Synergistic effect of the combination of Cinnamomum burmanii, vigna unguiculata, and papain exracts derived from carica papaya latex against C. albicans biofilms degradation

Muhammad Luthfi, Indah Listiana Kriswandini, Fitriah Hasan Zaba

= http://dx.doi.org/10.20473/j.djmkg.v49.i2.p71-75
Abstract views = 288 times | views = 197 times

Abstract


Background: Candidiasis is an opportunistic infection commonly occurs on host with immunodeficiency, organ transplantation, leukopenia, or radiation therapy. Biofilms are structures that protect C. albicans from antifungals treatments. C. albicans biofilms display multidrug resistance to antifungal agents. Purpose: This study aimed to know whether the combination of Cinnamomum burmannii, Vigna unguiculata, and Papain extracts derived from Carica papaya latex has inadequate inhibitory effects against C.albicans biofilms compared to the combination of Cinnamomum burmannii and Vigna unguiculata extracts. Method: C. albicans. growing on SDA were dissolved in 1 McFarland of sterile aquadest. Micro-plate was filled with 180 µL of SDB, glucose 8%, and 20 µL of C. albicans. Suspension was incubated at 37oC overnight. Extracts were added and incubated for 24 hours. Then, each well was washed with distilled water, and stained with crystal violet 0.1% for 15 minutes. Afterward, each well was washed with distilled water and immediately stained with acetic acid. After 15 minutes of staining, the suspension was transferred to a new well, then measured with micro-plate reader at 595 nm. Results: The combination of Cinnamomum burmanii and Vigna unguiculata extracts had adequate inhibitory effects which is equal to 60.75%. Inhibition increased to 72.09%, 79.06%, and 79.50% after Papain derived from Carica papaya latex was added on concentrations of 138 mg/mL, 276 mg/mL, and 552 mg/mL. Conclusion: The combination of Cinnamomum burmanii (0.25µg/mL), Vigna unguiculata (200 µg/mL), and Papain (276 µg/mL) extracts showed an optimum synergic inhibition for C. albicans biofilms.

Keywords


C. albicans biofilm; Cinnamomum burmannii; Vigna unguiculata; Papain

Full Text:

PDF

References


Darouiche RO, Mansouri MD, Kojic EM. Antifungal activity of antimicrobial-impregnated devices. Clin Microbiol Infect 2006; 12(4): 397-9.

Richard ML, Nobile CJ, Bruno VM, Mitchell AP. Candida albicans biofilm defective mutants. Eukaryot Cell 2005; 4(8): 1493–502.

McCullough MJ, Savage NW. Oral candidosis and the therapeutic use of antifungal agents in dentistry. Aust Dent J 2005; 50(4 Suppl 2): S36-9.

Nett J, Lincoln L, Marchillo K, Massey R, Holoyda K, Hoff B, VanHandel M, Andes D. Putative role of beta-1,3 glucans in Candida albicans biofilm resistance. Antimicrob Agents Chemother 2007; 51(2): 510-20.

Krishna KL, Paridhavi M, Patel JA. Review on nutritional, medicinal and pharmacologinal properties of papaya (Carica papaya Linn.). Natural Product Radiance 2008; 7(4): 364-73.

Giordani R, Siepaio M, Moulin-Traffort J, Régli P. Antifungal action of Carica papaya latex: isolation of fungal cell wall hydrolysing enzymes. Mycoses 1991; 34(11-12): 469-77.

Oliveira JTA, Barreto ALH, Vasconcelos IM, Eloy YRG, Gondim DMF, Fernandes CF, Freire-Filho FR. Role of antioxidant enzymes, hydrogen peroxide and PR-proteins in the compatible and incompatible interactions of Cowpea (Vigna unguiculata) genotypes with the Fungus Colletotrichum gloeosporioides. J Plant Physiol Pathol 2014; 2(3):2-8.

Erna F, Rostiny, Sherman S. Efektivitas minyak kayu manis dalam menghambat pertumbuhan koloni Candida albicans pada resin akrilik. Journal of Prosthodontic 2010; 11(2): 19-23.

O’Toole, GA. Microtiter dish biofilm formation assay. J Vis Exp 2011; (47) pii: 2437.

Mahmoudabadi AZ, Zarrin M, Kiasat N. Biofilm formation and susceptibility to amphotericin B and fluconazole in Candida albicans. J Microbiol 2014; 7(7): e17105.

Bakkiyaraj D, Nandhini JR, Malathy B, Pandian SK. The anti-biofilm potential of pomegranate (Punica granatum L.) extract against human bacterial and fungal pathogens. Biofouling 2013; 29(8): 929-37.

Kao LS, Green C. Analysis of variance: is there a difference in means and what does it mean?. J Surg Res 2008; 144(1): 158-70.

El-Katatny MH, Somitsch W, Robra KH, EI-Katatny MS, Gübitz GM. Production of chitinase and β-1,3-glucanase by Trichoderma harzianum for control of the Phytopathogenic Fungus Sclerotium rolfsii. J Food Technol Biotechnol 2000; 38(3): 170-80.

Raja MRC, Srinivasan V, Selvaraj S, Mahapatra SK. Versatile and synergistic potential of eugenol: a review. Pharm Anal Acta 2015; 6(5): 367.

Dongoran DS. Pengaruh aktivator sistein dan natrium klorida terhadap aktivitas papain. Jurnal Sains Kimia 2004; 8(1): 30-5.

Nikaido H. Multidrug resistance in bacteria. Annu Rev Biochem 2009; 78: 119-46.

Omeje KO, Eze SO, Ozougwu VE, Ubani CS, Osayi E, Onyeke CC, Chilaka FC. Application of papain from paw paw (Carica papaya) latex in the hydrolysis of tiger nut (C.esculentus) proteins. Mitteilungen Klosterneuburg 2014; 64: 1-17.


Refbacks

  • There are currently no refbacks.


View My Stats