Antibody Response in The Blood of Cantang Grouper Infected by Viral Nervous Necrosis with Recombinant Protein Chlorella Vulgaris Vaccination
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Cantang grouper (Epinephelus sp.) is one of the Export Oriented commodities which is a mainstay in increasing Indonesian mariculture production. The disease that causes many deaths in cantang grouper is Viral Nervous Necrosis. One of the most effective ways is by giving vaccines to fish. This study aims to develop a recombinant protein Chlorella vulgaris vaccine with the addition of an adjuvant to increase the immunity of cantang grouper infected with VNN. The method used was experimental with a Completely Randomized Design (CRD) consiting of seven treatments and three replications, namely (K+) cantang grouper infected with VNN, (T1) Healthy cantang grouper was given 33 µl of vaccine, (T2) Healthy cantang grouper was given vaccine of 66 µl, (T3) Healthy cantang grouper was given 112 µl of vaccine, (T4) Healthy cantang grouper was given 33 ul of vaccine and then tested against Viral Nervous Necrosis, (T5) Healthy cantang grouper was given 66 ul vaccine and then tested against Viral Nervous Necrosis, (T6) Healthy cantang grouper was given 112 ul vaccine and then tested against Viral Nervous Necrosis. The fish used were 8-10 cm in size and were reared for 56 days. The results showed that the Chlorella vulgaris protein recombinant vaccine with the addition of adjuvant gave a very significant effect on the immunity of cantang grouper infected with VNN with the best dose of 33 µl (T4) seen from the relative protection level (RPS) (75,6%), survival of cantang grouper (78%) after challenge test and a significant increase in antibody titer.
Arrokhman, S., Wijayanti, N., and Soegianto, A., 2017. Survival and osmoregulation of juvenile of hybrid grouper (Epinephelus fuscoguttatus í— Epinephelus lanceolatus) during acclimation in calcium-supplemented freshwater. Aquaculture International, 25(2). https://doi.org/10.1007/s10499-016-0069-y
Bahar, S.I., Harpeni, E., and Efendi, E. 2017. Respon imun spesifik larva ikan mas (Cyprinus carpio) melalui imunitas maternal yang diberi vaksin inaktif whole cell Aeromonas salmonicida. Biospecies, 10(1). https://doi.org/10.22437/biospecies.v10i1.3486
Bunlipatanon, P. and U-taynapun, K., 2017. Growth performance and disease resistance against Vibrio vulnificus infection of novel hybrid grouper (Epinephelus lanceolatus í— Epinephelus fuscoguttatus). Aquaculture Research, 48(4). https://doi.org/10.1111/are.13008
Dadar, M., Dhama, K., Vakharia, V.N., and Hoseinifar, S.H., 2017. Advances in aquaculture vaccines against fish pathogens: Global status and current trends. Reviews in Fisheries Science and Aquaculture. https://doi.org/10.1080/23308249.2016.1261277
Febri, S.P., Antoni, A., Rasuldi, R., Sinaga, A., Haser, T.F., Syahril, M., and Nazlia, S., 2020. Adaptasi waktu pencahayaan sebagai strategi peningkatan pertumbuhan ikan bawal air tawar (Colossoma macropomum). Acta Aquatica: Aquatic Sciences Journal, 7(2). https://doi.org/10.29103/aa.v7i2.2509
Firdaus, R.F., Lim, L-S., Kawamura, G., and Shapawi, R., 2016. Assessment on the acceptability of hybrid grouper, Epinephelus fuscoguttatus ♀ í— Epinephelus lanceolatus ♂ to soybean meal-based diets. AACL Bioflux, 9(2), pp. 284-290.
Fitrawati, F., Wibowo, M.H., Amanu, S., and Sutrisno, B., 2015. Isolasi dan identifikasi egg drop syndrome virus dengan uji hemaglutinasi dan hemaglutinasi inhibisi. Jurnal Sain Veteriner, 33(1), pp. 59-68.
Hardi, E.H., Nugroho, R.A., Isnansetyo, A., Agriandini, M., Kusuma, I.W., and Sidik, A.S., 2019. Simultaneous administration of Boesenbergia pandurata extract and vaccination to stimulate immune response in tilapia, Oreochromis niloticus. Pakistan Journal of Biological Sciences, 22(9), pp. 419-426. https://doi.org/10.3923/pjbs.2019.419.426
Inayati, N., Fihiruddin, F., and Getas, I.W., 2020. Efek imunostimulator kubis (Brassica oleracea Var. Capitata alba) terhadap titer imunoglobulin G (Ig G) pada kelinci yang diinduksi dengan sel darah merah domba. Jurnal Analis Medika Biosains (JAMBS), 7(2), pp. 130-139. https://doi.org/10.32807/jambs.v7i2.196
Jiang, N., Luo, L., Xing, W., Li, T., Yuan, D., Li, W., Ma, Z., Jin, L., and Ji, M., 2019. Generation and immunity effect evaluation of biotechnology-derived Aeromonas veronii ghost by PhiX174 gene E-mediated inactivation in koi (Cyprinus carprio koi). Fish and Shellfish Immunology, 86, pp. 327-334. https://doi.org/10.1016/j.fsi.2018.07.042
Jiet, C.W. and Musa, N., 2018. Teknik budidaya kerapu cantang (Ephinephelus fuscoguttatus-lanceolatus) pada Keramba jaring apung di Balai Budidaya Air Payau, Situbondo, Jawa Timur. Jurnal Ilmiah Perikanan dan Kelautan, 10(2), pp. 70-75. https://doi.org/10.20473/jipk.v10i2.10466
Khumaidi, A., Fadjar, M., Irnawati, F., Kilawati, Y., and Yanuhar, U., 2019. Mass mortality associated with viral nervous necrosis of hybrid grouper (Epinephelus sp.) cultured in city of grouper. in AIP Conference Proceedings, 2120, 070007. https://doi.org/10.1063/1.5115724
Lutfiyah, L. and Budi, D.S., 2019. Fluctuation of asymmetry of hybrid cantang grouper (Epinephelus fuscoguttatus x Epinephelus lanceolatus) originating from Situbondo and Bali. Journal of Aquaculture Science, 4(1), pp. 21-26. https://doi.org/10.31093/joas.v4i1.65
Mohd-Aris, A., Muhamad-Sofie, M.H.N., Zamri-Saad, M., Daud, H.M., and Ina-Salwany, M.Y., 2019. Live vaccines against bacterial fish diseases: A review. Veterinary World, 12(11), pp. 1806-1815. https://doi.org/10.14202/vetworld.2019.1806-1815.
Qomariyah, N., Suprapto, H., and Sudarno, S., 2017. Administration of vaccine formalin killed cell (FKC) Vibrio alginolyticus to increase survival rate (SR), antibody titre, and phagocytic leukocytes cantang grouper (Epinephelus sp.) against bacteria Vibrio alginolitycus. Jurnal Ilmiah Perikanan dan Kelautan, 9(1), pp. 15-24. https://doi.org/10.20473/jipk.v9i1.7625
Rahmandani, S. and Herlina, S., 2021. Fermentasi ekstrak daun rambusa (Passiflora foetida L) pada pakan untuk meningkatkan pertumbuhan benih ikan papuyu (Anabas testudineus). Jurnal Ilmu Hewani Tropika, 10(2), pp. 41–45. https://unkripjournal.com/index.php/JIHT/article/view/193/183
Sembiring, S.B.M., Wibawa, G.S., Mahardika, K., Widiastuti, Z., and Haryanti, H., 2018. Prevalensi infeksi viral nervous necrosis (VNN) dan iridovirus pada hatcheri dan budidaya ikan laut. Media Akuakultur, 13(2), pp. 83-90. http://dx.doi.org/10.15578/ma.13.2.2018.83-90
Song, M.F., Kang, Y.H., Zhang, D.X., Chen, L., Bi, J.F., Zhang, H.P., Zhang, L., Qian, A.D., and Shan, X.F., 2018. Immunogenicity of extracellular products from an inactivated vaccine against Aeromonas veronii TH0426 in koi, Cyprinus carpio. Fish and Shellfish Immunology, 81, pp. 176-181. https://doi.org/10.1016/j.fsi.2018.07.004
Sudirman, I., Syawal, H., and Lukistyowati, I., 2021. Profil eritrosit ikan mas (Cyprinus carpio L) yang diberi pakan mengandung vaksin Aeromonas hydrophila. Ilmu Perairan (Aquatic Science), 9(2), pp. 144-151. http://dx.doi.org/10.31258/jipas.9.2.p.144-151
Suhaili, S., Putra, W.K.A., and Yulianto, T., 2020. Efisiensi pakan dan pertumbuhan ikan kerapu cantang (Epinephelus fuscoguttatus x Epinephelus lanceolatus) dengan pemberian dosis enzim papain berbeda. Intek Akuakultur, 4(2). https://doi.org/10.31629/intek.v4i2.2397
Triastuti, J., Pursetyo, K.T., Monica, A., Lutfiyah, and Budi, D.S., 2018. Abnormalities of hybrid grouper (Epinephelus fuscoguttatus x Epinephelus lanceolatus) in Situbondo. in IOP Conference Series: Earth and Environmental Science, 137, 012035. https://doi.org/10.1088/1755-1315/137/1/012035
Wahyudi, Y.A., Yanuhar, U., and Maftuch, M., 2018. Expression of Hsp70 and β-actin genes as the immune response against viral nervous necrosis that infected Asian Seabass (Lates calcalifer). J. Exp. Life Sci., 8(3), pp. 139–146. https://doi.org/10.21776/ub.jels.2018.008.03.02
Wang, Y., Wang, X., Huang, J., and Li, J., 2016. Adjuvant effect of Quillaja saponaria saponin (QSS) on protective efficacy and IgM generation in turbot (Scophthalmus maximus) upon immersion vaccination. International Journal of Molecular Sciences, 17(3), p. 325. https://doi.org/10.3390/ijms17030325
Widjayanthi, L. and Widayanti, Y.A., 2020. Dampak penggunaan keramba jaring apung pada pembudidaya ikan kerapu berdasarkan perspektif social ekonomi. Jurnal KIRANA, 1(1), pp. 12-18. https://doi.org/10.19184/jkrn.v1i1.20310
Yamaguchi, T., Quillet, E., Boudinot, P., and Fischer., 2019. What could be the mechanisms of immunological memory in fish?. Fish and Shellfish Immunology, 85, pp. 3-8. https://doi.org/10.1016/j.fsi.2018.01.035
Yanti, A.H., Lestari, E., and Setyawati, T.R., 2019. Profil hematologi ikan gabus (Channa striata Bloch, 1793). Jurnal Protobiont, 8(2), 1-7. http://dx.doi.org/10.26418/protobiont.v6i3.22495
Yanuhar, U., 2021. Vaksin rekombinan protein p-PERCv untuk viral nervous necrosis. Indonesia. S00202109866
Yanuhar, U., 2020. Pengujian vaksin rekombinan protein peridinin P-PERCv Chlorella vulgaris untuk immunostimulan terhadap virus RNA pada komoditas ikan laut unggul. Indonesia, Granted: EC00202054834
Yanuhar, U., Musa, M., Arfiati, D., Caesar, N.R., and Junirahma, N.S., 2020. In-vivo test of Chlorella protein fragments as nucleotide vaccine candidates in grouper viral nervous necrosis (VNN) infection against haematological response. Proceedings of the 6th International Conference on Advanced Molecular Bioscience and Biomedical, 1, pp. 79-83. https://doi.org/10.5220/0009588100790083
Yanuhar, U. and Khumaidi, A., 2017. The application of pigment-protein fraction from Nannochloropsis oculata on β-actin response of Cromileptes altivelis infected with viral nervous necrosis. Jurnal Akuakultur Indonesia, 16(1). https://doi.org/10.19027/jai.16.1.22-32
Zhang, Z., Yu, A., Lan, J., Zhang, H., Hu, M., Cheng, J., Zhao, L., Lin, L., and Wei, S., 2017. GapA, a potential vaccine candidate antigen against Streptococcus agalactiae in Nile tilapia (Oreochromis niloticus). Fish and Shellfish Immunology, 63, pp. 255-260. https://doi.org/10.1016/j.fsi.2017.02.019
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