Oxygen Hyperbaric Exposure Induces GLUT4 Expression Reduction and No Folliculogenesis Alterations in Rat PCOS with Insulin Resistance Model

Varianida Veterini, Budi Santoso, Widjiati Widjiati

= http://dx.doi.org/10.20473/mog.V23I32015.112-117
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Abstract


 

Objectives: To know effect of hyperbaric oxygen therapy to GLUT4 expression and folliculogenesis in rat PCOS with insulin resistent model.

Materials and Methods: this is an analytic experimental study used 30 rats induced by testosterone propionate injection 1 mg/100 g body weight daily for 28 consecutive days as a PCOS with insulin resistent model. Animal models divided into 3 groups. Negative control was not given treatment, positive control was given placebo. Treatment group was given hyperbaric oxygen 2.4 ATA 90 minutes 2x5 days. GLUT4 expression determined by immunohystochemistry to m. Gastrocnemius. Hematoxylin Eosin staining to rat ovaries were performed to know differentiation in folliculogenesis. The results were then compared.

Results: There were significant decrease of GLUT4 expression in treatment group (mean0,84+0,47)compared to negative and positive control (mean3,96+3,16and3,36+2,17). There were no different folliculogenesis in these groups.

Conclusion: Hyperbaric oxygen 2.4 ATA 90 minutes 2x5 days induce decrease of GLUT4 expression and no alterations in folliculogenesis in rat PCOS with insulin resistent model, therefore can not yet be used as alternative therapy in PCOS.

 

 


Keywords


hyperbaric oxygen, PCOS, insulin resistent, GLUT4, folliculogenesis.

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References


Goodarzi MO, Dumesic DA, Chazenbalk G and Azziz R. Polycystic Ovary Syndrome: Etiology, Pathogenesis and Diagnosis. Nature Reviews, Endocrinology. 2011.7. p : 219-231.

Strauss III JF, Dunaif A. Molecular mysteries on polycystic ovary syndrome. Mini review. Molecular Endocrinology. 2013;13(6):800-5.

Yarali H, Yildiz BO, Demirol A, Zeyneloglu H, Yigit N, Bukulmez O, Koray Z. Co-administration of metformin during rFSH treatment in patients with clomiphene citrate-resistant polycystic ovarian syndrome: a prospective randomized trial. Human Reproduction. 2002;17(2):289-94.

Wilkinson D, Hellbron K, Chapman I. Short report: Patophysiology hyperbaric oxygen therapy improves peripheral insulin sensitivity in humans. Diabetic medicine. 2012:986-9.

Al-Waili N, Butler G, Beale J, Abdullah M, Finkelstein M, Merrow M, et al. Influences of hyperbaric oxygen on blood pressure, heart rate, and blood glucose levels in patients with diabetes mellitus and hypertension. Archives of medical research. 2006;37:991-7.

Harnanik T. Efek oksigenasi hiperbarik terhadap peningkatan aktivitas antioksidan pada penderita Diabetes Mellitus tipe 2. Program Pascasarjana Universitas Airlangga. 2008.

Huang S, Czech M. The GLUT4 glucose transport-er. Cell Metabolism. 2007:247-52.

Thom SR. Oxidative stress is fundamental to hyperbaric oxygen therapy. J Appl Physiol. 2009;106:988-95.

Leney SE and Tavare JM. The molecular basis of insulin-stimulated glucose upatake: signalling, trafficking and potential drug targets. Journal of endocrinology. 2009:1-18.

Lira VA, Soltow QA, Long JH, Betters JL, Sellman JE and Criswell DS. Nitric oxyde increases GLUT4 expression and regulates AMPK signalling in skeletal muscle. Am J Physiol Endocrinol Metab. 2007;293:1062-7.

Fritz MA and Speroff L. Chronic Anovulation and the Polycystic Ovary Syndrome. In Cinical Gynecologic Endocrinology and Infertility. 8th Eds. Philadelphia: Lippincott Williams & Wilkin:2011. p. 495-532.

Novak M, Madej J and Dziegeil P. Intensity of Cox 2 expression in cell of soft tissue fibrosarcomas in dog as related to grade of tumor malignation. Bull Vet inst Pulawy. 2007;51: 275-9.

Najah, Al-Mousawi, Al-Terahi AM, Mohammad BI and Hassan ES. Effect of sodium valproate on selected reproductive hormone, lipid profiles, and ovarian histology in female rats. Medical Journal of Basrah University. 2008:57-64.

Ciaraldi T, El-Roeiy A, Madar Z, Reichart D, Olefsky J, Yen S. Cellular mechanisms of insulin resistance in polycystic ovarian syndrome. Journal of Clinical Endocrinology and Metabolism. 1992; 75(2):577-83.

Dunaif A, Xia J, Book C, Schenker E, Tang Z. Excessive insulin receptor serine phosphorylation in cultured fibroblasts and in skeletal muscle. J Clin Invest. 1995;96:801-10.

Salehi RV, Alvera and Poretsky. Pathogenesis of polycystic ovary syndrome. J Metab. 2004:358-76.

Santoso B. Mekanisme penebalan dinding folikel pada sindroma ovarium polikistik. Program Pasca-sarjana Universitas Airlangga Surabaya. 2009.

Muttaqin DA. Pengaruh Lama Paparan Androgen Terhadap Indeks Resistensi Insulin dan Kadar Asam Lemak Bebas Pada Serum Tikus Model Sindroma Ovarium Polikistik (Penelitian Eks-perimental dengan Rattus novergicus sebagai hewan coba). Surabaya: FK UNAIR. 2009.

Beloosesky R, Gold R, Almog B, Sasson R, Dantes A, Land-Bracha A. Induction of polycystic ovary by testosterone in immature female rats: modulat-ion of apoptosis and attenuation of glucose/ insulin ratio. International Journal of Molecular Medicine. 2004;14:207-15.

Hirshman M, Goodyear L, Wardzala L, Horton ED, Horton ES. Identification of an intracellular pool of glucose transportes from basal and insulin-stimulated rat skeletal muscle. The Journal of Biological Chemistry. 1990;285(2):987-91.

Kahn B, Rosen A, Bak J, Andersen P, Damsbo P, Lund S, et al. Expression of GLUT1 and GLUT4 glucose transportes in skeletal muscle of human with insulin-dependent Diabetes Mellitus : regulatory effects of metabolic factors. Journal of Clinical Endocrinology and Metabolism. 1992;74 (5):1101-9.

Gaster M, Staehr P, Beck-Nielsen H, Schroder HD and Handberg AA. GLUT4 is reduced in slow muscle fibers of type 2 diabetic patients. Diabetes. 2001:1324-9.

Rosenbaum D, Haber R, Dunaif A. Insulin resistance in polycystic ovary syndrome: decreased expression of GLUT4 glucose transporters in adipocytes. American Physiological Society. 1993:197-202.

Roden M, Price T, Perseghin G, Petersen K, Rothman D, Cline G. Mechanism of free fatty acid-induced insulin resistance in humans. J Clin Invest. 1996;97(12):2859-65.

Hayes GR, Lockwood DH. Role of insulin receptor phosphorylation in the insulinomimetic effects of hydrogen peroxide. Proc Natl Acad Sci. 1987;84:8115-9.

Pessler D, Rudich A, Bashan N. Oxidative stress impairs nuclear protein binding to the insulin responsive element in the GLUT4 promoter. Diabetologia. 2001;44:2156-64.

Yasuda K, Adachi T, Gu N, Matsumoto A, Matsunaga T, Tsujimoto G, Tsuda K, Ishihara A. Effects of hyperbaric exposure with high oxygen concentration on glucose and insulin levels and skeletal muscle fiber properties in diabetic rats. Muscle Nerve. 2007;35:337-43.

Houstis N, Rosen E, Lander E. Reactive oxygen species have a causal role in multiple forms of insulin resistance. Nature. 2006;440:944-7.

Barbieri E, Sestili P. Reactive oxygen species in skeletal muscle signaling. Journal of Signal Transduction. 2012.

Gill A and Bell C. Hyperbaric oxygen : its uses, mechanisms of action, and outcomes. QJ Med. 2004;97:385-95.

Wijayanto I. Pengaruh HBO pada diabetes mellitus melalui pengukuran HbA1c. Laporan Penelitian Program Pascasarjana Universitas Airlangga. 2000.

Rusdiana D. Efek oksigen hiperbarik terhadap peningkatan ekspresi Akt pada Rattus novergicus dengan diabetes mellitus. Surabaya: Laporan Penelitian Universitas Airlangga. 2014.

McGee E, Hsueh A. Initial and cyclic recruitment of ovarian follicles. Endocrine Reviews. 2000;21 (2):200-14.

Atis A, Aydin Y, Cifti F, Sakiz D, Arslan A, Toklu A. Hyperbaric oxygen increases atresia in normal & steroid induced PCO rat ovaries. Reproductive Biology and Endocrinology. 2012;10(11).


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