The efficacy of sarang semut extract (Myrmecodia pendens Merr & Perry) in inhibiting Porphyromonas gingivalis biofilm formation

Zulfan M. Alibasyah, Ambrosius Purba, Budi Setiabudiawan, Hendra Dian Adhita, Dikdik Kurnia, Mieke H. Satari

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Background: Porphyromonas gingivalis (P. gingivalis) is a pathogenic bacteria present in the oral cavity involved in the pathogenesis of chronic periodontitis and biofilm. This mass of microorganisms represents one of the virulent factors of P. gingivalis which plays an important role as an attachment initiator in host cells. Sarang semut is a natural material possessing the ability to inhibit the growth of P. gingivalis. Purpose: This study aims to analyze the effect of sarang semut extract on the formation of P. gingivalis biofilm. Methods: The study used methanol sarang semut extract and P. gingivalis ATCC 33277 and phosphomycin as a positive control. Treatment was initiated by means of culturing. Biofilm test and P. gingivalis biofilm formation observation were subsequently performed by means of a light microscope at a magnification of 400x. Results: The formation of P. gingivalis biofilms tended to increase at 3, 6, and 9 hours. Results of the violet crystal test showed that concentrations of 100% and 75% of the sarang semut extract successfully inhibited the formation of P. gingivalis biofilm according to the incubation time. Meanwhile, the sarang semut extracts at concentrations of 50%, 25%, 12.5%, and 6.125% resulted in weak inhibition of the formation of P. gingivalis biofilm. The biofilm mass profile observed by a microscope tended to decrease as an indicator of the effects of the sarang semut extract. Conclusion: Sarang semut extract can inhibit the formation of P. gingivalis biofilm, especially at concentrations of 100% and 75%. Nevertheless, phosphomycin has stronger antibiofilm of P. gingivalis effects than those of the sarang semut extract at all of the concentrations listed above.


Porphyromonas gingivalis; biofilm; Sarang Semut extract; phosphomycin

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Casanova L, Hughes FJ, Preshaw PM. Diabetes and periodontal disease: a two-way relationship. Br Dent J. 2014; 217(8): 433–7.

Bostanci N, Belibasakis GN. Porphyromonas gingivalis: an invasive and evasive opportunistic oral pathogen. FEMS Microbiol Lett. 2012; 333(1): 1–9.

Darveau RP. Periodontitis: a polymicrobial disruption of host homeostasis. Nat Rev Microbiol. 2010; 8(7): 481–90.

Marsh PD, Moter A, Devine DA. Dental plaque biofilms: communities, conflict and control. Periodontol 2000. 2011; 55(1): 16–35.

Amano A. Bacterial adhesins to host components in periodontitis. Periodontol 2000. 2010; 52(1): 12–37.

Becattini S, Taur Y, Pamer EG. Antibiotic-induced changes in the intestinal microbiota and disease. Trends Mol Med. 2016; 22(6): 458–78.

Costalonga M, Herzberg MC. The oral microbiome and the immunobiology of periodontal disease and caries. Immunol Lett. 2014; 162(2): 22–38.

Gülçin İ. Antioxidant activity of food constituents: an overview. Arch Toxicol. 2012; 86(3): 345–91.

Kariu T, Nakao R, Ikeda T, Nakashima K, Potempa J, Imamura T. Inhibition of gingipains and Porphyromonas gingivalis growth and biofilm formation by prenyl flavonoids. J Periodontal Res. 2017; 52(1): 89–96.

Soraya C, Dharsono HDA, Aripin D, Satari MH, Kurnia D, Hilmanto D. Effects of sarang semut (Myrmecodia Pendens Merr. & Perry) extracts on Enterococcus faecalis sensitivity. Dent J (Maj Ked Gigi). 2016; 49(4): 175–80.

CLSI. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; Approved Standard. 9th ed. Wayne PA, editor. Vol. 32, M07-A9. USA: Clinical and Laboratory Standards Institute; 2012. p. 1-5.

Aliyu MM, Musa AI, Kamal MJ, Mohammed MG. Phytochemical screening and anticonvulsant studies of ethyl acetate fraction of Globimetula braunii on laboratory animals. Asian Pac J Trop Biomed. 2014; 4(4): 285–9.

Brackman G, Cos P, Maes L, Nelis HJ, Coenye T. Quorum sensing inhibitors increase the susceptibility of bacterial biofilms to antibiotics in vitro and in vivo. Antimicrob Agents Chemother. 2011; 55(6): 2655–61.

Bridier A, Dubois-Brissonnet F, Boubetra A, Thomas V, Briandet R. The biofilm architecture of sixty opportunistic pathogens deciphered using a high throughput CLSM method. J Microbiol Methods. 2010; 82(1): 64–70.

Bachtiar EW, Bachtiar BM, Dewiyani S, Surono Akbar SM. Enterococcus faecalis with capsule polysaccharides type 2 and biofilm-forming capacity in Indonesians requiring endodontic treatment. J Investig Clin Dent. 2015; 6(3): 197–205.

Heuker M, Gomes A, van Dijl JM, van Dam GM, Friedrich AW, Sinha B, van Oosten M. Preclinical studies and prospective clinical applications for bacteria-targeted imaging: the future is bright. Clin Transl Imaging. 2016; 4(4): 253–64.

Ashida H, Ogawa M, Kim M, Mimuro H, Sasakawa C. Bacteria and host interactions in the gut epithelial barrier. Nat Chem Biol. 2011; 8(1): 36–45.

Sabrah AHA, Yassen GH, Gregory RL. Effectiveness of antibiotic medicaments against biofilm formation of Enterococcus faecalis and Porphyromonas gingivalis. J Endod. 2013; 39(11): 1385–9.

Kuboniwa M, Lamont RJ. Subgingival biofilm formation. Periodontol 2000. 2010; 52(1): 38–52.

Kreth J, Herzberg MC. Molecular Principles of Adhesion and Biofilm Formation. In: Chávez de Paz LE, Sedgley CM, Kishen A, editors. The Root Canal Biofilm. Berlin, Heidelberg: Springer; 2015. p. 23–53.

Schlafer S, Ibsen CJS, Birkedal H, Nyvad B. Calcium-Phosphate-Osteopontin Particles Reduce Biofilm Formation and pH Drops in in situ Grown Dental Biofilms. Caries Res. 2017; 51(1): 26–33.

Pöllänen M, Paino A, Ihalin R. Environmental stimuli shape biofilm formation and the virulence of periodontal pathogens. Int J Mol Sci. 2013; 14(8): 17221–37.

Park JH, Lee J-K, Um H-S, Chang B-S, Lee S-Y. A periodontitis-associated multispecies model of an oral biofilm. J Periodontal Implant Sci. 2014; 44(2): 79–84.

Shrout JD, Chopp DL, Just CL, Hentzer M, Givskov M, Parsek MR. The impact of quorum sensing and swarming motility on pseudomonas aeruginosa biofilm formation is nutritionally conditional. Mol Microbiol. 2006; 62(5): 1264–77.

Davey ME. Techniques for the growth of porphyromonas gingivalis biofilms. Periodontol 2000. 2006; 42(1): 27–35.

Van Acker H, Coenye T. The role of efflux and physiological adaptation in biofilm tolerance and resistance. J Biol Chem. 2016; 291(24): 12565–72.

Marsh PD. Dental plaque as a microbial biofilm. Caries Res. 2004; 38(3): 204–11.

Bao K, Belibasakis GN, Thurnheer T, Aduse-Opoku J, Curtis M a, Bostanci N. Role of Porphyromonas gingivalis gingipains in multi-species biofilm formation. BMC Microbiol. 2014; 14(1): 1–8.

Teles FR, Teles RP, Uzel NG, Song XQ, Torresyap G, Socransky SS, Haffajee AD. Early microbial succession in redeveloping dental biofilms in periodontal health and disease. J Periodontal Res. 2012; 47(1): 95–104.

Martin B, Tamanai-Shacoori Z, Bronsard J, Ginguené F, Meuric V, Mahé F, Bonnaure-Mallet M. A new mathematical model of bacterial interactions in two-species oral biofilms. Amar S, editor. PLoS One. 2017; 12(3): 1–24.

Coll RC, O’Neill LAJ. New insights into the regulation of signalling by toll-like receptors and nod-like receptors. J Innate Immun. 2010; 2(5): 406–21.

Wakabayashi H, Kondo I, Kobayashi T, Yamauchi K, Toida T, Iwatsuki K, Yoshie H. Periodontitis, periodontopathic bacteria and lactoferrin. BioMetals. 2010; 23(3): 419–24.

Olesen SH, Ingles DJ, Yang Y, Schönbrunn E. Differential antibacterial properties of the MurA inhibitors terreic acid and fosfomycin. J Basic Microbiol. 2014; 54(4): 322–6.

Furchtgott L, Wingreen NS, Huang KC. Mechanisms for maintaining cell shape in rod-shaped gram-negative bacteria. Mol Microbiol. 2011; 81(2): 340–53.

Jakubovics NS, Kolenbrander PE. The road to ruin: the formation of disease-associated oral biofilms. Oral Dis. 2010; 16(8): 729–39.

Chagnot C, Listrat A, Astruc T, Desvaux M. Bacterial adhesion to animal tissues: Protein determinants for recognition of extracellular matrix components. Cell Microbiol. 2012; 14(11): 1687–96.


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