COVID-19 MOLECULAR-BASED TESTING CAPACITY IN FIVE CUSTOMARY AREAS OF PAPUA
Background: COVID-19 (Corona Virus Disease-19) is a disease that emerged at the end of December 2019 and was declared a world pandemic by the WHO (World Health Organization) in March 2020. The gold standard for testing for covid-19 until now is molecular-based virus detection with RT-PCR (Real Time-Polymerase Chain Reaction) and TCM (Molecular Rapid Test). In the early of this pandemic, Indonesia, especially Papua, still had difficulty examining COVID-19 due to limited laboratories that could carry out these tests.
Aims: This study aimed to see the laboratory's ability from various regencies representing five customary Papuan areas
Methods: This research was a descriptive study that uses primary data and secondary data collected from the Provincial and Regency Papua COVID-19 Response Acceleration Task Force and the representative hospitals of 5 customary Papuan areas (Saireri, Ha anim, Mee Pago, Lapago, Mamta)
Results: There are several challenges and things that need to be considered by all stakeholders involved in examining COVID-19 in Papua so that the examination capacity can be maximally increased.
Conclusion: Regency that only has GeneXpert are recommended to have qPCR so that the acceleration of COVID-19 testing in Papua Province can be carried out. The qPCR can be procured in several cities/regencies within one customary area so that sample delivery can be done more quickly without having to send samples to Jayapura City
Abdelrazik, A. M., Elshafie, S. M. and Abdelaziz, H. M. (2020) ‘Potential Use of Antigen-Based Rapid Test for SARS-CoV-2 in Respiratory Specimens in Low-Resource Settings in Egypt for Symptomatic Patients and High-Risk Contacts', Laboratory Medicine, pp. 50–53. doi: 10.1093/labmed/lmaa104.
Agustina, A. S. and Fajrunni'mah, R. (2020) ‘Perbandingan Metode RT-PCR dan Tes Rapid Antibodi untuk Deteksi Covid-19', Jurnal Kesehatan Manarang, 6 (Khusus), p. 47. doi: 10.33490/jkm.v6ikhusus.317.
Becker, M. G. et al. (2020) ‘Recommendations for sample pooling on the Cepheid GeneXpert® system using the Cepheid Xpert® Xpress SARS-CoV-2 assay', PLoS ONE, 15(11 November), pp. 1–9. doi: 10.1371/journal.pone.0241959.
Brooke, J. and Jackson, D. (2020) ‘Older people and Covid-19: Isolation, risk and ageism', Journal of Clinical Nursing, 29(13–14), pp. 2044–2046. doi: 10.1111/jocn.15274.
BPD Papua (2020) Profil Singkat Propinsi Papua Berdasarkan Lima Wilayah Adat. Available at: https://penghubung.papua.go.id/5-wilayah-adat/ (Accessed: 21 January 2020).
Chilamakuri, R. and Agarwal, S. (2021) ‘Covid-19: Characteristics and Therapeutics', Cells, 10(2), pp. 1–29. doi: 10.3390/cells10020206.
Corman, V. M. et al. (2020) ‘Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR', Eurosurveillance, 25(3), pp. 1–8. doi: 10.2807/1560-7917.ES.2020.25.3.2000045.
Coronaviruses, V. C. and New, E. (2013) ‘Laboratory biorisk management for laboratories handling human specimens suspected or confirmed to contain novel coronavirus : Interim recommendations Biorisk management checklist for laboratory managers and staff', (February), pp. 1–7.
Djalante, R. et al. (2020) ‘Review and analysis of current responses to Covid-19 in Indonesia: Period of January to March 2020', Progress in Disaster Science, 6, p. 100091. doi: 10.1016/j.pdisas.2020.100091.
Egbuna, O. I. and Brown, E. M. (2013) ‘Genetically Determined Disorders of the Calcium-Sensing Receptor', Genetics of Bone Biology and Skeletal Disease, 19(1), pp. 459–477. doi: 10.1016/B978-0-12-387829-8.00029-9.
Fellahi, S. et al. (2016) ‘Comparison of SYBR green i real-time RT-PCR with conventional agarose gel-based RT-PCR for the diagnosis of infectious bronchitis virus infection in chickens in Morocco', BMC Research Notes, 9(1), pp. 1–9. doi: 10.1186/s13104-016-2037-z.
Geri, P. et al. (2020) ‘Limited role for bronchoalveolar lavage to exclude Covid-19 after negative upper respiratory tract swabs: A multicentre study', European Respiratory Journal, 56(4), pp. 3–4. doi: 10.1183/13993003.01733-2020.
Heid, A. R. et al. (2021) ‘Challenges Experienced by Older People During the Initial Months of the Covid-19 Pandemic', The Gerontologist, 61(1), pp. 48–58. doi: 10.1093/geront/gnaa138.
Januraga, P. P. and Harjana, N. P. A. (2020) ‘Improving Public Access to Covid-19 Pandemic Data in Indonesia for Better Public Health Response', Frontiers in Public Health, 8(November), pp. 8–11. doi: 10.3389/fpubh.2020.563150.
Kaul, K. L. (2020) ‘Laboratories and Pandemic Preparedness: A Framework for Collaboration and Oversight', Journal of Molecular Diagnostics, 22(7), pp. 841–843. doi: 10.1016/j.jmoldx.2020.05.002.
Krishnan, A. et al. (2021) ‘A narrative review of coronavirus disease 2019 (Covid-19): clinical, epidemiological characteristics, and systemic manifestations', Internal and Emergency Medicine, 2019 (0123456789). doi: 10.1007/s11739-020-02616-5.
Leonard, A. et al. (2021) ‘Survey of laboratory medicine's national response to the Covid-19 pandemic in the Republic of Ireland.', Irish journal of medical science, (0123456789). doi: 10.1007/s11845-021-02578-3.
Manual, B. (2020) ‘Laboratory biosafety guidance related to the novel coronavirus ( 2019-nCoV )', Who, (February), pp. 1–12. Available at: https://www.who.int/docs/default-source/coronaviruse/laboratory-biosafety-novel-coronavirus-version-1-1.pdf?sfvrsn=912a9847_2.
Michaud, V. et al. (2020) ‘ACE2 as a Therapeutic Target for Covid-19; Its Role in Infectious Processes and Regulation by Modulators of the RAAS System', Journal of Clinical Medicine, 9(7), p. 2096. doi: 10.3390/jcm9072096.
Mögling, R. et al. (2020) ‘Delayed laboratory response to Covid-19 caused by molecular diagnostic contamination', Emerging Infectious Diseases, 26(8), pp. 1944–1946. doi: 10.3201/eid2608.201843.
Oladimeji, O., Atiba, B. P. and Adeyinka, D. A. (2020) ‘Leveraging polymerase chain reaction technique (GeneXpert) to upscaling testing capacity for SARS-CoV-2 (Covid-19) in Nigeria: a game changer', Pan African Medical Journal, 35(Supp 2), pp. 8–9. doi: 10.11604/pamj.2020.35.2.22693.
Plescia, C. B. et al. (2021) ‘SARS-CoV-2 viral budding and entry can be modeled using BSL-2 level virus-like particles', Journal of Biological Chemistry, 296(8), p. 100103. doi: 10.1074/jbc.ra120.016148.
Pratiwi, Q. (2020) Wagub Papua Minta Percepat Pemeriksaan Swab PCR ODP dan PDP. Available at: https://kabarpapua.co/wagub-papua-minta-percepat-pemeriksaan-swab-pcr-odp-dan-pdp/ (Accessed: 21 January 2020).
Sahli, I. T. et al. (2020) ‘Pola Penyebaran Infeksi Covid-19 di Provinsi Papua Tahun 2020', Gema Kesehatan, 12(1), pp. 30–37. doi: 10.47539/gk.v12i1.133.
Solichin, A. and Khairunnisa, K. (2020) ‘Klasterisasi Persebaran Virus Corona (Covid-19) Di DKI Jakarta Menggunakan Metode K-Means', Fountain of Informatics Journal, 5(2), p. 52. doi: 10.21111/fij.v5i2.4905.
Tahamtan, A. and Ardebili, A. (2020) ‘Real-time RT-PCR in Covid-19 detection: issues affecting the results', Expert Review of Molecular Diagnostics, 20(5), pp. 453–454. doi: 10.1080/14737159.2020.1757437.
Traeger-Synodinos, J. (2006) ‘Real-time PCR for prenatal and preimplantation genetic diagnosis of monogenic diseases', Molecular Aspects of Medicine, 27(2–3), pp. 176–191. doi: 10.1016/j.mam.2005.12.004.
Vlek, A. L. M. et al. (2021) ‘Combined throat/nasal swab sampling for SARS-CoV-2 is equivalent to nasopharyngeal sampling', European Journal of Clinical Microbiology and Infectious Diseases, 40(1), pp. 193–195. doi: 10.1007/s10096-020-03972-y.
Wahjudi, M. (2020) ‘Kontroversi Metode Deteksi Covid-19 di Indonesia', KELUWIH: Jurnal Kesehatan dan Kedokteran, 2(1), pp. 32–42. doi: 10.24123/kesdok.v2i1.2994.
Wu, Z. and McGoogan, J. M. (2020) ‘Characteristics of and Important Lessons from the Coronavirus Disease 2019 (Covid-19) Outbreak in China: Summary of a Report of 72314 Cases from the Chinese Center for Disease Control and Prevention', JAMA - Journal of the American Medical Association, 323(13), pp. 1239–1242. doi: 10.1001/jama.2020.2648.
Yusra, Y. and Pangestu, N. (2020) ‘Pemeriksaan Laboratorium pada Coronavirus Disease 2019 (Covid-19)', Medica Hospitalia : Journal of Clinical Medicine, 7(1A), pp. 304–319. doi: 10.36408/mhjcm.v7i1a.472.
Zhang, F. et al. (2020) ‘A protocol for detection of Covid-19 using CRISPR diagnostics', Bioarchive, pp. 1–8.
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