The Effectiveness of Curcuma zedoaria Extract in Enhancing Non Spesific Immune Responses of Tiger Grouper (Epinephelus fuscoguttatus)
The present study was conducted to determine the effect of using turmeric extract (Curcuma zedoaria) on non specific immune of grouper (Epinephelus fuscoguttatus). During the study, fish were fed a diet containing 0, 0.5, 1.0, 2.5 g extract/kg feed twice a day for 14 days. Observations of non-specific immune parameters such as respiratory burst activity, chemiluminescent response, phagocytic activity, superoxidase dismutase (SOD) and lysozyme activity were sampled at 0, 1, 2, 4, 7 and 14 days. The results showed that fish fed C. zedoaria with 0.5 g/kg feed affected non-specific immune responses, and showed significant effect in the short term. On contrary, for long-term administration causes a decrease and inhibition of non-specific cellular defense activity. Thus, this study concluded that the administration of turmeric extract added to the feed can act as an immunostimulant and increase the non-specific immune response in grouper (E. fuscoguttatus).
Ardo L., Yin G., Xu P., Varadi L., Szigeti G., Jeney Z and Jeney G., 2008. Chinese herbs Astragalus membranaceus and Lonicera japonica and boron enhance the non specific immune response of Nile tilapia Oreochromis niloticus and resistance against Aeromonas hydrophila. Aquaculture, 275, pp. 26–33. https://doi.org/10.1016/j.aquaculture.2007.12.022
Bafna A.R. and Mishra S.H., 2005. Immunomodulatory activity of methanol extract of roots of Cissampelos pareira Linn. Ars Pharmaceutica, 46, pp. 253-262.
Bricknel, I. and Dalmo, R.A., 2005. The use of immunostimulants in fish larval aquaculture. Fish Shellfish Immunology, 19. pp. 457-472. https://doi.org/10.1016/j.fsi.2005.03.008
Cheng, A.C., Tu, C.W., Chen, Y.Y., Nan, F,H. and Chen, J.C., 2007. The immunostimulatory effect of sodium alginate and iota-carrageenan on orange-spotted grouper Epinephelus coioides and its resistance against Vibrio alginolyticus. Fish Shellfish Immunology, 22. pp. 197-205. https://doi.org/10.1016/j.fsi.2006.04.009
Chiu, S.T., Tsai, R.T., Hsu, J.P., Liu, C.H, and Cheng, W., 2008. Dietary sodium alginate administration to enhance the non specific immune responses and disease resistance of the juvenile grouper Epinephelus fuscoguttatus. Aquaculture, 277 pp. 66-72. https://doi.org/10.1016/j.aquaculture.2008.01.032
Citarasu, T., Babu, M.M., Sekar, R.J.R., and Marian, P.M., 2002. Developing Artemia enriched herbal diet for producing quality larvae in Penaeus monodon, Fabricius. Asian Fish Sci. 15. pp. 21-32. https://doi.org/10.33997/j.afs.2002.15.1.003
Dugenci, S.K., Arda N. and Candan A., 2003. Some medicinal plants as immunostimulant for fish. Journal of Ethnopharmacology, 88. pp. 99-106. https://doi.org/10.1016/s0378-8741(03)00182-x
Ellis, A.E., 1990. Lysozome Assay. In: Stolen, J.S., Anderson, B.S., Robertson, B.S (eds.), Techniques in Fish Immunology. Fair Heaven, NJ: SOS Publication.
Esteban, M.A., Mulero, V., Cuesta, A., Ortuño, J. and Meseguer, J., 2000. Effects of injecting chitin particles on the innate immune responses of gilthead seabream (Sparus aurata L). Fish Shellfish Immunol, 10. pp. 543-554. https://doi.org/10.1006/fsim.2000.0271
Fujiki, K. and Yano, T., 1997. Effect of sodium alginate on the non-specific defense system of Common carp (Cyprinus carpio L). Fish Shellfish Immunology, 7. pp. 417-427. https://doi.org/10.1006/fsim.1997.0095
Gopalakannan, A. and Arul, V., 2006. Immunomodulatory effects of dietary intake of chitin, chitosan and levamisole on the immune system of Cyprinus carpio and control of Aeromonas hydrophila infection in ponds. Aquaculture, 255. pp. 179-187. https://doi.org/10.1016/j.aquaculture.2006.01.012
Harikrishnan, R., Kim, J.S., Kim, M.C., Balasundaram, C., and Heo, M.S., 2011. Prunella vulgaris enhances the non-specific immune response and disease resistance of Paralichthys olivaceus against Uronema marinum. Aquaculture, 318. pp. 61-66. https://doi.org/10.1016/j.aquaculture.2011.05.020
Hou, J.P. and Jin, Y., 2005. The healing power of Chinese herbs and medical recipes. The Haworth Press. Inc. NY. pp. 565-567.
Kirubakaran, C.J.W., Subramani, P.A., Michael, R.D., 2016. Methanol extract of Nyctanthes arbortristis seeds enhances non-specific immune responses and protects Oreochromis mossambicus (Peters) against Aeromonas hydrophila infection. Research in Veterinary Science, 105. pp 243-248. https://doi.org/10.1016/j.rvsc.2016.02.013
Kuan, Y.C., Fuu, S., Lee, G.C., Tsai, M.W., Hung, C.L., and Nan, F.H., 2012. Administration of recombinant Reishi immunomodulatory protein (rLZ-8) diet enhances innate immune responses and elicits protection against nervous necrosis virus in grouper Epinephelus coioides. Fish Shellfish Immunology, 32. pp. 986-993. https://doi.org/10.1016/j.fsi.2012.02.008
Lee, C.H., Paek, N.S, Kim, D.S, and Kim, K.H., 2002. Effects of a Paecilomyces japonica supplemented diet on the chemiluminescent response of phagocytes and growth in juvenile olive flounder (Paralichthys olivaceus). Aquaculture, 208. pp. 51-57. https://doi.org/10.1016/S0044-8486(01)00799-2
Manfield, C., Trotter, C and Barber, A., 2005. Spices: Recipe to delight the senses. Periplus edition (HK) Ltd. Singapore. p. 252.
Metaxa, E., Deviller, G., Pagand, P., Alliaume, C., Casellas, C, and Blancheton, J.P., 2006. High rate algal pond treatment for water reuse in a marine fish recirculation: water purification and fish health. Aquaculture, 252. pp. 92-101. https://doi.org/10.1016/j.aquaculture.2005.11.053
Murray, A.L., Pascho, R.J., Alcorn, S.W., Fairgrievw, W.T., Shearer, K.D., and Roley, D.D., 2003. Effects of various feed supplements containing fish protein hydrolysate or fish processing by-products on the innate immune functions of juvenile coho salmon (Oncorhynchus kisutch). Aquaculture, 220. pp. 643-653. https://doi.org/10.1016/S0044-8486(02)00426-X
Nargis, A., Khatun, M., and Talukder, D., 2011. Use of medicinal plants in the remedy of fish diseases. Bangladesh Research Publication Journals, 5(3). pp. 192- 195.
Rottmann R.W., Francis-Floyed, R., and Durborow, R., 1992. The role of stress in fish disease. Southern Regional Aquaculture Center. Publication No. 474.
Sagdic, O. And Ozcan, M., 2003. Antibacterial activity of Turkish spice hydrosols. J. Food. Cont, 14. pp.141-143. https://doi.org/10.1016/S0956-7135(02)00057-9
Sahu S., Das B.K., Mishra B.K., Pradhan J. and Sarangi N., 2007. Effect of Allium sativum on the immunity and survival of Labeo rohita infected with Aeromonas hydrophila. Journal of Applied Ichthyology, 23. pp. 80-86. https://doi.org/10.1111/j.1439-0426.2006.00785.x
Sakai, M., 1999. Current research status of fish immunostimulants. Aquaculture, 172. pp. 63-92. https://doi.org/10.1016/S0044-8486(98)00436-0
Samad, A.P.A., Santoso, U., Lee, M.C., and Nan, F.H., 2014. Effects of dietary katuk (Sauropus androgynus L. Merr.) on growth, non-specific immune and diseases resistance against Vibrio alginolyticus infection in grouper Epinephelus coioides. Fish Shellfish Immunology, 36. pp. 582-589. https://doi.org/10.1016/j.fsi.2013.11.011
Sharp, G.J. and Secombes, C.J., 1993. The role of reactive oxygen species in the killing of the bacterial pathogen, Aeromonas salmonicida by rainbow trout macrophages. Fish Shellfish Immunology, 3. pp. 119-129. https://doi.org/10.1006/fsim.1993.1013
Taukhid, Suharni, I., and Supriyadi, H., 2007. Efektivitas ekstrak daun sambiloto (Andrographis paniculata) bagi pengendalian penyakit Koi Herpes Virus (KHV) pada ikan mas (Cyprinus carpio). Jurnal Riset Akuakultur, 2. pp. 411-418. http://dx.doi.org/10.15578/jra.2.3.2007.407-414
Verlhac, V., Gabaudan, J., Obach, A., Schuep, W., and Hole, R., 1996. Influence of dietary glucan and vitamin C on non-specific immune responses of rainbow trout (Oncorhynchus mykiss). Aquaculture, 143. pp. 123-133. https://doi.org/10.1016/0044-8486(95)01238-9
Yin, G., Ardo, L., Thompson, K.D., Adams, A., Jeney, Z., Jeney, G., 2009. Chinese herbs (Astragalus radix and Ganoderma lucidum) enhance immune response of carp, Cyprinus carpio, and protection against Aeromonas hydrophyla. Fish Shellfish Immunology, 26. pp.140-145. https://doi.org/10.1016/j.fsi.2008.08.015
Yin, G., Ardo, L., Jeney, Z., Xu, P. and Jeney, G., 2008. Chinese herbs (Lonicera japonica and Ganoderma lucidum) enhance non-specific immune response of tilapia, Oreochromis niloticus, and protection against Aeromonas hydrophila, pp. 269-282. In Bondad-Reantaso, M.G., Mohan, C.V., Crumlish, M. and Subasinghe, R.P. (eds.). Diseases in Asian Aquaculture VI. Fish Health Section, Asian Fisheries Society, Manila, Philippines.
Yeh, S.P., Chang, C.A., Chang, C.Y., Liu, C.H, and Cheng, W., 2008. Dietary sodium administration affects fingerling growth and resistance to Streptococcus sp and iridovirus, and juvenile non-specific immune responses of the orange-spotted grouper, Epinephelus coioides. Fish Shellfish Immunology, 25. pp. 19-27. https://doi.org/10.1016/j.fsi.2007.11.011
Copyright (c) 2021 Agus Putra AS
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
1. The copyright of this journal belongs to the Editorial Board, based on the author's consent, while the moral rights of the publication belong to the author(s).
2. The formal legal aspect of journal accessibility refers to the same Creative Common Attribution + Noncommercial + ShareAlike (CC BY-NC-SA), implying that publication can be used for non-commercial purposes in its original form.
3. Every publication (printed/electronic) is open access for educational, research and library purposes. In addition to the objectives stated above, the editorial board is not responsible for copyright infringement
.png)
