The effect of combined Moringa oleifera and demineralized freeze-dried bovine bone xenograft on the amount of osteoblast and osteoclast in the healing of tooth extraction socket of Cavia cobaya

Rostiny Rostiny, Eha Djulaeha, Nike Hendrijantini, Agus Pudijanto

= http://dx.doi.org/10.20473/j.djmkg.v49.i1.p37-42
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Abstract


Background: Alveolar bone has an important role in providing support to teeth and dentures. Loss of support caused by alveolar resorption will cause functional and aesthetic problems. Preservation socket using bone graft is one way to maintain the dimensions of the alveolar bone. Moringa oleifera leaf can increase the activity of bone graft in the formation of new bone. Purpose: This study was aimed to evaluate the effect of combined Moringa oleivera leaf extract and demineralized freeze-dried bovine bone xenograft (DFDBBX) towards the formation of osteoblasts and osteoclasts in the tooth extraction sockets of cavia cobaya. Method: This study used 28 cavia cobayas divided into four groups. The combination of Moringa oleifera leaf extract and DFDBBX was inducted into the sockets of lower incisor tooth with certain dose in each group, ointment 1 containing PEG (a mixture of PEG 400 and PEG 4000) for control group, ointment 2 containing Moringa oleifera leaf extract and DFDBBX and PEG (at active subtance consentration of 0.5%) for group 1, ointment 3 containing Moringa oleifera leaf extract and DFDBBX and PEG (at active substance concentration of 1%) for group 2, and Ointment 4 containing Moringa oleifera leaf extract and DFDBBX and PEG (at active substance consentration of 2%) for group 3. paraffin block preparations were made for histopathology examination using hematoxylin eosin staining. Result: The results showed that there were significant differences of the number of osteoblasts and osteoclasts in each treatment group (p <0.05). Conclusion: It can be concluded that the combination of Moringa oleifera leaf extract and DFDBBX at 2% cocentration can increase the number of osteoblasts and decrease osteoclasts in the healing of tooth extraction sockets of cavia cobaya.

Keywords


demineralized freeze-dried bovine bone xenograft; Moringa oleifera leaf; osteoblast; osteoclast

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References


Allegrini S Jr, Koening B Jr, Allegrini MR, Yoshimoto M, Gedrange T, Fanghaenel J, Lipski M. Alveolar ridge sockets preservation with bone grafting–Review. Ann Acad Med Stetin 2008; 54(1): 70–81.

Irinakis T. Rationale for socket preservation after extraction of a single-rooted tooth when planning for future implant placement. J Can Dent Assoc 2007; 72(10): 917–22.

Nevins M, Camelo M, De Paoli S, Friedland B, Schenk RK, ParmaBenfenati S, Simion M, Tinti C, Wagenberg B. A study of the buccal wall of extraction socket of teeth with prominent roots. Int J Periodontics Restorative Dent 2006; 26(1): 19-20.

Oghli AA, Steveling. Ridge preservation following tooth extraction: A comparison between a traumatic extraction and socket seal surgery. Quint Int 2010; 4(7): 605-9.

Gark K, Lynch SE, Robert G, Robert EM. Tissue engineering applications in maxillofacial surgery and periodontics. Illinois: Quintessence Public Inc; 1999. p. 83-9.

Weiss CM, Weis A. Principle and practice of implant dentistry. 1st ed. United State of America: Mosby Inc; 2001. p. 659.

Baharuddin NA, Kamin S, Samsuddin AR. The use of demineralized freeze-dried bovine bone xenograft in reducing post surgical periodontal pocket depth. Annals of Dentistry. University of Malaya 2003; 10: 33-6.

Nevins M, Camelo M, De Angelis N, Hanratty JJ, Khang WG, Kwon JJ, Rasperini G, Rocchietta I, Schupbach P, Kim DM. The clinical and histological efficacy of xenograft granules for maxillary sinus floor augmentation. Int J Periodontics Restorative Dent 2011; 31(3): 227-35.

Copin JA. Study of the nutritional and medicinal values of Moringa oleifera leaves from sub-saharan Africa: Ghana, Rwanda Senegal and Zambia.Thesis. The State University of New Jersey: Graduate School-New Brunswick Rutgers; 2008. p. 5-99.

Kasolo JN, Bimenya GS, Ojok L, Ochieng J, Ognel JW. Phytochemical and uses of Moringa oleifera leaves in Ugandan rural communities. Journal of Medicinal Plants Research 2010; 4(9): 753-7.

Patel C, Rangrez A, Parikh P. The anti-osteoporotic effect of Moringa oleifera on osteoblastic cells: SaOS2. Journal of Pharmacy and Biological Sciences 2013; 5(2): 10-7.

Coppin JW, Xu Y, Chen H, Pan MH, Ho CT, Juliani R, Simon JE, Wu. Determination of flavonoid by LC/MS anti inflammatory activity in Moringa oleifera. Journal of Functional Foods 2013; 5: 1892-9.

Araújo LC, Aguiar JS, Napoleão TH, Mota FV, Barros AL, Moura MC, Coriolano MC, Coelho LC, Silva TG, Paiva PM. Evaluation of cytotoxic and anti-inflammatory activities of extracts and lectins of Moringa oleifera seeds. PLoS One 2013; 8(12): e81973.

Weijen FV, Actqua D, Slot DE. Alveolar bone dimensional changes of post-extraction sockets in humans: a systematic review. J Clin Periodontol 2009; 36(12): 1048-58.

Yustina AR, Suardita K, Agustin D. Peningkatan jumlah osteoklas pada keradangan periapikal akibat induksi lipopolisakarida porphyromonas gingivalis (Suatu penelitian laboratories menggunakan tikus). Journal Biosains 2012; 14(3): 140-4.

Ghom A, Mhaske S. Textbook of oral phatology.1st ed. New Delhi, India: Jaypee Brothers Medical Publisher Ltd; 2009. p. 86-7.

Rajendran S. Textbook of oral phatology. 6th ed. India: Elseiver; 2010. p. 599-60.

Kresnoadi U, Rahayu RP. The influence of Aloe vera BNP 2 expression and amount of osteoblast of alveolar bone induced into tooth extraction socket (Cavia Cobaya). Journal of Biological Researches 2014; 19(2): 2-10.

Vindani D. Efektivitas kombinasi ekstrak Jinten Hitam (Nigella Sativa) dan Graft terhadap peningkatan osteoblas tulang alveol pada Cavia Cobaya. Thesis. Surabaya: Pendidikan Dokter Gigi Spesialis Universitas Airlangga; 2013. p. 24-35.

Jiang Yang Guo, Chiyan Choi, Yuzhong Zheng, Ping Chen, Tingxia Dong, Zheng-Tao Wang, Günter Vollmer, Taiwai Lau, Wah-keung Tsim. Kaempferol as a flavonoid induces osteoblastic differentiation via estrogen receptor signaling. Chinese Medicine 2012; 7(10): 1-7.

Yamaguchi M. The osteogenic effect of bioactive flavonoid p-hydroxycinnamic acid: development in osteoporosis treatment. OA Biotechnology 2013; 01: 2(2): 15.

Jiaying G. Mechanistic studies on the osteogenic effects of dietary flavonoids in cultured osteoblasts: potential drug development against osteoporosis. Thesis. Hong Kong: Hong Kong University of Science and Technology; 2011. p. 146-50.

Thomas SDC. Bone turnover markers. Aust Prescr 2012; 35: 156-8.

Yudaniayanti IS. Aktifitas alkaline phosphatase pada proses kesembuhan patah tulang femur dengan terapi CaCO3 dosis tinggi pada tikus jantan. Media Kedokteran Hewan 2008; 21(1): 15-8.

Gupta R, Pandit N, Malik R, Sood S. Clinical and radiological evaluation of an osseous xenograft for the treatment of infrabony defects. J Can Dent Assoc 2007; 73(6): 513.

Baharuddin NA, Kamin S, Samsuddin AR. The original article use of demineralized freeze-dried bovine bone xenograft in reducing post-surgical periodontal recession. Annals of Dentistry. University of Malaya 2005; 12: 37–40.

Purnomo A, Adji D. Ekspresi bone morphogenetic protein-2 untuk mengukur efektivitas biomaterial freeze dried bovine bone xenograft (FDBBX) sebagai bahan penyambung tulang. Jurnal Sain Veteriner 2012; 30(1): 1-6.

Khan SN, Cammisa FP Jr, Sandhu HS, Diwan AD, Girardi FP, Lane JM. The biology of bone grafting. J Am Acad Orthop Surg 2005; 13(1): 77-86.

Takanyangi. Ostoimunology: Share mechanism and crosstalk between the immunes and bone system. 7th ed. Tokyo: Nature Publishing Group; 2007. p. 292-302.

Sreelatha S, Jeyachitra A, Padma PR. Antiproliferation and induction of apoptosis by Moringa oleifera leaf extract on human cancer cells. Food Chem Toxicol 2011; 49(6): 1270-5.

Thorne research. 2000. Alternative medecine review. Vol.3(2). Available from: www.thorne.com/altmedrev/.fulltex/3/2/140.pdf. Accessed on 10 may 2015.

Della L. Anti inflammatory activity of benzopyrones that are inhibitors of cyclo and lipo-oxygenase. Pharmacological Ressearch Communications 2001; 20: 91-4.

Amijaya APP, Murwani S, Wardhana AW. 2012. Efek ekstrak air daun kelor (Moringa Oleifera) terhadap kadar tumor necrosis factor Alpha (TNFα) dan gambaran histopatologi sel endotel arteri coronaria pada tikus putih (Rattus Norvegicus) yang diberi diet Aterogenik. Program Studi Pendidikan Dokter Hewan. Program Kedokteran Hewan. Universitas Brawijaya. 1-12. Available from: http://pkh.ub.ac.id/wp-content/uploads/2012/10/0911310033-Ari Purnamasari P.pdf. Accessed on 9 may 2015.

Wihastuti TA, Sargowo D, Rohman MS. Efek ekstrak daun kelor (Moringaoleifera) dalam menghambat aktifasi NfkB, ekspresi TNFα dan ICAM-1 pada HUVECS yang dipapar LDL teroksidasi. Jurnal Kardiologi Indonesia 2007; 28: 181-8.

Wattel A, Kamel S, Mentaverri R, Lorget F, Prouillet C, Petit JP, Fardelone P, Brazier M. Potent inhibitory effect of naturally occuring flavonoids quercetin and kaempferol on in vitro osteoclastic bone resorption. Biochem Pharmacol 2003; 65: 35-42.

Woo JT, Nakagawa H, Notoya M, Yonezawa T, Udagawa N, Lee IS, Ohnishi M, Hagiwara H, Nagai K. Quercetin suppressses bone resorption by inhibiting the differentiation andivation of osteoclast. Biol Pharm Bull 2004; 27(4): 504-9.

De Risi V, Clementini M, Vittorini G, Mannocci A, De Sanctis M. Alveolar ridge preservation techniques, A systematic review and meta- analysis of histological and histomorphometrical data. Clin Oral Implants Res 2015; 26(1): 50-68.

Logeart-Avramoglou D, Anagnostou F, Bizios R, Petite H. Engineering bone chalanges and obstacles. J Cell Mol Med 2005; 9(1): 72-84.


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