Arni Kusuma Dewi, Iskantijah Budi Rahardjo, Ari Gunawan

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Diabetes mellitus complication that much happen and need attention is infection. Bacterial infection getting worse with the decrease of cellular immunity diabetic patient. Antibiotic treatment has not been optimaly yet without immunity intervention to the patient. This study is done to evaluate immunity state in diabetes with count lymphocyte in PALS spleen then compare with healthy ones. Thirty six mice BALB/c strain, sex male ware separated into 2 groups, the control group and the treatment group. The control groups were healty and had standart diet, while the treatment groups were diabetic group and had high fat diet to induct diabetes for ten weeks. After 10 weeks, both groups were induced with histamine intraperitoneally to show immune respons. Mice were sacrified for taken the spleen to make histology preparation with routine staining. Account of lymphocyte both groups compared with statistical tools by t test independent samples. Account of lymphocyte cell in the PALS area shown that the control had significant more than the treatment (p<0,0001). Smaller number of lymphocyte in diabetic is one of the proofs that there are decrease of immunity.


Diabetes mellitus; BALB/c mice; lymphocyte of PALS spleen; mencit balb/c; limfosit pada PALS limpa

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