The Role of adaptive MSCs as an Attempt Regeneration of Spermatogenesis Process Using by Hypoxia Precondition In Vitro

erma safitri

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The aim of this research was to obtain to get adaptive MSCs by an treatment in hypoxia precondition in viro culture. Ini this research, hypoxia precondition was to be given 3% O2 concentration and compared to those of normoxia culture in O2 > 20%. Results showed that under 3% O2 concentration, CD105+ and CD45- by flowcytometri, immunocytokimia and immunofluorescence didn’t experience of change (undifferentiated). Meanwhile under > 20% O2 concentration, cells have experienced of change (not undifferentiated again), that was indicated with CD105+ become decrease and CD45- increase by flowcytometri, immunocytokimia and immunofluorescence. Conclusion, in this research showed that hypoxia precondition with 3% O2 concentration very support MSCs to constantly adaptive before transplantated for disturbance of spermatogenesis process, because did’nt experience become progenitor cells was not expectation (still undifferentiated).


MSCs, hypoxia precondition 3% O2 concentration, adaptive.

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Blanchard K.T., J. Lee, and K. Boekelheide. 1998. Leuprolide, a Gonadotropin- Releasing Hormone Agonist, Reestablishes Spermatogenesis After 2,5- Hexanedione-Induced Irreversible Tsticular Injury in the Rat, Resuting in Normalized Stem cell Factor Expression. Endocriology.139 (1): 236-244.

Cavallini G. 2006. Male idiopathic oligoasthenoteratozoospermia. Asian J. Androl., 8(2): 143-157.

Hamanaka RB and NS Chandel. 2010. Mitochondrial Reactive Oxygen Species Regulate Cellular Signaling and Dictate Biological Outcomes Trends Biochem. Sci., 35: 505-513.

Kenichiro H., N. Noritoshi, I. Takashi, T. Itoh., S. Murakami, Y. Shimizu,W. Taki, K. Miyatake,K. Kangawa. 2005. Adrenomedullin Enhances Therapeutic Potency of Mesenchymal Stem Cells after Experimental Stroke in Rats. Stroke36: 853-858.

Rantam FA, Ferdiansyah, Nasronudin and Purwati. 2009. Stem Cell Exploration- Methods of lsolation and Culture First Ed. Airlangga University Press. Surabaya.

Suda T, Takubo Kand Semenza GL. 2011. Cell Stem Cell Review. Metabolic Regulation of ematopoietic Stem Cells in Hypoxic Niche. Cell Stem Cell., 9: 296-310.

Suzuki K, Murtuza B and Beauchamp JR. 2004. Dynamics and Mediators of Acute Graft Attrition After Myoblast Transplantation to the Heart. FASEBJ., 18: 1153-1155.

Takubo K, Goda N, Yamada W, Iriuchishima H, Ikeda E, Kubota Y, Haruko S, Jhonson RS, Hirao A, SuematsuM and Suda T.2010. Regulation of the HIF-1 Level is Essentialfor Hematopoietic Stem Cells. Cell Stem Cell., 7: 391-402.

Tang Y.L., Y.C. Zhang, L.P. Qian, Shen, and M. Phillips (2005). Improved Graft Mesenchymal Stem Cell Survival in Ischemic Heart with a Hypoxia regulated Heoxygenase 1 Vector. J. Am. Coll. Cardiol.46: 1339-1350.


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