Effect of vitamin C supplementation on the survival rate and histopathological changes of gills and kidneys of tilapia (Oreochromis niloticus) infected by Aeromonas hydrophylla
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This study was conducted to determine the effect of vitamin C supplementation on the survival rate and histopathological changes of gills and kidneys of tilapia infected by Aeromonas hydrophila. Three doses of vitamin C were tested (150; 300; and 450 mg/kg) with two control groups. Tilapia with uniform size (average weight of 14 grams) as the criteria for inclusion were randomly distributed in five ponds with 15 tilapia fish per ponds. Tilapia was fed with hands until full for two weeks. Tilapia was then infected with A. hydrophila to find out the survival rate and histopathological changes of gills and kidneys at the end of the experimental period or 7 days after infection. The supplementation of 150 mg/kg vitamin C in feed increased the survival rate (%) in Tilapia by 86.67% or higher than other treatment and control groups. The damage to gills in terms of lamella separation was found in all treatment groups, as well as kidneys. The results found that the supplementation of 150 mg/kg vitamin C in feed increased survival rate but did not give effective protection on gills and kidneys.
Abdel Rahman, A.N., Khalil, A.A., Abdallah, H.M., ElHady, M., 2018. The effects of the dietary supplementation of Echinacea purpurea extract and/or vitamin C on the intestinal histomorphology, phagocytic activity, and gene expression of the Nile tilapia. Fish Shellfish Immunol. 82, 312–318. https://doi.org/10.1016/j.fsi.2018.08.024
Abdelhamed, H., Ibrahim, I., Baumgartner, W., Lawrence, M.L., Karsi, A., 2017. Characterization of Histopathological and Ultrastructural Changes in Channel Catfish Experimentally Infected with Virulent Aeromonas hydrophila. Front. Microbiol. 8. https://doi.org/10.3389/fmicb.2017.01519
Agarwal, S., Dinda, A., Sethi, S., 2013. Basics of kidney biopsy: A nephrologist"²s perspective. Indian J. Nephrol. 23, 243. https://doi.org/10.4103/0971-4065.114462
AlYahya, S.A., Ameen, F., Al-Niaeem, K.S., Al-Sa'adi, B.A., Hadi, S., Mostafa, A.A., 2018. Histopathological studies of experimental Aeromonas hydrophila infection in blue tilapia, Oreochromis aureus. Saudi J. Biol. Sci. 25, 182–185. https://doi.org/10.1016/j.sjbs.2017.10.019
APHA, 2005. Standard Methods for the Examination of Water and Wastewater, 21st ed.; APHA:Washington, DC, USA, 2005. Am. Water Work. Assoc. Public Work. Assoc. Environ. Fed. https://doi.org/10.2105/AJPH.51.6.940-a
Bakiyev, S., Smekenov, I., Zharkova, I., Kobegenova, S., Sergaliyev, N., Absatirov, G., Bissenbaev, A., 2022. Isolation, identification, and characterization of pathogenic Aeromonas hydrophila from critically endangered Acipenser baerii. Aquac. Reports 26, 101293. https://doi.org/10.1016/j.aqrep.2022.101293
Baroi, B., Rahman, M.H., Rohani, M.F., Hossain, 2019. Effect of dietary vitamin C on growth and survival of GIFT Tilapia. J. Agric. Rural Dev. 11, 37–42.
Carballeira Braña, C.B., Cerbule, K., Senff, P., Stolz, I.K., 2021. Towards Environmental Sustainability in Marine Finfish Aquaculture. Front. Mar. Sci. 8. https://doi.org/10.3389/fmars.2021.666662
Chen, H.-Y., Chang, C.-F., 1994. Quantification of Vitamin C Requirements for Juvenile Shrimp (Penaeus monodon) Using Polyphosphorylated L-Ascorbic Acid. J. Nutr. 124, 2033–2038. https://doi.org/10.1093/jn/124.10.2033
Chen, N., Jiang, J., Gao, X., Li, X., Zhang, Y., Liu, X., Yang, H., Bing, X., Zhang, X., 2018. Histopathological analysis and the immune related gene expression profiles of mandarin fish (Siniperca chuatsi) infected with Aeromonas hydrophila. Fish Shellfish Immunol. 83, 410–415. https://doi.org/10.1016/j.fsi.2018.09.023
Dawood, M.A.O., Koshio, S., 2018. Vitamin C supplementation to optimize growth, health and stress resistance in aquatic animals. Rev. Aquac. 10, 334–350. https://doi.org/10.1111/raq.12163
Elsheshtawy, A., Yehia, N., Elkemary, M., Soliman, H., 2019. Investigation of nile tilapia summer mortality in Kafr El-Sheikh Governorate, Egypt. Genet. Aquat. Org. 3. https://doi.org/10.4194/2459-1831-v3_1_03
Ferri, G., Lauteri, C., Vergara, A., 2022. Antibiotic Resistance in the Finfish Aquaculture Industry: A Review. Antibiotics 11, 1574. https://doi.org/10.3390/antibiotics11111574
Fracalossi, D.M., Allen, M.E., Yuyama, L.K., Oftedal, O.T., 2001. Ascorbic acid biosynthesis in Amazonian fishes. Aquaculture 192, 321–332. https://doi.org/10.1016/S0044-8486(00)00455-5
Garcia, F., Pilarski, F., Onaka, E.M., de Moraes, F.R., Martins, M.L., 2007. Hematology of Piaractus mesopotamicus fed diets supplemented with vitamins C and E, challenged by Aeromonas hydrophila. Aquaculture 271, 39–46. https://doi.org/10.1016/j.aquaculture.2007.06.021
Guimarí£es, I.G., Lim, C., Yildirim-Aksoy, M., Li, M.H., Klesius, P.H., 2014. Effects of dietary levels of vitamin A on growth, hematology, immune response and resistance of Nile tilapia (Oreochromis niloticus) to Streptococcus iniae. Anim. Feed Sci. Technol. 188, 126–136. https://doi.org/10.1016/j.anifeedsci.2013.12.003
Henriksson, P.J.G., Troell, M., Banks, L.K., Belton, B., Beveridge, M.C.M., Klinger, D.H., Pelletier, N., Phillips, M.J., Tran, N., 2021. Interventions for improving the productivity and environmental performance of global aquaculture for future food security. One Earth 4, 1220–1232. https://doi.org/10.1016/j.oneear.2021.08.009
Ibrahim, R.E., Ahmed, S.A.A., Amer, S.A., Al-Gabri, N.A., Ahmed, A.I., Abdel-Warith, A.-W.A., Younis, E.-S.M.I., Metwally, A.E., 2020. Influence of vitamin C feed supplementation on the growth, antioxidant activity, immune status, tissue histomorphology, and disease resistance in Nile tilapia, Oreochromis niloticus. Aquac. Reports 18, 100545. https://doi.org/10.1016/j.aqrep.2020.100545
Igbinosa, I.H., Igumbor, E.U., Aghdasi, F., Tom, M., Okoh, A.I., 2012. Emerging Aeromonas Species Infections and Their Significance in Public Health. Sci. World J. 2012, 1–13. https://doi.org/10.1100/2012/625023
Kamaruddin, A., Nurhudah, M., Rukmono, D., Wiradana, A., 2021. Potential of probiotics Bacillus subtilis to reduce ammonia levels, Vibrio sp abundance, and increased production performance of Seaworm (Nereis sp) under laboratory scale. Iraqi J. Vet. Sci. 35, 757–763. https://doi.org/10.33899/ijvs.2021.128408.1572
Karakaya, S., Yilmaz-Oral, D., Kilic, C.S., Gur, S., 2019. Umbelliferone isolated from Zosima absinthifolia roots partially restored erectile dysfunction in streptozotocin-induced diabetic rats. Med. Chem. Res. 28, 1161–1167. https://doi.org/10.1007/s00044-019-02359-9
Khalil, S.R., Reda, R.M., Awad, A., 2017. Efficacy of Spirulina platensis diet supplements on disease resistance and immune-related gene expression in Cyprinus carpio L. exposed to herbicide atrazine. Fish Shellfish Immunol. 67, 119–128. https://doi.org/10.1016/j.fsi.2017.05.065
Li, P., Feng, X., Chen, B., Wang, X., Liang, Z., Wang, L., 2022. The Detection of Foodborne Pathogenic Bacteria in Seafood Using a Multiplex Polymerase Chain Reaction System. Foods 11, 3909. https://doi.org/10.3390/foods11233909
Liu, X., Sun, W., Zhang, Y., Zhou, Y., Xu, J., Gao, X., Zhang, S., Zhang, X., 2020. Impact of Aeromonas hydrophila and infectious spleen and kidney necrosis virus infections on susceptibility and host immune response in Chinese perch (Siniperca chuatsi). Fish Shellfish Immunol. 105, 117–125. https://doi.org/10.1016/j.fsi.2020.07.012
Lu, T.-H., Chen, C.-Y., Liao, C.-M., 2021. Aeromonas hydrophila as an environmental indicator to detect TiLV-infected tilapia under coinfection threat. Environ. Sustain. Indic. 11, 100135. https://doi.org/10.1016/j.indic.2021.100135
Manyi-Loh, C., Mamphweli, S., Meyer, E., Okoh, A., 2018. Antibiotic Use in Agriculture and Its Consequential Resistance in Environmental Sources: Potential Public Health Implications. Molecules 23, 795. https://doi.org/10.3390/molecules23040795
Miao, L., Charles, O., Lin, Y., Gong, Y., Zhu, W., Wang, L., Fu, J., Zhang, Z., Dong, Z., 2020. Interactive effects of mulberry leaf meal and bamboo charcoal additive on growth performance, anti-oxidant capacity, and disease resistance of genetically improved farmed tilapia (GIFT) juvenile (Oreochromis niloticus). Aquac. Reports 18, 100483. https://doi.org/10.1016/j.aqrep.2020.100483
Mohanty, S., Ali, S.M., Singh, P.K., 2022. Necrotizing fasciitis and gas gangrene due to Aeromonas hydrophila in an immunocompetent host: A rare entity. IDCases 28, e01508. https://doi.org/10.1016/j.idcr.2022.e01508
Naylor, R.L., Hardy, R.W., Buschmann, A.H., Bush, S.R., Cao, L., Klinger, D.H., Little, D.C., Lubchenco, J., Shumway, S.E., Troell, M., 2021. A 20-year retrospective review of global aquaculture. Nature 591, 551–563. https://doi.org/10.1038/s41586-021-03308-6
Nikinmaa, M., 2014. Effects on Organisms, in: An Introduction to Aquatic Toxicology. Elsevier, pp. 111–146. https://doi.org/10.1016/B978-0-12-411574-3.00011-6
Ning, L., Zhang, H., Chen, X., Zhen, J., Chen, S., Guang, J., Xu, C., Li, Y., 2023. A comparative study on the tolerance of tilapia (Oreochromis niloticus) to high carbohydrate and high lipid diets. Anim. Nutr. https://doi.org/10.1016/j.aninu.2023.01.007
Nopilita, E., Syawal, H., Riauwaty, M., 2016. Histopathology Liver and Kidney of Tilapia (Oreochromis Niloticus) That Were Infected with Bacteria Streptococcus Iniae. J. Online Mhs. Fak. Perikan. dan Ilmu Kelaut. Univ. Riau 3, 1–14.
Pattanayak, S., Priyadarsini, S., Paul, A., Kumar, P.R., Sahoo, P.K., 2020. Diversity of virulence-associated genes in pathogenic Aeromonas hydrophila isolates and their in vivo modulation at varied water temperatures. Microb. Pathog. 147, 104424. https://doi.org/10.1016/j.micpath.2020.104424
Popoola, O.M., Behera, B.K., Kumar, V., 2023. Dietary silver nanoparticles as immunostimulant on rohu (Labeo rohita): Effects on the growth, cellular ultrastructure, immune-gene expression, and survival against Aeromonas hydrophila. Fish Shellfish Immunol. Reports 4, 100080. https://doi.org/10.1016/j.fsirep.2022.100080
Prabu, E., Rajagopalsamy, C.B.T., Ahilan, B., Jeevagan, I.J.M.A., Renuhadevi, M., 2019. Tilapia – An Excellent Candidate Species for World Aquaculture: A Review. Annu. Res. Rev. Biol. 1–14. https://doi.org/10.9734/arrb/2019/v31i330052
Pridgeon, J., Klesius, P., 2011. Molecular identification and virulence of three Aeromonas hydrophila isolates cultured from infected channel catfish during a disease outbreak in west Alabama (USA) in 2009. Dis. Aquat. Organ. 94, 249–253. https://doi.org/10.3354/dao02332
Rahardjo, S., Vauza, M., Rukmono, D., Wiradana, P., 2022. Supplementation of hairy eggplant (Solanum ferox) and bitter ginger (Zingiber zerumbet) extracts as phytobiotic agents on whiteleg shrimp (Litopenaeus vannamei). J. Adv. Vet. Anim. Res. 9, 78. https://doi.org/10.5455/javar.2022.i571
Wiradana, P.A., Sani, M.D., Mawli, R.E., Ashshoffa, F.N.D., Widhiantara, I.G., Mukti, A.T., 2022a. Monitoring the occurrence of Zoea Syndrome (ZS) in pacific white shrimp (Litopenaeus vannamei) larval from several hatcheries in East Java, Indonesia. IOP Conf. Ser. Earth Environ. Sci. 1036, 012003. https://doi.org/10.1088/1755-1315/1036/1/012003
Wiradana, P.A., Yudha, I.K.W., Mukti, A.T., 2022b. Mass tilapia (Oreochromis mossambicus) mortality in floating net cages at Batur Lake, Bangli Regency, Bali Province: a case report. IOP Conf. Ser. Earth Environ. Sci. 1036, 012068. https://doi.org/10.1088/1755-1315/1036/1/012068
Yuan, Yuan, Yuan, Yongming, Dai, Y., Gong, Y., 2017. Economic profitability of tilapia farming in China. Aquac. Int. 25, 1253–1264. https://doi.org/10.1007/s10499-017-0111-8
Zhang, X., Wu, W., Li, L., Ma, X., Chen, J., 2013. Genetic variation and relationships of seven sturgeon species and ten interspecific hybrids. Genet. Sel. Evol. 45, 21. https://doi.org/10.1186/1297-9686-45-21
Zhou, Q., Wang, L., Wang, H., Xie, F., Wang, T., 2012. Effect of dietary vitamin C on the growth performance and innate immunity of juvenile cobia (Rachycentron canadum). Fish Shellfish Immunol. 32, 969–975. https://doi.org/10.1016/j.fsi.2012.01.024
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