Ovozoa: Journal of Animal Reproduction

Dystocia management in a Scottish Fold cat with caesarean section and ovariohysterectomy at Winadivet clinic Malang

Intan Firdha Olien Noor Al Ichsan — 2025-04-01

The causes of dystocia can be categorized into maternal and fetal factors. Dystocia is most commonly observed in queens that are giving birth for the first time. This study aims to assess the diagnosis and management of maternal dystocia in Scottish Fold cats through caesarean section (C-section). A general examination, including palpation, revealed the presence of a fetus, followed by an ultrasound to determine the number of fetuses and their viability. A hematological examination was conducted prior to the surgical procedure to assess the presence of anemia in the patient. A C-section was performed on the ventral aspect of the abdomen, followed by ovariohysterectomy after the successful removal of two live fetuses. Postoperative care involved the administration of the antibiotic Colibact, the anti-inflammatory meloxicam, supportive therapy including Channa Lakta (lactation stimulant), Channafit (immunomodulator), and the multivitamin Pantex. The results indicated that the wound of the patient, Koko cat, had closed by the third postoperative day. It can be concluded that appropriate treatment and management contribute to a rapid recovery process.

Estrus quality, non-return rate and conception rate in the incidence of repeat breeding in dairy cow after 2%povidone iodine therapy combined with prostaglandin F2α at KUD Tani Wilis Tulungagung

Amira Halimah Zahrorona — 2025-04-01

Repeat breeding is a condition in which cows fail to conceive after being bred three or more times. This study aims to determine the quality of estrus, non-return rate (NRR) and conception rates obtained after cows were treated with 2% povidone iodine. The study involved 30 Holstein Friesian cows, each having calved three times and experiencing repeat breeding. Cows in estrus were treated with 10 mL of 2% povidone iodine intrauterinely, followed by an intramuscular injection of 2 mL (250 mg/head) of prostaglandin F_2α (PGF_2α, Luteosyl) five days later. Estrus quality was observed by examining changes in vulvar mucosa color, mucus discharge, and vulvar temperature. Pregnancy diagnosis was made at 60 days. Results showed that 43.3% of cows had estrus quality score 3 (cows showed dark red vulvar mucosa, transparent mucus, temperature 38.9 ± 0.5 ºC), 23.3% had score 2 (cows showed reddish vulvar mucosa, some cloudy mucus, temperature 38.7 ± 1.0 ºC), and 33.3% had score 1 (cows showed pink vulvar mucosa, cloudy mucus, temperature 38.6 ± 0.5 ºC). Of the 30 cows, 20 had a non-return rate (NRR) of 66.7%. Based on these results, it can be concluded that 2% povidone iodine has a positive effect on estrus quality, NRR, and conception rates in dairy cows experiencing repeat breeding.

Performance of pre-breeding cycle-staging techniques in bitches: Clinical comparability of vaginal cytology and progesterone assay

Samuel Ayodele Famakinde — 2025-04-01

Accurate timing of insemination (mating) remains a critical challenge in the reproductive management of bitches. In spite of technical advances in vaginal cytology (VC) and serum-progesterone (SP) assay, the challenges appeared not to have abated. Five clinical scenarios involving VC and SP are presented, aimed at investigating technique-performance and creating more robust awareness on application of SP titers obtained using a fluorescence immunoassay kit having 7.6–20.0 ng/mL as predictable-progesterone-titer-range-for-insemination-(PPTRi). Number of bitches, insemination-readiness-detection-technique(s) (IRDT), score (%) of agreement/disagreement between Insemination-readiness-detection techniques (SABT) for peculiarities of bred bitch(es) (PBB), proportion of bitches that became pregnant confirmed by relaxin assay and, number of pups littered were studied. Results obtained for number of bitches, IRDT, SABT or PBB, relaxin and number of pups littered, respectively were: 22, VC-SP, 81.8%/18.2%, 72.7% and, NA (i.e. not-available) (case I); 2, VC-SP, bitches mated after attaining PPTRi 10 and 13 days after been detected ready-for-mating via VC, 100%, and, 5 and 7, respectively (case II); 1, SP, bitch mated at SP of 4.401 ng/mL, 100% and, 11 (case III); 2, SP, first bitch mated at 4.682 ng/mL and the second at 7.049 ng/mL, 100% and, 9 and 12, respectively (case IV); 1, VC-SP, bitch mated once detected ready-for-mating by VC while SP at 24-hours post-mating was 2.852 ng/ mL, 100% and, 1 (case V). These findings suggest high-level agreement in accuracy of Insemination-readiness-detection between the two techniques, with none reflecting superiority. For better breeding successes, it is recommendable to inseminate earlier, during the peri-ovulation period.

A study on the beneficial effect of advocating prophylactic spaying of young nulliparous bitches to prevent pyometra

Sharveihn Sathindren — 2025-04-01

This study aimed to evaluate the potential benefits of advocating elective spaying (ES) in young nulliparous bitches as a preventive measure for pyometra, a severe and potentially life-threatening condition. Specifically, the study examined whether elective spaying, which eliminates the risk of pyometra and its associated complications, offers significant advantages over the treatment of pyometra once it develops. Generally, the short- and long-term risks associated with elective spaying are minimal when compared to the significant complications and dangers posed by pyometra. A retrospective open-ended study was conducted, collecting data from veterinary clinics across Penang Island using a structured questionnaire. Information was gathered primarily from medical records and interviews with veterinarians and pet owners when data were missing or unclear in the records. The study compared 30 bitches of various breeds who underwent elective spaying with 30 bitches who underwent emergency ovariohysterectomy (EOH) due to pyometra. A standard set of outcomes was evaluated to assess and compare complications. The results showed that the elective spaying group experienced only minor or trivial complications, and owners incurred significantly lower costs compared to those whose bitches underwent emergency surgery for pyometra, which was associated with more severe complications and substantially higher costs. Based on these findings, the study concluded that elective spaying is a safe and cost-effective prophylactic measure against pyometra.

Synergistic protective effects of α-tocopherol and zinc sulfate on superoxide dismutase activity and p53 expression in ovarian granulosa cells of lead-exposed female Wistar rats (Rattus norvegicus)

iqbal dwi warsito — 2025-04-01

This study aimed to evaluate the protective effects of α-tocopherol and zinc sulfate on oxidative stress parameters in ovarian granulosa cells of female Wistar rats (Rattus norvegicus) exposed to lead acetate (Pb(C₂H₃O₂)₂). A total of 25 rats were randomly divided into five groups: Control (C), lead exposure only (T0), α-tocopherol + lead (T1), zinc sulfate + lead (T2), and α-tocopherol + zinc sulfate + lead (T3). Treatments were administered orally for 21 days. Superoxide dismutase (SOD) levels were measured spectrophotometrically, and p53 expression was analyzed using immunohistochemistry. The results showed that SOD levels significantly decreased in group T0 compared to the control (p <0.05), while treatment with either α-tocopherol (T1), zinc sulfate (T2), or their combination (T3) significantly improved SOD levels compared to T0. Moreover, p53 expression was markedly elevated in T0, indicating enhanced oxidative stress and potential apoptosis, whereas all antioxidant-treated groups showed reduced p53 expression, with T3 demonstrating values comparable to the control group. In conclusion, the combination of α-tocopherol and zinc sulfate provided a synergistic antioxidant effect, effectively enhancing SOD levels and suppressing p53 expression in granulosa cells of lead-exposed rats. This suggested a promising therapeutic potential of these compounds in mitigating lead-induced ovarian toxicity.

Incidence and associated risk factors of uterine prolapse in dairy cows in Manoharganj Upazila, Cumilla District, Bangladesh

Al Wasef — 2025-04-01

Uterine prolapse is a medical emergency in cows that can be life-threatening if not promptly treated. This study aimed to determine the incidence, associated risk factors, survival rate, conception rate, and management practices related to uterine prolapse in dairy cows in Manoharganj Upazila, Cumilla District, Bangladesh. Data collected from cow owners were analyzed using the Chi-square test. A total of 102 cases of uterine prolapse were manually corrected. The incidence was found to be higher in crossbred (61.76%) compared to local (38.24%) cows. Similarly, cows that were artificially inseminated showed a significantly higher incidence (93.14%) than those bred naturally (6.86%). Pluriparous cows had a greater incidence (77.45%) than primiparous cows (22.55%). Additionally, cows with a previous history of vaginal prolapse (48.04%) or dystocia (59.80%) were more likely to experience uterine prolapse than those without such histories. Following treatment, 91.18% of the affected cows survived, while 8.82% died. Among the survivors, 15.05% were culled, and the remaining cows were artificially inseminated of which 82.27% successfully conceived. The study also observed that 3.92% of cows were diagnosed with hypocalcemia shortly after prolapse correction. These findings highlight the importance of immediate veterinary intervention and the need for proper nutritional management during pregnancy to help prevent uterine prolapse.

Impact of different antibiotic treatments on pregnancy maintenance in Holstein Friesian cows infected with foot-and-mouth disease

Vensko Zico Dandi Wahyu Pratama — 2025-04-01

This study was conducted at Setia Kawan dairy cattle farming cooperative in Nongkojajar, Pasuruan, East Java to evaluate the effects of different antibiotic treatments on pregnancy sustainability in Holstein Friesian (HF) dairy cows infected with foot and mouth disease (FMD). The objective was to identify which antibiotics have minimal impact on pregnancy outcomes in FMD infected cows. A total of 106 pregnant HF cows infected with FMD were included in this study. Samples were selected using a purposive sampling method with inclusion criteria of pregnant HF dairy cows that tested positive for FMD. Four types of antibiotics were assessed, including enrofloxacin, oxytetracycline, amoxicillin, and penicillin-streptomycin. The results showed that among cows treated with enrofloxacin, 88.46% maintained pregnancy while 11.54% experienced abortion. For oxytetracycline, 73.08% of cows did not abort, whereas 26.92% did. In the amoxicillin group, 61.54% maintained pregnancy and 38.46% aborted. For penicillin-streptomycin, only limited data were available, with 23.07% maintaining pregnancy and no recorded cases of abortion. In conclusion, while variations were observed among different antibiotic treatments, statistical analysis indicated no significant association between antibiotic type and abortion rate in HF cows infected with FMD. Further studies with larger sample sizes are recommended to confirm these findings.