Antibacterial Activity of Bidara Leaf Extract (Ziziphus mauritiana) Against Staphylococcus aureus Isolated from Mastitis Case In Vitro

Staphylococcus aureus mastitis bidara leaf antibacterial

Authors

  • Afif Tasya Saraswati Student, Faculty of Veterinary Medicine, Universitas Airlangga
  • Rahmi Sugihartuti Division of Basic Veterinary Medicine, Faculty of Veterinary Medicine, Universitas Airlangga
  • Yulianna Puspitasari
    yulianna-puspitasari@fkh.unair.ac.id
    Division of Veterinary Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga
  • Adiana Mutamsari Witaningrum Division of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Airlangga
  • Kadek Rachmawati Division of Basic Veterinary Medicine, Faculty of Veterinary Medicine, Universitas Airlangga
  • Lilik Maslachah Division of Basic Veterinary Medicine, Faculty of Veterinary Medicine, Universitas Airlangga
  • Hartanto Mulyo Raharjo Division of Veterinary Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga
  • Mirza Atikah Madarina Hisyam Division of Veterinary Clinic, Faculty of Veterinary Medicine, Universitas Airlangga
  • Martia Rani Tacharina Division of Veterinary Microbiology, Faculty of Veterinary Medicine, Universitas Airlangga
December 1, 2023

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This research aimed to know the antibacterial activities of bidara leaf extract against Staphylococcus aureus isolated from mastitis cases. The isolate was identified before the antibacterial test. This study was done by five times repetitions on Mueller Hinton Agar. The concentrations used in this study were 30%, 40%, and 50% of bidara leaf extract then a blank disc was dipped into each concentration. Tetracycline disc was used as a positive control and aquadest was used as a negative control. The diameter of the clear zone was measured using calipers after incubation for 24 hours at 37℃. The results showed significant differences (p<0.05) between the positive control (K+), negative control K (-), and the concentration group. However, each concentration of bidara leaf extract showed insignificant differences in the diameter of the clear zone. It could be concluded that bidara leaf extract has antibacterial activities against Staphylococcus aureus isolated from mastitis cases.

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