Pengaruh Ekstrak Etanol Lidah Buaya (Aloe Vera) Terhadap Glukosa Darah Tikus Hiperglikemia- Terinduksi Streptozotocin
Downloads
ABSTRAK
Diabetes Mellitus (DM) merupakan penyakit kronik dengan prevalensi nasional 6,9% pada tahun 2013. Hiperglikemia merupakan karakteristik dari penyakit DM. Pengobatan antidiabetes memiliki beberapa efek samping, seperti peningkatan berat badan, hipoglikemia, toksisitas hepar dan ginjal. Beberapa penelitian sebelumnya membuktikan bahwa ekstrak etanol lidah buaya dapat menurunkan kadar glukosa darah, namun mekanisme penurunan glukosa darah masih belum jelas. Penelitian ini merupakan penelitian eksperimental, menggunakan 35 ekor hewan coba, dibagi dalam 5 kelompok yaitu kelompok kontrol normal, kontrol hiperglikemia, kelompok perlakuan yang diberi dosis ekstrak etanol lidah buaya bertingkat, diadaptasikan selama 14 hari kemudian diinduksi dengan injeksi STZ. Kelompok perlakuan diberikan ekstrak etanol lidah buaya sesuai dengan dosis tiap kelompok selama 21 hari personde intragastrik. Pemeriksaan BSN dan GD2PP menggunakan glukometer terkalibrasi. Data dianalisa dengan Kruskal-Wallis, dilanjutkan dengan Mann-Whitney. Hasil uji komparasi meunjukkan bahwa terdapat perbedaan bermakna antar kelompok (p<0,05) pada variabel Δ BSN-2PP. Dosis 250mg/kgBB memiliki efek yang lebih baik terhadap penurunan kadar glukosa darah.
Kata kunci : Ekstrak etanol lidah buaya, kadar glukosa darah, streptozotocin, sel otot rangka.
DAFTAR PUSTAKA
Alinejad-Mofrad, S., Foadoddini, M., Saadatjoo, S. A., & Shayesteh, M., 2015. Improvement of glucose and lipid profile status with Aloe vera in pre- diabetic subjects: a randomized controlled-trial. Journal of Diabetes and Metabolic Disorders, 14(1), 22. https://doi.org/10.1186/s40200-015-0137-2.
Beidokhti, M. N., & Jäger, A. K., 2017. Review of Antidiabetic Fruits, Vegetables, Beverages, Oils and Spices Commonly Consumed in the Diet. Journal of Ethnopharmacology.https://doi.org/10.1016/j.jep.2017.02.031. Cowsert, L. M. (2010). Biological Activities of Acemannan.
Damasceno, D. C., Netto, A. O., Iessi, I. L., Gallego, F. Q., Corvino, S. B., Dallaqua, B., Rudge, M. V. C. (2014). Streptozotocin-induced diabetes models: Pathophysiological mechanisms and fetal outcomes. BioMed Research International, http://dx.doi.org/10.1155/2014/819065 Retrieved from https://search.proquest.com/docview/1547916578?accountid=31533.
DeFronzo R.A., 2004. Pathogenesis of Type 2 Diabetes Mellitus, The Medical Clinics of North America 88 (2004)787-835. DOI:10.1016/j.mcna.2004.04.013.
Hastono S.P. dan Sabri L., 2011. Statistik Kesehatan, PT. RajaGrafindo Persada, Jakarta.
Jung J.Y., Lim Y., Moon M.S., Kim J.Y., Kwon O., 2011. Onion Peel Extracts Ameliorate Hyperglycemia and Insulin Resistance in High Fat Diet/Streptozotocin-Induced Diabetic Rats, Nutrition & Metabolism, 8:18.
Joseph, B., & Raj, S. J., 2010. Pharmacognostic And Phytochemical Properties Of Aloe Vera Linn – An Overview, International Journal of Pharmaceutical Sciences Review and Research, 4(2), 106–110.
Karunakaran, U., Park, S. J., Jun, D. Y., Sim, T., Park, K. G., Kim, M. O., Lee, I.K., 2015. Non-receptor tyrosine kinase inhibitors enhances β-cell survival by suppressing the PKCδ signal transduction pathway in streptozotocin – induced β-cell apoptosis. Cellular Signaling, 27, 1066-1074. http://dx.doi.org/10.1016/j.cellsig.2015.01.018.
Kementrian Kesehatan Republik Indonesia. 2011. Penyakit Tidak Menular Penyebab Kematian Terbanyak di Indonesia, diunduh 7 November 2017, jam 11.26 WIB, <http://www.depkes.go.id/index.php?vw=2%25id=1637>.
Kementrian Kesehatan Republik Indonesia. 2014. Situasi dan Analisis Diabetes,diunduh 7 November 2017, jam 11.30 WIB, <https://doi.org/24427659>.
Kemp, R.W., 2000. Handling and Restraint. The Laboratory Rat. Elsevier, Ch.3,31-43.
Noor, A., Gunasekaran, S., & Vijayalakshmi, M. A. 2017. Improvement of Insulin Secretion and Pancreatic β-cell Function in Streptozotocin-induced Diabetic Rats Treated with Aloe vera Extract,Pharmacognosy Research.99–105. https://doi.org/10.4103/pr.pr.
Revathy, C., Arun, M. D., & Crispin, R. D., 2015. Molecular and CellularEndocrinology. https://doi.org/http://dx.doi.org/10.1016/j.mce.2015.09.004.
Reza, S., Tabatabaei, F., Ghaderi, S., & Bahrami-tapehebur, M. 2017.
Biomedicine & Pharmacotherapy Aloe vera gel improves behavioral deficits and oxidative status in streptozotocin-induced diabetic rats. Biomedicine & Pharmacotherapy, 96(September), 279–290. https://doi.org/10.1016/j.biopha.2017.09.146.
Rodríguez-González, V. M., Femenia, A., González-Laredo, R. F., Rocha- Guzmán, N. E., Gallegos-Infante, J. A., Candelas-Cadillo, M. G., Rosselló, C., 2011. Effects of pasteurization on bioactive polysaccharide acemannan and cell wall polymers from Aloe barbadensis Miller. Carbohydrate Polymers, 86(4), 1675–1683. https://doi.org/10.1016/j.carbpol.2011.06.084.
Sen, S., De, R. C., & Biplab, D., 2016. Diabetes Mellitus in 21st Century.
Singapore: Springer Nature. https://doi.org/10.1007/978-981-10-1542-7.
Soonthornpun S., Rattarasarn C., Leelawattana R., Setasuban W., 1999. Postprandial Plasma glucose: a good index of glycemic control in type 2 diabetic patients having near-normal fasting glucose levels. Diabetes Research and Clinical Practice, 46 (1999) 23-37, DOI:10.1016/s0168-8227(99)00061-3.
Tanaka, M., Misawa, E., Ito, Y., Habara, N., Nomaguchi, K., Yamada, M., Higuchi, R., 2006. Identification of Five Phytosterols from Aloe Vera Gel as Anti-diabetic Compounds. Biological & Pharmaceutical Bulletin, 29(7),1418–1422. https://doi.org/10.1248/bpb.29.1418.
Authors who publish with this journal agree to the following terms:
- The copyright of this journal belongs to the Editorial Board and Journal Manager with the author's knowledge, while the moral right of the publication belong to the author.
- The formal legal aspect of journal publication accessibility refers to the Creative Commons Attribution-Share Alike (CC BY-SA).
- Every publication (print/electronic) is open access for educational, research, and library purposes. In addition to the objectives mentioned above, the editorial board is not responsible for copyright infringement
