Effects of sarang semut (Myrmecodia Pendens Merr. & Perry) extracts on Enterococcus faecalis sensitivity

Cut Soraya, Hendra Dian Adhyta Dharsono, Dudi Aripin, Mieke H Satari, Dikdik Kurnia, Danny Hilmanto

= http://dx.doi.org/10.20473/j.djmkg.v49.i4.p175-180
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Abstract


Background: Enterococcus faecalis (E. faecalis) is a gram positive oral pathogen that reported at the main agent infection of endodontic treatment. Its activities are influenced by the virulence factors facilitating the interaction process between agents with host cells. Like aggregation substance, cytolysin, extracellular superoxide, gelatinase, hyaluronidase, sex pheromones, and surface adhesions molecules. Plant extracts are reported as the material antibacterial as well as E. faecalis in pathogenesis of endodontic infections. Purpose: Purpose of this study was to analyse of sarang semut extracts (Myrmecodia Pendens Merr. & Perry) towards sensitivity of E. faecalis. Method: This research used the methanol extract of sarang semut, E. faecalis ATCC 29212, and fosfomycin also chlorhexidine as the positive controls. Whereas, Bradford protein method was measured the concentration of the surface protein of E. faecalis and active component of the sarang semut extract. Result: Generally, the sarang semut extract possessed low sensitivity toward E. faecalis (13 mm), but on the concentrations of 100 µg/ml and 75 µg/ml better than inhibition of other concentrations, round 10.6-11.6 (mm). Specifically, on 100 µg/ml has indicator the minimal bactericidal concentration (MBC) on E. faecalis. Whereas minimal inhibition concentration (MIC) on the concentration of 3,125 µg/ml. Conclusion: Based on MBC and MIC assay, the extract of sarang semut has potential effects to adherence growth of E. faecalis, mainly on the highest concentration 100 µg/ml also MIC on 3,125 µg/ ml.

Keywords


Enterococcus faecalis; extracts of sarang semut; sensitivity; minimum bactericidal concentration; minimum inhibitory concentration

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References


Stuart CH, Schwartz SA, Beeson TJ, Owatz CB. Enterococcus faecalis: its role in root canal treatment failure and current concepts in retreatment. J Endod 2006; 32(2): 93-8.

Wang Z, Shen Y, Haapasalo M. Effectiveness of endodontic disinfecting solutions against young and old Enterococcus faecalis biofilms in dentin canals. J Endod 2012; 38(10): 1376-79.

Saber S, El-Hady SA. Development of an intracanal mature Enterococcus faecalis biofilm and its susceptibility to some antimicrobial intracanal medications; an in vitro study. Eur J Dent 2012; 6(1): 43-50.

Narayanan LL, Vaishnavi C. Endodontic microbiology. J Conserv Dent 2010; 13(4): 233.

Sava IG, Heikens E, Huebner J. Pathogenesis and immunity in enterococcal infections. Clin Microbiol Infect 2010; 16(6): 533-40.

Christo J, Zilm P, Sullivan T, Cathro P. Efficacy of low concentrations of sodium hypochlorite and low‐powered Er, Cr: YSGG laser activated irrigation against an Enterococcus faecalis biofilm. Int Endod J 2016; 49(3): 279-86.

Miller WR, Munita JM, Arias CA. Mechanisms of antibiotic resistance in enterococci. Expert Rev Anti Infect Ther 2014; 12(10): 1221-36.

Evans M, Davies J, Sundqvist G, Figdor D. Mechanisms involved in the resistance of Enterococcus faecalis to calcium hydroxide. Int Endod J 2002; 35(3): 221-8.

Arias-Moliz MT, Ferrer-Luque CM, Espigares-García M, Baca P. Enterococcus faecalis biofilms eradication by root canal irrigants. J Endod 2009; 35(5): 711-4.

Naber KG, Thomas P, Fünfstück R. Fosfomycin trometamol in patients with renal insufficiency and in the elderly. The International Arabic Journal of Antimicrobial Agents 2012; 2(1): 1-10.

Du T, Ma J, Yang P, Xiong Z, Lu X, Cao Y. Evaluation of antibacterial effects by atmospheric pressure nonequilibrium plasmas against Enterococcus faecalis biofilms in vitro. J Endod 2012; 38(4): 545-9.

Klančnik A, Piskernik S, Jeršek B, Možina SS. Evaluation of diffusion and dilution methods to determine the antibacterial activity of plant extracts. J Microbiol Methods 2010; 81(2): 121-6.

Bakkali F, Averbeck S, Averbeck D, Idaomar M. Biological effects of essential oils–a review. Food and Chem Toxicol 2008; 46(2): 446-75.

Crisnaningtyas F. Pemanfaatan sarang semut (Myrmecodia pendens) asal Kalimantan Selatan sebagai antibakteri. Jurnal Riset Industri Hasil Hutan 2010;2(2):31-35.

Apriyanti E, Kurnia DSD, Dharsono HDA, Satari MH. Flavonoid dari Myrmecodia pendans dan aktivitas antibakteri terhadap Enterococcus faecalis. Unpad Library 2015; 1(1): 1-9.

Ross JE, Sader HS, Schoenfeld ZI, Paukner S, Jones RN. Disk diffusion and MIC quality control ranges for BC-3205 and BC-3781, two novel pleuromutilin antibiotics. Journal of Clinical Microbiology 2012; 50(10): 3361-4.

Maraki S, Samonis G, Rafailidis PI, Vouloumanou EK, Mavromanolakis E, Falagas ME. Susceptibility of urinary tract bacteria to fosfomycin. Antimicrob Agents Chemother 2009; 53(10): 4508-10.

Gani BA, Chismirina S, Hayati Z, Bachtiar BM, Wibawan IWT. The ability of IgY to recognize surface proteins of Streptococcus mutans. Dental Journal (Majalah Kedokteran Gigi) 2009; 42(4): 189-93.

Sasidharan S, Chen Y, Saravanan D, Sundram K, Latha LY. Extraction, isolation and characterization of bioactive compounds from plants’ extracts. Afr J Tradit Complement Altern Med 2011; 8(1): 1-10.

Wolfensberger A, Sax H, Weber R, Zbinden R, Kuster SP, Hombach M. Change of antibiotic susceptibility testing guidelines from CLSI to EUCAST: influence on cumulative hospital antibiograms. PloS One 2013; 8(11): e79130.

Jorgensen JH, Turnidge JD. Susceptibility test methods: dilution and disk diffusion methods. In: Manual of clinical microbiology. Eleventh edition. Washington DC: ASM Press; 2015. p. 1253-73.

Mulyani Y, Bachtiar E, Kurnia MU. Peranan senyawa metabolik sekunder tumbuhan mangrove terhadap infeksi bakteri aeromonas hydrophila pada ikan mas (Cyprinus carpio L). Jurnal Akuatika 2013; IV(1): 1-9.

Cathro P, McCarthy P, Hoffmann P, Zilm P. Isolation and identification of Enterococcus faecalis membrane proteins using membrane shaving, 1D SDS/PAGE, and mass spectrometry. FEBS Open Bio 2016; 6(6): 586-93.

Riadh H, Imen F, Abdelmajid Z, Sinda F. Detection and extraction of anti-listeral compounds from Calligonum comosum, a medical plant from arid regions of Tunisia. Afr J Tradit Complement Altern Med 2011; 8(3): 322–7.

Gibson GW, Kreuser SC, Riley JM, Rosebury-Smith WS, Courtney CL, Juneau PL, Hollembaek JM, Zhu T, Huband MD, Brammer DW, Brieland JK, Sulavik MC. Development of a mouse model of induced Staphylococcus aureus infective endocarditis. Comp Med 2007; 57(6):563-9.

Dougnon TV, Klotoé JR, Sègbo J, Atègbo JM, Edorh AP, Gbaguidi F, Hounkpatin AS, Dandjesso C, Fah L, Fanou B, Dramane K, Loko F. Evaluation of the phytochemical and hemostatic potential of Jatropha multifida sap. Afr J Pharmacy and Pharmacol 2012; 6(26): 1943-48.

Yesilada E, Gurbuz I. Evaluation of the antiulcerogenic activity profile of a flavonol diglucoside from Equisetum palustre L. J Ethnopharmacol 2010; 131(1): 17-21.

Pessione A, Lamberti C, Cocolin L, Campolongo S, Grunau A, Giubergia S, Eberl L, Riedel K, Pessione E. Different protein

expression profiles in cheese and clinical isolates of Enterococcus faecalis revealed by proteomic analysis. Proteomics 2012; 12(3): 431-47.

Bøhle LA, Færgestad EM, Kent EV, Steinmoen H, Nes IF, Eijsink VGH, Mathiesen G. Identification of proteins related to the stress response in Enterococcus faecalis V583 caused by bovine bile. Proteome Sci 2010; 8(1): 37.

Schneewind O, Missiakas DM. Protein secretion and surface display in Gram-positive bacteria. Phil Trans R Soc B 2012; 367(1592): 1123-39.

Lagunin A, Filimonov D, Poroikov V. Multi-targeted natural products evaluation based on biological activity prediction with PASS. Curr Pharm Des 2010; 16(15):1703-17.

Abouda Z, Zerdani I, Kalalou I, Faid M, Ahami M. The antibacterial activity of Moroccan bee bread and bee-pollen (fresh and dried) against pathogenic bacteria. Research Journal of Microbiology 2011; 6(4): 376-84.

Świątecka D, Narbad A, Ridgway KP, Kostyra H. The study on the impact of glycated pea proteins on human intestinal bacteria. Int J Food Microbiol 2011; 145(1): 267-72.

Aqil F, Khan MSA, Owais M, Ahmad I. Effect of certain bioactive plant extracts on clinical isolates of β‐lactamase producing methicillin resistant Staphylococcus aureus. J Basic Microbiol 2005; 45(2): 106-14.

Teplitski M, Robinson JB, Bauer WD. Plants secrete substances that mimic bacterial N-Acyl homoserine lactone signal activities and affect population density-dependent behaviors in associated bacteria. Mol Plant Microbe Interact 2000; 13(6): 637-48.

Bakri IM, Douglas CW. Inhibitory effect of garlic extract on oral bacteria. Arch Oral Biol 2005; 50(7): 645-51.

Khan R, Islam B, Akram M, Shakil S, Ahmad A, Ali SM, Siddiqui M, Khan AU. Antimicrobial activity of five herbal extracts against multi drug resistant (MDR) strains of bacteria and fungus of clinical origin. Molecules 2009; 14(2): 586-97.

Cushnie TT, Lamb AJ. Antimicrobial activity of flavonoids. Int J Antimicrob Agents 2005; 26(5): 343-56.


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