Antibacterial Activity of Red Galangal (A. purpurata) Extract on the Growth of E. tarda Bacteria
This study aimed to analyze the antibacterial activity of red galangal extract (Alpinia purpurata) against the growth of E. tarda. This aims of this study was to determine the antibacterial compounds and the antibacterial effectiveness of red galangal extract (A. purpurata) against the growth of E. tarda bacteria. Antibacterial activity test was carried out by MIC test and disc test. The MIC test used doses of 1 ppm, 10 ppm, 100 ppm, 500 ppm, and 1000 ppm with incubation for 24 hours. The disc test used doses of 100 ppm, 200 ppm, 300 ppm, 400 ppm, 500 ppm and a positive control of 5 ppm chloramphenicol and a negative control without treatment. Photochemical test of red galangal extract (A. purpurata) contains flavonoid compounds, alkaloids, tannins, triterpenoids, and saponins. The results of the disc test showed that the highest inhibitory diameter was 7.55 mm at a dose of 500 ppm and was bacteriostatic because it decreased its inhibitory zone after 48 hours of incubation. The highest inhibitory effectiveness was 56.56% at a dose of 500 ppm after 24 hours of incubation.
Alfiah, R.R., Khotimah, S. and Turnip, M., 2015. Efektivitas ekstrak metanol daun sembung rambat (Mikania micrantha Kunth) terhadap pertumbuhan jamur Candida albicans. Journal Protobiont, 4(1), pp. 52–57. http://dx.doi.org/10.26418/protobiont.v4i1.8735
Ariani, N. and Riski, A., 2018. Aktivitas ekstrak etanol kulit buah pisang kepok ,entah (Musa paradisiaca forma typica) terhadap prtumbuhan Candida albicans secara in vitro. Jurnal Pharmascience, 5(1), pp. 39–44. http://dx.doi.org/10.20527/jps.v5i1.5784
Azhar, F., Junaidi, M., Mukhlis, A. and Scabra, A.R., 2020. Penanggulangan penyakit mas (Motile Aeromonas Septicemia) pada ikan nila menggunakan ekstrak temulawak (Curcuma xanthorriza Roxb). Abdi Insani, 7(3), pp. 320–324. https://doi.org/10.29303/abdiinsani.v7i3.282
Devi, S., Ropiqa, M., Murti, Y.B. and Nugroho, A.K., 2020. Screening of extraction process and the estimation of total alkaloids in Carica papaya Linn. leaf. Traditional Medicine Journal, 25(2), pp. 90–95. http://dx.doi.org/10.22146/mot.52184
Fatimawali, Kepel, B.J. and Bodhi, W., 2020. Standarisasi parameter spesifik dan non-spesifik ekstrak rimpang lengkuas merah (Alpinia purpurata K. Schum) sebagai obat antibakteri. Jurnal E-Biomedik, 8(1), pp. 63–67. https://doi.org/10.35790/ebm.v8i1.28131
Hariati, S., Wahjuningrum, D., Yuhana, M., Tarman, K., Effendi, I. and Saputra, F., 2018. Aktivitas antibakteri ekstrak kapang laut Nodulisporium sp. KT29 terhadap Vibrio harveyi. Jurnal Pengolahan Hasil Perikanan Indonesia, 21(2), pp. 250-257. https://doi.org/10.17844/jphpi.v21i2.22855
Isramilda, I., Sahreni, S. and Saputra, A.I., 2020. Uji konsentrasi daya hambat rebusan daun srikaya (Annona squamosa L.) terhadap pertumbuhan Staphylococcus aureus. BEST Journal (Biology Education, Sains and Technology), 3(1), pp. 1–8. https://doi.org/10.30743/best.v3i1.2378
Kandou, L.A., Fatimawali, F. and Bodhi, W., 2016. Uji aktivitas antibakteri ekstrak etanol rimpang lengkuas merah (Alpinia purpurata (Vieill) K. Schum) terhadap bakteri Klebsiella pneumoniae. Pharmacon, 5(3), pp. 131–137. https://doi.org/10.35799/pha.5.2016.12947
Lasut, M.R.C., Fatimawali, F. and Antasionasti, I., 2019. Uji daya hambat nanopartikel ekstrak rimpang lengkuas merah (Alpinia purpurata K. Schum) terhadap pertumbuhan bakteri Klebsiella pneumoniae isolat urin pada penderita infeksi saluran kemih resisten antibiotik ciprofloxacin. Pharmacon, 8(4), pp. 11–21. https://doi.org/10.35799/pha.8.2019.29364
Maftuch, M., Setyawan, F.H. and Suprastyani, H., 2018. Uji daya hambat ekstrak Chaetoceros calcitrans terhadap bakteri Aeromonas salmonicida. JFMR-Journal of Fisheries and Marine Research, 2(1), pp. 39–46. https://doi.org/10.21776/ub.jfmr.2018.002.01.6
Mardhiyyah, K., Ryandini, Y.I. and Hermawan, Y., 2021. Uji aktivitas antioksidan dan skrining fitokimia perasan lengkuas merah dan lengkuas putih. Jurnal Jamu Indonesia, 6(1), pp. 23–31. https://doi.org/10.29244/jji.v6i1.174
Mogana, R., Adhikari, A., Tzar, M.N., Ramliza, R. and Wiart, C., 2020. Antibacterial activities of the extracts, fractions and isolated compounds from Canarium patentinervium miq. Against bacterial clinical isolates. BMC Complementary Medicine and Therapies, 20(1), pp. 1–11. https://doi.org/10.1186/s12906-020-2837-5
Nirwana, I., Rianti, D., Helal Soekartono, R., Listyorini, R.D. and Basuki, D.P., 2018. Antibacterial activity of fig leaf (Ficus carica Linn.) extract against Enterococcus faecalis and its cytotoxicity effects on fibroblast cells. Veterinary World, 11(3), pp. 342–347. https://doi.org/10.14202%2Fvetworld.2018.342-347
Nurhikmayani, R., Daryono, B.S. and Retnaningrum, E., 2019. Isolation and molecular identification of antimicrobial-producing lactic acid bacteria from chao, South Sulawesi (Indonesia) fermented fish product. Biodiversitas, 20(4), pp. 1063–1068. https://doi.org/10.13057/biodiv/d200418
Park, S.B., Aoki, T. and Jung, T.S., 2012. Pathogenesis of and strategies for preventing Edwardsiella tarda infection in fish. Veterinary Research, 43(1), pp. 1–11. https://doi.org/10.1186/1297-9716-43-67
Rialita, T., Rahayu, W.P., Nuraida, L. and Nurtama, B., 2015. Aktivitas antimikroba minyak esensial jahe merah (Zingiber officinale var. Rubrum) dan lengkuas merah (Alpinia purpurata K.Schum) terhadap bakteri patogen dan perusak pangan. Agritech, 35(1), pp. 43–52. https://doi.org/10.22146/agritech.9418
Riyadi, F.M., Prajitno, A., Fadjar, M., Syaifurrisal, A. and Fauziyyah, A.I., 2021. Potential of moringa (Moringa oleifera) leaf extract to inhibit the growth of pathogenic bacteria Edwardsiella tarda. Journal of Aquaculture and Fish Health, 10(3), pp. 321–330. https://doi.org/10.20473/jafh.v10i3.25057
Saidin, S., Jumat, M.A., Amin, N.A.A.M. and Al-Hammadi, A.S.S., 2021. Organic and inorganic antibacterial approaches in combating bacterial infection for biomedical application. Materials Science and Engineering C, 118(1), pp. 1–18. https://doi.org/10.1016/j.msec.2020.111382
Soelama, H.J.J., Kepel, B.J. and Siagian, K.V., 2015. Uji minimum inhibitory concentration (MIC) ekstrak rumput laut (Eucheuma cottonii) sebagai antibakteri terhadap Streptococcus mutans. E-GIGI, 3(2), pp. 374-379. https://doi.org/10.35790/eg.3.2.2015.9630
Warokka, K.E., Wuisan, J. and Juliatri., 2016. Uji konsentrasi hambat minimum (KHM) ekstrak daun binahong (Anredera cordifolia Steenis) sebagai antibakteri terhadap pertumbuhan Streptococcus mutans. E-GIGI, 4(2), pp. 155–163. https://doi.org/10.35790/eg.4.2.2016.13766
Yandri, O. and Setyani, W., 2021. Optimization of carbopol 940 and propylene glycol concentration on the characteristic and inhibitory effect of ethanol extract gel of papaya (Carica papaya L.) seeds against Staphylococcus aureus. Journal of Pharmaceutical Sciences and Community, 18(1), pp. 15–25. https://doi.org/10.24071/jpsc.002562
Yulvizar, C., Dewiyanti, I. and Defira, C.N., 2014. Seleksi bakteri berpotensi probiotik dari ikan mas (Cyprinus Carpio) indegenous jantho berdasarkan aktivitas antibakteri secara in vitro. Jurnal Teknologi Dan Industri Pertanian Indonesia, 6(2), pp. 44–49. http://dx.doi.org/10.17969/jtipi.v6i2.2066
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