The thickness of odontoblast-like cell layer after induced by propolis extract and calcium hydroxide

Irfan Dwiandhono, Ruslan Effendy, Sri Kunarti

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Background: Propolis is a substance made from resin collected by bees (Apis mellifera) from variety of plants, mixed with its saliva and various enzymes to build a nest. Propolis has potential antimicrobial and antiinflammatory agents with some advantages over calcium hydroxide (Ca(OH)2). Ca(OH)2 has been considered as the “gold standard” of direct pulp-capping materials, but there are still some weakness of its application. First, it can induce pulp inflammation which last up to 3 months. Second, the tissue response to Ca(OH)2 is not always predictable. Third, the tunnel defect can probably formed in dentinal bridge with possible bacterial invasion in that gap. Purpose: This study was aimed to determine and compare the thickness of odontoblast-like cells layer after induced by propolis extract and Ca(OH)2 in rat’s pulp tissue. Method: Class 1 preparation was done in maxillary first molar tooth of wistar mice until the pulp opened. The Ca(OH)2 and propolis extract was applied to induce the formation of odontoblast-like cells, the cavity was filled with RMGIC. The teeth were extracted (after 14 and 28 days of induction). The samples were then processed for histological evaluation. Result: There were significant differences between the thickness of odontoblast-like cells after induced by propolis extract and Ca(OH)2. Conclusion: The propolis extract as the direct pulp capping agent produces thicker odontoblast-like cell layer compared to Ca(OH)2.


odontoblast-like cells; propolis; calcium hydroxide

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Torabinejad M, Walton RE. Endodontics: principles and practice. 4th ed. Missouri: Saunders; 2009. p. 9-14.

Hargreaves KM, Berman LH, Cohen S. Cohen’s pathways of the pulp expert consult. 10th ed. St. Louis: Mosby; 2011. p. 490, 518, 626, 812.

Willershausen B, Willershausen I, Ross A, Velikonja S, Kasaj A, Blettner M. Retrospective study on direct pulp capping with calcium hydroxide. Quintessence Int 2011; 42(2): 165-71.

Janebodin K, Horst OV, Osathanon T. Dental pulp response to pulp capping materials and bioactive molecules. CU Dent J 2010; 33: 229-48.

Bankova V, Castro SD, Marcucci M. Propolis: recent advances in chemistry and plant origin. Apidologie, Springer Verlag 2000; 31(1): 3–15.

Almas K, Dahlan A, Mahmoud A. Propolis as a natural remedy: an update. Saudi Dent J 2001; 13(1): 45-9.

Sabir A, Tabbu CR, Agustiono P, Sosroseno W. Histological analysis of rat dental pulp tissue capped with propolis. J Oral Sci 2005; 47(3): 135-8.

Parolia A, Thomas MS, Kundabala M, Mohan M. Propolis and its potential uses in oral health. Int J Med Med Sci 2010; 2(7): 210-5.

Asgary S, Eghbal MJ, Parirokh M, Ghanavati F, Rahimi H. A comparative study of histologic response to different pulp capping materials and a novel endodontic cement. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2008; 106(4): 609-14.

Tziafas D, Smith AJ, Lesot H. Designing new treatment strategies in vital pulp therapy. J Dent 2000; 28(2): 77-92.

Hargreaves KM, Goodis HE. Seltzer and Bender’s dental pulp. Hanover Park: Quintessence Publishing Co, Inc; 2002. p. 247-79.

About I, Bottero MJ, de Denato P, Camps J, Franquin JC, Mitsiadis TA. Human dentin production in vitro. Exp Cell Res 2000; 258(1): 33-41.

Sloan AJ, Perry H, Matthews JB, Smith AJ. Transforming growth factor-beta isoform expression in mature human healthy and carious molar teeth. Histochem J 2000; 32(4): 247-52.

Shirakawa M, Shiba H, Nakanishi K, Ogawa T, Okamoto H, Noshiro M, Kato Y. Transforming growth factor-beta-1 reduces alkaline phosphatase mRNA and activity and stimulates cell proliferation in cultures of human pulp cells. J Dent Res 1994; 73(9): 1509-14.

Widjiastuti I, Yuanita T, Hutagalung J, Rukmo M, Safitri I. 2014. East java propolis inhibits cytokine pro-inflammatory in odontoblast-like cells human pulp. Journal of Agricultural Science and Technology A 2014; 4(1): 27-32.

Kunarti S. Pulp tissue inflammation and angiogenesis after pulp capping with transforming growth factor β1. Dent J (Majalah Kedokteran Gigi) 2008; 41(2): 88-90.

Murray PE, Windsor LJ, Smyth TW, Hafez AA, Cox CF. Analysis of pulpal reactions to restorative procedures, material, pulp capping, and future therapies. Crit Rev Oral Biol Med 2002; 13(6): 509-20.

Yatim W. Biologi sel lanjut. Bandung: Tarsito; 2003. p. 1-84.

Goldberg M, Smith AJ. Cell and extracellular matrices of dentin and pulp: a biological basis for repair and tissue engineering. Crit Rev Oral Biol Med 2004; 15(1): 13-27.

Ahangari Z, Naseri M, Jalili M, Mansouri Y, Mashhadiabbas F, Torkaman A. Effect of propolis on dentin regeneration and the potential role of dental pulp stem cell in Guinea pigs. Cell J 2012; 13(4): 223-8.

Kumar, Abbas, Fausto. Robbins & Cotran Dasar Patologis Penyakit. 7th ed. Jakarta: EGC; 2010. p. 101.


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