Ovozoa : Journal of Animal Reproduction https://e-journal.unair.ac.id/OVZ <p>OVOZOA: Journal of Animal Reproduction (OVOZOA J AnimReprod) <a href="https://issn.lipi.go.id/terbit/detail/1350359151" target="_blank">p-ISSN: 2302-6464</a>; <a href="https://issn.lipi.go.id/terbit/detail/1589875259" target="_self">e-ISSN: 2722-967X</a> is published periodically three times a year (April, August, and December) by the Department of Veterinary Reproduction Faculty of Veterinary Medicine Universitas Airlangga in collaboration with <a href="https://drive.google.com/file/d/170iveGZDhFlvaom0U1dFfOVwmpB1fZBP/view?usp=sharing" target="_blank">the Indonesian Association of Department of Veterinary Reproduction</a>. Since March 27, 2021 OVOZOA Journal of Animal Reproduction has also collaborated with <a href="https://drive.google.com/file/d/1zZJGAEaG0yq_vRFb9Q7S2MUjPvy2QrUO/view?usp=sharing" target="_blank">the Indonesian Animal Reproduction Association</a></p><p><strong>Focus and Scope</strong></p><p>This journal published original articles, review articles, and case studies in Indonesian or English, in the scope of veterinary, animal, and biotechnology of reproduction from researchers, lecturers, students, and other practitioners around Indonesia and worldwide.</p><p><strong>Announcement</strong><br />Starting in 2020 OVOZOA Journal of Animal Reproduction is published three times per year (April, August, and December)</p><p> </p> en-US <p>Journal Ovozoa by <a href="http://www.unair.ac.id/" rel="cc:attributionURL">Unair</a> is licensed under a <a href="http://creativecommons.org/licenses/by-sa/4.0/" rel="license">Creative Commons Attribution-ShareAlike 4.0 International License</a>.</p><p align="justify">1. The journal allows <span class="m_-8872622167488361851m_3889253648079045002m_3801934354951983127m_-2782718132241447849m_-7691471417709598651m_7256872056212528454m_3794665997207553305gmail-animated">the author to hold the copyright of the article without restrictions</span>.</p><p align="justify">2. The journal allows the author(s) to retain publishing rights without restrictions</p><p align="justify">3. The legal formal aspect of journal publication accessibility refers to Creative Commons Attribution Share-Alike (CC BY-SA).</p><p align="justify">4. The Creative Commons Attribution Share-Alike (CC BY-SA) license allows re-distribution and re-use of a licensed work on the conditions that the creator is appropriately credited and that any derivative work is made available under “the same, similar or a compatible license”. Other than the conditions mentioned above, the editorial board is not responsible for copyright violation.</p> imam.mustofa@fkh.unair.ac.id (Prof. Dr. Imam Mustofa) agil.ramadhan.achmad@staf.unair.ac.id (Agil Ramadhan) Mon, 11 Apr 2022 00:00:00 +0000 OJS 3.3.0.10 http://blogs.law.harvard.edu/tech/rss 60 Uterine torsion in Simmental crossbreed cow https://e-journal.unair.ac.id/OVZ/article/view/32407 <p>This case report describes the treatment of uterine torsion in Simmental crossbreed cattle. The dam was five years old, twice parity, in nine months and ten days pregnancy. Based on the anamnesis, it is known that the cows show signs of restlessness, straining, more robust by the time. However, there was no visible edema in the vulva, no ruptured membranes, no legs and head of the fetus coming out of the dam's vulva. Tretment was carried out by traditional rolling until the maximum uterine lumen opening was obtained. The position of the fetus is head flexion posture, so to remove it vaginally, the head is repositioned first and then forced to pull it out until the fetus comes out. Post-treatment, the dam's condition was still weak, unable to stand up, did not want to eat, and only drank a little. The drugs given were analgesics-antipyretics (repeated in the afternoon), long-acting antibiotics, vitamin B1, and vitamins and minerals mixed. After a few hours, the dam was up and walking around, feeding and drinking, and the placenta completely separated. The fetus's condition was alive, female sex, weak condition, the condition improves after being given milk from the dam. It can be concluded that the case of uterine torsion in Simmental crossbreed cows has been successfully treated 24 hours after the signs of birth using the traditional rolling technique, and the fetus can be expelled vaginally. The condition of the dam and fetus after the treatment of uterine were torsion gradually became healthy.<em></em></p> Sri Rahayu Copyright (c) 2022 Sri Rahayu http://creativecommons.org/licenses/by-sa/4.0 https://e-journal.unair.ac.id/OVZ/article/view/32407 Mon, 11 Apr 2022 00:00:00 +0000 The addition of vitamin C in tris–egg yolk extender maintained Sapera goat semen quality in 5° C storage https://e-journal.unair.ac.id/OVZ/article/view/32287 <p>Goats are small ruminants that are reared by the rural community for financial income and nutrition. This study was aimed to determine the effect of vitamin C addition in tris–egg yolk extender on the lifespan of Sapera goat spermatozoa stored in 5<sup>o</sup> C. Semen was collected from 1.5 years old Sapera male goat. The ejaculates were diluted in Tris-egg yolk (T-EY) extender added with 0, 0.2, 0.3, and 0.4 g vitamin C/mL extender for T0, T1, T2, and T3 groups, respectively. The spermatozoa motility, viability, and morphological abnormality were assessed along with storage at 5° C. The result showed that spermatozoa motility was the highest (p &lt;0.05) in T1 compared to other groups and qualified for AI use for up to 72 hours. In 24 hours storage, the spermatozoa viability was not significantly different (p &gt;0.05) among the groups. The spermatozoa viability in the T1 group was the highest (p &lt;0.05) among the groups along 48-96 hours of storage. The spermatozoa morphological abnormalities of the T1 group was the lowest (p &lt;0.05) compared to other treatment groups in the range of 24-72 hour storage. In the control group (T0), the less than 5% spermatozoa morphological abnormalities (qualified for artificial insemination) were only in the 24 hours storage, while those of the T1 group were up to 72 hours. It could be concluded that the addition of 0.2 g vitamin C/100 mL T-EY extender maintained the quality of Sapera goat semen for 72 hours at 5°C.</p> Jolly Rifqi Pahlevy, Hermin Ratnani, Tjuk Imam Restiadi, Faisal Fikri, Amung Logam Saputro, Bodhi Agustono Copyright (c) 2022 Jolly Rifqi Pahlevy, Hermin Ratnani, Tjuk Imam Restiadi, Faisal Fikri, Amung Logam Saputro, Bodhi Agustono http://creativecommons.org/licenses/by-sa/4.0 https://e-journal.unair.ac.id/OVZ/article/view/32287 Mon, 11 Apr 2022 00:00:00 +0000 Correlation of parity and age to services per conception, conception rate, and gestation length in Holstein Friesian cross cows https://e-journal.unair.ac.id/OVZ/article/view/32477 <p>Small farmers dominate dairy farming in Indonesia, and the average productivity is low. The contribution of domestic production only reaches about 23% of the total national milk consumption needs as a source of human nutrition. This study was aimed to identify the relationship between cow's parity and age to services per conception (S/C), conception rate (CR), and gestation length (GL) of Holstein Friesian (HF) cross dairy cows. Data of this study consisted of primary data obtained from farmers interview, and secondary data from the Cooperative Unit of Argopuro village, Krucil, Probolinggo. Sample size was 200 cows, which was 10% of the total population. Data were analyzed for correlation and linear regression using the Statistic Package and Service Solution (SPSS) software version 20 (IBM Corp., USA) at a 95% confidence level. The average S/C, CR, and GL were 2.55 ± 1.57, 21%, and 278.19 ± 11.92 days, respectively. There was no significant correlation (p &gt;0.05) between the parity with S/C, CR, and GL and the age with CR and GL. The only significant correlation (p &lt;0.05) was between the age and S/C. Regression of S/C based on age followed the equation S/C = 0.104 + 0.407*age, with a correlation coefficient and determination coefficient of 0.733 and 0.538, respectively. It could be concluded that S/C could be predicted based on the age of the cows.</p> Norma Amalia Ulfah, Abdul Samik, Mas’ud Hariadi, Tri Wahyu Suprayogi, Sri Hidanah Copyright (c) 2022 Norma Amalia Ulfah, Abdul Samik, Mas’ud Hariadi, Tri Wahyu Suprayogi, Sri Hidanah http://creativecommons.org/licenses/by-sa/4.0 https://e-journal.unair.ac.id/OVZ/article/view/32477 Mon, 11 Apr 2022 00:00:00 +0000 Moringa oleifera leaf extract restored the diameter and epithelium thickness of the seminiferous tubules of rat (Rattus norvegicus) injected with gentamicin https://e-journal.unair.ac.id/OVZ/article/view/31041 <p>This research aims to determine the effect of <em>Moringa oleifera</em> leaf extract (MLE) on the diameter and epithelium thickness of the seminiferous tubules of rats induced with gentamicin. This study used 25 male rats (<em>Rattus norvegicus) </em>aged 8-12 weeks with 150-200 grams body weight. Negative control group (C-) was injected with aquadest and given 0.5% Na-CMC orally, positive control group (C+) was induced with gentamicin injection of 5 mg/kg BW/day and given 0.5% Na-CMC orally, while groups T1, T2, and T3 were induced with gentamicin at the same dose and treated orally with MLE of 200, 316, 500 mg/kg BW/day. Analysis of variance showed significant differences and further analysis using Duncan Multiple Range Test showed difference in each group. The results showed that treatment with MLE of 500 mg/kg BW/day improved the diameter and epithelium thickness of the seminiferous tubules of rats injected with gentamicin. It could be concluded that MLE restored the diameter and epithelium thickness of the seminiferous tubules of rats induced with gentamicin.</p> Meidy Mardatillah, Wurlina Wurlina, Ira Sari Yudaniayanti, Hardany Primarizky, Hani Plumeriastuti, Iwan Sahrial Hamid Copyright (c) 2022 Meidy Mardatillah, Wurlina Wurlina, Ira Sari Yudaniayanti, Hardany Primarizky, Hani Plumeriastuti, Iwan Sahrial Hamid http://creativecommons.org/licenses/by-sa/4.0 https://e-journal.unair.ac.id/OVZ/article/view/31041 Mon, 11 Apr 2022 00:00:00 +0000 pH and ferning score of cervical mucus related to gestation and calf gender of Holstein Friesian crossbreed cows https://e-journal.unair.ac.id/OVZ/article/view/34075 <p>This study aimed to compare the ferning score based on the pH of the cervical mucus and its relation with gestation and the calf gender of cows. Ten Holstein Friesian crossbreed cows which were detected in estrus and about to be artificially inseminated, were used for this study. Sample of cervical mucus was collected using Metricheck for pH and ferning observations. Pregnancy diagnosis was conducted two months after insemination, and the gender of the calves was recorded at parturition. This study indicated that cervical mucus pH did not affect the ferning characteristics, ferning score, and pregnancy of cows. However, higher ferning scores tended to result in the pregnancy of cows. Also, alkaline pH tended to result in male calves and vice versa. Further study is needed with a more significant number of samples related to the application of ferning display as an indicator of estrus to obtain a higher pregnancy rate.</p> Muhammad Billy Ferdiansyah, Iwan Sahrial Hamid, Herry Agus Hermadi, Abdul Samik, Tatik Hernawati Copyright (c) 2022 Muhammad Billy Ferdiansyah, Iwan Sahrial, Herry Agus Hermadi, Abdul Samik, Tatik Hernawati http://creativecommons.org/licenses/by-sa/4.0 https://e-journal.unair.ac.id/OVZ/article/view/34075 Mon, 11 Apr 2022 00:00:00 +0000 Pre-freezing at 10 cm above liquid nitrogen surface for eight minutes resulted the best Sapera goat semen quality https://e-journal.unair.ac.id/OVZ/article/view/32289 <p><em>Sapera goat is a dairy goat resulting from a cross between a Saanen buck and an </em>Ettawah cross doe. These small ruminants are reared by rural households for poverty reduction and undernutrition prevention. Breeding of Sapera goat through artificial insemination (AI) technique was expected to accelerate the increase in population. Unfortunately, the frozen semen of Sapera buck is not available yet. This study aims to determine the effect of the distance between semen straw and liquid nitrogen surface during pre-freezing process on the quality of frozen semen of Sapera goats. Semen was obtained from 1.5 years old Sapera buck. The ejaculates were diluted in tris-egg yolk extender to obtain a concentration of 120 x 10<sup>6</sup> spermatozoa/mL, then equilibrated at 5°C for one hour. The extended semen was packaged in 0.5 mL French straws (60 x 10<sup>6</sup> spermatozoa/straw). The filled straws were pre-frozen in the cold handling cabinet (Minitube, Germany) held at 10, 11, 12, 13, 14, and 15 cm above liquid nitrogen respectively for T1, T2, T3, T4, T5, and T6 groups, each with four replicates. After pre-freezing for eight minutes the straws were immediately plunged, and stored in liquid nitrogen (-196°C) for two days, followed by quality assessment. The best post-thawed motility (41.75 ± 2.06 %), viability (49.00 ± 0.82 %) and morphological abnormalities (4.75 ± 0.96 %) were obtained from pre-freezing stage with a distance of 10 cm. It could be concluded that in tris-egg yolk extender, post-thawed spermatozoa viability and motility of Sapera buck were qualified for AI when fresh semen was pre-frozen at 10 cm distance of straws from the surface of liquid nitrogen for eight minutes.</p><p align="center"> </p> Zehansyah Algifari Pasha, Bodhi Agustono, Amung Logam Saputro Copyright (c) 2022 Zehansyah Algifari Pasha, Bodhi Agustono , Amung Logam Saputro http://creativecommons.org/licenses/by-sa/4.0 https://e-journal.unair.ac.id/OVZ/article/view/32289 Mon, 11 Apr 2022 00:00:00 +0000 Virgin coconut oil protected the diameter and the epithelium thickness of seminiferous tubules of mice (Mus musculus) from oral ethanol induction https://e-journal.unair.ac.id/OVZ/article/view/30831 <p>This study investigated the preventive effect of virgin coconut oil (VCO) on the epithelium thickness and the diameter of the seminiferous tubules induced by ethanol in mice (<em>Mus musculus</em>). This study used 20 male mice as the experimental animal which were divided into five groups with four mice in each group. Negative control (C-) was given 2% Tween and aquadest, while positive control (C+) was given 2% Tween and 33% ethanol. T1, T2, and T3 were respectively given 0.09, 0.19, and 0.37 mL/kg bw VCO and 33% ethanol (0.2 mL/kg bw). VCO was given orally for 39 days, and ethanol was given orally seven days later for 32 days. Ethanol was administered two hours after the VCO administration. Analysis of variance followed by Tukey’s range test on the epithelium thickness and the diameter of the seminiferous tubules showed significant differences (p &lt;0.05) of C+ group from the other groups. Whereas, there was no significant difference (p &gt;0.05) was found among C-, T1, T2 and T3 group. The result concluded that VCO could protect the testis of mice from the damage caused by ethanol.</p> Vishantheni S Ravi, Soeharsono Soeharsono, Hani Plumeriastuti, Kadek Rachmawati Copyright (c) 2022 Vishantheni S Ravi, Soeharsono Soeharsono , Hani Plumeriastuti, Kadek Rachmawati http://creativecommons.org/licenses/by-sa/4.0 https://e-journal.unair.ac.id/OVZ/article/view/30831 Mon, 11 Apr 2022 00:00:00 +0000