Journal of Basic Medical Veterinary

Front Cover, Editorial Board, Peer Reviewers, Acknowledgments

Salma Aisyafalah, S.Si — 2024-06-05

Indexing, Subscribed Form, Guidelines for Author, Back Cover

Salma Aisyafalah, S.Si — 2024-06-05

The Effect of Giving Pork Oil and Egg Yolk as Components of a High Fat Diet on The Hematological and Cholesterol Profiles of Mice (Mus musculus)

Anissa Dhea Larasati — 2024-06-05

Background: This study examined the role of a high-fat diet, especially one containing animal fat, in its influence on the hematology of the organism, with mice as research subjects. Hematology, as the study of blood components, is the focus for analyzing the impact of a high-fat diet, which includes animal fats such as lard and egg yolks. Although fats are essential for cell function and nutrient absorption, excessive intake can increase the risk of serious health problems. Purpose: To fill the gap in knowledge by evaluating the effect of lard and egg yolk on the hematological profile of mice. Methods: True Experimental with a Pretest-Posttest Control Group design. The sampling technique used is Simple Random Sampling. The parameters yolk). The sampling technique used is Simple Random Sampling. The parameters observed include blood hematological profiles (Erythrocyte Count, Hemoglobin, and Erythrocyte Index) and cholesterol levels. Results: Expected to provide in-depth insight into the impact of high-fat diets on blood health, provide important contributions to understanding the human health implications, and support the development of more effective nutritional guidelines for managing diet and preventing metabolism-related diseases. Conclusion: It can be concluded that giving pork oil with added egg yolk can increase erythrocyte levels, hemoglobin profile, erythrocyte index (MCV and MCH), and cholesterol levels.

The Effect of Red Ginger Extract (Zingiber officinale var rubrum) on The Thickness of The Epitelial and Diameter of Seminiferous Tubules in Albino Rat (Rattus norvegicus) Exposed Monosodium Glutamat

Nogi Renaldi — 2024-06-05

Background: This study examined administration of MSG intraperitoneally to white rats resulted in a decrease in testicular weight, an increase in lipid peroxidase levels The testes, as the site of spermatogenesis, are highly vulnerable to oxidation processes caused by free radicals, which can disrupt spermatogenesis. Free radicals can be countered by antioxidants. One plant believed to have antioxidant activity is red ginger. Purpose: To determine the effect of red ginger against histopathology seminiferous tubules of white rat (Rattus norvegicus) exposed by monosodium glutamate. Methods: This study used 30 rats which were divided into 5 groups. The positve control group (K+) were given only monosodium glutamate 5g/kgBW and CMC Na orally, the treatments groups P1 were given monosodium glutamte 5g/KgBW + red ginger 50mg/rat/day, P2 were given 5g/KgBW + red ginger 100mg/rat/day, and P3 were given 5g/KgBW + red ginger 200mg/rat/day. All treatments were administered for 42 days. After 42 days of treatments, all rats were sacrificed and seminiferous tubules were observed with microscope from pathology laboratory. Results: Administration of red ginger extract can defend seminiferous tubules epithelial thicknes and diameter from exposure of monosodium glutamate with 100mg/KgBW dosage as the most effective dosage. Seminiferous tubules epitelial thickness and diameter were tested using one-way ANOVA and Duncan's multiple range test. Conclusion: Red Ginger Extract (Zingiber officinale var rubrum) has shown potential effects to counteract Monosodium Glutamat consequences on The Thickness of the epitelial and diameter of seminiferous tubules of white rat (Rattus norvegicus).

Differences in Blood Glucose Levels of Rats (Rattus norvegicus) Given Sugar Cane Juice and Cyclamate Solution as a Sweetener

Virginia Ayu Kinanti — 2024-06-05

Background: Artificial sweeteners can cause glucose intolerance, which is a condition where the glucose in the blood is abnormal. Purpose: This research was conducted to determine the difference in blood glucose levels of mice as experimental animals that were given sugarcane juice as a natural sweetener and cyclamate solution as an artificial sweetener. Methods: The research method applied was laboratory experimental followed by checking blood glucose levels using a GCU easy touch glucometer and the data analysis method used was descriptive analysis. Results: the average blood sugar before treatment was P0 (control) 110 mg/dl, P1 (test animals given sugarcane juice solution) 63 mg/dl, P2 (test animals given sodium cyclamate solution) 69.5 mg/dl . Then, the average blood sugar after treatment was, P0 (control) 77.5 mg/dl, P1 (test animals given sugar cane juice) 81.5 mg/dl, P2 (test animals given cyclamate solution) 101.5 mg/dl etc. From this data, it was obtained that the level of glucose in the blood decreased by an average of 32.5 for P0. Then, there was an increase in blood glucose levels with an average of 18.5 for P1. In addition, there was an increase in blood glucose levels with an average of 32 for P2. Blood sugar levels in the treatments given cyclamate and sugar cane juice both experienced an insignificant increase in blood sugar, but within two weeks the average increase in blood sugar was highest in the treatment given cyclamate. Conclusion: There are differences in blood glucose levels of mice as experimental animals given sugarcane juice as a natural sweetener and cyclamate solution as an artificial sweetener.

The Potency of Antidiabetic of Psidium guajava Fruit Extract Against Streptozotocin-induced Type 2 Diabetic Rats

Safira Salsabila Citagami — 2024-06-05

Background: The chronic disease known as diabetes mellitus is brought on by either the pancreas's inability to make enough insulin or the body's inability to use it. Purpose: This plant finds applications for treating diarrhea, dysentery, gastroenteritis, hypertension, diabetes, caries and pain relief. Purpose: The current study aimed to determine how Psidium guajava fruit extract affected the blood glucose, body weights, and insulin levels of streptozotocin (STZ)-induced diabetic rats over 21 days. Methods: The extract's effectiveness was compared to that of glibenclamide, a common hypoglycemic medication. 30 male Wistar albino rats were divided into 5 groups with 6 animals in each group. V: Normal control (Group-I), diabetic control (Group-II), diabetic rats treated with glibenclamide 0.6mg/kg bw (Group-III), diabetic rats treated with Psidium guajava fruit extract 200 mg/kg bw (Group-IV) and Psidium guajava fruit extract 400 mg/kg bw (Group-V). All group of rats were subjected to evaluation of body weight, blood glucose and serum insulin levels on day 0, 7, 14 and 21 of the experiment. Results: There was significant (P<0.05) decrease in body weight and serum insulin and significant (P<0.05) increase in blood glucose level in Group-II compared to Group-I rats. In the Present study, daily oral administration of Psidium guajava fruit extract at dose rate of 200 and 400 mg/kg bw and glibenclamide at 0.6mg/kg bw in diabetic rats for 21 days showed a progressive improvement in body weight, blood glucose and serum insulin concentration. Conclusion: It can therefore be concluded the results of this study indicate that Psidium guajava fruit extract possesses anti-diabetic properties in Wistar albino rats.

Protective Effects of Thymoquinone on Endothelial Cell Dysfunction in Hypercholesterolemia

Giftania Wardani — 2024-06-05

Background: Increased reactive oxygen species (ROS) have been implicated as important mechanisms that contribute to endothelial dysfunction (ED). The administration of thymoquinone in animal models significantly inhibits ROS production. Purpose: The protective effects of thymoquinone on endothelial cell dysfunction were studied in cholesterol-fed rabbits. Thirty rabbits were randomly divided into five groups. Methods: The negative control group was fed a standard diet, the positive control group was fed the same diet with 2 % cholesterol, the Thymoquinone group was fed the same diet with 2 % cholesterol and Thymoquinone 100 mg/Kg BW/day, 200 mg/Kg BW/day or 400 mg/Kg BW/day. Results: The cholesterol-rich diet significantly increased Malondialdehyde (MDA) in the aortic blood vessels, as reflected by Thiobarbituric Acid-Reactive Substances (TBARS), inhibited endothelium-dependent vascular relaxations to acetylcholine and decrease cyclic GMP were compared with vessels from normal rabbits (negative control). In cholesterol-fed rabbits, Thymoquinone treatment decreased MDA in plasma production, improved endothelium-dependent relaxations to acetylcholine and increase cyclic GMP production. Conclusion: These results suggest that dietary treatment of rabbits with thymoquinone may prevent superoxide anion (O2-) induced inactivation of endothelium-dependent relaxing factor (EDRF), improve the endothelium-dependent relaxation to acetylcholine in the aortic blood vessels, and increase cyclic GMP content in aortic of cholesterol-fed rabbits.

Isolation and Identification of Bacterial Biosurfactant Activity from Mangrove Sediments

Rahmi Sugihartuti — 2024-06-05

Background: Ujung Pangkah mangroves are reported to have been contaminated with heavy metals. Such heavy metals can induce microorganisms to produce biosurfactants. Biosurfactants with amphiphilic characteristics can lower surface tension. Biosurfactants can be used as antibacterial, antifungal, and antiviral for biomedical purposes. Purpose: This study aimed to identify and test the activity of biosurfactant isolates of bacteria from Ujung Pangkah Mangrove sediments, Gresik Regency. Methods: Biosurfactant activity test methods include emulsification index, oil spreading, drop collapse, and parafilm test. Results: The results of the identification of bacterial isolates in this study obtained the genus Bacillus sp. because bacterial isolates show rod shape, Gram-positive, aerobic, and have ellipse-shaped endospores on the subterminal. The results of the biosurfactant activity test with the Emulsification index method showed an average result of 54.39% and the results of the biosurfactant activity test with the oil spreading method showed that there was a clear zone. The average result of the clear zone obtained is 54.83 mm. The average result in the parafilm test was 8.02 mm and the drop collapse test showed positive results characterized by falling and spreading of bacterial isolate fermentation broth supernatants.