Ovozoa: Journal of Animal Reproduction

Spermatozoa motility and viability of turkey (Meleagris gallopavo) semen in egg yolk nanoparticles-phosphate buffered saline extender at 3-5°C storage

2024-10-21T13:46:23+07:00

This study aimed to evaluate the spermatozoa motility and viability of turkey semen extended with egg yolk nanoparticles-PBS extender at various concentrations, stored at 3-5°C. Semen was collected from two turkeys and divided into four treatment groups. Group T0 consisted of fresh turkey semen extended with 20% (v/v) fresh egg yolk in phosphate-buffered saline (PBS). Groups T1, T2, and T3 consisted of fresh turkey semen extended with 5, 10, and 20% (v/v) egg yolk nanoparticles in PBS, respectively. Extended semen was stored in a refrigerator (3-5°C), and progressive spermatozoa motility and viability were evaluated every two hours until spermatozoa motility decreased to 40%. The results showed that turkey semen in T3 extender containing 20% egg yolk nanoparticles in PBS was able to maintain spermatozoa motility for up to eight hours, better than T0 and T2 extenders which supported spermatozoa motility for six hours. In contrast, T1 extenders only support spermatozoa motility for four hours. T3 extenders were also able to maintain spermatozoa viability for up to eight hours, while T0, T1 and T2 extenders could only support viability for 6 hours. The extender with 20% egg yolk nanoparticles in PBS was significantly better (p <0.05) in maintaining spermatozoa quality compared to those with 5 and 10% egg yolk nanoparticles, as well as the extender with 20% fresh egg yolk. In conclusion, 20% egg yolk nanoparticles in PBS semen extender was effective in maintaining the motility and viability of turkey (Meleagris gallopavo) spermatozoa stored at 3-5°C for up to eight hours.

Estrus response in dairy cows surviving foot and mouth disease given Superbooster and Immunobooster

2024-11-13T11:16:44+07:00

This study aims to determine the effect of adding Superbooster and Immunobooster on the estrus response of dairy cows after foot and mouth disease (FMD) infection. This study used 18 dairy cows that recovered from FMD based on veterinarian examination. Cows were divided into three groups (T0, T1 and T2). In the control group (T0), cows were fed standard feed. Standard feed consisted of forage (10% of body weight, daily) and concentrate. In the T1 group, cows were fed standard feed added with Superbooster (40 g daily), and in the T2 group cows were fed standard feed added with Superbooster (40 g daily) and Immunobooster (800 g daily). This treatment was carried out for 42 days. The results showed that the estrus rate in T1 and T2 were both 100%, which was higher compared to the control (16.67%). The onset of estrus was shorter (p <0.05) compared to the duration of the last estrus until the start of treatment. Meanwhile, the duration of the last estrus until the day of treatment and onset of estrus between treatment groups were not significantly different (p <0.05). It could be concluded that Superbooster and Immunobooster could stimulate estrus on dairy cows after FMD.

Uterine prolapse and related factors in beef cow at the Besuki Animal Health Center, Situbondo regency, East Java, Indonesia

2024-11-20T22:20:21+07:00

This study aims to determine the factors related to uterine prolapse in beef cow in the Besuki Animal Health Center working area in Situbondo regency. The sample consisted of 100 cows that calved in 2020-2022, whose data was obtained from the Department of Animal Husbandry of Situbondo regency, East Java, Indonesia. Determination of the months of the rainy and dry season was obtained from the Meteorological, Climatological and Geophysical Agency, Juanda Class I Meteorological Station. Other data regarding breed, age, parity of cow and dystocia incidence in cow was obtained by conducting interviews with farmers followed by confirmation with inseminator records, and direct observation of the housing system. The collected data were cross-tabulated and analyzed using Chi-square analysis to determine possible factors causing uterine prolapse. The results showed that the incidence of uterine prolapse in beef cow in the working area was 35% (35/100). The factors of housing, age, parity, and incidence of dystocia were significantly related (p <0.05) to the incidence of uterine prolapse, while breed and season did not have a significant influence (p > 0.05). It could be concluded that the age and parity of the cow at the time of calving, the practice of housing cow, and the incidence of dystocia were related to the incidence of uterine prolapse in cows in the Besuki Animal Health Center operational area.

Ethanolic extract of Dayak onion (Eleutherine palmifolia) prevented sperm membran damage in mice exposed to monosodium glutamate

2024-11-13T11:18:41+07:00

Monosodium glutamate (MSG) could cause increased production of reactive oxygen species (ROS), causing oxidative stress in the testicles, which adversely affected sperm quality. Dayak onion extract which is known for its high antioxidant content, could help alleviate oxidative stress caused by MSG. This research examined the effect of Dayak onion (Eleutherine palmifolia) on sperm abnormalities and plasma membrane integrity in mice (Mus musculus) exposed to MSG. Twenty-five male mice, 8 weeks old with a body weight approximately 20g, were divided into five groups, C– (received 0.5% Na-CMC), C+ (received 4 mg/g bw MSG), and three treatment groups (T1, T2, T3) which were given 4 mg/g bw MSG accompanied by Dayak onion extract at doses of 30, 60, and 120 mg/kg bw respectively. All treatments lasted for 52 days. Significant differences (p <0.05) in sperm abnormalities and plasma membrane integrity were observed among the groups. Sperm abnormalities found were (12.3 ± 1.92), (61.1 ± 3.10), (41.6 ± 2.87), (30.4± 1.91), and (18.2 ± 2.10) % respectively for C–, C+, T1, T2 and T3. Meanwhile, sperm plasma membrane integrity found were (33.0 ± 3.24), (69.3 ± 2.32), (41.8 ± 2.42), (55.4 ± 3.11), and (64.2 ± 1.27) % respectively for C–, C+, T1, T2 and T3. These results indicate that Dayak onion extract could help reduce sperm abnormalities and maintain plasma membrane integrity in mice exposed to MSG.

Addition of basil leaf (Ocimum basicilum L.) extract to AndroMed® extender improved the quality of spermatozoa of frozen thawed Simmental bull semen

2024-11-13T11:21:01+07:00

This study aims to determine the best dose of basil leaf extract to add to AndroMed® semen extender to improve spermatozoa quality after freezing and thawing. This study used healthy, normal four-year-old Simmental bulls with good libido. The sample used was fresh semen from Simmental bull collected using an artificial vagina. This study used a completely randomized design (CRD) with three treatments and six replicates. Ejaculate that met the motility qualification of more than 70%, was divided into three groups, each extended in AndroMed® without the addition of basil leaf extract (T0), with the addition of 0.1% basil leaf extract (T1) and with the addition of 0.3% basil leaf extract (T2). Straw containing semen with 30 million spermatozoa/straw was processed using an IceCube automatic freezer (Minitube, Germany). The results showed that spermatozoa motility, viability, and spermatozoa plasma membrane integrity in the T2 group were higher (p <0.05) than those in the T0 and T1 groups, while spermatozoa morphological abnormalities in the T2 group were lower (p <0.05) than those in the T0 and T1 groups. There were no significant differences (p >0.05) in these parameters between groups T0 and T1. It could be concluded that the addition of 0.3% basil leaf extract to the AndroMed® extender could improve the quality of the semen of Simmental bulls after thawing.

Effect of age of Awassi ewes crossed with Naimi rams on some growth characteristics and litter size

2024-10-23T08:43:41+07:00

The study focused on Awassi ewes crossed with Naimi rams. Ewes weight and age were closely related to their productivity, especially on female reproductive performance, therefore this research studied the effect of ewes age to their weight, as well as the effect of the ewes age on the weight of their lambs at birth, at one month, 4 month and to their sex and fertility. In the Awassi ewes used in this study, mature body size was attained at the age of 4 then it started to decline. At lambing, the lambs whose mothers were at the age of 5 years outperformed the rest of the age groups of their mothers, and at one month, the lambs whose mothers were at the age of 3 years outperformed (p ≤0.05) the rest of the age groups of their mothers. The weight of the lambs at weaning (4 months of age) showed the highest in the lambs whose mothers were 3 to 4 years old. The largest number of male lambs were obtained from ewes aged 2.5 and 3 years (75 and 88.9% respectively). Singleton births were 100% for all ewe ages, except for the ewes aged 2.5 and 3 years (40 and 20%, respectively). The highest number of twin births (80%) (p ≤0.05) was obtained in lambs whose mothers were 3 years old, while the highest litter size was 1.8.

Incidence and management of dystocia of dairy cows in Manoharganj upazila of Cumilla district, Bangladesh

2024-11-14T11:29:19+07:00

Every year, thousands of cows and calves die during parturition for poor parturition managements, and insufficient veterinary services. A total of 83 cases of dystocia were the subject of a retrospective investigation between January 2021 and December 2023 to determine the incidence of dystocia in dairy cows in Manoharganj upazila of Cumilla district, Bangladesh. The study revealed that local breed cows had a higher prevalence of dystocia (53.01%) than cross-breed cows (46.99%). Also, younger cows had a higher incidence of dystocia (71.08%) than older cows. Primiparous heifers showed a higher incidence of dystocia (69.88%) than pluriparous cows (30.12%). In addition, compared with naturally inseminated cows (9.64%), artificially inseminated dairy cows had a higher dystocia rate (90.36%). The occurrence of dystocia was found to occur more frequently in fetal causes (65.06%) than in maternal causes (34.94%). Among maternal causes, incomplete cervix dilation was the most common (18.07%), while fetal head deviation was the most frequent fetal cause (27.71%). Fetal oversize, carpal flexion, shoulder flexion, fetal dropsy, twin calves, uterine torsion, and uterine inertia were other low-incidence causes of dystocia. The most common method of managing dystocia was manual correction (91.57%), followed by fetotomy (4.82%), and C-section (3.61%). After dystocia treatment, 96.25% of dams survived, and 3.75% of cases resulted in dam deaths; fetal death occurred in 32.5% of cases, while the fetal survival rate was 67.5%. Therefore, this study suggest that when using artificial insemination, it is best to consider the breed of the dam and sire.