Diversity Analysis of Genus Vibrio in Enterocytozoon Hepatopenaei (EHP) Infected Shrimp with Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC PCR) Method

Fenny Amelia, Beni Halalludin, Sidrotun Naim, Rega Permana

= http://dx.doi.org/10.20473/jafh.v9i3.18855
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Abstract


Udang merupakan salah satu komoditas unggulan nasional dan memiliki nilai ekonomi yang tinggi. Produksi udang budidaya di Indonesia tahun 2012-2017 mengalami kenaikan secara signifikan. Meski mengalami kenaikan, masih terdapat masalah yang harus diatasi, salah satunya adalah penyakit yang disebabkan oleh parasit Enterocytozoon Hepatopenaei (EHP). Infeksi EHP mengganggu sel tubulus hepatopankreas. Lesi yang disebabkan oleh EHP dapat menjadi tempat pertumbuhan koloni Vibrio sehingga meningkatkan kemungkinan munculnya penyakit lain. Penelitian ini bertujuan untuk mengetahui keanekaragaman Vibrio pada udang terinfeksi EHP yang dianalisis dengan Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC PCR). ERIC PCR dapat membedakan strain bakteri yang berkerabat dekat, prosedur kerja yang sederhana, cepat dan murah. Bakteri Vibrio diisolasi dari hepatopankreas, feses dan air udang terinfeksi EHP dan udang sehat (kontrol), dilanjutkan dengan ekstraksi DNA, ERIC PCR, elektroforesis dan konstruksi pohon filogenetik. Hasil penelitian menunjukkan bahwa jumlah bakteri Vibrio pada hepatopankreas, air dan feses udang terinfeksi EHP lebih tinggi dibandingkan dengan udang sehat. Udang terinfeksi EHP memiliki keanekaragaman Vibrio yang lebih rendah dari pada udang sehat. Hal ini menunjukkan adanya spesies Vibrio spesifik yang mendominasi pada udang terinfeksi EHP.

Keywords


Enterocytozoon Hepatopenaei; ERIC PCR; Vannamei Shrimp; Vibrio

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Aranguren, L.F., Han, J.E. dan Tang, K.F.J., 2017. Enterocytozoon hepatopenaei (EHP) is a risk factor for acute hepatopancreatic necrosis disease (AHPND) and septic hepatopancreatic necrosis (SHPN) in the Pacific white shrimp Penaeus vannamei. Aquaculture, 471, pp.37-42. https://doi.org/10. 1016/j.aquaculture.2016.12.038.

Asgarani, E., Ghashghaei, T., Soudi, M. R. dan Alimadadi, N., 2015. Enterobacterial repetitive intergenic consensus (ERIC) PCR based genetic diversity of Xanthomonas spp. and its relation to xanthan production. Iranian Journal of Microbiology, 7(1), pp. 38-44. https://www.ncbi.nlm.nih. gov/pmc/articles/PMC4670466/

Austin, B. dan Zhang, X.H., 2006. Vibrio harveyi: a significant pathogen of marine vertebrates and invertebrates. Letters in Applied Microbiology, 43(2), pp.119-124. https://doi.org/10.1111/j.1472-765X.2006.01989.x.

Balboa, S., Dieguez, A.L., Doce, A., Barja, J.L. dan Romalde, J.L., 2012. Evaluation of different culture media for the isolation and growth of the fastidious Vibrio tapetis, the causative agent of brown ring disease. Journal of invertebrate pathology, 111(1), pp.74-81. https://doi.org/10.1016/j.jip.2012.06.007.

Barton, L.L. dan McLean, R.J.C., 2019. Environmental Microbiology and Microbial Ecology. John Wiley & Sons, Inc. USA. p.445.

Bilung, L.M., Pui, C.F., Su’ut, L. dan Apun, K., 2018. Evaluation of BOX-PCR and ERIC-PCR as molecular typing tools for pathogenic Leptospira. Disease Markers, 2018, pp.1-10. https://doi.org/10.1155/2018/1351634.

Brown, S.P., Cornforth, D.M. dan Mideo, N., 2012. Evolution of virulence in opportunistic pathogens: generalism, plasticity, and control. Trends in microbiology, 20(7), pp.336-342. https://doi.org/10.10 16/j.tim.2012.04.005.

Clontz, L., 2009. Microbial Limit and Bioburden Test Validation Approaches and Global Requirements Second Edition. CRC Press. USA. p.326.

Csuros, M. dan Csuros, C., 1999. Microbial Examination of Water and Wastewater. CRC Press. USA. p.364.

Dong, X., Wang, H., Xie, G., Zou, P., Guo, C., Liang, Y. dan Huang, J., 2017. An isolate of Vibrio campbellii carrying the pirVP gen causes acute hepatopancreatic necrosis disease. Emerging Microbes & Infections, 6(1), pp.1-3. https://doi.org/10. 1038/emi.2016.131.

Feng, B., Liu, H., Wang, M., Sun, X., Pan, Y. dan Zhao, Y., 2017. Diversity analysis of acute hepatopancreatic necrosis disease-positive Vibrio parahaemolyticus strains. Aquaculture and Fisheries, 2(6), pp.278-285. https://doi.org/10.10 16/j.aaf.2017.10.001.

Gomez-Gil, B., Thompson, C., Matsumura, Y., Sawabe, T., Iida, T., Christen, R. dan Thompson, F., 2014. The Family Vibrionaceae, The prokaryotes. Springer, Berlin: Heidelberg. pp.659-747.

Holt, J.G., Krieg, N.R., Sneath, P.H.A., Staley, J.T. dan Williams, S.T., 1994. Bergey’s Manual of Determinate Bacteriology Ninth Edition. Lippincott Williams & Wilkins. USA. p.787.

Itsathitphaisarn, O., Thitamadee, S., Weerachatyanukul, W. dan Sritunyalucksana, K., 2017. Potential of RNAi applications to control viral diseases of farmed shrimp. Journal of invertebrate pathology, 147, pp.76-85. https:// doi.org/10.1016/j.jip.2016.11.006

Jaroenlak, P., Boakye, D.M., Vanichviriyakit, R., Williams, B.A.P., Sritunyalucksana, K. dan Itsathitphaisam, P., 2018. Identification, characterization and heparin binding capacity of a spora-wall, virulence protein from the shrimp microsporidian, Enterocytozoon hepatopenaei (EHP). Parasites & Vectors, 11 (177), pp.2-15. https://doi.org/ 10.1186/s13071-018-2758-z.

Lavilla-Pitogo, C.R. dan de la Peña, L.D., 1998. Bacterial diseases in tiger shrimp culture in the Philippines. SEAFDEC Asian Aquaculture, 20(5), pp.6-7, 10, 32-33. http:// repository.seafdec.org.ph/handle/10862/2883.

Maluping, R.P., Ravelo, C., Lavilla‐Pitogo, C.R., Krovacek, K. dan Romalde, J.L., 2005. Molecular typing of Vibrio parahaemolyticus strains isolated from the Philippines by PCR‐based methods. Journal of applied microbiology, 99(2), pp.383-391. https://doi.org/10.11 11/j.1365-2672.2005.02571.x.

Meacham, K.J., Zhang, L., Foxman, B., Bauer, R.J. dan Marrs, C.F., 2003. Evaluation of genotyping large numbers of Escherichia coli isolates by enterobacterial repetitive intergenic consensus-PCR. Journal of clinical microbiology, 41(11), pp.5224-5226. https://doi.org/10.1128/ jcm.41.11.5224-5226.2003.

Newman, S.G., 2015. Microsporidian impacts shrimp production-industry efforts address control, not eradication. Global Aquaculture Advocate, pp.33-35.

Parija, S.C., 2012. Textbook of Microbiology and Immunology 2nd Edition. Elsevier. Manesar. p.684.

Pascual, C., Ariadna, S., Edgar, Z., Gerard, C., Gaxiola, G., Roberto, B., Rolando, G., Enrique, H. dan Carlos, R., 2006. Biochemical, physiological, and immunological changes during starvation in juveniles of Litopenaeus vannamei. Aquaculture, 25(1), pp.416-429. https://doi.org/10.1016/j.aquaculture.2005.06.001.

Pusat Data Statistik, Kementerian Kelautan dan Perikanan (Sidatik), 2018. Dashboard Produksi Perikanan dan Kelautan Satu Data – Kementerian Kelautan dan Perikanan. Website https:// satudata.kkp.go.id/dashboard_produksi.

Pusparini, N., Waturangi, D.E., Usia, T. dan Nikastri, E., 2018. Genetic diversity of Escherichia coli isolated from ice cube production sites. BMC research notes, 11(1), pp.1-8. https://doi.org/10.1186/ s13104-018-3737-3.

Rajendran, K.V., Shivam, S., Praveena, P.E., Rajan, J.J.S., Kumar, T.S., Avunje, S., Jagadeesan, V., Babu, S.P., Pande, A., Krishnan, A.N. dan Alavandi, S.V., 2016. Emergence of Enterocytozoon hepatopenaei (EHP) in farmed Penaeus (Litopenaeus) vannamei in India. Aquaculture, 454, pp.272-280. https://doi.org/10.1016/j.aquaculture.2015.12.034.

Ramakrishnan, S. dan Sulochana, K.N., 2012. Manual of Medical Laboratory Techniques. Jaypee Brothers Medical Publishers. New Delhi. p.453.

Reantaso, M.B., Tran, L. dan Hue, D.T.T., 2013. What happens when hepatopancreas – shrimp’s main organ for food absorption, digestion and storage – becomes infected by a pathogen?. FAO Aquaculture Newsletter, 51, p.37-39.

Sechi, L.A., Zanetti, S., Dupré, I., Delogu, G., dan Fadda, G., 1998. Enterobacterial Repetitive Intergenic Consensus Sequences as molecular targets for typing of Mycobacterium tuberculosis strains. Journal Of Clinical Microbiology, 36(1), p.128-132. https://doi.org/ 10.1128/JCM.36.1.128-132.1998

Selvin, J., Ninawe, A.S., Meenatchi, R. dan Kiran, G.S., 2015. Control of pathogenic Vibrios in shrimp aquaculture using antiinfectives from marine natural products. Nutrición Acuícola: Investigación y Desarrollo, p.102-141. http://nutri cionacuicola.uanl.mx/index.php/acu/article/view/37.

Sharples, G.J. dan Lloyd, R.G., 1990. A novel repeated DNA sequence located in the intergenic regions of bacterial chromosomes. Nucleic acids research, 18(22), pp.6503-6508.https://doi.org/10.1093/nar/18.22.6503.

Tang, K.F., Aranguren, L.F., Piamsomboon, P., Han, J.E., Maskaykina, I.Y. dan Schmidt, M.M., 2017. Detection of the microsporidian Enterocytozoon hepatopenaei (EHP) and Taura syndrome virus in Penaeus vannamei cultured in Venezuela. Aquaculture, 480, pp.17-21. https: //doi.org/10.1016/j.aquaculture.2017.07.043.

Tourtip, S., Wongtripop, S., Stentiford, G.D., Bateman, K.S., Sriurairatana, S., Chavadej, J., Sritunyalucksana, K. dan Withyachumnarnkul, B., 2009. Enterocytozoon hepato-penaei sp. nov. (Microsporida: Enterocytozoonidae), a parasite of the black tiger shrimp Penaeus monodon (Decapoda: Penaeidae): Fine structure and phylogenetic relationships. Journal of invertebrate pathology, 102(1), pp.21-29. https://doi.org/10.1016 /j.jip.2009.06.004.

Waturangi, D.E., Joanito, I., Yogi, Y. dan Thomas, S., 2012. Use of REP- and ERIC-PCR to reveal genetic heterogeneity of Vibrio cholerae from edible ice in Jakarta, Indonesia. Gut Pathogens, 4(1), pp.1-9. https://doi.org/10.1186/ 1757-4749-4-2.

Wong, H.C. dan Lin, C.H., 2001. Evaluation of typing of Vibrio parahaemolyticus by three PCR methods using specific primers. Journal of Clinical Microbiology, 39(12), pp.4233-4240. https:// doi.org/10.1128/JCM.39.12.4233-4240.2001.

Zheng, Y., Yu, M., Liu, J., Qiao, Y., Wang, L., Li, Z., Zhang, X.H. dan Yu, M., 2017. Bacterial community associated with healthy and diseased Pacific white shrimp (Litopenaeus vannamei) larvae and rearing water across different growth stages. Frontiers in microbiology, 8(1362), pp.1-11. https://doi.org/10.3389/fmicb.2017.01362.

Zhou, J., Fang, W., Yang, X., Zhou, S., Hu, L., Li, X., Qi, X., Su, H., dan Xie, L., 2012. A nonluminescent and highly virulent Vibrio harveyi strains is associated with “Bacterial White Tail Disease” of Litopenaeus vannamei Shrimp. Plos One, 7(2), pp.1-6. https://doi.org/10.1371/ journal.pone.0029961.

Zulkifli, Y., Alitheen, N.B., Son, R., Raha, A.R., Samuel, L., Yeap, S.K. dan Nishibuchi, M., 2009. Random amplified polymorphic DNA-PCR and ERIC PCR analysis on Vibrio parahaemolyticus isolated from cockles in Padang, Indonesia. International Food Research Journal, 16(2), pp.141-150. http://www.ifrj. upm.edu.my/volume-16-2009.html


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