Effect of per oral sipermetrin exposure on serum 17-beta estradiol and uterine malondialdehyde (MDA) levels in female Wistar strain rats (Rattus norvegicus)

Hesty Widowati, Hidayat Sujuti, Karyono Mintaroem

= http://dx.doi.org/10.20473/mog.V26I12018.20-25
Abstract views = 550 times | downloads = 586 times


Objective: This study aimed to verify the effect of oral siper-metrin exposure to decrease serum estradiol 17b levels and increased malondialdehyde (MDA) in the uterus level of female Wistar strain rats (Rattus norvegicus).

Materials and Methods: The method of this study was true experimental post test only control group in vivo using 24 female rats, divided into 3 groups treated by administering a dose of 5, 10 and 20 mg/kg sipermetrin for 28 days and one control group. Then blood samples were taken from the heart for measurement of serum estradiol 17b levels by ELISA and uterine organs were taken for measurement of Malondialdehyde (MDA) with spectro-photometry method.

Results: The results of the measurement of serum estradiol 17b and uterus malondialdehyde (MDA) levels of female Wistar strain rats (Rattus norvegicus) showed an opposite pattern, where there was a decline in serum estradiol 17b levels and an increase in uterus malondialdehyde (MDA) level. There was a significant difference (p=0.000<alpha) in 17b estradiol serum and uterus Malondialdehyde (MDA) levels of female rats between control group and group exposed to sipermetrin treatment for 28 days.

Conclusions: Oral sipermetrin exposure can decrease serum levels of estradiol 17b and increase uterine levels of malondi-aldehyde (MDA) of female Wistar strain rats (Rattus norvegicus).


Sipermetrin; 17-beta estradiol serum; malondialdehyde (MDA)

Full Text:



Suhartono. Dampak Pestisida Terhadap Ke-sehatan. Proceeding. Seminar Nasional Pertanian Organik. 2014.

Sangha GK, Kaur K, Khera KS 2013. Cyper-methrin induced pathological and biochemical cha-nges in reproductive organs of female rats. Journal of Environmental Biology. 2013.

Halim. Bahaya pestisida terhadap kesuburan. [cited 2017 Jan 24]. Available from: http://harian.analisa daily.com/kesehatan/news/bahaya-pestisida-terhadap-kesuburan/ 300134/2017/01/16

Saillenfait AM, Ndiaye D, Sabaté JP. Pyrethroids: Exposure and health effects. An update. Internatio-nal Journal of Hygiene and Environmental Health. 2015;218:281–92

Purba IG. Analisis Faktor-Faktor Yang Berhubu-ngan dengan Kadar Kolinesterase pada Perempuan Usia Subur di Daerah Pertanian. Thesis. Semarang: Diponegoro University; 2009.

Sun H, Chen W, Xuc X et al. Pyrethroid and their metabolite, 3-phenoxybenzoicacid showed similar (anti) estrogenic activity inhuman and rat estrogen receptor -mediated reporter gene assays. Environ-mental Toxicology and Pharmacology; 37:371–377

Porte C, Janer G, Lorusso LC, et al. Endocrine disruptors in marine organisms: Approaches and perspectives. Comparative Biochemistry and Phy-siology Part C. 2006;143:303–15.

Sankar P, Telang AG, Manimaran A. Protective effect of curcumin on cypermethrin-induced oxida-tive stress in Wistar rats. Experimental and Toxi-cologic Pathology. 2012;64:487–93.

Guo D, Wang Y, Qian Y, et al. Joint acute and endocrine disruptive toxicities of malathion, cyper-methrin and prochloraz to embryo-larval zebrafish, Chemosphere. 2016; 166:63-71

Kiyama R and Wada-Kiyama Y. Estrogenic endo-crine disruptors: Molecular mechanisms of action. Environment International. 2015;83:11–40

Bretveld RW, Thomas CMG, Scheepers PTJ et al. Pesticide exposure: the hormonal function of the female reproductive system disrupted? Reproduc-tive Biology and Endocrinology. 2006; 4:30

Nazrun AS, Khairunnur A, Norliza M, et al. Effects of palm tocotrienols on oxidative stress and bone strength in ovariectomised rats. Med & Health. 2008;3(2):247-55.

Khazri A, Sellami B, Dellali M et al. 2015. Acute toxicity of cypermethrin on the fresh water mussel Unio gibbu. Ecotoxicology and Environmental Safety. 2015;115:62–6.

Giray B, Gurbay A, Hincal F. Cypermethrin-induced oxidative stress in rat brain and liver is prevented by Vitamin E or allopurinol. Toxicology Letters. 2001;118:139–46.

Khatab AE., Hashem NM, El-Kodary LM et al. Evaluation of the effects of cypermethrin on female reproductive function by using rabbit model and of the protective role of chinese propolis. Biomedical Environmental Science. 2016;29(10):762-66.

Singh Z, Karthigesu IP, Singh PKR. Use of malon-dialdehyde as a biomarker for assessing oxidative stress in different disease pathologies: A review. Iranian J Publ Health. 2014;43(Suppl. 3): 7-16.

McCarthy ARM, Thomson B, Shaw IC, Abella AD. Estrogenicity of pyrethroid insecticide metabolites. Journal of Environmental Monitoring. 2005.

Saravanan N, Uma T, Narendiran NJ. Effect of cypermethrin on estradiol and vitellogenin in the tissues of Esomus danricus. Journal of Entomology and Zoology Studies. 2016;4(5):524-28.

Singh PB and Singh V. Cypermethrin induced histological changes in gonadotrophic cells, liver, gonads, plasma levels of estradiol-17b and 11-keto-testosterone, and sperm motility in Heteropneustes fossilis (Bloch). Chemosphere. 2008. 72:422–31.

Kelly MJ, Qiu J, Ronnekleiv OK. Estrogen signal-ing in the hypothalamus, vitamins and hormones. Vitam Horm. 2005;71:123-45.

Singh AK, Nath T, Manindra OP, Singh MP. 2012. A current review of cypermethrin-induced neuro-toxicity and nigrostriatal dopaminergic neurodege-neration. Current Neuropharmacology. 2012;10:64-71.

Muthuviveganandavel V, Muthuraman P, Muthu S, Srikumar K. 2008. A study on low dose cyperme-thrin induced histopathology, lipid peroxidation and marker enzyme changes in male rat. Pesticide Biochemistry and Physiology. 2008; 91:12–6.

Banke IS, Folorunsho AS, Mohammed B, et al. Effects of melatonin on changes in cognitive per-formances and brain malondialdehyde concen-tration induced by sub-chronic coadministration of chlorpyrifos and cypermethrin in male Wister rats. Asian Pac J Trop Biomed 2014;4(4):318-23.

Del Rio D, Stewart AJ, Pellegrini N. A review of recent studies on malondialdehyde as toxic mole-cule and biological marker of oxidative stress. Nutr Metab Cardiovasc Dis. 2005;15(4):316-28.

Idris SB, Ambali SF, Ayo JO. Cytotoxicity of chlopyrifos and cypermethrin: The ameliorative effects of antioxidants. African Journal of Biotechnology. 2012;11(99):16461-7.

Yuan L, Dietrich AK, Nardulli AM. 17b-Estradiol alters oxidative stress response protein expression and oxidative damage in the uterus. Molecular and Cellular Endocrinology. 2014;382(1):218-26.

Stirone C, Duckles SP, Krause DN, Procaccio V. Estrogen increases mitochondrial efficiency and reduces oxidative stress in cerebral blood vessels. Molecular Pharmacology. 2005;68(4).

Tamura H, Takasaki A, Taketani T, et al. 2012. The role of melatonin as an antioxidant in the follicle. J Ovarian Res. 2012;26(5):5.

Gomez-Zubeldia M, Hinchado AG, Arbue´s JJ, et al. Influence of estradiol on oxidative stress in the castrated rat uterus. Gynecologic Oncology. 2001; 80:227–32.


  • There are currently no refbacks.

Copyright (c) 2018 Majalah Obstetri & Ginekologi



Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License