The Performance of Microalgae (Nannochloropsis sp., Tetraselmis sp. and Dunaliella sp.) on White Shrimp (Litopenaeus vannamei) Wastewater Cultivation Media
Microalgae have an important role in supporting the development of aquaculture because it can be used as natural feed. However, its culture requires an expensive cost because of the nutrient media. To reduce the cost, the media can be replaced by using wastewater from white shrimp (Litopenaeus vannamei) culture. This research was aimed to find out the performance of microalgae (Nannochloropsis sp., Tetraselmis sp. and Dunaliella sp.) cultured on white shrimp wastewater. The performance was measured by the growth, density, and ability to reduce nitrate and phosphate. The experimental design used in this study was a Completely Randomized Design with three treatments and three replications. The treatments were A (Nannochloropsis sp. cultured in white shrimp wastewater), B (Tetraselmis sp. cultured in white shrimp wastewater), and C (Dunaliella sp. cultured in white shrimp wastewater). The density population of Nannochloropsis sp., Tetraselmis sp. and Dunaliella sp. were tested by ANOVA. ANOVA was used to assess the density population of Nannochloropsis sp., Tetraselmis sp., and Dunaliella sp., which was then followed by Duncan's test. The results showed that wastewater from white shrimp aquaculture could be used as a medium culture for Nannochloropsis sp., Tetraselmis sp. and Dunaliella sp. It also maintain good water parameter quality in media. Nannochloropsis sp. was the microalgae that produced the highest density of 34.5 x 104 ind/mL when cultured on waste water from white shrimp culture. Nannochloropsis sp. may also reduce nitrate and phosphate content by up to 76 and 61.37 percent, respectively.
Adil, E.I., 2001. Penuntun praktikum fisiologi hewan. Jurusan Biologi FMIPA-UI, Depok, p. 57.
Agis, R. and Wahyu, S., 2015. Budidaya ikan kerapu macan. WWF Indonesia.
Amini, M., Khoei, Z.A., and Erfanifar, E., 2019. Nitrate (NO3−) and phosphate (PO4−) removal from aqueous solutions by microalgae Dunaliella salina. Biocatalysis and Agricultural Biotechnology, 19, 101097. https://doi.org/10.1016/j.bcab.2019.101097
Ansari, F.A., Singh, P., Guldhe, A., and Bux, F., 2017. Microalgal cultivation using aquaculture wastewater: integrated biomass generation and nutrient remediation. Algal research, 21, 169-177 pp. https://doi.org/10.1016/j.algal.2016.11.015
Balai Budidaya Laut., 2002. Budidaya fitoplankton dan zooplankton. Direktorat Jendral Perikanan. Departemen Kelautan dan Perikanan, 9, pp. 7-8.
Barkia, I., Saari, N., and Manning, S.R., 2019. Microalgae for high-value products towards human health and nutrition. Marine drugs, 17(5), p. 304. https://doi.org/10.3390/md17050304
Barsanti, L. and Gualtieri, P., 2006. Algae: Anatomy, biochemistry, and biotechnology. CRC Press. United States of America. 301 p.
Becker, B., Melkonian, M., and Kamerling, J. P., 1998. The cell wall (theca) of Tetraselmis striata (Chlorophyta): macromolecular composition and structural elements of the complex polysaccharides. Journal of phycology, 34(5), pp. 779-787. https://doi.org/10.1046/j.1529-8817.1998.340779.x
Ben-Amotz, A., 2009. The alga Dunaliella: Biodiversity, physiology, genomics and biotechnology. Science Publisher. America. pp. 357-493.
Boroh, R., Litaay, M., Umar, M.R., and Ambeng, A., 2019. Pertumbuhan Chlorella sp. pada beberapa kombinasi media kultur. BIOMA: Jurnal Biologi Makassar, 4(2), pp. 129-137. https://doi.org/10.20956/bioma.v4i2.6759
Chen, C. Y., Yeh, K. L., Aisyah, R., Lee, D. J., and Chang, J.S., 1994. Cultivation, photobioreactor design and harvesting of microalgae for biodiesel production: A critical review. Bioresource technology, 102(1), pp. 71-81. https://doi.org/10.1016/j.biortech.2010.06.159
Chen, Z., Ge, H., Chang, Z., Song, X., Zhao, F., and Li, J., 2018. Nitrogen budget in recirculating aquaculture and water exchange systems for culturing Litopenaeus vannamei. Journal of Ocean University of China, 17(4), pp. 905-912. https://doi.org/10.1007/s11802-018-3584-9
Ciotti, A.M., Lewis, M.R., and Cullen, J.J., 2002. Assessment of the relationships between dominant cell size in natural phytoplankton communities and the spectral shape of the absorption coefficient. Limnology and Oceanography, 47(2), pp. 404-417. https://doi.org/10.4319/lo.2002.47.2.0404
Daniel, N., Sivaramakrishnan, T., Saravanan, K., Shalini, B., Arunjyoti, B., Sankar, R., and Dann, R.S., 2016. A review on microalgae as potential fish feed ingredient. J. Andeman Sci. Assoc, 1, pp. 140-144. https://www.researchgate.net/publication/311312413_A_Review_on_Microalgae_as_Potential_Fish_Feed_Ingredient
Dewi N.R., Hadisoebroto R., Fachrul M.F., 2019. Removal of ammonia and phosphate parameters from greywater using Vetiveria zizanioides in subsurface-constructed wetland. Journal of Physics: Conference Series, 1402, 033012. https://doi.org/10.1088/1742-6596%2F1402%2F3%2F033012
Diharmi, A., 2001. Pengaruh pencahayaan terhadap kandungan pigmen bioaktif mikroalga Spirulina platensis strain local (INK). Doctoral dissertation, Institut Pertanian Bogor, 78 p.
Fachrullah, M.R., 2011. Laju Pertumbuhan mikroalga penghasil biofuel Jenis Chlorella sp. dan Nannochloropsis sp. yang dikultivasi menggunakan air limbah hasil penambangan timah di Pulau Bangka. Skripsi. IPB. Bogor, 102 p.
Felix, H.R., Chollet, R., and Harr, J., 1988. Use of the cell wall"less alga Dunaliella bioculata in herbicide screening tests. Annals of applied biology, 113(1), pp. 55-60. https://doi.org/10.1111/j.1744-7348.1988.tb03281.x
Han, P., Lu, Q., Fan, L., and Zhou, W., 2019. A review on the use of microalgae for sustainable aquaculture. Applied Sciences, 9(11), 2377. https://doi.org/10.3390/app9112377
Harianja, R.S.M., Anita, S., and Mubarak, M. 2018. Analisis beban pencemaran tambak udang di sekitar Sungai Kembung Kecamatan Bantan Bengkalis. Dinamika lingkungan Indonesia, 5(1), pp. 12-19. http://dx.doi.org/10.31258/dli.5.1.p.12-19
Indriana, N., Iba, W., Idris, M., and Ruslaini, R., 2020. Pengaruh kosentrasi pupuk organik cair lemna (Lemna minor) yang berbeda terhadap pertumbuhan mikroalga Chlorella vulgaris. Jurnal Media Akuatika, 5(1). http://dx.doi.org/10.33772/jma.v5i1.11754
Islam, M.S., 2005. Nitrogen and phosphorus budget in coastal and marine cage aquaculture and impacts of effluent loading on ecosystem: Review and analysis towards model development. Marine pollution bulletin, 50(1), pp. 48-61. https://doi.org/10.1016/j.marpolbul.2004.08.008
Manan, H., Moh, J.H.Z., Kasan, N.A., Suratman, S., and Ikhwanuddin, M., 2017. Identification of biofloc microscopic composition as the natural bioremediation in zero water exchange of Pacific white shrimp, Penaeus vannamei, culture in closed hatchery system. Applied Water Science, 7(5), pp. 2437-2446. https://doi.org/10.1007/s13201-016-0421-4
Musa, B., Raya, I., and Dali, S., 2013. Pengaruh penambahan ion Cu2+ terhadap laju pertumbuhan fitoplankton Chlorella vulgaris. Universitas Hasanuddin. Makassar, 9.
Nandiyanto, A.B.D., Haristiani, N., 2017 Design of simple water treatment system for cleaning dirty water in the rural area. IOP Conference Series: Materials Science and Engineering, 180, 012148. https://iopscience.iop.org/article/10.1088/1757-899X/180/1/012148/pdf
Rostini, I., 2007. Kultur fitoplankton (Chlorella sp. dan Tetraselmis chuii) pada skala laboratorium. Universitas Padjajaran. Jatinagor. 33 p.
Saiya, H.G. and Katoppo, D.R., 2015. Waste management of shrimp farms as starting point to develop integrated farming systems (case study: Kuwaru Coast, Bantul, Yogyakarta, Indonesia). Journal of Degraded and Mining Lands Management, 3(1), pp. 423-432. https://doi.org/10.15243/jdmlm.2015.031.423
Scholz, M.J., Weiss, T.L., Jinkerson, R.E., Jing, J., Roth, R., Goodenough, U., Posewitz, M.C., and Gerken, H.G., 2014. Ultrastructure and composition of the Nannochloropsis gaditana cell wall. Eukaryotic cell, 13(11), pp. 1450-1464. https://doi.org/10.1128/ec.00183-14
Sulistiowati, D., Tanjung, R.H., and Lantang, D., 2016. Keragaman dan kelimpahan plankton sebagai bioindikator kualitas lingkungan di perairan Pantai Jayapura. Jurnal Biologi Papua, 8(2).
Syah, R., Makmur, M. and Undu, M.C., 2014. Estimasi beban limbah nutrien pakan dan daya dukung kawasan pesisir untuk tambak udang vaname superintensif. Jurnal Riset Akuakultur, 9(3), pp. 439-448. http://dx.doi.org/10.15578/jra.9.3.2014.439-448
Tangguda, S., 2017. Pengaruh limbah cair tambak udang terhadap kepadatan sel dan laju pertumbuhan spesifik Chlorella sp. Seminar Nasional Riset Inovatif, 5, pp. 171-176. https://doi.org/10.14710/ijfst.14.2.96-99
Utomo, N.B.P., Winarti, Erlina, A., 2005. Pertumbuhan Spirulina platensis yang dikultur dengan pupuk inorganik (Urea, TSP, dan ZA) dan kotoran ayam. Jurnal Akuakultur Indonesia, 4(1), pp. 41-48. http://dx.doi.org/10.19027/jai.4.41-48
Yadav, G., Meena, D.K., Sahoo, A.K., Das, B. K., and Sen, R., 2020. Effective valorization of microalgal biomass for the production of nutritional fish-feed supplements. Journal of Cleaner Production, 243, 118697. https://doi.org/10.1016/j.jclepro.2019.118697
Yudha, A.P., 2008. Senyawa antibakteri dari mikroalga Dunaliella sp. pada umur panen yang berbeda. Skripsi. Bogor. Institut Pertanian Bogor.
Zheng, M., Schideman, L.C., Tomasso, G., Chen, W.T., Zhou, Y., Nair, K., Qian, W., Zhang, Y., Wang, K., 2017 Anaerobic digestion of wastewater generated from the hydrothermal liquefaction of Spirulina: Toxicity assessment and minimization. Energy conversion and management, 141, pp. 420-428. https://doi.org/10.1016/j.enconman.2016.10.034
Copyright (c) 2021 Muhammad Browijoyo Santanumurti, S.Pi., M.Sc
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
1. The copyright of this journal belongs to the Editorial Board, based on the author's consent, while the moral rights of the publication belong to the author(s).
2. The formal legal aspect of journal accessibility refers to the same Creative Common Attribution + Noncommercial + ShareAlike (CC BY-NC-SA), implying that publication can be used for non-commercial purposes in its original form.
3. Every publication (printed/electronic) is open access for educational, research and library purposes. In addition to the objectives stated above, the editorial board is not responsible for copyright infringement