice bran), and P4 (20% nipa leaves meal: 0% rice bran). The size of fish used in this study was 4±0.5 cm with a sticking density of 1 fish/L. The rearing of the study was 42 days. Research results show that using nipa leaves meal as a substitute for rice bran with different significantly affects the growth of absolute weight, absolute length, feed efficiency, and protein efficiency ratio. Research results show that the P4 treatment with a percentage of 20% nipa leaves meal: 0% rice bran is the best result with absolute weight growth (2.70 g), absolute length growth (3.35 cm), feed efficiency (85.88%), the protein efficiency ratio (2.66%), and survival (100%). Water quality obtained during rearing is temperature 25.2–31.2^oC, pH 6.9–8.7, and dissolved oxygen (DO) 5–6.1 mg/L.

Effect of Papaya Leaf Solution (Carica papaya Linn) on the Hatching Percentage and Survival Rate of Dumbo Catfish Larvae (Clarias sp.)

2023-10-12T00:07:13+00:00

Eggs of dumbo catfish are known to stick to the substrate due to the presence of a mucus layer, which causes suboptimal oxygen supply and low hatching percentage. This indicates that the mucus layer must be lowered while also eliminating the need for a substrate during the hatching process. One of the natural ingredients that can be used for this purpose is papaya leaf containing the proteolytic enzyme papain. Therefore, this study aims to determine the effect of papaya leaf solution on the percentage of live embryos after soaking, hatching percentage, hatching time, survival rate, and abnormality percentage of dumbo catfish larvae. This research was carried out at the Basic Fisheries Laboratory, Aquaculture Study Program, Fisheries Department, Faculty of Agriculture, Sriwijaya University in August-October 2022. A Completely Randomized Design (CRD) was used, which consisted of four treatments and three repetitions. The treatment given was the soaking of eggs in papaya leaf solution with different concentrations of (P0/control), 2 g/0.96 L (P1), 4 g/0.96 L (P2), and 6 g/0.96 L (P3). The results showed that P2 was the best treatment with 100% live embryos after soaking, 94.06% hatching percentage, 20.24 hours hatching time, 92.78% survival rate, and 0.93% abnormality percentage. Based on these results, the soaking of dumbo catfish eggs on papaya leaf solution could increase the hatching percentage and survival rate.