The Effectiveness of Lactobacillus rhamnosus Administration on the Growth of Klebsiella pneumoniae in Mice (Mus musculus)
Downloads
Introduction: Klebsiella pneumoniae is a bacterium from the Enterobacteriaceae family and is considered one of the most dangerous pathogens. Antibiotics are crucial for treating acute bacterial infections. Using probiotics has become one of the supportive and curative efforts in managing the growth of K. pneumoniae. One probiotic derived from microorganisms is Lactobacillus rhamnosus. This study aimed to determine the effectiveness of L. rhamnosus on the growth of K. pneumoniae in mice (Mus musculus).
Methods: This was an experimental post-test study. The study population consisted of mice aged 3 to 4 months, weighing 20 to 40 grams, determined using Federer’s formula. Mice were given standard feed and sterile distilled water. A total of 30 mice were tested and divided into five treatment groups. The distribution colony count test was used for evaluation.
Results: Among the five groups studied, probiotic intervention in the group receiving a combination of L. rhamnosus and the antibiotic ceftriaxone showed effective results (p<0.05), as did the group given only the probiotic L. rhamnosus (p<0.05). In contrast, the other groups did not show effective results (p>0.05).
Conclusion: The administration of L. rhamnosus is effective as a supportive and curative treatment, but it is not effective as a preventive measure against the growth of K. pneumoniae in mice.
Effah CY, Sun T, Liu S, et al. Klebsiella pneumoniae: An Increasing Threat to Public Health. Ann Clin Microbiol Antimicrob 2020; 19: 1. [PubMed]
El-Mokhtar MA, Hassanein KM, Ahmed AS, et al. Antagonistic Activities of Cell-Free Supernatants of Lactobacilli against Extended-Spectrum β-Lactamase Producing Klebsiella pneumoniae and Pseudomonas aeruginosa. Infect Drug Resist 2020; 13: 543–552. [PubMed]
Sunarno S, Puspandari N, Fitriana F, et al. Extended Spectrum Beta Lactamase (ESBL)-Producing Escherichia coli and Klebsiella pneumoniae in Indonesia and South East Asian countries: GLASS Data 2018. AIMS Microbiol 2023; 9: 218–227. [PubMed]
World Health Organization (WHO). Global Antimicrobial Resistance and Use Surveillance System (GLASS) Report. Geneva, https://iris.who.int/bitstream/handle/10665/341666/9789240027336-eng.pdf (2021).
Al-Sheboul SA, Al-Madi GS, Brown B, et al. Prevalence of Extended-Spectrum β-Lactamases in Multidrug-Resistant Klebsiella pneumoniae Isolates in Jordanian Hospitals. J Epidemiol Glob Health 2023; 13: 180–190. [Springer]
Hermawan A. The Environmental Factors and Sociodemographic Characteristics of Pneumonia Incidence in Indonesia. J Aisyah J Ilmu Kesehat 2022; 7: 1155–1164. [Journal]
Waworuntu O, Sjahril R, Rasita YD, et al. Characteristic of Extended-Spectrum-B-Lactamase (ESBL) Producing Klebsiella pneumoniae at Tertiary Referral Hospital in South Sulawesi, Indonesia. Int J Infect Dis 2020; 101: 80. [ScienceDirect]
Tang HJ, Chen CC, Lu YC, et al. The Effect of Lactobacillus with Prebiotics on KPC-2-Producing Klebsiella pneumoniae. Front Microbiol 2022; 13: 1050247. [PubMed]
Yamasaki S, Shigemura K, Osawa K, et al. Genetic Analysis of ESBL-Producing Klebsiella pneumoniae Isolated from UTI Patients in Indonesia. J Infect Chemother 2021; 27: 55–61. [ScienceDirect]
Savinova OS, Glazunova OA, Moiseenko KV, et al. Exoproteome Analysis of Antagonistic Interactions between the Probiotic Bacteria Limosilactobacillus reuteri LR1 and Lacticaseibacillus rhamnosus F and Multidrug Resistant Strain of Klebsiella pneumonia. International Journal of Molecular Sciences; 22. Epub ahead of print 2021. [Journal]
Chen CC, Lai CC, Huang HL, et al. Antimicrobial Ability and Mechanism Analysis of Lactobacillus Species against Carbapenemase-Producing Enterobacteriaceae. J Microbiol Immunol Infect 2021; 54: 447–456. [ScienceDirect]
Silva DR, Sardi JDCO, Pitangui NDS, et al. Probiotics as an Alternative Antimicrobial Therapy: Current Reality and Future Directions. J Funct Foods 2020; 73: 104080. [ScienceDirect]
Albarracin L, Maidana SD, Fukuyama K, et al. Orally Administered Lactobacilli Strains Modulate Alveolar Macrophages and Improve Protection against Respiratory Superinfection. Biomolecules; 14. Epub ahead of print 2024. [Journal]
Steele C. Lactobacillus rhamnosus GG : A Review of Clinical Use and Efficacy. Nutr Med J 2022; 1: 70–116. [Journal]
Penchuk Y, Savytska M, Kobyliak N, et al. Antimicrobial Activity of Dietary Supplements Based on Bacterial Lysate of Lactobacillus rhamnosus DV. Front Cell Infect Microbiol 2023; 13: 1211952. [PubMed]
Maidana SD, Imamura Y, Elean M, et al. Oral Administration of Lacticaseibacillus rhamnosus CRL1505 Modulates Lung Innate Immune Response against Klebsiella pneumoniae ST25. Microorganisms; 11. Epub ahead of print April 2023. [PubMed]
Klimina KM, Dyachkova MS, Veselovsky VA, et al. Transcriptional Responses of Lacticaseibacillus rhamnosus to TNFα, IL-6, IL-8, and IL-10 Cytokines. Biology; 13. Epub ahead of print 2024. [Journal]
Huang R, Wu F, Zhou Q, et al. Lactobacillus and Intestinal Diseases: Mechanisms of Action and Clinical Applications. Microbiol Res 2022; 260: 127019. [ScienceDirect]
Riwu KHP, Effendi MH, Rantam FA, et al. A Review: Virulence Factors of Klebsiella pneumonia as Emerging Infection on the Food Chain. Vet World 2022; 15: 2172–2179. [PubMed]
Abdelhalim MM, Saafan GS, El-Sayed HS, et al. In Vitro Antibacterial Effect of Probiotics against Carbapenamase-Producing Multidrug-Resistant Klebsiella pneumoniae Clinical Isolates, Cairo, Egypt. J Egypt Public Health Assoc 2022; 97: 19. [Journal]
Kheiri F, Kermanshahi RK, Feizabadi MM. The Inhibitory Effects of Lactobacillus Supernatants and Their Metabolites on the Growth and Biofilm Formation of Klebsiella pneumoniae. Infect Disord Drug Targets 2020; 20: 902–912. [PubMed]
Zhu J, Wang T, Chen L, et al. Virulence Factors in Hypervirulent Klebsiella pneumoniae. Front Microbiol 2021; 12: 642484. [PubMed]
Yang H, Sun Y, Cai R, et al. The Impact of Dietary Fiber and Probiotics in Infectious Diseases. Microb Pathog 2020; 140: 103931. [ScienceDirect]
Copyright (c) 2025 Izhar Fitrah Ahmad, Edward Pandu Wiriansya, Dwi Anggita, Rezky Pratiwi Lambang Basri, Yani Sodiqah, Nesyana Nurmadilla
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
1. The journal allows the author to hold the copyright of the article without restrictions.
2. The journal allows the author(s) to retain publishing rights without restrictions.
3. The legal formal aspect of journal publication accessibility refers to Creative Commons Attribution Share-Alike (CC BY-SA).
4. The Creative Commons Attribution Share-Alike (CC BY-SA) license allows re-distribution and re-use of a licensed work on the conditions that the creator is appropriately credited and that any derivative work is made available under "the same, similar or a compatible license”. Other than the conditions mentioned above, the editorial board is not responsible for copyright violation.
JR (