EFEK PEMBERIAN EKSTRAK RUMPUT KEBAR (Biophytum petersianum Klotzsch) TERHADAP JUMLAH SEL LEYDIG MENCIT (Mus musculus) JANTAN YANG DIPAPAR 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN
This study was aim to examine the effect of kebar grass extract (Biophytum petersianum Klotzsch) to against number leydig cells of mice (Mus musculus) by exposed 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD). Twenty five male mice (Mus musculus) 4 months with avarage body weight 20 g were used. These animals were divided into five groups (K(-), K(+), P1, P2 and P3). K(-) was treated with placebo, K(+) was treated by exposed TCCD single dose injection intaperitoneal 7µg/KgBw, P1 was treated by exposed TCCD single dose injection intaperitoneal 7µg/KgBw and kebar grass extract 0,045 mg/g Bw/day P2 was treated by exposed TCCD single dose injection intaperitoneal 7µg/KgBw and kebar grass extract 0,080 mg/g Bw/day, P3 was treated by exposed TCCD single dose injection intaperitoneal 7µg/KgBw and kebar grass extract 1,350 mg/g Bw/day. This research has been conducted for 53 days. The data were compared using ANOVA and Duncan test by SPSS 22.4 for windows. The result showed that Kebar Grass Extract in all of groups can prevent the damage of leydig cells in testis that exposed by TCCD significantly (p<0,05) and kebar grass extract 0,135 mg/kgBw/day can increase amount of leydig cells maximaly.
Chen, B.B., B.R. Zirkin and R.S. Ge. 2010. The Leydig Cell as a Target for Toxicants. Elsevier. USA.133-142
Dhanabalan, S., PP. Mathur and P. Latha. 2015. TCDD and Corticosterone On Testicular Steroidogenesis and Antioxidant System of Epididymal Sperm in Rats. Toxicology and Industrial Health. 31(9): 811-822
Lai, K.P., M.H. Wong and C.K.C. Wong. 2005. Inhibition of CYP450scc Expression In Dioxin-Exposed Rat Leydig Cells. Journal Of Edocrinology. 185: 519-527.
Latchoumycandane, C., K.C. Chitra, and P.P. Mathur. 2003. 2,3,7,8-Tetrachlorodibenzo-p-Dioxin (TCDD) Induces Oxidative Stress in The Epididymis and Epididymal Sperm of Adult Rats. Arch Toxicol. 77: 280-284.
Sayuti, Kesuma dan Yenrina, R. 2015. Antioksidan Alami dan Sintetik. Padang. Andalas University Press.
Sembiring B dan I Darwati. 2014. Rumput Kebar (Biophytum petersianum) sebagai Peningkat Fertilitas. Warta Puslitbangbun. 19(2): 15-18.
Unitly, A.J.A. dan C. Inara. 2011. Potensi Rumput Kebar (Biophytum petersianum Klotzsch) dalam Meningkatkan Kinerja Reproduksi. Pascasarjana Institute Pertanian Bogor. Prosiding Seminar Nasional. Bogor.
Yoshida, R. and Y. Ogawa. 1999. Review Articel Oxiative Stress Induced by 2,3,7,8-Tetrachlorodibenzo-p-Dioxin: an Application of Oxidative Stress Markers to Cancer Risk Assessment of Dioxins. National Institute of Industrial Health, 21-1.
Journal of Basic Medical Veterinary (JBMV) by Unair is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
1. The journal allows the author to hold the copyright of the article without restrictions.
2. The journal allows the author(s) to retain publishing rights without restrictions
3. The legal formal aspect of journal publication accessibility refers to Creative Commons Attribution Share-Alike (CC BY-SA).
4. The Creative Commons Attribution Share-Alike (CC BY-SA) license allows re-distribution and re-use of a licensed work on the conditions that the creator is appropriately credited and that any derivative work is made available under “the same, similar or a compatible license”. Other than the conditions mentioned above, the editorial board is not responsible for copyright violation.