Jurnal Medik Veteriner 2022-06-04T16:49:35+00:00 Faisal Fikri, drh., M.Vet. Open Journal Systems <p><strong>WELCOME</strong> to Open Journal Systems (OJS) Jurnal Medik Veteriner (JMV).</p> <p>Jurnal Medik Veteriner (JMV), with registered number pISSN <strong><a href="" target="_blank" rel="noopener">2615-7497</a></strong> ; eISSN <strong><a href="" target="_blank" rel="noopener">2581-012X</a></strong> is a national journal published by <strong><a href="" target="_blank" rel="noopener">Faculty of Veterinary Medicine, Universitas Airlangga</a></strong><strong> </strong>and<strong> </strong><strong><a href="" target="_blank" rel="noopener">Indonesian Veterinary Medical Association (IVMA)</a>.</strong></p> <p>Jurnal Medik Veteriner (JMV) has been <strong><a title="SINTA Ristekdikti" href="" target="_blank" rel="noopener">accredited by the Directorate General of Research and Development, Ministry of Research, Technology and Higher Education, Republic of Indonesia</a></strong> Number: 28/E/KPT/2019, effective since September 26, 2019.</p> <p>Jurnal Medik Veteriner (JMV) publishes high quality and novelty papers focusing on Veterinary and Animal Science. The fields of study are anatomy, pathology, basic medicine, veterinary public health, microbiology, veterinary reproduction, parasitology, animal husbandry, and animal welfare. Food animals, companion animals, equine medicine, aquatic animal, wild animals, herbal medicine, acupuncture, epidemiology, biomolecular, forensic, laboratory animals and animal models of human infections are considered. Jurnal Medik Veteriner (JMV) published two times a year: April and October.</p> Front Cover, Editorial Board, Peer Reviewers, Acknowledgments 2022-06-04T16:48:36+00:00 Choirun Nisa, SIIP. 2022-06-04T00:00:00+00:00 Copyright (c) 2022 Choirun Nisa, SIIP. Indexing, Subscribed Form, Guidelines for Author, Back Cover 2022-06-04T16:49:35+00:00 Choirun Nisa, SIIP. 2022-06-04T00:00:00+00:00 Copyright (c) 2022 Choirun Nisa, SIIP. The Effectiveness of Time Equilibration Before Freezing in Sapera Goat Spermatozoa After Electric Separating Sperm 2022-03-01T00:44:37+00:00 Amung Logam Saputro Ragil Angga Prastiya Meidina Zulva Ulinuha Prasita Widayani <p><em>The purpose of this study was to determine the effectiveness of time equilibration before freezing sapera goat spermatozoa after ESS (Electric Separating Sperm) on motility, viability, membrane integrity, normality and abnormality of spermatozoa. Sapera goat spermatozoa were collected using an artificial vagina and then separated using the ESS method. This study used a completely randomized formula with three treatments, which are the equilibration time of P1 (1 hour), P2 (3 hours), and P3 (4 hours), with six replications. The analysis in this study used the ANOVA test followed by the Duncan test to determine the real difference between treatments. The conclusion of this study showed the highest percentage of motility, viability, membrane integrity, normality of sapera goat spermatozoa at 1-hour treatment, for the lowest percentage of abnormalities at 1-hour treatment. Significantly different results (p &lt;0.05) on the anode and cathode sides in all equilibration time treatments.</em></p> 2022-04-22T00:00:00+00:00 Copyright (c) 2022 Amung Logam Saputro, Ragil Angga Prastiya, Meidina Zulva Ulinuha, Prasita Widayani The Association of Age and Number of Parturition with Mammary Gland Tumor Case Rate in Mice in Malang Raya 2022-03-01T10:10:40+00:00 Maulidi Robingi Mardiyani Wukirani Essly Hervianingsih Adha Sang Ayu Putri Aristya Dewi Ani Setianingrum Dyah Ayu Oktavianie Ardhiana Pratama Andreas Bandang Hardian <p><em>Mammary gland tumours are frequently reported in adult female mice. It broadly affects the productivity and breeding performance of mice in breeding centres. The tumours might occur spontaneously due to internal factors such as genetic mutation and hormonal disturbance, however, external causes such as Mouse Mammary Tumour Virus (MMTV) infection was also reported. This study aimed to prove that age and number of parturition associate with mammary gland tumour case rate in female mice in Malang Raya. Chi-square tests were employed to analyse the association between risk factors and mammary gland tumour case rate in this study. Histopathologic analysis described three subtypes of mammary gland tumours in mice which were adenoma, adenocarcinoma type A and adenocarcinoma type B. Chi-square tests results of tumour case rate toward age and number of parturitions were consecutively 0,004 and 0,0025 which indicated that age and number of parturitions were associated with mammary gland tumours case rate in mice in Malang Raya. The mapping of tumour case distribution visually showed a trend that higher disease proportions were spotted in higher altitude of sampling location. This might relate to the geographical breeding characteristics or temperature which needs further study to explore the association of spatial factors with the mammary tumour incidence in mice.</em></p> 2022-04-22T00:00:00+00:00 Copyright (c) 2022 Maulidi Robingi Mardiyani Wukirani, Essly Hervianingsih Adha, Sang Ayu Putri Aristya Dewi, Ani Setianingrum, Dyah Ayu Oktavianie Ardhiana Pratama, Andreas Bandang Hardian Difference in Lairage Time Before Slaughtering on Malondialdehyde (MDA) Levels in Landrace Pig Blood Serum 2021-08-17T07:46:12+00:00 Putri Jauza Aulia Muhandis Nusdianto Triakoso Amung Logam Saputro <p><em>Lairage time before slaughtering a pig can recover the stress caused by transportation from the farm to the abattoir. Stress conditions can overcome the imbalance between Reactive Oxygen Species (ROS) and the ability of the system to be easily detoxified. Malondialdehyde (MDA) is a specific product of lipid peroxidation, it is often used as a biomarker in research. A total of 18 pigs were divided into two groups, pigs with a lairage time of 6-8 hours and a rest period of 12-24 hours, MDA levels were tested using the Thiobarbituric Acid Reactive Substance (TBARS) method and then analyzed. There was a significant difference (p&lt;0.05) between the group lairage time length of 6-8 hours (18.10 ± 11.616) with a rest period of 12-24 hours (6,80 ± 4,852), there was a decrease in stress levels and was marked by a decreased in levels of MDA in pigs that were rested for 12-24 hours before being slaughtered.</em></p> 2022-04-22T00:00:00+00:00 Copyright (c) 2022 Putri Jauza Aulia Muhandis, Nusdianto Triakoso, Amung Logam Saputro Morphometry Identification of Lice on Male Javan Langur (Trachypithecus auratus) 2022-03-01T10:10:34+00:00 Nurina Titisari Yunita Cahyaningrum Reza Yesica Nofan Rickyawan <p><em>A rehabilitation center for Javan Lutung animals in East Java carries out routine checks on rehabilitated animals to prevent diseases that can affect health. Diseases that can infect these endangered animals are not only diseases that are inside the body but also outside the body such as lice infestation. The purpose of this study was to identify the species of louse that attack Javan langurs in an effort to increase information on ectoparasite species in these endemic animals. The sample was obtained from an adult Javan langur, then the sample was preserved by the unstained Slide mounting method. The results were observed using a microscope and body length was measured using the ImageJ software. The results showed that the ectoparasite shape has a flat-dorsoventral body with a total body length of 1.65 mm. From morphometry and morphological characteristics, the samples identified were the male species of Pedicinus ancoratus.</em></p> 2022-04-22T00:00:00+00:00 Copyright (c) 2022 Nurina Titisari, Yunita Cahyaningrum, Reza Yesica, Nofan Rickyawan Comparison of Antigenicity Between Local and Massachusetts Strains of Infectious Bronchitis Virus using Indirect ELISA Test 2022-03-16T08:33:09+00:00 Jola Rahmahani Daniswara Irwanza Akbar Suwarno Suwarno <p><em>The aim of this study was to compare the antigenicity between the whole virus and protein S of both local strain I-147 and Massachusetts strain of Infectious bronchitis virus with indirect ELISA. We used 10 positive serums from post vaccinated chickens for further reaction with four groups of antigens in this study that are: P1) S protein (spike glycoprotein) antigen of local strain IB virus; P2) S protein (spike glycoprotein) antigen of Massachusetts strain IB virus; P3) whole virus antigen of local strain IB virus; P4) whole virus antigen of Massachusetts strain IB virus. The results of this research are Optical Density (OD) values of each treatment, then to be analyzed using One Way ANOVA and followed by Duncan Multiple Range Test 5%. The results show that there are differences between the antigenicity of the whole virus and S protein in both local strain and Massachusetts strain of IB virus. The whole virus antigen of both strains has a significant difference (p&lt;0.05) with the S protein antigen in both strains. Specifically, the whole virus antigen of Massachusetts strain has the highest OD among the other groups.</em></p> 2022-04-22T00:00:00+00:00 Copyright (c) 2022 Jola Rahmahani, Daniswara Irwanza Akbar, Suwarno Betanodavirus Infection in Barramundi in Riau Islands 2022-03-01T10:08:19+00:00 Achmad Bahtiar Rifai Titis Wulandari Desi Surastini Pramudya Dwi Wahyu Irawanto <p><em>Diseases that can cause very high economic losses are viruses and one of them is Betanodavirus or commonly called Viral Nervous Necrosis (VNN). VNN is a disease registered by The Office International des Epizooties (OIE) and is a major problem in marine fishery production in the world. This virus is able to infect fish from larva size to consumption size in a short period of time to reach death with a frequency of 80% to 100%. The disease can infect barramundi and is spread throughout the Riau Islands. This study used a live fish sampling method showing VNN signs and freshly dead fish. The research samples were obtained from several cities and districts in the Riau Islands Province, including Batam City, Bintan Regency, and Tanjung Balai Karimun Regency, the sample selection was based on clinical symptoms and fish mortality. Then the samples were necropsied for eye and brain organs, then amplified using primers targeting the RNA 2 gene. The results showed that VNN had been detected in the Riau Islands Province, i.e. Batam City, Bintan Regency, and Tanjung Balai Karimun Regency.</em></p> 2022-04-22T00:00:00+00:00 Copyright (c) 2022 Achmad Bahtiar Rifai, Titis Wulandari, Desi Surastini, Pramudya Dwi Wahyu Irawanto An Efficacy of Seligi Leaf Flour Fermentation on Cholesterol Levels, Low Density Lipoprotein, and High Density Lipoprotein in Catfish 2022-03-16T08:41:01+00:00 Reza Hardiansyah Mirni Lamid <p><em>Catfish</em><em> is a freshwater fish with a high economic value, so it is very good to be developed. Feed is one of the most important components in the cultivation process because it plays a role in supporting the plants and the survival of cultivated fish. Seligi (Phyllanthus buxifolius) is a native Indonesian medicinal plant that has been widely used for medicinal purposes. Seligi leaf extract is known to contain flavonoids, polyphenols (tannins), saponins, alkaloids, quinones, and triterpenoid steroids. The purpose of this research was to determine the effect of using fermented seligi leaf flour in feed to reduce levels of total cholesterol, low-density lipoprotein, and high densities lipoprotein in catfish meat. The method used in this research is the experimental method. The main parameters in this study were total cholesterol, low-density lipoprotein (LDL), and high-density lipoprotein (HDL) in catfish meat. The supporting parameters in this research are water quality which includes temperature, pH, dissolved oxygen (DO), and ammonia (NH3-N). The results showed that the total cholesterol content of catfish meat ranged from 116.84-233.65 mg/dL. Meanwhile, the results of LDL in catfish meat ranged from 37.89-74.22 mg/dL. Then, the content of HDL in catfish meat ranges from 89.25-225.87 mg/dL.</em></p> 2022-04-22T00:00:00+00:00 Copyright (c) 2022 Reza Hardiansyah, Mirni Lamid The Risk Factor of Subclinical Mastitis Incident in Dairy Cattle in KPSP Ijen Makmur, Banyuwangi 2021-12-22T16:53:50+00:00 Indah Puspita Ningrum Soeharsono Soeharsono Prima Ayu Wibawati Yeni Dhamayanti Maya Nurwartanti Yunita <p><em>Mastitis is an inflammatory disease in the internal tissue of the mammary gland. Subclinical mastitis does not show any changes in the shape of the mammary gland and to detect it can be examined the number of somatic cells in milk. Subclinical mastitis uses the California Mastitis Test (CMT) method as preliminary research to obtain preliminary data on the incidence of subclinical mastitis in KPSP Ijen Makmur, Licin District, Banyuwangi Regency. This research is descriptive research. The detection for this research was carried out by taking milk samples in cows per purposive and reacted CMT reagents issued circularly for 10-15 seconds. Observation of results is determined based on changes inconsistency with the existence of gel and the results of interview farmers as supporting data. The results showed that the influencing factors incidence of subclinical mastitis in KPSP Ijen Makmur, Licin District, Banyuwangi Regency were milk production, age and amount of lactation. In contrast, the biggest factor for reducing the risk of subclinical mastitis is udder health followed by body conditions measured by Body Condition Score.</em></p> 2022-04-22T00:00:00+00:00 Copyright (c) 2022 Indah Puspita Ningrum, Soeharsono, Prima Ayu Wibawati, Yeni Dhamayanti, Maya Nurwartanti Yunita Service per Conception, Conception Rate, Calving Rate and Non-Return Rate in Beef Cattle in Kalipuro, Banyuwangi 2022-03-16T08:40:48+00:00 Ahmad Rajul Dinul Tjuk Imam Restiadi Prima Ayu Wibawati Hermin Ratnani Amung Logam Saputro Ragil Angga Prastiya <p><em>This study aimed to determine the number of reproductive efficiency parameters including service per conception, conception rate, calving rate, and non-return rate for broilers in Kalipuro District, Banyuwangi Regency. The secondary data was collected from the Banyuwangi District Agriculture Office. This research is also intended to dissect factors related to the S/C, CR, CvR, and NRR figures with descriptive analysis, including bio reproduction mastery factors, service factors, and feed factors. The primary data was collected from beef cattle breeders in Kalipuro District, Banyuwangi Regency. In addition, there were IB implementation factors whose data were obtained from questionnaires to inseminators in Kalipuro District, Banyuwangi Regency. The number of S/C broilers in Kalipuro Subdistrict, Banyuwangi Regency was good maintenance, in January-March 2020 of 1.71, 1.99, and 1.74, meanwhile, the number of CR were 58%, 50% 57%, CvR for January-March were 44%, 84%, 53% and NRR for January, February and March were 41%, 49%, and 46%, respectively, were below the standard rate.</em></p> 2022-04-22T00:00:00+00:00 Copyright (c) 2022 Ahmad Rajul Dinul, Tjuk Imam Restiadi, Prima Ayu Wibawati, Hermin Ratnani, Amung Logam Saputro, Ragil Angga Prastiya The Steroid Hormone Profile in Etawah Crossbreed Goat While Ovulation Induced using The Selectsynch Method 2021-05-20T17:14:13+00:00 Muhammad Aftabuddin Rz Pudji Srianto Chairul Anwar Nidom Sri Pantja Madyawati Trilas Sardjito Ismudiono Ismudiono <p><em>This study aimed to provide alternative information and solutions in an effort to increase reproductive productivity in etawah crossbreed goats (PE). The sample used in this study was 10 female PE with an average age of 2.5 - 3 years and primiparous at least . Ovulation induction was performed using 0.1 mg intra-muscular Gonadotropin-Releasing Hormone (GnRH), after seven days injection of Prostaglandin F2α (PGF2α) was given as much as 2.5 mg submucosa of the vulva, followed by a second injection of GnRH as much as 0,2 mg intramuscular in samples that have really experienced heat and selected selectively according to the signs of natural heat shown by the sample and followed by insemination as much as 2 doses or 0.50 ml of frozen semen. Blood sampling was performed at H0, H7<sup>th</sup>, H14<sup>th</sup> and H21<sup>th</sup> after insemination. All blood samples were collected and progesterone and estrogen hormone profiles were examined using the ELISA method. From the results of the ELISA test, the mean progesterone hormone profile H0 = 4.798 ng / ml, H7<sup>th</sup> = 4.887 ng / ml, H14<sup>th</sup> = 4.824 ng / ml, H21<sup>th</sup> = 5.148 ng / ml. The profile of the hormone estrogen at H0 = 19,461 pg / ml, H7<sup>th</sup> = 17,457 pg / ml, H14<sup>th</sup> = 18,248 pg / ml, H21<sup>th</sup> = 17,515 pg / ml. This study showed an increase in the levels of the progesterone hormone at H0 to H7<sup>th</sup>, then slightly decreased in H14<sup>th</sup> and a significant increase in H21<sup>th</sup>. For the estrogen hormone, there is a decrease from H0 to H7<sup>th</sup> then there is a slight increase in H14<sup>th</sup> and decreases again in H21<sup>th</sup>.</em></p> 2022-04-22T00:00:00+00:00 Copyright (c) 2022 Muhammad Aftabuddin Rz, Pudji Srianto, Chairul Anwar Nidom, Sri Pantja Madyawati, Trilas Sardjito, Ismudiono Probiotic Potential of Lactic Acid Bacteria on Feed Efficiency, Weight and Carcass Percentage in Ducks 2022-03-16T08:41:13+00:00 Evania Haris Chandra Widya Paramita Lokapirnasari Sri Hidanah Muhammad Anam Al-Arif Wiwik Misaco Yuniarti Epy Muhammad Luqman <p><em>The aim of the study was to determine the effect of the use of probiotic lactic acid bacteria (</em><em>Lactobacillus acidophilus, Lactobacillus casei, Lactococcus lactis, and Bifidobacterium sp) on feed efficiency, carcass weight and carcass percentage in broiler ducks. Probiotics lactic acid bacteria produce bacteriocins and organic acids that inhibit the growth of pathogenic microbes in the digestive tract of poultry, so that the intestinal villi of broiler ducks are healthy. Besides that, probiotics are able to reduce cholesterol levels in meat when consumed by humans and can improve health. This study used 48 peking ducks. The probiotics of Lactic Acid Bacteria used were a concentration of 1.2 x 10<sup>8</sup> CFU/ml. In this study there were four different treatments, namely P0 (without probiotics), P1 (given 1 ml probiotics/liter drinking water), P2 (given 2 ml probiotics/liter drinking water) and P3 (given 3 ml probiotics/liter of drinking water). The results of the feed efficiency study showed that there was a significant difference (p&lt;0.05) in the P0, P1, P2 and P3 treatments. The highest feed efficiency was at P3 (33.81%) while the lowest was P0 (30.84%). The results of the study on carcass weight indicated that the probiotics tended to be higher than those who were not given probiotics but statistically not significantly different (p&gt;0.05). The highest carcass weight was P3 (1749.92 g) and the lowest was P0 (1709.25 g). The results of the study on the percentage of carcass weight showed a significant difference (p&lt;0.05) in both P0, P1, P2 and P3 treatments. The highest percentage of carcass weight was at P3 (64.60%) and the lowest was P0 (60.96%). It can be concluded that the administration of probiotics lactic acid bacteria (Lactobacillus acidophilus, Lactobacillus casei, Lactococcus lactis, and Bifidobacterium sp) at a dose of 1 ml/liter of drinking water, 2 ml/liter of drinking water and 3 ml/liter of drinking water can affect feed efficiency and the percentage of duck carcasses. but has no effect on the carcass weight of broiler ducks.</em></p> 2022-04-22T00:00:00+00:00 Copyright (c) 2022 Evania Haris Chandra, Widya Paramita Lokapirnasari, Sri Hidanah, Muhammad Anam Al-Arif, Wiwik Misaco Yuniarti, Epy Muhammad Luqman Study of Performance Index and Business Analysis on Chicken Infected by Escherichia coli with Probiotic Provision of Lactic Acid Bacteria 2022-03-16T08:40:55+00:00 Farid Abdurrahman Soeharsono Soeharsono Koesnoto Soepranianondo <p><em>This study was conducted to determine the performance index and business analysis of broilers infected with Escherichia coli by administering lactic acid bacteria probiotics in the form of Lactoped<sup>®</sup> (Lactobacillus casei, Lactobacillus plantarum, Lactobacillus fermentatum, Lactobacillus acidophilus). This type of research was true experimental research using a post-test-only control group design. The design used in this study was a completely randomized design (CRD). 30 broilers were randomized into three treatments (P0, P1 and P2) in each group with a minimum of six times. Probiotic lactic acid bacteria (LAB) with a concentration of 108 CFU/ml in broilers infected with Escherichia coli showed a higher performance index. It could cover the entire fixed costs so still feasible to do, meanwhile, for the scale of research (10 heads/treatment), it was not yet feasible to implement due to it has suffered losses.</em></p> 2022-04-22T00:00:00+00:00 Copyright (c) 2022 Farid Abdurrahman, Soeharsono, Koesnoto Soepranianondo Risk Factors and Scabious Prevalence in Cats During 2020 in Griya Satwa Clinic, Magetan 2021-12-22T16:54:09+00:00 Nedriana Cahya Hardany Primarizky Maya Nurwartanti Yunita <p><em>Scabious is a disease that often infests cats, originating from the family Sarcoptidae. This study aimed to determine the prevalence and factors that can affect the risk of scabious in cats. The study was evaluated at the Griya Satwa Clinic, Magetan in January-February 2021. The design of this study was a retrospective cohort study by taking secondary data during the 2020 period as study material. The data obtained were then entered into the Statistical Package for the Social Sciences (SPSS) version 20 for windows to analyze the association's relationship and increased risk by calculating the Chi-Square and Odds Ratio. The samples obtained were 76 cats infested with scabious. The analysis of the association of risk factors for scabious was breed (P = 0.428), age (P = 0.793), sex (P = 0.426). The results of the prevalence of scabious in cats from the study during January-February 2021 in the 2020 period were 9.5%, and the analysis of the association (Chi-Square) and the increased (Odds Ratio) of factors including breed, age, sex on the risk of scabious in cats were not significance / absent influence, The probability of error obtained is outside the tolerance set by the researcher, so the results are not significant (p&gt;0,05).</em></p> 2022-04-22T00:00:00+00:00 Copyright (c) 2022 Nedriana Cahya, Hardany Primarizky, Maya Nurwartanti Yunita Business Analysis of Probiotic Administration of Lactic Acid Bacteria on The Performance of Kampung Super Chicken 2022-03-16T08:41:19+00:00 Gogik Satrio Margo Utomo Sri Hidanah Muhammad Anam Al Arif Widya Paramita Lokapirnasari Wiwik Misaco Yuniarti <p><em>This study aimed to determine the effect of lactic acid bacteria probiotics on the performance of kampung super chicken. Kampung super chicken is the result of a cross between a layer chicken and a superior kampung super chicken. A total of 48 kampung super chickens aged 14 days were randomized into 4 treatments and each treatment consisted of 12 chickens. This study was conducted for 1 week of adaptation and 5 weeks of treatment. This study used 4 different treatments including P0 not using probiotics, P1 using probiotics as much as 1 ml/liter drinking water, P2 using probiotics as much as 2 ml/liter drinking water and P3 using probiotics as much as 4 ml/liter drinking water. In conclusion, based on the results of the Analysis of Variance (ANOVA) showed that p&lt;0.05, which means that the data had a significant difference in the final weight increase, carcass weight increase and carcass weight percentage to the control treatment.</em></p> 2022-04-22T00:00:00+00:00 Copyright (c) 2022 Gogik Satrio Margo Utomo, Sri Hidanah, Muhammad Anam Al Arif, Widya Paramita Lokapirnasari, Wiwik Misaco Yuniarti Estrogen Concentration on Friesian Holstein Crossbred with Supplementation Feed Cassava Peel 2021-06-12T15:20:55+00:00 Akhmad Baihaqi Zulfarniansyah Erma Safitri <p><em>The purpose of this study was to determine the ratio of estrogen concentrations given cassava peel feed supplementation. A total of 24 cows of Friesian Holstein, weighing 250-300 kg were divided into two groups and each group contains 12 replications. The first group is Friesian Holstein breeds with the condition that they are given elephant grass feed ad libitum and 25 kg of tofu dregs. The second group is Friesian Holstein breeds with the condition that they are fed ad libitum elephant grass, 25 kg of tofu dregs, and 25 kg of cassava peel. The milk samples were then taken to be tested for estrogen levels using the direct competitive ELISA. Analysis of estrogen concentration data was then carried out by normality test using Shapiro Wilk test then followed by the Paired T-test with a significance level of 5%. Based on the Paired T sample correlation test was 0.072 (p&gt;0.05), which means that the feed gave the cassava peel and not the cassava peel was negatively associated. Based on the Paired T-test is 0.193 (p&gt;0.05), which means there is no significant relationship between the 2 test groups. It can be concluded that there is no significant difference in the estrogen concentration of Friesian Holstein cattle fed with and without cassava peel supplementation.</em></p> 2022-04-22T00:00:00+00:00 Copyright (c) 2022 Akhmad Baihaqi Zulfarniansyah, Erma Safitri Immunogenicity of Local and Massachusetts Strains Infectious Bronchitis Virus 2022-03-16T08:35:39+00:00 Jola Rahmahani R Zaksara Fero Ali Mutaqin Wudhu Suwarno Suwarno Martia Rani Tacharina <p><em>This study aimed to compare the immunogenicity between the whole virus and protein S of both local strain I-147 and Massachusetts strain of Infectious bronchitis virus with indirect ELISA. 24 healthy mice are divided into four groups with six mice in each group that are: P1) a group of mice that are vaccinated with S protein (spike glycoprotein) of local strain Infectious Bronchitis virus; P2) a group of mice that are vaccinated with S protein (spike glycoprotein) of Massachusetts strain Infectious Bronchitis virus; P3) a group of mice that are vaccinated with the whole virus of local strain Infectious Bronchitis virus; P4) a group of mice that are vaccinated with the whole virus of Massachusetts strain Infectious Bronchitis virus. The results of this research are Optical Density (OD) values of each treatment, then to be analyzed using One Way ANOVA and followed by Duncan Multiple Range Test 5% and T-test. The results show that there are differences in immunogenicity between the whole virus and S protein in both the local strain and Massachusetts strain of Infectious bronchitis virus. The OD value of the whole virus in the two strains has a significant difference from the OD value of the protein S antigen in both strains, both in the serum taken at week 2 and week 4 post-vaccination.</em></p> 2022-04-22T00:00:00+00:00 Copyright (c) 2022 Jola Rahmahani, R Zaksara Fero Ali Mutaqin Wudhu, Suwarno, Martia Rani Tacharina Characterization of Gene Coding Fusion Protein of Newcastle Disease Virus Infected in Native Chicken in Surabaya 2022-02-01T08:03:11+00:00 Maha Kirana Rahaju Ernawati Jola Rahmahani Fedik Abdul Rantam <p><em>This study aimed to discover the homology of nucleotide sequence, homology percentage, and those relations phylogenetic of protein Fusion (F) gene coding of Newcastle disease in domestic chicken (Gallus gallus domesticus) in Surabaya using some comparison isolate from GenBank. Samples were scoured of digestive organs from native chicken, that was collected from a traditional market in Wonokromo, Surabaya. Samples were tested using RT-PCR with primer forward and reverse with target 976bp, a positive sample which is continued with sequencing then homology and nucleotide analysis which is done and which is translated into amino acid. The result of homology chc/SBY/2018 sample has a similarity of 88% with references sequence, while with Lasota vaccine has a similarity of 87%, and the highest result of homology showed by the comparison with various isolates in Indonesia 90-95%. Translation results from nucleotide alignment into amino acid showed shifts in amino acid structure, which is amino acid shifts could be affected by many things like nutrition, wheater, environment, etc. The conclusion was chc/SBY/2018 sample has a quite high similarity with Indonesian isolates and undergoes mutation on nucleotide structure on amino acid and phylogenetic analysis. This study related to some isolates of vaccine and some isolates in Indonesia.</em></p> 2022-04-22T00:00:00+00:00 Copyright (c) 2022 Maha Kirana, Fedik Abdul Rantam, Rahaju Ernawati, Jola Rahmahani Surgical Procedure for Pyometra and Mammae Tumor Treatment in a Pitbull Dog 2022-02-12T04:17:43+00:00 Nofan Rickyawan Cheptien Winda Virgiantari Muhamad Arfan Lesmana Dian Vidiastuti <p><em>Pyometra is an infection or inflammation of the uterine wall characterized by the accumulation of pus in the uterine lumen. Predisposing factors include age, breed, bacterial infection, and hormones. Another disease that is often correlated with an imbalance in reproductive hormones is mammary tumors. A 7-year-old unspayed female Pitbull dog weighed 27.9 kg has clinical symptoms of frequent discharge of mucopurulent reddish-brown discharge with a foul odor from the vagina. The left mammary glands of the second, third, and fourth nipples have lumps about 20 cm in diameter, are solid in consistency, well-defined, the same color as the skin surface, and can be moved. Diagnosis performed on physical examination, hematology, blood chemistry, and cytology was pyometra and mammary tumors. Treatment was done by ovariohysterectomy and unilateral mastectomy. Post-surgery therapy was enrofloxacin 5 mg/kg, ketoprofen 2 mg/kg, phytomenadione 1 mg/kg, and Biodin<sup>®</sup> 0.1 ml/kg, followed by intensive surgical wound care. The patient recovered after two months of treatment.</em></p> 2022-04-22T00:00:00+00:00 Copyright (c) 2022 Nofan Rickyawan, Cheptien Winda Virgiantari, Muhamad Arfan Lesmana, Dian Vidiastuti Capillaria spp. in a Reticulated Python (Python reticulatus) in Banyuwangi Reptile Community 2022-03-16T08:40:42+00:00 Sayyida Kamila Dini Nusdianto Triakoso Amung Logam Saputro Aditya Yudhana <p><em>Reptiles as exotic pets become more commonly desired by society, one of which is the reticulated python. Pythons kept as pets also have a risk of disease infection including helminthiasis. Worm parasite infects snake by the oral route. Some snake feed including frogs and mice can act as an intermediate host for worm parasites. A male reticulated python was kept by a member of the Banyuwangi reptile community and was presented with healthy and normal eating habits. This case study was to determine the worm parasite in the wild-caught reticulated python kept as an exotic pet. Fresh fecal samples were stored in a container with 70% alcohol added as a preservative. The sample was then evaluated in the laboratory and examined using the native method, floatation method, and simple sedimentation method. As result, under the microscope to be the Capillaria spp. the egg was observed.</em></p> 2022-04-22T00:00:00+00:00 Copyright (c) 2022 Sayyida Kamila Dini, Nusdianto Triakoso, Amung Logam Saputro, Aditya Yudhana Medical Treatment for Pyometra in Cat 2022-03-08T17:25:09+00:00 Tantri Dyah Whidi Palupi Tri Wahyu Suprayogi Ismudiono Ismudiono <p><em>The Pyometra case is a case that is often found in Female Cat. This case report will discuss the handling of a case of pyometra in a 7-year-old mixed breed cat who is being treated at Harmoni Pet Care, Menanggal, Gayungan, Surabaya, Indonesia. Reports from the owner that these cats are still in good appetite but sanguino-purulent discharge from the vagina was found. In clinically diagnosed with a pyometra, the treatment used to treat pyometra case was ovariohysterectomy. Postoperative care was carried out by administering antibiotics (Clanexi; Amoxicillin-Clavulanic Acid, 1 cc/kg, IM) given twice a day for 14 days, with the first 3 days being treated in the Harmoni Pet Care inpatient room and the 4th day to the 14th day of care is done at the owner's house, local antiseptic dressing at the incision site is continued for up to 14 days until the sutures in the skin sutures were absorbed by the cat's body.</em></p> 2022-04-22T00:00:00+00:00 Copyright (c) 2022 Tantri Dyah Whidi Palupi, Tri Wahyu Suprayogi, Ismudiono