EKSPRESI TGF-B DAN MMP-1 PADA PERIODONTITIS APIKALIS KRONIS AKIBAT INDUKSI BAKTERI ENTEROCOCCUS FAECALIS TIKUS WISTAR
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Background. The main etiology of endodontic treatment failure is caused by bacteries that stay in the root canal. E.faecalis is a bactery that is found as an etiology of endodontic treatment failure. Cell wall of this bacteria is containing Lipoteichoic acid (LTA). LTA can penetrate into the periradicular tissue, act as endotoxin in host and cause periradicular inflammation and destruction. It occurs due to the capability of TGF-íŸ to enhance the proliferation collagen and MMP-1 to stop the collagen formation. The ability of enterococcus faecalis in enhancing inflamation process cause host can not reach the homeostasis phase and performing an even bigger tissue damage. Purpose. The aim of this study is to know about the expression of of TGF-íŸ and MMP-1 during the periapical tissue damage due to induction of E.faecalis. Method. This study used laboratory experimental with the post test only control group design. A total of 27 male rats were randomly divided into 3 main groups. Group A (negative control) : every tooth was'nt induced by anything. Group B ( positive control): every tooth was induced only by sterile BHIb and closed by GIC Fuji II as the final restoration. Group C (: every tooth was induced by 10 μl BHI-b E.faecalis ATCC212(106 CFU), and closed by GIC Fuji II as the final restoration. The animals were sacrificed after 21 days and prepared for histological examination of tissue damage, then we did the immunohistochemistry followed by calculation on the light microscope. Result. The analysis revealed that the expression of MMP-1 increased significantly in group C when E.faecalis was induced. When expression of TGF-íŸ decreaced significantly in group C rather than group B. Conclusion. From this study we know that the expression of TGF-íŸ and MMP-1 are make opposite pathway due to chronic apical periodontitis that induced by E.faecalis.
Nair. PNR. 2004. Pathogenesis of ApicalPeriodontitis and The Cause of Endodontic Failures, Criit Rev Oral Bio Med 15 : 348-51.
Bodromlu E, and Semis M. 2006.
Antibacterial Activity of A New
Endodontic Sealer Against Enterococcus faecalis. J. Can Dent Assoc.72(7):637a-c.
Stuart CH, Schawrtz SA, Beeson TJ and Owatz CB. 2006. Enterococcus faecalis :Its Role in Root Canal Treatment Failure and
Current Concepts in Re-Treatment. J.Endod. 32 (2) : 93-8.
Gemmell E, Carter C, Hart D, Drysdale K, and Seymour G. 2002. Antigen-presenting cells in human periodontal disease tissues. Oral Microbiology and Immunol, 17(6),
pp.388-93.
Lin SK, Kok SH, Kuo MY, Wang
TJ, Wang JT, Yeh FT, Hsiao M, Lan
WH, and Hong CY.2002.
Sequential expressions of MMP-1,
TIMP-1, IL-6, and COX-genes in
induced periapical lesions in rats.
J.Eur Oral Sci. ;110(3):246-53.
Fukada SY, Silva TA, Saconato IF, Garlet GP, Avila-Campos MJ, Silva JS, and Cunha FQ. 2008. iNOS-derived Nitric Oxide Modulates Infection-stimulated Bone Loss. J Dent Res. 87(12): 1155-9.
Abbot PV, 2004. Classification,
diagnosis, and clinical
manifestation of apical periodontitis. 2 nd edition, Endodontic topics. Copyright Blackwell Munksgaard. pp. 36-54.
Cohen S, and Hargreaves KM. 2011. Cohen'S Pathways of The pulp. 10th ed, St. Louis Missouri. Mosby Inc. pp. 529-58. Abbas AK, Lichtman AH and Pillai S.2010. Cellular and Molecular Immunology 6th ed Update edition. WB Saunders company USA. pp.281-2
Sabir, A. 2005. Respons Inflamasi Pada Pulpa Gigi Tikus Setelah Aplikasi Ekstrak Etanol Propolis (EEP). Majalah Kedokteran Gigi. (Dent.J.) 38 (2):77-83
Dammasckhe T, Stratmann U, Wolff P, Sagheri D dan Schafer E. 2010. Direct Pulp Capping with Mineral Trioxide Aggregate:
An Immunohistologic Comparison with Calcium Hydroxide in Rodents. Journal of Endodontics 36(5):814-9.
Baik JE, Han JY and Kum KY. 2008. Lipoteichoic Acid Partially Contributes to The Inflammatory Responses to Enterococcus faecalis. J.Endod. 34(8) : 975-82.
Pasare C and Medzhhirof R.2004. Toll-like Receptors : linking Innate and Adaptive Immunity. Microbes Infect 6 : 1382-7.
Thurlow LR, Chittezham T, Fleming S, dan Hancock L. 2009. Enterococcus faecalis Capsular Polysaccharide Serotypes C and D and their Contribution to Host Innate Immune Evasion. J Infection and Immunity 77(12): 5551-7.
Abbas AK, Lichtman AH and Pillai S.2010. Cellular and Molecular Immunology 6th ed Update edition. WB Saunders company USA.pp 281-2.
Sharon M. Wahl,1999.Wound Repair: Transforming growt Factor-β ( TGF-β ) in the Resolution and Repair of Inflammation : Basic Principples and Clinical Correlation.3rd ed.Philadhelphia: Gallin G I and Snyderman R, pp 865-7
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