Rimbun Rimbun, Viskasari Pintoko Kalanjati

= http://dx.doi.org/10.20473/mbiom.v25i2.2012.33-40
Abstract views = 371 times | downloads = 438 times


Neurological disorders become a significant and growing problems. Both clinical and preclinical researchers conducted experiments in human tissue and animal model. The preparation of animal’s tissue needs the right staining methode to show the wanted cells or structures clearly. The nervous tissue contains neurons and neuroglial cells (astrocytes, oligodendrocytes, microglia, and ependyma). The routine staining such as Haematoxyllin and Eosin, more clearly shows the nucleus and organells within the cytoplasm of neurons and neuroglias, but less clearly shows their specific morphology. The heavy metal impregnation techniques, such as silver staining, gold staining, Golgi method, Cajal method, and Rio Hortega method, can overcome this problem and clearly show the morphology of neuron and neuroglia also the neuroanatomical connections in the brain. Other alternative stainings for nervous tissue are Nissl staining, tolluidin blue, methylene blue and Luxol fast blue. There are also immunohystochemical staining methods, i.e. antibody against glial fibrillary acidic protein (GFAP) for astrocyte, monoclonal antibody againts human leucocyte antigen (HLA) for microglia, etc. Based on the variation of staining methods in the nervous tissue, researchers must have a good plan to carefully determine which staining method should be used.


Nervous system; histological preparation; staining method; Sistem saraf; preparat histologi; metode pewarnaan

Full Text:



Bentivoglio, M. et al., 2010. Camillo Golgi and modern neuroscience. J Brain Research Reviews, 66, hal.1-4.

Carlos, J.A.D. & Borrell, J., 2007. A historical reflection of the contributions of Cajal and Golgi to the foundation of neuroscience. J Brain Research Reviews, 55, hal.8-16.

Crossman, A.R. & Neary, D., 2010. Neuro-anatomy: an illustrated color text. Edisi ke-4. London: Churchil Livingstone Elsevier, hal.1-32.

Geneser, F., 2007. Atlas berwarna histologi. Diterjemahkan oleh J. Tambayong. Batam: Binarupa Aksara, hal.56-65.

Hirtz, D. et al., 2007. How common are the “common” neurologic disorders? Neurology, 68(5), hal.326-37.

Hicks, A. Schallert, T. & Jolkkonen, J., 2009. Cell-based therapies and functional outcome in experimental stroke. J Cell Stem Cell 5, hal.139-140.

Kessel, R.G., 1998. Basic medical histology. New York: Oxford University Press, hal.249-275.

Marin, V.G. Lopez, P.G. & Freire, M., 2007. Cajal’s contributions to glia research. J Trends in Neuroscience, 30(9), hal.379-487.

Mescher, A.L., 2010. Junqueira's Basic Histology: a text and atlas. Edisi ke-12. USA: McGraw-Hill Companies.

Ranjan, A. & Mallick, B.N., 2012. Differential staining of glia and neurons by modified Golgi-Cox method. J Neuroscience Methods, hal.1-11.

Ross, M.H. & Pawlina, W., 2011. Histology a text and atlas. Edisi ke-6. Philadelphia: Lippincott Williams & Wilkins, hal.352-390.

Saper, C.B. & Maunsell, J.H.R., 2009. The Neuroscience Peer Review Consortium. J Brain Research, 1272, hal.1-2.

Dua, T. et al., 2006. Public health principles and neurological disorders. In: World Health Organization. Neurological disorders: public health challenges. Geneva: WHO Press. Ch.1.

Young, B. & Heath, J.W., 2000. Wheather’s functional histology. Edisi ke-4. London: Churchil Livingstone Elsevier, hal.116-142.


  • There are currently no refbacks.

Copyright (c) 2019 Majalah Biomorfologi

Creative Commons License
Majalah Biomorfologi (p-ISSN:0215-8833; e-ISSN:2716-0920) is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

View Majalah Biomorfologi Statistics


 Indexed By