Curcumin reduces inflammation process in mice model preeclampsia
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HIGHLIGHTS
- Increased oxidative stress results in increased iNOS in pre-eclampsia.
- COX-2 expression in the placenta of women suffering from preeclampsia increases and is associated with increased thromboxane production.
ABSTRACT
Objective: Preeclampsia is one of the most frequently documented pregnancy complications, with a prevalence of approximately 2 to 15% of all pregnancies. Preeclampsia is a leading cause of maternal mortality. According to research in Indonesia, preeclampsia accounts for 66.8% of all cases of hypertension during pregnancy. This research aims to evaluate the impact of curcumin on serum levels of COX-2 and iNOS in a mouse model of preeclampsia.
Materials and Methods: This study employed a true experimental design with a post-test-only controlled group approach using pregnant Rattus norvegicus as a preeclampsia model. Curcumin was administered orally via a feeding tube after dissolving powdered tablets. Dosages were 30 mg/day, 50 mg/day, or 100 mg/day, adjusted for the rats' weight. Serum COX-2 and iNOS levels were measured using ELISA kits from Bioassay Technology Laboratory, with concentrations reported in pg/ml. Analysis was performed using SPSS for Windows 19.0.
Results: Serum COX-2 levels showed significant differences (p < 0.05) across groups. L-NAME treatment increased COX-2 levels compared to the negative control. Curcumin (50 and 100 mg/kgBW) reduced COX-2 levels significantly compared to the positive control, with no notable differences between curcumin doses. For iNOS levels, significant differences were also found (p < 0.05). Curcumin at 100 mg/kgBW significantly lowered iNOS levels compared to the positive control, with no significant differences between other treatment groups.
Conclusion: Curcumin administration effectively reduces COX-2 and iNOS levels in the serum of Rattus norvegicus with a preeclampsia model.
Chang KJ, Seow KM, Chen KH. Preeclampsia: Recent advances in predicting, preventing, and managing the maternal and fetal life-threatening condition. Int J Environ Res Public Health. 2023; 20(4):2994. doi: 10.3390/ijerph20042994. PMID: 36833689; PMCID: PMC9962022.
Nirupama R, Divyashree S, Janhavi P, et al. Preeclampsia: Pathophysiology and management. J Gynecol Obstet Hum Reprod. 2021;50(2):101975. doi: 10.1016/j.jogoh.2020.101975. Epub 2020 Nov 7. PMID: 33171282.
Tjandraprawira KD, Kusumah AY, Kamilah AY, et al. Management and perinatal outcomes of hypertensive disorders of pregnancy in a low-resource setting in Indonesia. SAGE Open Med. 2021;9:20503121211060194. doi: 10.1177/20503 121211060194. PMID: 34888051; PMCID: PMC8649090.
Ratsiatosika AT, Razafimanantsoa E, Andriantoky VB, et al. Incidence and natural history of preeclampsia/eclampsia at the university maternity of Antananarivo, Madagascar: high prevalence of the early-onset condition. J Matern Fetal Neonatal Med. 2019;32(19):3266-71. doi: 10.1080/1476 7058.2018.1462323. Epub 2018 Apr 22. PMID: 29621911.
Phipps EA, Thadhani R, Benzing T, et al. Pre-eclampsia: pathogenesis, novel diagnostics and therapies. Nat Rev Nephrol. 2019;15(5):275289. doi: 10.1038/s41581-019-0119-6. Erratum in: Nat Rev Nephrol. 2019 Jun;15(6):386. doi: 10.1038/ s41581-019-0156-1. PMID: 30792480; PMCID: PMC6472952.
Wang J, Guan C, Hou H, et al. A case-control study of relevance between NOS2 rs2297518 polymor-phism with preeclampsia risk in the Chinese Han women. 2021. doi: 10.21203/rs.3.rs-577358/v1.
de Alwis N, Binder NK, Mangwiro YTM, et al. Actions of esomeprazole on the maternal vasculature in lean and obese pregnant mice with impaired nitric oxide synthesis: A model of preeclampsia. Int J Mol Sci. 2022;23(15):8185. doi: 10.3390/ijms23158185. PMID: 35897759; PMCID: PMC9330120.
Kopincová J, Púzserová A, Bernátová I. L-NAME in the cardiovascular system - nitric oxide synthase activator? Pharmacol Rep. 2012;64(3):511-20. doi: 10.1016/s1734-1140(12)70846-0. PMID: 2281 4004.
Carolina Alves R, Perosa Fernandes R, Fonseca-Santos B, et al. A critical review of the properties and analytical methods for the determination of curcumin in biological and pharmaceutical matrices. Crit Rev Anal Chem. 2019;49(2):138-149. doi: 10.1080/10408347.2018.1489216. Epub 2018 Sep 25. PMID: 30252504.
Hewlings SJ, Kalman DS. Curcumin: A review of its effects on human health. Foods. 2017;6(10):92. doi: 10.3390/foods6100092. PMID: 29065496; PMCID: PMC5664031.
Kotha RR, Tareq FS, Luthria DL. Extractability of curcuminoids is enhanced with milk and aqueous-alcohol mixtures. Molecules. 2022;27(15):4883. doi: 10.3390/molecules271548 83. PMID: 3595 6834; PMCID: PMC9369953.
Zanganeh M, Mazloomi SN, Alijanpour E, et al. Curcumin and pregnancy problems: a narrative review of curcumin’s effect on preeclampsia. Journal of Clinical and Basic Research. 2020; 4(4):1-7. doi: 10.52547/jcbr.4.4.1.
Yanti E, Rahardjo B, Hidayati D, MK S. Pengaruh pemberian pravastatin terhadap kadar IFN- dan Il-10 pada serum tikus wistar (Rattus norvegicus) model preeklamsi. [Repository]. Malang, Indonesia: Universitas Brawijaya; 2021.
Vian M, Zhang Y, Liu Z, et al. The PD-1/PD-L1 inhibitory pathway is altered in pre-eclampsia and regulates T cell responses in pre-eclamptic rats. Sci Rep. 2016;6:27683. doi: 10.1038/srep27683. PMID: 27277012; PMCID: PMC4899740.
Rahardjo B, Widjajanto E, Sujuti H, et al. Curcumin decreased level of proinflammatory cytokines in monocyte cultures exposed to preeclamptic plasma by affecting the transcription factors NF-κB and PPAR-γ. Biomarkers and Genomic Medicine. 2014;6(3):105-15. doi: 10.1016/j.bgm.2014.06.002.
Zhu H, Zhu W, Hu R, et al. The effect of pre-eclampsia-like syndrome induced by L-NAME on learning and memory and hippocampal glucocorticoid receptor expression: A rat model. Hypertens Pregnancy. 2017;36(1):36-43. doi: 10.1080/10641955.2016.1228957. Epub 2016 Oct 27. PMID: 27786581.
Shu W, Li H, Gong H, et al. Evaluation of blood vessel injury, oxidative stress and circulating inflammatory factors in an L-NAME-induced preeclampsia-like rat model. Exp Ther Med. 2018; 16(2):585-94. doi: 10.3892/etm.2018.6217. Epub 2018 May 24. PMID: 30112025; PMCID: PMC609 0470.
Fadinie W, Lelo A, Wijaya DW, et al. Curcumin's Effect on COX-2 and IL-10 Serum in Pre-eclampsia's Patient Undergo Sectio Caesarea with Spinal Anesthesia. Open Access Maced J Med Sci. 2019;7(20):3376-3379. doi: 10.3889/oamjms.2019. 426. PMID: 32002054; PMCID: PMC6980822.
Zhou J, Miao H, Li X, et al. Curcumin inhibits placental inflammation to ameliorate LPS-induced adverse pregnancy outcomes in mice via upregulation of phosphorylated Akt. Inflamm Res. 2017;66(2):177-85. doi: 10.1007/s00011-016-1004-4. Epub 2016 Nov 5. PMID: 27817102.
Fu Y, Gao R, Cao Y, et al. Curcumin attenuates inflammatory responses by suppressing TLR4-mediated NF-κB signaling pathway in lipopoly-saccharide-induced mastitis in mice. Int Immuno-pharmacol. 2014;20(1):54-8. doi: 10.1016/ j.intimp. 2014.01.024. Epub 2014 Feb 6. PMID: 24508537.
Ben P, Liu J, Lu C, et al. Curcumin promotes degradation of inducible nitric oxide synthase and suppresses its enzyme activity in RAW 264.7 cells. Int Immunopharmacol. 2011;11(2):179-86. doi: 10.1016/j.intimp.2010.11.013. Epub 2010 Nov 20. PMID: 21094287.
Streyczek J, Apweiler M, Sun L, et al. Turmeric extract (Curcuma longa) mediates anti-oxidative effects by reduction of nitric oxide, iNOS Protein-, and mRNA-synthesis in BV2 microglial cells. Molecules. 2022;27(3):784. doi: 10.3390/molecules 27030784. PMID: 35164047; PMCID: PMC884 0760.
Greish SM, Abdel-Hady Z, Mohammed SS, et al. Protective potential of curcumin in L-NAME-induced hypertensive rat model: AT1R, mitochon-drial DNA synergy. Int J Physiol Pathophysiol Pharmacol. 2020;12(5):134-146. PMID: 33224436; PMCID: PMC7675192.
Mukherjee I, Singh S, Karmakar A, et al. New immune horizons in therapeutics and diagnostic approaches to preeclampsia. Am J Reprod Immunol. 2023;89(2):e13670. doi: 10.1111/ aji. 13670. Epub 2023 Jan 2. PMID: 36565013.
Mirabito Colafella KM, Neuman RI, Visser W, et al. Aspirin for the prevention and treatment of pre-eclampsia: A matter of COX-1 and/or COX-2 inhibition? Basic Clin Pharmacol Toxicol. 2020;127(2):132-141. doi: 10.1111/bcpt.13308. Epub 2019 Sep 11. PMID: 31420920; PMCID: PMC7496715.
Bansal RK, Goldsmith PC, He Y, et al. A decline in myometrial nitric oxide synthase expression is associated with labor and delivery. J Clin Invest. 1997;99(10):2502-8. doi: 10.1172/JCI119434. PMID: 9153294; PMCID: PMC508091.
Liu T, Zhang M, Mukosera GT, et al. L-NAME releases nitric oxide and potentiates subsequent nitroglycerin-mediated vasodilation. Redox Biol. 2019;26:101238. doi: 10.1016/j.redox.2019. 101238. Epub 2019 Jun 4. PMID: 31200239; PMCID: PMC6565607.
Leo MD, Kandasamy K, Subramani J, et al. Involvement of inducible nitric oxide synthase and dimethyl arginine dimethylaminohydrolase in Nω-nitro-L-arginine methyl ester (L-NAME)-induced hypertension. Cardiovasc Pathol. 2015;24(1):49-55. doi: 10.1016/j.carpath.2014.09.002. Epub 2014 Sep 18. PMID: 25294342.
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