Bactericidal Effects of Extract Basil Leaves in In-vitro Study of Pseudomonas aeruginosa
Downloads
Introduction: Pseudomonas aeruginosa is the most common bacterial cause of nosocomial infections. Bacteria become resistant to antibiotics by various mechanisms, including producing enzymes that can damage antibiotics, change intracellular targets from antibiotics, and efflux pumps. Basil (Ocimum sanctum L.) is a traditional plant that is usually used as ingredients that contain antibacterial compounds including flavonoids, tannins, alkaloids, and eugenol. The aim was to determine the effectiveness of extract basil leaves for inhibiting and killing the growth of Pseudomonas aeruginosa.
Methods: We administrated extract basil leaves with a concentration of 100%, 50%, and 25% in the plate contained bacterium Pseudomonas aeruginosa. The result showed that there was no inhibition of bacterial growth in broth dilution for 8 hours and 24 hours. We continued to culture bacteria for 24 hours.
Results: The analysis showed extract basil leaves has bactericidal effects in 8 hours and 24 hours incubation significantly (p < 0.05). However, in 24 hours more effective as a bactericidal in 100% of concentration significantly (p < 0.05).
Conclusion: From this result, eugenol (a phenol derivative found in the ethanol extract of basil leaves) has the effect of damaging cell membranes. Phenol bonding with bacterial cell walls can disrupt the permeability of transport cell membranes, thus the bacteria will be disrupted and die.
Nugraheni R and Winarni S. Infeksi Nosokomial di RSUD Setjonegoro Kabupaten Wonosobo. Media Kesehatan Masyarakat Indonesia. 2012; 11: 94-100.
Koomanachai P, Tiengrim S, Kiratisin P and Thamlikitkul V. Efficacy and Safety of Colistin (Colistimethate Sodium) For Therapy Of Infections Caused By Multidrug-Resistant Pseudomonas aeruginosa and Acinetobacter baumannii in Siriraj Hospital, Bangkok, Thailand. International Journal of Infectious Diseases. 2007; 11: 402-6.
Angelina M, Turnip M and Khotimah S. Uji Aktivitas Antibakteri Ekstrak Etanol Daun Kemangi (Ocimum sanctum L.) terhadap Pertumbuhan Bakteri Escherichia coli dan Staphylococcus aureus. 2015.
Hapsari IP. Uji Fitokimia dan Uji Aktivitas Antibakteri Minyak Astri Daun Kemangi (Ocimum basilicum L.) terhadap Pertumbuhan Propionibacterium acnes ATCC 11827 secara In Vitro. Yogyakarta: Universitas Sanata Dharma, 2018.
Cockerill F, Clinical and Laboratory Standards I. Performance Standards for Antimicrobial Susceptibility Testing : Twenty-Third Informational Supplement. Wayne, PA: Clinical and Laboratory Standards Institute, 2013.
Mishra P and Mishra S. Study of Antibacterial Activity of Ocimum sanctum Extract against Gram Positive and Gram Negative Bacteria. 2011.
Summary for Policymakers. In: Intergovernmental Panel on Climate C, (ed.). Climate Change 2013 – The Physical Science Basis: Working Group I Contribution to the Fifth Assessment Report of the Intergovernmental Panel on Climate Change. Cambridge: Cambridge University Press, 2014, p. 1-30.
Fauzia RS. Uji Aktivitas Antibakteri Minyak Atsiri Daun Kemangi (Ocimum basilicum L.) terhadap Staphylococcus aureus dan Escherichia coli. 2007.
Dev N, Das A, Hossain M and Rahman S. Chemical Compositions of Different Extracts of Ocimum basilicum Leaves. Journal of Scientific Research. 2011; 3: 197-.
Joshi S, Jasra WA, Ismail M, Shrestha RM, Yi SL and Wu N. Herders' Perceptions of and Responses to Climate Change in Northern Pakistan. Environmental Management. 2013; 52: 639-48.
1. The journal allows the author to hold the copyright of the article without restrictions.
2. The journal allows the author(s) to retain publishing rights without restrictions
3. The legal formal aspect of journal publication accessibility refers to Creative Commons Attribution Share-Alike (CC BY-SA).
