Effect of Extracellular Marennine produced by Haslea Ostrearia on the Blood Clams Tegillarca Granosa
Downloads
Marennine is a blue-green pigment produced by diatoms of the Haslea genus, one of which is Haslea ostrearia. This marennine pigment is water-soluble and confirmed to contain polyphenols and glycosides. There are two forms of marennine pigment: the intracellular form of marennine (IMn) and the extracellular form of marennine (EMn). Marennine pigments exhibit various biological activities such as antibacterial, antiviral, antioxidant, allelopathic, and inhibiting the growth of other diatoms. With this biological activity, marennine can be used in various fields, one of which is aquaculture.This research aims to determine the level of toxicity and analyze the effect of exposure to the extracellular marennine produced by H. ostrearia on the juvenile blood clam Tegillarca granosa. By using the toxicity test method, Blue water which is supernatant of H. ostrearia (BW) was tested on juvenile of commercially important bivalve species, the blood clams Tegillarca granosa, with three treatments, namely control (0 mg/L), treatment A (0.25 mg/L) and treatment B (0.5 mg/L of BW). The observation parameters of this study were LC50-72 h with one-way ANOVA analysis. The results showed that the BW produced by H. ostrearia increased the survival of juvenile blood clams T. granosa up to 27.7% in treatment B (0.5 mg/L) compared to control within 72 h. The one-way ANOVA analysis revealed that the control and treatment B (0.5 mg/L) were significantly different. This study shows that there is potential to develop the use of marennine in shellfish aquaculture.
Dey PM., and JB. Harborne, 1991. Methods in Plants in Biochemistry. Assay for Bioactivity 6. Academic Press, New York, Boston, Sydney Toyota, Toronto. p.196-198.
Gastineau, R., Hardivillier, Y., Leignel, V., Tekaya, N., Morancais, M., Fleurence, J., Davidovich, N., Jacquette, B., Gaudin, P., Hellio, C., Bourgougnon, N., and Mouget, JL., 2012. Greening Effect on Oysters and Biological Activities of The Blue Pigments Produced By The Diatom Haslea karadagensis (Naviculaceae). Journal of Aquaculture, p. 61 – 67. https://doi.org/10.1016/j.aquaculture.2012.09.016
Gastineau, R., Turcotte, F., Pouvreau J.B., and Morancais, M., 2014. Marennine, Promising Blue Pigments from a Widespread Haslea Diatom Species Complex. Journal of Marine Drugs, 12, p.3161 – 3189. https://doi.org/10.3390/md12063161
Gunawan, Adi and Roeswati., 2004. Tangkas Kimia. Kartika, Surabaya
Linnaeus, C., 1758. Systema Naturae per Regna trianaturae, Secundum Classes, Ordines, Genera, Species, Cum Characteribus, Differentiis, Synonymis, Locis. Editiodecima, reformata [10th revised edition], vol. 1: 824 pp.
Permatasari, I., Agung. MUK, Liviawaty, E., Astuty, S., Risyani, Y., Arsad, S., Mouget, JL, Prasetiya, FS., 2018. Antibacterial Activity of Haslea ostrearia Supernatant Adapted in Indonesia against Pathogenic Bacteria Relevant to Mariculture (In-Vitro studies). Omni-Aquatic Journal, 15(1): 30 – 38. http://dx.doi.org/10.20884/1.oa.2019.15.1.626
Pouvreau, J. B., Morançais, M., Fleurence, J., Pondaven, P., 2007. Method for the Quantification of the Blue-green Pigment "•Marennine"– Synthesized by the Marine Diatom Haslea ostrearia (Gaillon/Bory) Simonsen using HPLC gel-Filtration and Photodiode-array Detection. Journal of Applied Phycology, 19(3), p.263 – 270. https://doi.org/10.1007/s10811-006-9133-8
Pouvreau, J. B., Morançais, M., Taran, F., Rosa, P., Dufosse, L., Guedard, F., Pin, S., Fleurence, J., and Pondaven, P., 2008. Antioxidant and Free Radical Scavenging Properties of Marennine, a Blue-Green Polyphenolic Pigment from the Diatom Haslea ostrearia (Gaillon/Bory) Simonsen Responsible for the Natural Greening of Cultured Oysters. Journal of Agricultural and Food Chemistry, 56, p.6278 – 6286. https://doi.org/10.1021/jf073187n
Pouvreau, JB, Morançais, M., Fleury, F., Rosa, P., Thion, L., Cahingt, B., Zal, F., Fleurence. J., Pondaven, P., 2006. Preliminary Characterization of the Blue-green Pigment "Marennine” from the Marine Tychopelagic Diatom Haslea ostrearia (Gaillon/Bory) Simonsen. J. Appl. Phycol, 18, p.757 – 767.
https://link.springer.com/article/10.1007/s10811-006-9087-x
Prasetiya, F. S., Ramdhani, D. S., Mulyani, Y., Agung, M. U., Arsad, S., & Mouget, J. L., 2021. Antioxidant activities of culture supernatant Haslea ostrearia adapted in Indonesia. Aquaculture, Aquarium, Conservation & Legislation, 14(1), p.111-119.
Prasetiya, FS, Decottignies, P., Tremblay, R., Mouget, J. L., Sunarto, S., Iskandar, I., Dhahiyat, Y. & Cognie, B., 2020. Not only greening: The effects of marennine produced by Haslea ostrearia on physiological traits of three bivalve species. Aquaculture Reports, 18, p.100546.
https://doi.org/10.1016/j.aqrep.2020.100546
Prasetiya, FS, Safitri, I., Widowati, I., Cognie, B. Decottignis, P., Gastineau, R., Morançais, M., Windarto, E., Tremblay, R., and Mouget JL., 2016. Does Allelopathy Affect co-Culturing Haslea ostrearia with Other Microalgae Relevant to Aquaculture?. Journal of Applied Phycology, 28(4), p.2241 – 2254. https://doi.org/10.1007/s10811-015-0779-y
Prasetiya, FS, Sunarto, S., Bachtiar, E., Agung, MU, Nathanael, B., Pambudi, AC, Lestari A, D. Astuty, A. & Mouget, JL., 2020. Effect of the blue pigment produced by the tropical diatom Haslea nusantara on marine organisms from different trophic levels and its bioactivity. Aquaculture Reports, 17, p.100389. https://doi.org/10.1016/j.aqrep.2020.100389.
Prasetiya, FS., 2015. Greening Phenomenon in Bivalve by Marennine Produced from Haslea ostrearia and its Consequences on Bivalve's Integrated Response (Doctoral dissertation).
Prasetiya, F.S., Comeau, La., Gastineau, R., Decottignies, P., Cognie, B., Morançais, M., Turcotte, F., Mouget, J.-L., Tremblay, R., 2017a. Effect of marennine produced by the blue diatom Haslea ostrearia on behavioral, physiological and biochemical traits of juvenile Mytilus edulis and Crassostrea virginica. Aquaculture 467, 138–148. https://doi.org/10.1016/j.aquaculture.2016.08.029
Pratiwi, R., 2014. Benefits of Chitin and Chitosan for Human Life. Oceana, 39(1), 35 – 43.
Ramasamy, M. and Balasubramanian, U. 2012. Identification of bioactive compounds and antimicrobial activity of marine clam Anadara granosa (LINN.). International Journal of Science and Nature, 3(2): p.263 – 266.
Rohmani, I., 2014. Acute Toxicity Test of Tofu Factory Liquid Waste on Tilapia (Oreocromis niloticus) and Apu Wood Plants (Pistia stratiotes). Faculty of Civil Engineering and Planning, Sepuluh Nopember Institute of Technology, Surabaya.
Sahara, 2011. Characteristics of Blood Shells Anadara granosa. Department of Aquatic Products Technology, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University.
Sinardi, Soewondo prayatni, and Notodarmojo Suprihanto, 2013. Production, Characterization and Application of Chitosan from Green Shells (Mytulus verdis Linneaus) as a Water Purifying Coagulant. CONTEXT 7. p.24-26, Sebelas Maret University Campus (UNS).
Copyright (c) 2024 Eri Bachtiar, Ajeng Dinda Lestari, Sri Astuty, Sunarto Sunarto, Fiddy Semba Prasetiya
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
1. The copyright of this journal belongs to the Editorial Board, based on the author's consent, while the moral rights of the publication belong to the author(s).
2. The formal legal aspect of journal accessibility refers to the same Creative Common Attribution + Noncommercial + ShareAlike (CC BY-NC-SA), implying that publication can be used for non-commercial purposes in its original form.
3. Every publication (printed/electronic) is open access for educational, research and library purposes. In addition to the objectives stated above, the editorial board is not responsible for copyright infringement
.png)
