Pengaruh Monoklonal Antibodi Bovine Zona Pelusida 3 (bZP3) terhadap Diameter dan Atresia Folikel Ovarium Mencit (Mus musculus)

Annisa Trissatharra, Sri Ratna Dwiningsih, Ratna Sofaria Munir

Abstract views = 259 times | views = 525 times


Objectives: To identify the effect of monoclonal antibody bZP3 at ovarian follicles that undergo atresia and diameter of various ovarian follicles.

Materials and Methods: This is a true experimental research with post only control group design. Samples were 36 female mices (Mus musculus) which is divided into 6 groups, there are 3 control groups (group 1, 2, and 3) injected by Phospatase Buffer Saline (PBS) 50µl and 3 treatment groups (group 4, 5, and 6) injected by Mab bZP3 50µl. Group 1 and 4 terminated at 5th day, group 2 and 5 terminated at 10th day, and group 3 and 6 terminated at 20th day. Evaluation of atretic ovarian follicles and diameter of ovarian follicles performed by hematoxylin eosin (HE) and the data processed by parametric statistic.

Results: There are no significant in different among groups in the aspect of atretic follicles and diameter of folicles (p>0.05), but descriptively, number of follicles undergo atresia of the follicle primary, secondary, and tertiary treatment group was higher than the control group, except on the 20th day of observation time.

Conclusion: administration of Mab bZP3 had no effect to amount of atretic follicles and diameter of folicles during observation time.


Mab bZP3, atretic follicles, diameter follicles

Full Text:



Gupta SK, Bansal P, Ganguly A, Bhandari B dan Chakrabarti K. Human Zona Pellusida Glycoprotein: Functional Relevance During Fertilization. Journal Of Reproductive Immuno-logy. 2009;83:50-5.

Sumitro SB, Aulanni’am, Soewarto S, Ciptadi G, Widyarti S dan Kurniawan D. Imunokontrasepsi Konsep-Konsep Dasar Dan Bunga Rampai Penelitian. Surabaya: Pusat Penerbitan Dan Percetakan Unair (AUP); 2011. h. 1-95.

Chang RjdE G. Folliculogenesi, Ovulation, and Luteogenesis in Endocrinology. 6th Eds. Saunder Elsevier; 2010.

Erickson GF. Follicle Growth and Development, Global Library Women’s Med. Follicle Growth and Development. Global Library Women’s Med. 2008.

Gougeon A. Human Ovarian Follicular Development: From Activation of Resting Follicle to Preovulatory Maturation. Anales d’Endocrino-logies. 2010;21:132-43.

Colongos G, Hasegawa A, Komori S and Koyama K. Harmful Effect Of Anti-Zona Pellusida Antibodies in Folliculogenesis, Oogen-esis, and Fertilization. Journal of Reproductive Immunology. 2009;79:148-55.

Putra A Sumarno dan Sundari S. Pertemuan Ilmiah Bidang Imuno-histokimia dan Western Blot. Semarang. Fakultas kedokteran Universitas Sultan Agung. 2012.

Paixao L, Gaspa-Reis RP, Gonzalea G, Santos A, Santana AC, Santos RM, Spritzer PM, Nascimento-Saba CCA. Cigarette Smoke Impair Granulosa Cells Proliferation and Oocyte Growth After Exposure Cessation in Young Swiss Mice: An Experimental Stud. J. Ovarian Rep. 2012;5(1):24.

Caligioni C. Assessing reproductive status/stages in mice. Curr Protoc Neurosci. 2009;41(10):1-11.

Naz RK and Rajesh C. Passive Immunization For Immunocontraception: Lesson Learned From Infectious Diseases, Frontiers in Bioscience. 2004;9(1):2457-65.

Keizer RJ, Huitema AD, Schellens JH and Beijnen JH. Clinical Pharmacokinetics of Therapeutic Monoclonal Antibodies. J. Clinical Pharmacokinetics. 2010;49(8):493-507.

Baratawidjaja KG dan Rengganis I. Imunologi Dasar. Edisi ke-10. Jakarta: FK Universitas Indonesia; 2013. p. 2-618.

Matsuda F, Naoko I, Manabe N Dan Ohkura, S. Follicular Growth and Atresia In Mammalian Ovaries: Regulation by Survival and Death of Granulosa Cells. Reproduction and Development. 2012;58(1):44-50.

Abbas AK and Lichtman AH. Basic Imunology. 3rd Eds. Philadelphia: WB Saunders Company; 2011.


  • There are currently no refbacks.

Copyright (c) 2018 Majalah Obstetri & Ginekologi



Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License