Daya Antibakteri Ekstrak Daun Belimbing Wuluh (Averrhoa bilimbi linn) terhadap Bakteri Enterococcus faecalis (Antibacterial Activity of Averrhoa bilimbi linn Leaf Extract against Enterococcus Faecalis)
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Background. The prevalence of Enterococcus faecalis bacterial infection caused the failure of root canal treatment between 24%-77%. This is due to various factors resistance and virulence of Enterococcus faecalis. This research to find alternative materials that have antibacterial properties and by utilizing natural ingredients that can later be used as a root canal irrigation. Antibacterial activity of the Averrhoa bilimbi linn leaf extract against Enterococcus faecalis bacteria can be determined by Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Purpose. The aim of this study was to determine the concentration of Averrhoa bilimbi linn leaf extract that has antibacterial activity against bacteria Enterococcus faecalis. Method. This research is a labolatory experimental with post test only control group design which use diluted Enterococcus faecalis ATCC 29212 according Mc. Farland standard 1,5 x 108 CFU/ml. With treatment Averrhoa bilimbi linn leaf extract on concentration 50%, 45%, 40%, 35%, 30%, and 25% given to each of 0,05 ml Enterococcus faecalis and using Brain Heart Infusion Broth (BHIB) as planting media. Result. At the concentration 30% of Averrhoa bilimbi linn leaf extract, showed that colony's growth less than 10%. At the concentration 35% was not revealed any bacterial growth. Conclusion. The Averrhoa bilimbi linn leaf extract has antibacterial effect on bacteria Enterococcus faecalis. The MIC was at 30% and MBC was at 35%.
Abidin, Trimurni. 2007. Inovasi Perawatan Konservasi Gigi Melalui Teknologi Tissue Engineering. Naskah Publikasi Universitas Sumatra Utara ; p.7-9.
Shahani, Reddy S. 2011. Comparison of Antimicrobial Substantivy of Root Canal Irrigants In Indtrument Root Canal up to 72h : An in vitro study. Journal of the Indian Society of Pedodontics & Preventive Denstistry; Vol. 29: p. 28-33
Meyyapan R, Sai Satya N, Kannan K, Shafie A, Vinoth Kumar, Deepa Arum. 2014. Effectiveness of Conventional and three differentt Rotary Retreatment techniques in canalsobturated with Gutta Percha : A Scanning Electron Microscopic Study. Departement of Conservative Dentistry and Endodontics. Annamalai Universuty. Tamilnadu ; vol : 26; p.259
Stuart CH, Schwartz SA, Beeson TJ, Owatz CB. 2006. Enterococcus faecalis: its role in root canal treatment failure and current concepts in retreatment. J Endod; vol.32(2): p. 93-7.
Marcos SE, Calo CR, Alexandre AZ, Jose FA, Brenda PF. 2013. Quantitive and Qualitative analysis of microorganisms in root-filled teeth with persistent infection : Monitoring of the endodontic retreatment. Departement of Restorative Dentistry, Endodontic Division. State University of Campinas. Brazil ; vol;7 ; p.302
Fisher K, Phillips C. 2009. The ecology, epidemiology and virulence of Enterococcus. University of Northampton, School of Health, Park Campus, Boughton Green Road, Northampton NN2 7AL, UK Microbiology; 155: p. 1749–57.
Kundabala M, Suchitra U. 2002. Enterococcus Faecalis: An Endodontic Pathogen. Journal of Endodontic; 3: p.11-3.
Hojo K, Nogaoka, Ohshima. 2009. Bacterial Interactions in Dental Biofilm Development. J Dent Ress 88 (11).p 987 - 990
Nisha, Amit Garg. 2010. Text Book of Endodontics. Jaypee Brothers. St Louis (USA). p 210.
Yunizar MF, S Larnani, N Archadian. 2014. Pengaruh Konsentrasi Minyak Atsiri Kayu Manis terhadap Daya Hambat Pertumbuhan E netrococcus faecalis. BIMKGI. Vol.2 ; p 1-9.
Depi P, Ratih M, Purwanto. 2012. Antibacterial Activity of Pare Leaf (Momordica charantia) Extract on Inhibition of Streptococcus viridans Growth. Jurusan Pendidikan Dokter Gigi, Fakultas Kedokteran Gigi, Universitas Jember (UNEJ). Artikel Ilmiah Hasil Penelitian Mahasiswa. p. 3.
Faharani, G B, 2009, Uji Aktifitas Antibakteri Daun Belimbing Wuluh Terhadap Bakteri Streptococcus Aureus dan Achercia Coli secara Bioautografi, FMIPA UI, Jakarta.p 23-25
Hayati E K, Jannah A, dan Mukhlisoh W. 2010. Pengaruh Ekstrak Tunggal dan Gabungan Daun Belimbing Wuluh (Averrhoa bilimbi Linn) Terhadap Efektivitas Antibakteri Secara In Vitro. Kimia. UIN Malang. Malang. p 12-14
Cushine TP, Andrew J Lamb. 2005. Review Antimicrobial activity of flavonoids. School of Pharmacy, The Robert Gordon University, Schoolhill, Aberdeen AB10 1FR, UK.Elsevier. vol. 26. p. 343 – 356.
Smullen J, Koutsou G A, Foster H A, Zumbe A, Storey DM. 2007. The antibacterial activity of Plant Extracts Containing Polypenols againts Streptococcus mutans. Caries Res. Vol. 41. p 342 – 349.
Guaijaverin ,Prabu GR, Gnanamani A, Sadulla S. Guaijaverin. 2006. A plant flavonoid as potential antiplaque agent against Streptococcus mutans. Journal of Applied Microbiology;101(2): p. 487-495.
Mailoa MN, Mahendradatta M, Laga A, Djide N. 2014. Antimicrobial Activities Of Tannins Extract From Guava Leaves (Psidium Guajava L) On Pathogens Microbial. International Journal Of Scientific & Technology Research;3(1): p. 236-241.
Juliantina F, Citra D A, Nirwani B, Nurmasitoh T, Bowo ET., 2009. Manfaat Sirih Merah (Piper crocatum) sebagai Agen Antibakterial terhadap Bakteri Gram Positif dan Gram Negatif. Jurnal Kedokteran dan Kesehatan Indonesia 1(1): p. 2-3.
Rijayanti, Rika. 2014. Uji Antibakteri Ekstrak Etanol Daun Mangga Bacang (Mangifera foetida l) terhadap Staphylococcus aureus secara In Vitro. Naskah Publikasi Universitas Tanjungpura ; p.7-9
Nanin DR. 2010. Daya Antibakteri Tumbuhan Majapahit (Crescentia Cujete L.) terhadap Bakteri Vibrio Alginolyticus. Jurusan Biologi, Fakultas Matematika Ilmu Pengetahuan Alam Institut Teknologi Sepuluh Nopember. p. 9.
Cushnie & Lamb. 2011. Recent Advances in Understanding the Antimicrobial Properties of Flavonoids. International Journal of Antimicrobial Agent; vol.38. p. 99-107.
Priggenies D, Ferawaty, A.S., Agus, S.,. 2012. Potensi Antibakteri Ekstrak Rumput Laut Terhadap Bakteri Penyakit Kulit Pseudomonas aeruginosa, Staphylococcus epidermidis, dan Micrococcus luteus. Jurnal of Marine Research. vol. 1(2): p. 152-160.
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