Analyzing the Ability of Various Chosen Medicinal Herbs to Cure Wounds in African Catfish (Clarias gariepinus, Burchell 1822)
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Phytomedicines are affordable, safe, and offer great potential for treating and controlling wounds. The effects of herbal supplements on Clarias gariepinus wound healing was investigated. Twenty-four fish (146.4±0.74g) randomised into four paired sub-groups: G1 and G2, G3 and G4, G5 and G6, G7, and G8 were fed formulated rations containing no herbal additive (0.0g/kg), 5.0g/kg of Allium sativum, 10.0g/kg of Chromolaena odorata and 10.0g/kg of Talinum triangulare as feed additive respectively at 5% body weight. On the 21st day, a sterile incision measuring 45.0 mm by 1.0 mm was made on the dorsolateral side of each fish. Subgroups G2, G4, G6, and G8 were intraperitoneally inoculated with 0.1 ml of pathogenic Pseudomonas aeruginosa that had been predetermined (1.4 x106 bacteria/ml), while G1, G3, G5, and G7 were not inoculated. Post-incision, macroscopic parameters (measurements), given as percentage healing rates on days 3, 6, 9, 12, and 15, were used to evaluate the wound closure. Data were analyzed using descriptive statistics and ANOVA at α0.05. On day 3, there were significant differences between the control and treatment groups in the healing pattern (P < 0.05). On day 15 post-incision, G7 had the best healing rate in both inoculated (86.7 percent) and uninoculated (100 percent) fish, while G1 had the lowest healing rate (0.0 percent) and maximum healing rate (64.4 percent), respectively. The findings indicated that Talinum triangulare at a concentration of 10.0 g/kg was the optimum feed additive for promoting wound healing in Clarias gariepinus.
Airaodion, A.I., Ogbuagu, E.O., Ekenjoku, J.A., Ogbuagu, U. and Airaodion, E.O., 2019. Haematopoietic potential of ethanolic leaf extract of Talinum triangulare in wistar rats. Asian Journal of Research in Biochemistry, 5(2), pp. 1-7. https://doi.org/10.9734/AJRB/2019/v5i230084
Aja, P.M., Okaka, A.N.C., Onu, P.N., Ibiam, U., Urako, A.J., 2010. Phytochemical composition of Talinum triangulare (Water Leaf) Leaves. Pakistan Journal of Nutrition, 9(6), pp. 527-530. https://doi.org/10.3923/pjn.2010.527.530
Aly, S.M. and Albutti, A., 2014. Antimicrobials use in aquaculture and their public health impact. Journal of Aquaculture Research & Development, 5(4), p. 1. https://doi.org/10.4172/2155-9546.1000247
Atibita, E.N.O., Fotsing, D., Mogho, N.M.T., Djieto-Lordon, C. and Fohouo, F.N.T., 2021. Influence of the Foraging Activity of the Anthophilous insects on Talinum triangulare (Waterleaf) fructification in Bafut (North West-Cameroon). Turkish Journal of Agriculture-Food Science and Technology, 9(12), pp. 2106-2112. https://doi.org/10.24925/turjaf.v9i12.2106-2112.4261
Bahramsoltani, R., Farzaei, M.H. and Rahimi, R., 2014. Medicinal plants and their natural components as future drugs for the treatment of burn wounds: an integrative review. Archives of dermatological research, 306, pp. 601-617. https://doi.org/10.1007/s00403-014-1474-6
Bazafkan, M.H., Ameneh, M., Reza, A.Z., Ashraf A.Z. and Mahmoud O.Z., 2014. Wound healing effect of an ointment made from a mixture of Brassica oleracea var, Punica granatum, and Plantago major L Extracts in Rats Jentashapir. Journal of Health Research, 5(4), e21877. https://doi.org./10.5812/jjhr.21877
Bene, C., Steel, E., Luadia, B.K. and Gordon, A., 2009. Fish as the "bank in the water” –Evidence from chronic-poor communities in Congo. Food policy, 34(1), pp. 108-118. https://doi.org/10.1016/j.foodpol.2008.07.001
Boudjeko, T., Megnekou, R., Woguia, A.L., Kegne, F.M., Ngomoyogoli, J.E.K., Tchapoum, C.D.N. and Koum, O., 2015. Antioxidant and immunomodulatory properties of polysaccharides from Allanblackia floribunda Oliv stem bark and Chromolaena odorata (L.) King and HE Robins leaves. BMC research notes, 8(1), pp. 1-9. https://doi.org/10.1186/s13104-015-1703-x
Calixto, J.B., 2000. Efficacy, safety, quality control, marketing and regulatory guidelines for herbal medicines (phytotherapeutic agents). Brazilian Journal of medical and Biological research, 33, pp. 179-189. https://doi.org/10.1590/s0100-879x2000000200004
Choudhary, S., Noor, M.U., Hussain, M.S., Mishra, M. and Tyagi, S., 2022. Pharmacological properties and phytoconstituents of garlic (Allium sativum L.): A review. Biological Sciences, 2(4), pp. 338-346. https://doi.org/10.55006/biolsciences.2022.2402
Corzo-Martinez, M., Corzo, N. and Villamiel, M., 2007. Biological properties of onions and garlic. Food Science and Technology, 18, pp. 609-625. https://doi.org/10.1016/j.tifs.2007.07.011
Dário, G.M., da Silva, G.G., Gonçalves, D.L., Silveira, P., Junior, A.T., Angioletto, E. and Bernardin, A.M., 2014. Evaluation of the healing activity of therapeutic clay in rat skin wounds. Materials Science and Engineering, C43, pp. 109-116. https://doi.org/10.1016/j.msec.2014.06.024
Dawid-Pac, R., 2013. Medicinal plants used in treatment of inflammatory skin diseases. Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii, 30(3), pp. 170-177. https://doi.org/10.5114/pdia.2013.35620
Dupont, E., Gomez, J. and Bilodeau, D., 2013., Beyond UV radiation: a skin under challenge. International journal of cosmetic science, 35(3), pp. 224-232. https://doi,org/10.1111/ics.12036
Eissa, A.E., Moustafa, M., El-Husseiny, I. N., Saeid, S., Saleh, O. and Borhan, T., 2009. Identification of some skeletal deformities in some freshwater teleost raised Egyptian aquaculture. Chemosphere, 77, pp. 419-425. https://doi.org/10.1016/j.chemosphere.2009.06.050
Eissa, A.E., Zaki, M.M. and Abdel Aziz, A., 2010. Flavobacterium columnare / Myxobolus tilapiae concurrent infection in the earthen pond reared nile tilapia (Oreochromis niloticus) during the early summer. IBC, 2(5), pp. 1-9. https://doi.org/10.4051/ibc.2010.2.2.000
Ezekwe, C.I., Uzomba, R. and Ugwu, P.C., 2013. The effect of methanol extract of Talinum Triangulare (water leaf on the hematology and some liver parameters of experimental rats. Global Journal of Biotechnology and Biochemistry, 8(2), pp. 51-60. https://doi.org/10.5829/idosi.gjbb.2013.8.2.1109
Gao, G., Xiao, K. and Chen, M., 2019. An intelligent IoT-based control and traceability system to forecast and maintain water quality in freshwater fish farms. Computers and Electronics in Agriculture, 166, 105013. https://doi.org/10.1016/j.compag.2019.105013
Harikrishnan, R., Balasundaram, C. and Heo, M.S., 2011. Impact of plant products on innate and adaptive immune system of cultured finfish and shellfish. Aquaculture, 317(1-4), pp. 1-15. https://doi.org/10.1016/j.aquaculture.2011.03.039
Kokane, D.D., More, R.Y., Kale, M.B. and Nehete, M.N., 2009. Evaluation of wound healing activity of root of Mimosa pudica. Journal of Ethnopharmacology, 124, pp. 311-315. https://doi.org/10.1016/j.jep.2009.04.038
Lieke, T., Meinelt, T., Hoseinifar, S.H., Pan, B., Straus, D.L. and Steinberg, C.E., 2020. Sustainable aquaculture requires environmental"friendly treatment strategies for fish diseases. Reviews in Aquaculture, 12(2), pp. 943-965. https://doi.org/10.1111/raq.12365
Martin, P. and Nunan, R., 2015. Cellular and molecular mechanisms of repair in acute and chronic wound healing. British Journal of Dermatology, 173(2), pp. 370-378. https://doi.org/10.1111/bjd.13954
Nagori, B.P. and Solanki, R., 2011. Role of medicinal plants in wound healing. Research Journal of Medicinal Plant, 5(4), pp. 392-405. https://doi.org/10.3923/rjmp.2011.392.405
Naumowicz, K., Pajdak, J., Terech-Majewska, E. anda Szarek, J. 2017. Intracohort cannibalism and methods for its mitigation in cultured freshwater fish. Reviews in Fish Biology and Fisheries, 27(1), pp. 193-208. https://doi.org/10.1007/s11160-017-9465-2
Noble, C., Cañon Jones, H.A., Damsgård, B., Flood, M.J., Midling, K.í˜., Roque, A., ... and Cottee, S.Y., 2012. Injuries and deformities in fish: their potential impacts upon aquacultural production and welfare. Fish physiology and biochemistry, 38(1), pp. 61-83. https://doi.org/10.1007/s10695-011-9557-1
Nya, E.J. and Austin, B., 2011. Development of immunity in rainbow trout (Oncorhynchus mykiss, Walbaum) to Aeromonas hydrophila after the dietary application of garlic. Fish and Shellfish Immunology, 30, pp. 845-850. https://doi.org/10.1016/j.fsi.2011.01.008
Ogbonnia, S.O., Mbaka, G.O., Igbokwe, N.H., Anyika, E.N., 2010. Antimicrobial evaluation, acute and subchronic toxicity studies of Leone Bitters, a Nigerian polyherbal formulation, in rodents. Agriculture and Biology Journal of North America, 1(3), pp. 366-376. https://doi.org/10.5251/abjna.2010.1.3.366.376
Ogbonnia, S., Adekunle, A.A., Bosa, M.K. and Enwuru, V.N., 2008. Evaluation of acute and subacute toxicity of Alstonia congensis Engler (Apocynaceae) bark and Xylopia aethiopica (Dunal) A. Rich (Annonaceae) fruits mixtures used in the treatment of diabetes. African Journal of Biotechnology, 7(6), pp. 701 -705. https://doi.org/10.5897/ajb08.144
Oo, K.S., Than, S.S. and Oo, T.H., 2019. A model HACCP plan for Fish seasoning powder production. American Journal of Food Science and Technology, 7(6), pp. 200-204. https://doi.org/10.12691/ajfst-5-6-7
Oyewole, O.I. and Akingbala, P.F., 2011. Phytochemical analysis and hypolipidemic poperties of Jatopha tanjorensis leaf extract. European Journal of Medicinal Plants, 1(4), pp. 180-185. https://doi.org/10.9734/EJMP/2011/497
Oz, A.T., 2010. Effects of harvest date and conditions of storage of Hayward kiwifruits on contents of L-ascorbic acid. Journal of Food, Agriculture and Environment, 8(2), pp. 132-134. https://doi.org/10.17660/actahortic.2010.876.8
Pandith, H., Thongpraditchote, S., Wongkrajang, Y. and Gritsanapan, W., 2012. In vivo and in vitro hemostatic activity of Chromolaena odorata leaf extract. Pharmaceutical biology, 50(9), pp. 1073-1077. https://doi.org/10.3109/13880209.2012.656849
Paul, T.S., Das, B.B., Ingale, S.P., Killedar, N. and Apte, K.G., 2018. Oral intake of polyphenols of Chromolaena odorata: A perspective in peptic ulcer, thrombocytopenia, and heparin-induced bleeding diathesis in rodent model. Pharmacognosy Research, 10(4), pp. 426-431. https://doi.org/10.4103/pr.pr_107_18
Paul, A. and Roychoudhury, A., 2021. Go green to protect plants: Repurposing the antimicrobial activity of biosynthesized silver nanoparticles to combat phytopathogens. Nanotechnology for Environmental Engineering, 6(1), p. 10. https://doi.org/10.1007/s41204-021-00103-6
Phan, T.T., See, P.O., Lee, S.T. and Chan, S.Y., 2001. Anti-oxidant effects of the extracts from the leaves of Chromolaena odorata on human dermal fibroblasts and epidermal keratinocytes against hydrogen peroxide and hypoxanthine–xanthine oxidase induced damage. Burns, 27(4), pp. 319-327. https://doi.org/10.1016/S0305-4179(00)00137-6
Rawling, M.D., Merrifield, D.L. and Davies, S.J., 2009. Preliminary assessment of dietary supplementation of Sangrovit on red tilapia (Oreochromis niloticus) growth performance and health. Aquaculture, 294, pp. 118-122. https://doi.org/10.1016/j.aquaculture.2009.05.005
Rex, J.R.S., Muthukumar, N.M.S.A. and Selvakumar, P.M., 2018. Phytochemicals as a potential source for anti-microbial, anti-oxidant and wound healing-a review. MOJ Biorg Org Chem, 2(2), pp. 61-70. https://doi.org/10.15406/mojboc.2018.02.0058
Saravanan, R., Senthilkumar, K., Dhachinamoorthi, D., Mahesh, P.U., Krishna, G.V. and Pavani, K.V., 2017. Evaluation of wound healing and antimicrobial activity of the Argemone mexicana linn (papavraceae). Research Journal of Pharmacy and Technology, 10(6), pp. 852-857. https://doi.org/10.5958/0974-360X.2017.00159.7
Sermakkani, M. and Thangapandian, V., 2012. GC-MS analysis of Cassia italica leaf methanol extract. Asian J Pharm Clin Res, 5(2), pp. 90-94. https://doi.org/10.9734/bpi/caprd/v3/9739d
Unuofin, J.O., 2020. Garbage in garbage out: The contribution of our industrial advancement to wastewater degeneration. Environmental Science and Pollution Research, 27(18), pp. 22319-22335. https://doi.org/10.1007/s11356-020-08944-5
Taiwo, O.B., Olajide, O.A., Soyannwo, O.O. and Makinde, J.M., 2000. Anti-inflammatory, antipyretic and antispasmodic properties of Chromolaena odorata. Pharmaceutical Biology, 38(5), pp. 367-370. https://doi.org/10.1076/phbi.38.5.367.5970
Tiamiyu, A., Olatoye, I. and Adedeji, O., 2019. Blood indices of African catfish (Clarias gariepinus) following dietary administration of Talinum triangulare. International Journal of Research–GRANTHAALAYAH, 7(4), pp. 185-198. https://doi.org/10.29121/granthaalayah.v7.i4.2019.888
Wang, W., Sun, J., Liu, C. and Xue, Z., 2017. Application of immunostimulants in aquaculture: current knowledge and future perspectives. Aquaculture Research, 48(1), pp. 1-23. https://doi.org/10.1111/are.13161
Zachariades, C., Day, M., Muniappan, R. and Reddy, G., 2009. Biological control of tropical weeds using Arthropods, eds: R. Muniappan, G.V.P. Reddy and A. Raman. Cambridge University Press, UK, pp. 130-160. https://doi.org/10.1017/CBO9780511576348.008
Zhang, W., Li, C. and Guo, M., 2021. Use of ecofriendly alternatives for the control of bacterial infection in aquaculture of sea cucumber Apostichopus japonicus. Aquaculture, 545, 737185. https://doi.org/10.1016/j.aquaculture.2021.737185
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