Potential of Andrographis paniculata and Morinda citrifolia as Natural Anticoccidial Agents in Broiler Chickens: An In Vitro Study
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Coccidiosis is a parasitic disease that commonly affects broiler chickens, caused by protozoa of the genus Eimeria. This disease significantly reduces productivity, impairs feed efficiency, and increases mortality rates. The continuous use of synthetic anticoccidial drugs has raised concerns over resistance and drug residues accumulation in poultry products. This study aimed to evaluate and compare the in vitro anticoccidial efficacy of herbal powders from Andrographis paniculata (sambiloto) and Morinda citrifolia (noni leaves) as potential natural alternatives. A total of 150 fecal samples were collected from five poultry farms using a simple random sampling method. Eight treatment groups were established: a negative control (without coccidiostat), a positive control (trimethoprim-sulfamethoxazole), and six herbal treatments (0.25%, 0.5% and 0.75% of both A. paniculata and M. citrifolia powders). Each treatment group comprised five biological replicates. Oocyst per gram (OPG) counts were determined using McMaster technique. Both herbal powders significantly reduced OPG compared to the negative control (p < 0.05). The most effective treatment was M. citrifolia at 0.75%, showing the lowest OPG value and comparable efficacy to the synthetic anticoccidial. M. citrifolia 0.75% reduced mean OPG to 4,098 ± 250 (p < 0.001) compared to the negative control. This study provides the direct comparative evidence of A. paniculata and M. citrifolia anticoccidial activity using standardized OPG quantification, suggesting their promise as sustainable herbal alternatives for coccidiosis control.
Abdel-Tawab, M. 2021. Considerations to Be Taken When Carrying Out Medicinal Plant Research—What We Learn from an Insight into the IC50 Values, Bioavailability and Clinical Efficacy of Exemplary Anti-Inflammatory Herbal Components. Pharmaceuticals, 14.
Amrane-Abider, M., Benidir, M., Benaissa, M.L., Messaoudene, D., Ouchene, N., Ouchene-Khelifi, N.A. and Ziane, L., 2023. Bioactive compounds and in vitro antioxidant and anticoccidial activities of Opuntia ficus-indica flower extracts. Biomedicines, 11(8), pp.1–14.
Arsyitahlia, N., Bagus, I., Ardana, K., Ayu, I. and Apsari, P., 2019. Prevalensi infeksi Eimeria spp. pada ayam pedaging yang diberi pakan tanpa antibiotik growth promoters (AGP) di Kabupaten Tabanan, Bali. (Prevalence of Eimeria spp. Infection in Broiler Chickens Fed Antibiotic-Growth-Promoter-Free Diets in Tabanan Regency, Bali). Indonesia Medicus Veterinus, 8(2), pp. 186-192.
Asare, C., Aziato, L., & Boamah, D. 2021. Facilitators and barriers to the clinical administration of herbal medicine in ghana: a qualitative study. BMC Complementary Medicine and Therapies, 21(1).
Bushkin, G.G., Motari, E., Carpentieri, A., Dubey, J.P., Costello, C.E., Robbins, P.W. and Samuelson, J., 2013. Evidence for a structural role for acid-fast lipids in oocyst walls of Cryptosporidium, Toxoplasma, and Eimeria. mBio, 4(5), pp.1–8.
Cahyaningsih, U., Riandci, R. and Iswantini, D., 2012. Ekstrak sambiloto (Andrographis paniculata) menurunkan jumlah skizon, mikrogamet, makrogamet, dan oosista Eimeria tenella (Andrographis paniculata Extract Reduces the Number of Schizonts, Microgamonts, Macrogamonts, and Oocysts of Eimeria tenella). Jurnal Veteriner, 13(3), pp.322–329.
Chaves de Jesus, P., Rego Rodrigues Silva, D. M., Macedo Moura, P. H., Gopalsamy, R. G., Dias Silva, E. E., dos Santos Barreto, M., Santana Santos, R., Santos Martins, A. Y., de Freitas Almeida, A. G., Santana Corrêa, A. K., Alves da Mota Santana, L., Hariharan, G., Gibara Guimarães, A., & Pinto Borges, L. 2025 The In Vitro Pharmacokinetics of Medicinal Plants: A Review. Pharmaceuticals, 18(4), 551.
Choi, J. and Kim, W.K. 2020. Dietary application of tannins as a potential mitigation strategy for current challenges in poultry production: A review. Animals 10(12), pp. 1–21.
Correia, S., Suratma, N.A. and Oka, I.B.M., 2021. Prevalensi dan intensitas infeksi Eimeria spp. April–Mei 2021 pada ayam petelur lebih tinggi daripada ayam pedaging di Tembuku, Bangli, Bali (Prevalence and Intensity of Eimeria spp. Infection in Laying Hens Are Higher Than in Broilers During April–May 2021 in Tembuku, Bangli, Bali). Indonesia Medicus Veterinus, 11(3), pp.343–349.
Dewi, N. P. J. R., Dwinata, I. M., Kardena, I. M., Suarjana, I. K., and Kencana, G. A. Y. 2025. Case of coccidiosis in broiler chickens at a farm in Benoa Village, Badung Regency, Bali. Buletin Veteriner Udayana, 17(1), pp.151–162.
Ekawasti, F. and Martindah, E., 2019. Control of coccidiosis in chickens through herbal medicine. WARTAZOA, 29(1), pp.1–12.
El-Ghareeb, W. R., Kishawy, A. T. Y., Anter, R. G. A., Aboelabbas Gouda, A., Abdelaziz, W. S., Alhawas, B., Meligy, A. M. A., Abdel-Raheem, S. M., Ismail, H., and Ibrahim, D. 2023. Novel Antioxidant Insights of Myricetin on the Performance of Broiler Chickens and Alleviating Experimental Infection with Eimeria spp.: Crosstalk between Oxidative Stress and Inflammation. Antioxidants, 12(5), pp.1–19.
Fitri, D.Y., Agustin, A.L.D., Kholik, K. and Janah, M. 2021. Deteksi Koksidiosis pada Ayam Layer di Sesaot, Kecamatan Narmada, Kabupaten Lombok Barat. Media Kedokteran Hewan 32(3), pp. 97–104.
Indrati, R. and Titisari, N., 2020. The effectiveness of phytopharmaca of ethanol extract sambiloto leaf as an alternative of control to coccidiosis. In: IOP Conference Series: Earth and Environmental Science. Bristol: Institute of Physics Publishing.
Irianti, I.N., Suwanti, L.H., Setiawan, B., Ramadani, D., Prabowo, M.A. and Widiastuti, Y., 2024. Efektifitas ekstrak sambiloto penyarian air terhadap isolat Eimeria maxima secara in vitro (Efficacy of Aqueous Andrographis paniculata Extract Against an Eimeria maxima Isolate In Vitro). Jurnal Ilmiah Veteriner Yogyakarta, 5(1), pp.57–67.
Irianti, I.N., Wijayanti, A.D. and Mulyani, G.T., 2022. The anticoccidial property of sambiloto (Andrographis paniculata) leaf extract. Indonesian Journal of Veterinary Sciences, 3(1), pp.8–13.
Karimy, M., Julendra, H., Sofyan, A., Damayanti, E. and Priyowidodo, D., 2013. Effectiveness of Cosmos caudatus Leaf, Morinda citrifolia Leaf, and Lumbricus rubellus Powder in Water-Soluble Granules as Natural Coccidiostat Against Eimeria tenella Infection in Broiler Chickens. Jurnal Ilmu Ternak Veteriner, 18(2), pp.88–98.
Li, J.Y., Wang, S.H., Zhu, D., Zhang, Y., Li, Y., Hou, Y. and He, J., 2022. Sanguinarine induces apoptosis in Eimeria tenella sporozoites via the generation of reactive oxygen species. Poultry Science, 101(5), pp.1–9.
Mares, M. M., Al-Quraishy, S., Abdel-Gaber, R., and Murshed, M. 2023. Morphological and Molecular Characterization of Eimeria spp. Infecting Domestic Poultry Gallus gallus in Riyadh City, Saudi Arabia. Microorganisms, 11(3), pp. 1–11.
Martins, R. R., Silva, L. J. G., Pereira, A. M. P. T., Esteves, A., Duarte, S. C., and Pena, A. 2022. Coccidiostats and Poultry: A Comprehensive Review and Current Legislation. Foods, 11(18), pp.1-20.
Masneno, A., Winarso, A. and Simarmata, Y.T.R.M.R., 2023. Literature study of coccidiosis control in ruminant livestock in dry land Nusa Tenggara Timur. Jurnal Veteriner Nusantara, 6(16), pp.1–10.
Ngwese, M. M., Manouana, G. P., Moure, P. A. N., Ramharter, M., Esen, M., and Adegnika, A. A. 2020. Diagnostic Techniques of Soil-Transmitted Helminths: Impact on Control Measures. Tropical Medicine and Infectious Disease, 5(2), pp.1–17.
Okoye, K. & Hosseini, S., 2024. Mann–Whitney U Test and Kruskal–Wallis H Test Statistics in R. In: R Programming. Singapore: Springer, pp.225–246.
Olabode, V. B., Gunya, D. Y., Alsea, U. M., Choji, T. P. P., and Barde, I. J. 2020. Histopathological Lesions of Coccidiosis Natural Infestation in Chickens. Asian Journal of Research in Animal and Veterinary Sciences, 3(1), pp.86–90.
Ortiz-Andrade, R. et al. 2020. Toxicological Screening of Four Bioactive Citroflavonoids: In Vitro, In Vivo, and In Silico Approaches. Molecules 25(24), pp. 1–12.
Pahlani, E., Wijanti, T., and Rahman, I. T. 2022. Perbandingan Profil Ekstrak Etanol Buah, Daun, dan Batang Tanaman Mengkudu (Morinda citrifolia L.). Jurnal Kesehatan Aeormedika, 8(2), pp.33–42.
Ramah, A., Saragih, H.T.B., Latif, H., Haerani, E., Amrullah, I.H., Alimuddin, A., Purnamasari, A. and Lestari, D.A., 2022. Protective effects of probiotics against tannin-induced immunosuppression in broiler chickens. Bioscience of Microbiota, Food and Health, 41(4), pp.168–176.
Ramonah, D., Pratiwi, A.D.E. and Eliza, N. 2023. Penentuan Kadar Total Fenolik dan Flavonoid Ekstrak Andrographis paniculata, Zingiber officinale dan Kombinasinya. Media Farmasi Indonesia 18(1), pp. 8–16.
Ristanti, R., Setyawan, E., Purnamasari, D., Arifin, M.I., Prabowo, M.A. and Widiastuti, Y., 2024. Anticoccidial activities of Piper betle L. essential oil on Eimeria tenella oocysts. Scientific Reports, 14(1), pp.1–12.
Scicutella, F., Mannelli, F., Daghio, M., Viti, C. and Buccioni, A. 2021. Polyphenols and organic acids as alternatives to antimicrobials in poultry rearing: A review. Antibiotics 10(8), pp.1–13.
Singh, P., Singh, J., Medhi, T., & Kumar, A. 2022. Phytochemical Screening, Quantification, FT-IR Analysis, and In Silico Characterization of Potential Bio-active Compounds Identified in HR-LC/MS Analysis of the Polyherbal Formulation from Northeast India. ACS Omega, 7, 33067 - 33078.
Vranish, J. N., Ancona, M. G., Oh, E., Susumu, K., Lasarte‐Aragonés, G., Breger, J. C., & Medintz, I. L. 2018. Enhancing coupled enzymatic activity by colocalization on nanoparticle surfaces: kinetic evidence for directed channeling of intermediates. ACS Nano, 12(8), 7911-7926.
Warditiani, N.K., Larasanty, L.P.F., Widjaja, I.N.K., Juniari, N.P.M., Nugroho, A.E. and Pramono, S., 2014. Identifikasi kandungan kimia ekstrak terpurifikasi herba sambiloto (Identification of Chemical Constituents in Purified Andrographis paniculata Herbal Extract). Jurnal Farmasi Udayana, 3(1), pp.22–25.
Xu, H., Fu, J., Luo, Y., Li, P., Song, B., Lv, Z. and Guo, Y., 2023. Effects of tannic acid on the immunity and intestinal health of broiler chickens with necrotic enteritis infection. Journal of Animal Science and Biotechnology, 14(1), pp.1–20.
Xu, H., Gong, L., Zhang, X., Li, Z., Fu, J., Lv, Z. and Guo, Y., 2025. Effects of tannic acid on growth performance, intestinal health, and tolerance in broiler chickens. Poultry Science, 104(2), pp.1–13.
Yuan, P., Xu, H., Ma, Y., Niu, J., Liu, Y., Huang, L., Jiang, S., Jiao, Yuan, X., Yang, W., and Li, Y. 2023. Effects of dietary Galla chinensis tannin supplementation on immune function and liver health in broiler chickens challenged with lipopolysaccharide. Frontiers in Veterinary Science, 10, pp.1–10.
Yuwono, E., Wandatara, R. P., Mastuti, S., and Indrasanti, D. 2023. Recent Study of Coccidiosis in Broiler Closed House: The Role of Some Aspects of Maintenance. Animal Production, 25(2), pp.99–108.
Zhao, Y.L., Wang, L., Zhang, Y., Zeng, X.X., Yin, L.L., Zhang, C.L., Li, H.Y. and Wang, Z.Z., 2020. Acute and chronic toxicity of indole alkaloids from leaves of Alstonia scholaris (L.) R. Br. in mice and rats. Natural Products and Bioprospecting, 10(2), pp.77–88.
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