Ovozoa : Journal of Animal Reproduction 2022-08-17T01:28:26+00:00 Prof. Dr. Imam Mustofa Open Journal Systems <p>OVOZOA: Journal of Animal Reproduction (OVOZOA J AnimReprod) <a href="" target="_blank">p-ISSN: 2302-6464</a>; <a href="" target="_self">e-ISSN: 2722-967X</a> is published periodically three times a year (April, August, and December) by the Department of Veterinary Reproduction Faculty of Veterinary Medicine Universitas Airlangga in collaboration with <a href="" target="_blank">the Indonesian Association of Department of Veterinary Reproduction</a>. Since March 27, 2021 OVOZOA Journal of Animal Reproduction has also collaborated with <a href="" target="_blank">the Indonesian Animal Reproduction Association</a></p><p><strong>Focus and Scope</strong></p><p>This journal published original articles, review articles, and case studies in Indonesian or English, in the scope of veterinary, animal, and biotechnology of reproduction from researchers, lecturers, students, and other practitioners around Indonesia and worldwide.</p><p><strong>Announcement</strong><br />Starting in 2020 OVOZOA Journal of Animal Reproduction is published three times per year (April, August, and December)</p><p> </p> Effect of fruit juices in skim milk extender in maintaining Sapudi ram spermatozoa quality at chilled temperature 2022-05-18T01:49:49+00:00 Ayun Tria Marga Retta Suherni Susilowati Sri Pantja Madyawati Tatik Hernawati Wurlina Wurlina Retno Sri Wahjuni <p>This study aimed to determine the effect of the addition of cherry tomato, california papaya, and honey melon juice into skim milk extender in maintaining the quality of Sapudi ram semen at chilled temperature (5<sup>o</sup>C) storage. Five replication of ejaculates were divided equally into four groups. In control group (T0) semen was diluted in skim milk extender, while in T1, T2, and T3 groups semen were diluted in skim milk extender contained 20% of cherry tomato, california papaya, and honey melon juice, respectively. The extended semen was stored at a chilled temperature, and semen quality (based on sperm motility, viability, and plasma membrane integrity) was observed daily for five days. The result showed that semen quality declined day by day during the five days of storage. Based on the minimum standard of post-thawed semen motility (40%), the spermatozoa of the control group only lasted by the second day. Meanwhile, in the groups with the addition of fruit juice motility could last up to the third day, with the highest motility (p &lt;0.05) on the addition of california papaya juice. This study concluded that the addition of 20% (v/v) california papaya juice in skim milk extender could maintain the percentage of spermatozoa motility up to three days at a chilled temperature.</p> 2022-08-17T00:00:00+00:00 Copyright (c) 2022 Ayun Tria Marga Retta, Suherni Susilowati, Sri Pantja Madyawati, Tatik Hernawati, Wurlina Wurlina, Retno Sri Wahjuni Cranium morphometry for distinguishing male and female Muscovy duck (Cairina moschata) before sexual maturity 2022-06-13T15:39:49+00:00 Mafatichul Firdaus Abdul Samik Yeni Dhamayanti Raden Tatang Santanu Adikara Hana Eliyani Soeharsono Soeharsono <p>Gender determination of Muscovy duck as early as possible is essential for the efficiency and effectiveness of duck production. This study aimed to determine the morphometric differences in prepubertal male and female Muscovy duck (<em>Cairina moschata</em>) as predictors of gender. This study used a split-plot design, with the observed variables cranium length, width and height, mandibular length, and rostrum length and width. This study used a total of 80 samples of duck heads consisting of 40 male and 40 female Muscovy duck heads, with ten heads each in the age group of 2, 4, 6, and 8 weeks, respectively. Results showed that the length, width, and height of the cranium, mandibular length, and rostrum lengthand width were greater in males (p &lt;0.05) than those of the females in all age groups. It could be concluded that the morphometric size of prepubertal male Muscovy ducks head was greater than those of the female ones at the same age; thereby, it could be used to distinguish gender. Based on the morphometrics, further study can also be conducted to measure other body parts of the Muscovy duck apart from the cranium. In addition, it is necessary to study the morphometric measurements of the cranium in other poultry as a basis for identifying sex.</p> 2022-08-17T00:00:00+00:00 Copyright (c) 2022 Firdaus Determination of estrus duration based on cervical mucus characteristics in Aceh cattle using camera-equipped artificial insemination endoscope 2022-07-13T01:44:15+00:00 Ginta Riady drh., M.Sc Dian Mulfristia Hendra Saputra Cut Nila Thasmi Hafizuddin Hafizuddin Mudhita Zikkrullah Ritonga Rosmaidar Rosmaidar <p>This study aimed to determine the length of the estrus period in Aceh cows based on changes in the characteristics of cervical mucus observed using a camera-equipped artificial insemination (AI) endoscope. This study used twelve healthy and reproductively sound cows aged 4-6 years. All cows were synchronized using prostaglandin F2α (PGF2α) injected intramuscularly twice, 11 days apart. Estrus detection was conducted 24 hours following the second injection of PGF2α three times daily for 30 minutes, respectively. Estrous cows were then subjected to the examination of their cervical mucus at 0, 6, 12, and 18 hours after the beginning of estrus. This study showed that out of 12 samples, only eight cows exhibited estrus signs. Characteristics of cervical mucus of cows at 0 and 6 hours of estrus were transparent. At 12 hours of estrus, five cows showed transparency, and the remaining three cows showed cloudy cervical mucus. Meanwhile, at 18 hours of estrus, all sampled cows showed cloudy cervical mucus then regarded as the end of the estrus. That means the estrus length of Aceh cows in this study was 15.75 ± 3.11 hours. It can be concluded that the determination of the duration of estrus in Aceh cows based on changes in the characteristics of the cervical mucus can be carried out using an AI endoscope with a camera. For further research, it is suggested to apply this estrus detection technique in the implementation of AI and determine the pregnancy rate.</p> 2022-08-17T00:00:00+00:00 Copyright (c) 2022 Ginta Riady drh., M.Sc Effect of taurine on histopathological features of spermatogenesis in seminiferous tubules of mice (Mus musculus) induced by paraquat 2022-07-08T13:08:05+00:00 Yustisiane Ruth Rahadi Tri Wahyu Suprayogi Rahmi Sugihartuti Kadek Rachmawati Hani Plumeriastuti <p>This study aimed to determine the effect of taurine on the enhancement of the spermatogenetic process in male mice (<em>Mus musculus</em>) induced by paraquat (PQ). Twenty-five male mice (<em>Mus musculus</em>) aged 2-3 months with a bodyweight of around 35 grams were divided randomly into five groups. The K + and the treatment group (P1, P2, and P3) mice were induced using PQ. PQ was given intraperitoneally (IP) twice a week for 21 consecutive days at a dose of 30 mg/kg BW. Two hours after the administration of PQ, P1, P2, and P3 groups were given taurine at a dose of 250, 500, and 1000 mg/kg BW/day for three weeks (<a href="#Heidari">Heidari <em>et al</em>., 2019</a>). K- group was given distilled water (IP) only. On day-29, mice were sacrificed for testicles histopathological preparations with hematoxylin-eosin staining. Results showed that the mice exposed to PQ only (the K+ group) had a reduced spermatogenesis score compared to those of the K- group (p &lt;0.05). Taurine treatment on PQ-exposed mice was followed by an increase spermatogenesis score. The optimal curative dose of taurine was 500 mg/kg (P2 group). However, a higher dose (1000 mg/kg BW) of taurine resulted in a decline in the spermatogenesis score than those of at the 500 mg/kg. It could be concluded that treatment with taurine could enhance the spermatogenetic process of male mice (<em>Mus musculus</em>) induced by PQ.</p> 2022-08-17T00:00:00+00:00 Copyright (c) 2022 Yustisiane Ruth Rahadi, Tri Wahyu Suprayogi, Rahmi Sugihartuti, Kadek Rachmawati, Hani Plumeriastuti Antibiotic sensitivity test of Escherichia coli and Staphylococcus aureus isolated from the reproductive tract of dairy cows 2022-06-25T05:07:12+00:00 Baswendra Triadi Suwarno Suwarno Suryanie Sarudji Ratna Damayanti Rahmi Sugihartuti Agnes Theresia Soelih Estoepangesti <p>The purpose of this study was to determine the sensitivity of <em>Staphylococcus aureus</em> and <em>Escherichia coli</em> isolated from the reproductive tract of dairy cows during estrus to tetracycline, gentamicin, amoxicillin, and ciprofloxacin. Isolation and identification of <em>E. coli</em> and <em>S. aureus</em> from 24 cervical mucus samples resulted 15 samples containing <em>E. coli</em> and one sample containing <em>S. aureus</em>. Sensitivity test was performed using Kirby-Bauer (disc diffusion) method and interpreted using the Clinical and Laboratory Standards Institute (CLSI, 2017) guidelines. The sensitivity test of 15 isolates of <em>E. coli</em> showed 100% sensitivity to ciprofloxacin and tetracycline. For gentamicin, 14 (93.3%) isolates were sensitive, and 1 (6.7%) isolate was resistant, while for amoxicillin, 14 (93.3%) isolates were sensitive, and 1 (6.7%) isolate were intermediate. The sensitivity test of one isolate of <em>S</em>. <em>aureus</em> showed 100% sensitivity to ciprofloxacin, gentamicin, and amoxicillin; as for tetracycline 100% isolates were declared resistant.</p> 2022-08-17T00:00:00+00:00 Copyright (c) 2022 Baswendra Triadi, Suwarno Suwarno, Suryanie Sarudji, Ratna Damayanti, Rahmi Sugihartuti, Agnes Theresia Soelih Estoepangesti Partial albinism in Hawksbill turtle (Eretmochelys imbricata) in Rio Grande do Norte, Brazil 2022-07-24T12:25:53+00:00 Emanuel Lucas Rocha Lucas Gabriel Verissimo Pinheiro da Silva Isadora Nathalia Rocha Barreto Hélio Noberto de Araújo Júnior Julio Alejandro Navoni Moacir Franco de Oliveira Carlos Eduardo Bezerra de Moura <p>Albinism is a genetic disorder in which there is a defect in melanin production, a pigment which gives color to the skin, hair and eyes. This congenital malformation can also occur in sea turtles, although it is not fatal, but it reduces their chances of survival in the natural environment. Even so, there are still no reports of its occurrence in <em>Eretmochelys imbricata</em>, a species known as the hawksbill turtle or legitimate turtle, with wide distribution in tropical seas. The present study describes the case of an individual with partial albinism in the <em>E. imbricata</em> species, found on the northern coast of Rio Grande do Norte, Brazil. The animals were collected at Cabo de São Roque beach in January 2020 during the reproductive season of the species. After analyzing the animal, it can be classified as having partial piebald albinism, since it still has lighter spots both on the skin and in the carapace and plastron regions. </p> 2022-08-17T00:00:00+00:00 Copyright (c) 2022 Emanuel Lucas Bezerra Rocha, Lucas Gabriel Verissimo Pinheiro da Silva, Isadora Nathalia Rocha Barreto, Julio Alejandro Navoni, Hélio Noberto de Araújo Júnior, Moacir Franco de Oliveira, Carlos Eduardo Bezerra de Moura Bulldog calf born to Limousin-Peranakan-Ongole crossbred cow after insemination with Simmental bull semen 2022-07-17T15:05:38+00:00 Yuriati <p style="margin: 0cm; text-align: justify; text-indent: 21.3pt;">This case describes a bulldog calf born to Limousin-Peranakan-Ongole (LimPO) crossbred cow after insemination with frozen-thawed Simmental bull semen. The dam was a four year old LimPO crossbred cow, with a bodyweight of 380 kg; she was 8.5 months pregnant and has never had any reproductive disorders before. Signs of impending calving appeared at 6 am, and the discharge was normal, but until noon the calf had not been born. Body temperature was 38°C, while other vital signs were normal. Vaginal exploration revealed a completely opened cervix, and the calf was alive, in posterior longitudinal presentation with palpable short hindlegs. The calf was delivered per vaginam with traction. The calf was male with a weight of 15 kg, and was alive, but after a few minutes, the calf died. The monstrous calf was found with morphological defects including a smaller compressed body, head resembled those of a bulldog with a short muzzle and an undershot jaw, shortened fore- and hindlegs, and ascites abdomen. The dam was given 70, 000 IU procaine penicillin G and 70 mg dihydrostreptomycin sulphate (Penstrep-400, Interchemie, Holland), 0,3 g diphenhydramine HCl (Vetadryl, Sanbe, Indonesia), 10 mg vitamin B12 (Jectavit B12, Sanbe, Indonesia) by intramuscular injection. The dam looked healthy, and had normal appetite after the delivery assistance.</p> 2022-08-17T00:00:00+00:00 Copyright (c) 2022 Yuriati