Jurnal Medik Veteriner

Ovum Pick-Up and Ovaries Characterization of Black Bengal Goat from Slaughterhouse

Shobhana Nourin — 2024-10-04

Black Bengal goat is the only indigenous breed in Bangladesh. These are small-sized goats renowned for their high-quality meat, skin, and adaptability to adverse environmental conditions. To conserve the originality of this species, it is mandatory to develop an effective methodology for their germ cells to be harvested after death. Therefore, the study was conducted to assess the ovarian status and evaluate the most suitable method of ovum pick-up as well as the characterization of ovum from slaughterhouse specimens. A total of 182 ovaries were collected from local slaughterhouses from June 2022 to July 2023 and grossly examined to assess their status. Two methods were used to retrieve oocytes from the ovaries: aspiration and slicing. The grading of the ovum was done based on the germ layer present surrounding the oocytes. From 182 ovaries, the rates of corpus luteum (CL), tumor, and cyst were found to be 14.29%, 6.59%, and 27.47%, respectively. In the aspiration method, the significantly highest percentage of recovery rate (38.89%) and grade A oocytes (51.06%) were found within 1–3 hours of slaughtering. In the slicing method, the significantly highest percentage of recovery rate (19.57%) and grade A (60.61%) oocytes were found within 1–3 hours of slaughtering. The recovery rate was significantly higher (34.1%) in the aspiration method than in the slicing method (17.1%). There was no significant relationship in oocyte quality between the two methods. The recovery rate was not significantly dependent on the presence of CL. In conclusion, the findings from this study may be useful for assessing the quality of oocytes to preserve the germ cells and utilize them for in vitro fertilization for conserving Black Bengal goat.

Efficacy of Probiotics on Nutrient Intake and Egg Weight in Japanese Quail (Coturnix coturnix japonica)

Amelia Lintang Putri Kirana — 2024-10-04

This study aimed to determine the efficacy of lactic acid bacteria probiotics containing Lactobacillus acidophilus and Lactococcus lactis on nutrient consumption and egg weight of Japanese quail. A total of 120 females Japanese quails comprised four treatments and six replications. T0, T1, T2, and T3 treatment groups were administered L. acidophilus and L. lactis with concentrations of 0, 1, 2, and 3 mL/liter of drinking water. Consumption data were collected weekly, and egg weight data were collected daily during treatment. The data obtained were then analyzed statistically using analysis of variance (ANOVA) and continued with the Duncan multiple range test (p < 0.05). The results of this study showed that probiotics in drinking water reported significant differences (p < 0.05) compared between treatments (T1, T2, and T3) to reduce nutrient intake of organic matter, crude protein, crude fat, crude fiber and increase egg weight of quail. It can be concluded that the combination of L. acidophilus and L. lactis can reduce nutrient consumption but can increase the egg weight of Japanese quail.

Effect of Bone Morphogenetic Protein Receptor-1B (BMPR-1B) Gene Variant on Litter Size in Akkaraman Sheep Breed

Recai Acı — 2024-10-04

The BMPR-1B gene, a significant fertility gene, has been examined for its relationship with fertility characteristics in various sheep populations globally. This investigation explored the impact of the BMPR-1B gene on litter size within the Akkaraman sheep population residing in the Elâzığ Region. To locate the FecB gene in Akkaraman sheep, 104 milk samples were collected, and genetic material was isolated using the salting out technique. During the 2021–2022 period, no significant correlations were observed between the genetic make-up of Akkaraman sheep and factors including age, mastitis and disease history, milk yield, birthing type, and the number of births (p > 0.05). However, there was a noteworthy relationship identified between the distribution of genetic make-up and live weight, indicating a potential influence of genetics on the live weight of Akkaraman sheep (p < 0.05). In this study, the BMPR-1B gene determined multiple births and it was determined that Akkaraman sheep were monomorphic in terms of this gene. In light of this information, it was concluded that more comprehensive studies should be carried out regarding this gene determining multiple births in the Akkaraman sheep breed.

Chloramphenicol Residues and Bacterial Contamination in Farmed African Catfish (Clarias gariepinus) from Banyuwangi Traditional Markets: A Risk Assessment

Mohammad Faizal Ulkhaq — 2024-10-04

This study aimed to determine chloramphenicol residues and bacterial contamination (Aerobic Plate Count-APC, Escherichia coli count, Salmonella sp., and Vibrio cholerae) in farmed African catfish (Clarias gariepinus) that are marketed in Banyuwangi, Indonesia. A total of ninety samples of C. gariepinus were collected from several markets in Banyuwangi, namely, Kertosari, Blambangan, and Banyuwangi Kota. Using standard procedures, APC, E. coli count, Salmonella sp., V. cholerae, and chloramphenicol residues were determined. 36.37% of samples from Kertosari markets; 23.33% of samples from Banyuwangi Kota markets; and 16.67% of samples from Blambangan markets contained chloramphenicol residues, but less than 0.3 ppb. Only 40% of samples from Kertosari markets, 36.67% of samples from Banyuwangi Kota markets, and 26.67% of samples from Blambangan markets were contaminated by E. coli, but were less than 3 MPNg^-1. The highest APC was from Banyuwangi Kota, followed by Kertosari and Blambangan. No samples were contaminated by Salmonella sp. and V. cholerae from any of the markets, and there was no significant difference between all markets in APC, E. coli count, or chloramphenicol residues. All farmed catfish marketed in Banyuwangi were safe to eat because no samples exceeded the maximum chloramphenicol residue and bacterial contaminant standards. Further studies are needed to detect other antibiotic residues used in aquaculture, including tetracycline, sulphonamide, enrofloxacin, and nitrofuran.

Molecular Fish Sexing on Taisho Sanshoku Koi (Cyprinus carpio) Based on ArS.9-15 Gene Amplification using PCR Method

Balqis Bahiya Milan Novindasari — 2024-10-04

Taisho Sanshoku is a variant of Koi fish (Cyprinus carpio) that has high demand due to its high economic value and relatively expensive price. This study aimed to determine the sex of the Taisho Sanshoku Koi fish by molecular sexing using the PCR method to amplify the ArS.9-15 gene. This study was initiated by rearing a 4–6 month-old of 10 Taisho Sanshoku Koi fish in a fish tank with a filter and oxygen aeration. The fish were fed with fish pellets for 1–3 days. The Koi fishes were then anesthetized using Koi anesthesia containing β-hydroxyethyl phenyl ether. Each fish's peripheral blood was collected as much as 0.5 mL per fish and then stored in tubes containing Ca-EDTA anticoagulant. The genomic DNA was extracted from peripheral blood samples and used as a template DNA for PCR amplification targeting ArS.9-15 gene. Agarose with 1.5% concentration and CybrSafe staining was used in electrophoresis for visualization of the PCR results then visualized in a dark chamber using a UV transilluminator. The Taisho Sanshoku Koi fish's sex was determined using descriptive analysis based on the electrophoresis results. According to the PCR results, the female Taisho Sanshoku Koi fish only produced one 800 bp DNA band, whereas the male fish produced two 800 bp and 1,100 bp DNA bands. The outcome of molecular fish sexing of the 10 Taisho Sanshoku Koi fish reported that 60% were male and 40% were female.

Primary and Secondary Sexual Characteristics of Kuhli Loach (Pangio kuhlii)

Darmawan Setia Budi — 2024-10-04

Kuhli loach (Pangio kuhlii) a popular ornamental freshwater fish species with economic potential, originating from Sumatra, Kalimantan, Java, and Malaysia. The kuhli loach's appeal lies in its vibrant colors and distinctive eel-like body. This study aims to explores the sexual primary and secondary characteristics of the kuhli loach. Conducted at Universitas Airlangga in Banyuwangi, Indonesia, the study involved a thorough analysis of 50 kuhli loaches, evenly split between males and females. Morphological observations revealed significant differences (p < 0.05), such as the elongated body and expanded abdominal region in females, suggesting adaptations for reproduction. Internal examinations, including peritoneal dissections and histological analysis, unveiled distinct stages of spermatogenesis in males and ovarian development in females, providing insights into the reproductive cycle. Meristic and morphometric analyses identified noteworthy differences in pectoral fin rays, suggesting potential roles in reproductive behavior, while other traits exhibited similarity between genders. This study contributes to the understanding of kuhli loach ecology and reproductive biology, highlighting the importance of addressing sustainability concerns arising from the continuous exploitation of wild populations. This study serves as a foundational step towards advancing reproductive improvement in fish and supports the conservation of this unique ornamental fish species, with implications for aquaculture practices and economic considerations.

Effect of Stocking Density on Stress-Related Gene Expression of Pacific White Shrimp (Litopenaeus vannamei) Infected with Infectious Myonecrosis Virus (IMNV)

Moch. Nurhudah — 2024-10-04

The stress level of vannamei shrimp (Litopenaeus vannamei) is affected by increased density and several genes are expressed under the condition. This study aimed to determine the expression of genes encoding white shrimp stress after density treatment and the infectious myonecrosis virus (IMNV) challenge test. A completely randomized design (CRD) was carried out with 6 treatment groups, i.e. 3 different stocking density groups without IMNV infection (100, 200, and 400 shrimp/m²) and 3 different stocking density groups + IMNV infection (100, 200, and 400 shrimp/m²). In addition, a shrimp density of 400 shrimp/m² reported the fastest rate of developing the IMNV virus as seen from the clinical symptoms. The lowest cumulative number of shrimp deaths was at a density of 100 shrimp/m² and was caused by the IMNV virus confirmed through RT-PCR. Expression of stress-coding genes was divided into upregulated and downregulated characteristics. The upregulated genes were lectin and translationally controlled tumor protein (TCTP), while the downregulated gene was Toll Receptor. The results showed that the expression of genes related to immunity in L. vannamei was upregulated after pathogen challenges such as lectin and TCTP, meanwhile, the Toll receptor gene was downregulated. Further study should also be performed to measure the expression of the three genes in revealing the immune pathways.

Primordial Germ Cells Quality of Chicken Embryo from Post Cryopreservation Blood Samples

Nurul Azizah — 2024-10-04

The optimal cellular reservoir for the conservation and propagation of endangered poultry is acknowledged to be primordial germ cells (PGCs). This study aimed to evaluate and compare the efficacy of cryopreserving PGCs derived from purified sources and blood samples. The experimental design encompassed two treatment groups, each iterated six replication. Blood samples that had been filled with 500 µL of phosphate-buffered saline without Ca²⁺ and Mg²⁺ (PBS) underwent centrifugation at 1200 rpm for six minutes. The supernatant was discarded and the pellet was mixed with 500 µL 10% FBS-PBS and was then subdivided for cryopreservation and PGCs purification. Cryopreservation included the controlled freezing of blood and purified PGCs samples in Nalgene cryogenic vials with the incorporation of banker cells. Following thawing, statistical analyses revealed no significant variance in the mean PGCs count between purified PGCs and blood samples (p > 0.05). The percentages of PGCs mortality and the recovery rate demonstrated statistical significance (p < 0.05). Further investigations indicated that cryopreserving blood samples significantly increased the recovery rate of PGCs while decreasing post-thaw mortality. Morphology of PGCs from blood cryopreservation showed similar morphology to PGCs in general. The potential application of cell-based cryo-banking will contribute to the preservation of poultry genetic diversity for sustainability and adaptation to future poultry demands.

Macroanatomy, Histomorphometry, and Androgen Receptor Expression in the Epididymis of Kacang Goats Aged 4, 8, and 12 Months

Herlina Pratiwi — 2024-10-04

The epididymis is a crucial component of the goat's reproductive structure. The epididymis is responsible for the transportation, concentration, and maturation of sperm. This study aimed to examine the differences in the structure of macro and microanatomy, tissue composition, and the existence of androgen receptors in the epididymis of Kacang goats aged 4, 8, and 12 months. The assessment of macroscopic organ growth was done immediately after sampling, whereas microscopic measurements were carried out following histological preparations using hematoxylin-eosin (HE) and immunohistochemical (IHC) procedures. The results on the macroscopic anatomy of the epididymis indicated a significant association between age with the width of the caput dexter and sinister and the circumference of the right cauda. However, no significant relationship was found between age with the corpus length and the left cauda's circumference. Significant variations were observed in the diameter and concentration of the agglutinated spermatozoa in the lumen during histomorphometry of the epididymis in three age groups of Kacang goats. There were no statistically significant variations in the expression of androgen receptors among the three age groups. This study showed that the correlation coefficient test reveals a positive relationship between age and the caput width and corpus length dimensions, indicating that these measurements tend to grow as age increases. On the other hand, the diameter of the agglutinated spermatozoa in the epididymal lumen exhibits significant variations between the ages of 4 months, with the ages of 8 months and 12 months, suggesting that the sperm becomes fully matured by the age of 8 months.

Development of Skeletal Ossification in Climbing Perch (Anabas testudineus) from Juvenile to Adulthood

Tri Wahyu Pangestiningsih — 2024-10-04

The climbing perch (Anabas testudineus) is a freshwater fish that can withstand highly unfavorable environments and stay out of the water for extended periods. Its anatomical characteristics showed terrestrial adaptation and terrestrial locomotion properties enable the use of climbing perch as an animal model. Moreover, its bone and cartilage profiles at different stages are crucial to improving the anatomical information for the osteogenesis model candidate. The current study aimed to illustrate the skeletal profiles of juvenile and adult climbing perch with the whole staining method. The samples included two adults and two juvenile climbing perch from Daerah Istimewa Yogyakarta, Indonesia. The fish were euthanized using β-hydroxyethyl phenyl ether in a lethal dose. The skeleton was examined using the Alcian blue–Alizarin red whole staining, which revealed the bones and cartilage under the stereomicroscope. The result showed that the bone is the main skeleton. Cartilage was detected in the area surrounding the orbit in the adult climbing perch and in the pterotic, pterosphenoid, prootic, and distal end of the hypural, parhypural, and basal pectoral girdle in the juvenile climbing perch. Endochondral osteogenesis was found in juvenile to adult climbing perch.

Detection of the CTX-M Gene Associated with Extended-Spectrum β-Lactamase (ESBL) in Broiler Chickens in Surabaya Traditional Markets

Mariana Febrilianti Resilinda Putri — 2024-10-04

A common indicator used to examine the frequency and distribution of antibiotic resistance against other enteric bacteria in humans and animals is the commensal enteric bacterium, Escherichia coli. The transmission of plasmids harboring ESBL enzymes, primarily generated by E. coli, is the cause of this resistance. The purpose of this study was to identify the CTX-M gene in ESBL-producing E. coli from broiler chicken cloacal swabs in traditional Surabaya markets. The samples used were 96 cloacal swabs from broiler chickens in the traditional markets of Dukuh Kupang, Keputran, Pacar Keling, and Pucang. The antibiotic disks used in this study belonged to five different antibiotic classes; they are aztreonam (monobactam), chloramphenicol (phenicol), kanamycin (aminoglycoside), ciprofloxacin (fluoroquinolone), and tetracycline (tetracycline). Presumptive ESBL strains were then molecularly screened for the presence of CTX-M gene. Results revealed that out of the 96 chicken cloacal swab samples collected, 58 (60.42%) were positive for E. coli based on morphological culture, Gram staining, and biochemical tests. Additionally, 15 out of the 58 E. coli isolates recovered from broiler chicken cloacal swabs were multidrug-resistant (MDR) while 7 of E. coli isolates harbored CTX-M gene. Conclusively, this study has shown that broiler chickens sold in traditional Surabaya markets harbor MDR E. coli which possess CTX-M gene. Conditions in traditional markets with low levels of cleanliness and chickens placed close together can spread resistance genes with serious public health consequences. Therefore, it is imperative to observe good hygienic practices in Surabaya traditional markets in order to curtail the spread of MDR bacterial pathogens in the food chain.

Phylogenetic and Genetic Variation Analysis of ITS1 Gene of Trypanosoma lewisi in Wild Rats Using Polymerase Chain Reaction

Reza Yesica — 2024-10-04

Murine Trypanosomiasis is a disease caused by the blood protozoan Trypanosoma lewisi in rats, with the transmission process mediated by the flea species Xenopsylla cheopis and Nosopsyllus fasciatus. Cases of trypanosomiasis have been documented due to Trypanosoma lewisi infecting rats and humans in various countries. Diagnosis of T. lewisi is typically conducted using polymerase chain reaction (PCR), which amplifies target DNA using specific primers. One such target gene for detection is the Internal Transcribed Spacer-1 (ITS1). Subsequent sequencing of PCR products enables analysis of genetic variation employing parameters such as nucleotide composition, genetic distance, and phylogenetic analysis with MEGA software. Test results based on percent identity values indicated a 98.51% homology of blood samples with the Chinese strain of T. lewisi (FJ011094.1), demonstrating genetic variation. Phylogram reconstruction revealed that samples 18, 19, and 37 of T. lewisi exhibit very close intraspecies relationships with T. lewisi from NCBI genebank with genetic distance ranging from 0.007 to 0.01. While the closest interspecies relationship was found with T. cruzi (KT305857.1) with a genetic distance of (d = 0.61).

Prevalence of Gastrointestinal Parasites in Pigs in Bali

Ayu Komang Ria Trie Dewi Pinatih — 2024-10-04

This study aimed to identify gastrointestinal parasites in pigs in Bali. A total of 117 pig feces samples were collected in Buleleng Regency (n = 67) and Jembrana (n = 50). Samples were examined microscopically using native, sedimentation, and floating methods. The results reported the prevalence of gastrointestinal parasites infecting pigs in Bali was 94.8% (111/117) infected with protozoa, namely Eimeria sp. (90.5%), Entamoeba sp. (26.4%), Isospora suis (6.8%), and Balantidium sp. (5.1%), while 99.1% (116/117) were infected with helminths, namely Trichuris suis (71.7%), Strongyloides sp. (64.9%), Ascaris suum (49.5%), Oesophagostomum sp. (6.1%), Macracanthorhyncus sp. (2.5%), and Hyostrongylus sp. (0.8%). Based on the tree regression analysis reported that the rearing system was related to the degree of gastrointestinal parasite infection in pigs in Bali.

Study of Animal Owners’ Knowledge of Helminthiasis in Cats at Mayda Animal Care and iVet Clinic Dramaga, Bogor

Muhammad Baharudin Yusuf — 2024-10-04

Animal owners often overlook helminthiasis because it does not cause specific clinical symptoms in mild infections. This study aimed to analyze the medical records of helminthiasis in cats and determine the knowledge of animal owners about helminthiasis through questionnaires at Mayda Animal Care and iVet Clinic Dramaga, Bogor. A cross-sectional study was used to analyze medical record data and then compared using descriptive methods with a secondary data approach. Data analysis was performed using the Chi-square test. The findings of helminthiasis cases in cats at Mayda Animal Care during January–December 2022 amounted to 102 cases, while at iVet Clinic Dramaga, there were 50 cases. Based on the Chi-square test, this study reported a significant correlation (p < 0.05) between age and the level of action and education with the level of understanding of animal owners on helminthiasis at Mayda Animal Care and iVet Clinic Dramaga, Bogor.

Strategic Infectious Diseases in Beef Cattle in Balongpanggang during 2023

Wiwik Misaco Yuniarti — 2024-10-04

Strategic infectious animal diseases have a great impact on the national economy as they may cause great economic loss, public anxiety, and high mortality. In Indonesia, among the diseases considered as strategic infectious animal diseases is foot and mouth disease (FMD). This study reported the incidence of strategic infectious animal diseases in Balongpanggang district during 2023. Throughout 2023, FMD only accounted for 4% of the total diseases of the year and was the only strategic infectious animal disease reported that year. Meanwhile, bovine ephemeral fever (BEF) incidence reached 57% (876/1546) of the total diseases in beef cattle in 2023. In addition to BEF, official veterinary services reported a total case of 1546 throughout 2023 that comprises; diarrhea (99), limping (24), helminthiasis (91), ectoparasite (11), hematochezia (3), wound (3), mastitis (4), allergy (1), dystocia (28), vaginal prolapse (11), dermatitis (45), enteritis (27), tympani (11), scabiosis (122), arthritis (67), ophthalmic problem (11), endometritis (1), uterine retention (13), indigestion (2), pneumonia (21), hypoglycemia (5), abscess (21), ovariohysterectomy (1), orchitis (1), intoxication (1), and papilloma (1). In conclusion, the only strategic infectious animal disease reported in 2023 in Balongpanggang was FMD with the incidence as high as 3% as compared to the non-strategic infectious animal diseases, BEF, which reached 54% of the total cases during 2023.

Prevalence of Ectoparasites in Tilapia (Oreochromis niloticus) in Sidoarjo, Indonesia

Aulia Puspita Sari — 2024-10-04

The problem most often encountered in fish farming is disease which can cause a decrease in fish production levels. Parasites cause one of the infectious diseases found in fish farming. The presence of parasites can cause a decrease in the quality of the growth of fish production. The purpose of this study was to identify the prevalence of ectoparasites on Nile tilapia in Sidoarjo, Indonesia. Ninety-six samples from two ponds were examined under the microscope to find out the presence of ectoparasite that exists in the outside body part of the fish. Samples were collected from skin, fins, tail, and gills using native methods and observed under a microscope in the Division of Parasitology Laboratory, Faculty of Veterinary Medicine, Universitas Airlangga. The results of this study showed 86 samples positively infected by ectoparasite and 10 showed negative. The ectoparasites found in this study were Trichodina sp., Chilodonella sp., Ichthyophthirius multifillis, Gyrodactylus sp., and Dactylogyrus sp. The highest prevalence is Trichodina sp. and Dactylogyrus sp. The number of fish infested by ectoparasite is 89.56%, this is classified in the usual category. The highest prevalence occurs in infestations of Trichodina sp. i.e.72.91% which were contained in the 70 samples. While the lowest prevalence was in Gyrodactylus sp. by 8.32% in 8 fish samples. Double infections from two different ponds were 70% within 33 samples which were included in the usual category.

Effect of Traditional Chinese Medicine on Fish Growth: A Meta-Analysis Study

Salsabila Salsabila — 2024-10-04

Fish productivity has long been the subject of studies using traditional Chinese medicine (TCM). To validate TCM's effectiveness on weight gain rate (WGR) and specific growth rate (SGR), a meta-analysis study was conducted. Of the 58 studies collected from PubMed, Scopus, and Cochrane Library, 4 eligible studies were analyzed quantitatively. The extracted data were then analyzed using Review Manager (RevMan) online to determine the standard mean difference (SMD), random effect, heterogeneity, and 95% confidence intervals (95% CI). As a result, TCM administration had a favorable effect on WGR (SMD = 8.33; p = 0.0001) and SGR (SMD = 8.12; p = 0.0004). In conclusion, this meta-analysis study emphasized the efficacy of TCM to improve fish production performance.

Optimization of Poultry Physiological Condition in the Post-Antibiotic Era through Nutritional Intervention – A Review

Sugiharto Sugiharto — 2024-10-04

The intestinal microbe population has a considerable impact on the physiological state of poultry. Nutritional intervention can be used to enhance the equilibrium of the gut microbiota in chickens, leading to good physiological effects. Various nutritional interventions have been implemented, including feed additives, vitamins, and fermented and functional feeds. While numerous studies have shown the efficacy of nutritional strategies, others have noted the variability of these interventions on poultry’s physiological state. Various factors can influence the effectiveness of nutritional interventions on the physiological conditions of poultry, such as the types of feed additives or active components, duration and method of administration, feed composition, environmental conditions during rearing, quality of day-old-chick, type and age of poultry, hygiene, infection, stress, and other related variables. Feed additives, when combined with nutritional components or other active chemicals, can have synergistic and complementary effects. These impacts could improve the effectiveness and reliability of the nutritional interventions on physiological parameters and poultry productivity. Furthermore, combinations can include feed additives or active components like probiotics and acidifiers, probiotics and enzymes, phytobiotics and enzymes, and plant-based materials and chitosan. The combinations show synergistic and complementary benefits, improving physiological conditions in chickens more than using feed additives or active substances alone. Overall, nutritional intervention can be used to improve the microbial balance in poultry intestines and boost their physiological state. Moreover, integrating feed additives with other active components might enhance the physiological circumstances of chickens due to the synergistic effect produced by their combination.

Immune Responses of Club Cells in Fish: A Review

Yeni Dhamayanti — 2024-10-04

The primary line of defense against pathogens from the environment is often fish epidermis tissue. Nevertheless, little is understood about the physiological mechanisms that underlie the non-specific and/or specific protection that these cells can offer. The exact nature of the relationship between the evolution of ostariophysan fish club cells and chemical warning signals is still unknown and controversial. Fish epidermis layer cells comprise mucus cells, lymphocytes, macrophage cells, cuboidal and squamous epithelial cells, and cells specific to certain fish species. Club cells, also called "alarm cells," are chemical alarms that sound in the event of a potentially hazardous scenario. These cells will burst in the presence of a predator, releasing pheromones that, if the skin is physically damaged, trigger an avoidance and terror reaction. In sturgeon larvae, mucus cells were visible in week 1, but club cells did not appear until week 4. Due to their later development during ontogenesis and after wounding, club cells may not have as much of a protective effect during wound healing as filament or mucus cells. Club cells are mostly found in the epidermis of the skin, and it is thought that when they work in tandem with mucus and goblet cells to fight infections, they serve as the body's first line of defense.

Front Cover, Editorial Board, Peer Reviewers, Acknowledgments

Choirun Nisa, SIIP. — 2024-10-09

Indexing, Subscribed Form, Guidelines for Author, Back Cover

Choirun Nisa, SIIP. — 2024-10-09

Sebaceous Adenoma in a Geriatric Poodle Dog: A Case Report

Ida Bagus Oka Winaya — 2024-10-04

A 14-year-old black male Poodle was brought to the Animal Teaching Hospital, Udayana University by its owner with clinical signs of frequent licking of its left front paw. Upon examination, red bumps were observed on the left front leg, accompanied by small, round black spots scattered on the dorsal side of the body. Additionally, black nodules were present on the lower eyelids and hind limbs. Surgical intervention was undertaken to excise the tumor mass, with the animal under anesthesia induced by ketamine at 5 mg/kg BW intravenously. The reddish nodule was excised by performing an elliptical incision at the base of tumor. Postoperatively, the animal received an antibacterial injection comprising ceftriaxone and tazobactam at 25 mg/kg BW intramuscularly and antiseptic wound dressing for supportive care. Microscopic examination revealed neoplastic cells arranged into lobules of varying sizes and shapes within the tumor mass. These lobules consisted of differentiated sebocytes and basaloid cells. At the periphery of the neoplastic lobules, the basaloid cells displayed several layers and exhibited invasion with moderate anisocytosis. The mitotic index was no more than ten cells in one field of view. Based on these histopathological features, the tumor was confirmed to be a sebaceous adenoma. After a 10-month follow-up period, there were no signs of tumor recurrence observed.