Effectiveness of Bay Leaf Extract (Syzygium polyanthum) on Uric Acid and Cholesterol Levels in Caffeine-Induced Male Mice (Mus musculus)
Downloads
Bay leaf (Syzygium polyanthum) is one of the plants from Indonesia that has the potential to be used as a raw material for herbal medicine. Pharmacologically, bay leaves containing flavonoids have been shown to have antioxidant activity, which can reduce hyperuricemia and blood cholesterol levels. This study aims to determine the effectiveness of bay leaf extract in reducing uric acid and cholesterol levels in the blood of mice induced by caffeine. The mice tested animals were divided into six groups, namely a positive control group of uric acid, a positive control of cholesterol, where a positive control of uric acid was given allopurinol and a positive control of cholesterol was given simvastatin, a negative control was given Na-CMC 1%, and a group that was given bay leaf extract in different dose levels, namely P1 was given 20 mg/kg BB, P2 was given 40 mg/kg BB, and P3 was given 80 mg/kg BB. The parameters used were the decrease in blood uric acid and cholesterol levels caused after the treatment was measured using a POCT strip test for uric acid and cholesterol. The data obtained were statistically processed using the One-Way Anova test. The results of the Anova test showed a significant difference (p<0.05) in the data on uric acid and cholesterol levels. Then continued with the results of Duncan's test to see the most effective dose level for bay leaf extract in reducing uric acid and cholesterol levels. In Duncan's test results, it was found that the P3 group of mice with a dose of 80 mg/kg BB was the most effective dose level in reducing uric acid and cholesterol levels in the blood of mice induced by caffeine.
Ana Fitri, 2007. “Pengaruh Penambahan Daun Salam (Eugenia Polyantha Wight) Terhadap Kualitas Mikrobiologis, Kualitas Organoleptis dan Daya Simpan Telur Asin pada Suhu Kamar”, Skripsi (Surakarta : Program studi strata satu Universitas Sebelas Maret, 2007), hlm. 13.
Andri, W.Y. 2007. Produksi Mencit Putih (Mus Musculus) dengan Substitusi Bawang Putih (Allium Sativum) dalam Ransum, Skripsi. Fakultas Peternakan Institut Pertanian Bogor. P. 3-5.
Ariyanti, R., Wahyuningtyas, N., dan Wahyuni, A.S., 2007, Pengaruh Pemberian Infusa Daun Salam (Eugenia Polyantha Wight ) terhadap Penurunan Kadar Asam Urat Dalam Darah Mencit Putih Jantan yang Diinduksi Potasium Oksonat, Pharmacon, 8 (2),56-63.
Artini NPR, Wahyuni. 2012. Ekstrak Daun Sirsak (Annona Muricata L. terhadap penurunan Kadar Asam Urat Darah Tikus Wistar. Jurnal Kimia Fakultas MIPA Universitas Udayana. Vol 6: 121-137.
Azizahwati, Wiryowidagdo, S., dan Prihandini, K., 2005. Efek penurunan kadar asam urat dalam darah pada tikus jantan dari rebusan akar tanaman akar kucing (Acalypha indica Linn), Jurnal Bahan Alam Indonesia, 4(1):213-218.
Backer CA. Flora of java (spermatophytes only). Noordhoff-Groningen: Bakhuizen van den Brink RC Jr; 1963.
Badan POM RI., 2004, Monografi Ekstrak Tumbuhan Obat Indonesia.
Budiharto, 2008. Metodologi Penelitian Kesehatan dengan Contoh Bidang Ilmu Kesehatan Gigi. Jakarta: Penerbit IKAPL. hlm. 51.
Depkes, RI., 2000. Parameter Standar Umum Ekstrak Tumbuhan Obat. Direktorat Jendral Pengawasan Obat dan Makanan, Direktorat Pengawasan Obat Tradisional. Jakarta.
Departemen Kesehatan Republik Indonesia. 2008. Farmakope Herbal Indonesia. Jakarta.
English J, 2004.New dietary supplement shows dramatic effects in lowering cholesterol, ldl, trigliserydes. 4 Januari 2011.
Falconer, D.S. 1981. Introduction to Quantitative Genetics. Longman, London and New York.
Kelley WN, Wortmann RL. Gout and hyperuricemia. Dalam: Kelley WN, editor.Textbook of rheumatology. Edisi 5. Philadelphia: WBSaunders Company; 1997. hlm.1314-50.
Malik, A., dan Ahmad, A.R. 2013. Antidiarrheal Activity of Etanolic Extract of Bay Leaves (Syzygium Polyanthum Wight). Int. Res. J. Pharm. 4(4) : 106-108.
Nety Wulandari,“Pengaruh Pemberian Ekstrak Syzygium polyanthum Terhadap Produksi ROI Makrofog Pada Mencit BALB/c yang Diinokulai Salmonella typhimurium”, Skripsi (Semarang : Universitas Diponegoro, 2006), hlm.19.
Nijveldt, R. J., Nood, E. V., Hoorn, D. E. V., Boelens, P. G., Norren, K. V., dan Leeuwen, P. A. V. 2001. Flavonoids: A Review of Probable Mechanisms of Action and Potential Applications. American Journal of Clinical Nutrition 74(4): 418-425.
Price & Wilson. 2005. Patofisiologi : Gangguan Sistem Kardiovaskular. Jakarta : EGC pp. 582-283.
Putra TR. Hiperurisemia. Dalam: Sudoyo AW, editor. Buku ajar penyakit dalam.Edisi 4. Jakarta:Pusat Penerbitan Departemen Ilmu Penyakit Dalam Fakultas Kedokteran Universitas Indonesia; 2006. hlm. 1213-7.
Rizqiana Dewi, Aktivitas Antioksidan dan Sitotoksisitas Metabolit Sekunder Daun Salam (Syzygium polyanthum Wight) Dan Daun Jati Belanda (Guazuma ulmifolia Lamk.), Skripsi, (Bogor : Program studi strata satu Institut Pertanian Bogor, 2012), hlm. 3.
Rustika. 2002. Pola konsumsi minyak goreng keluarga dan peningkatan risiko kolesterol. http://digilib.litbang.depkes.go.id. 16 Desember 2010.
Saputra R. Efek fraksi etil asetat ekstrak etanol daun salam (Syzygium polyanthum Wight.) terhadap penurunan kadar asam urat darah mencit putih jantan galur balbc yang diinduksi kalium oksonat [skripsi]. Surakarta: Universitas Muhammadiyah Surakarta; 2008.
Silbernagl, S. 2006. In: Silbernagl, S., Lang, F. editor. Teks dan Atlas Berwarna Patofisiologi. Jakarta : EGC.
Sinaga AF, Widdhi B, Widya AL. Uji efek etanol daun salam terhadap penurunan kadar asam urat tikus putih jantan galur wistar yang diinduksi potassium oksalat. Manado: Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Sam Ratulangi; 2014.
Smith John B dan Mangkoewidjojo Soesanto. 1988. Pemeliharaan, Pembiakan, dan Penggunaan Hewan Percobaan di Daerah Tropis Jakarta. Universitas Indonesia Salemba 4. Hlm. 37 -48.
Soedarsono, et al. Tumbuhan obat pusat. Jillid 2. Yogyakarta: Studi Obat Tradisional; 2002. hlm. 174.
Sudarmi. 1997. Kafein dalam Pandangan Farmasi. Medan: Fakultas Matematika dan Ilmu Pengetahuan Alam (FMIPA) Universitas Sumatera Utara (USU).
Sutanto T. Asam Urat deteksi, pencegahan, pengobatan. Yogyakarta: Penerbit BukuPintar; 2003.
Utami IW. Efek fraksi air ekstrak etanol daun salam (Syzygium polyanthum Wight.) terhadap penurunan kadar asam urat pada mencit putih (Mus musculus) jantan galur balb-c yang diinduksi dengan kaliumoksinat [skripsi].Surakarta: Universitas Muhammadiyah Surakarta; 2008.
Wachjudi G, et al. Diagnosis dan terapi penyakit reumatik. Jakarta:Sagung Seto; 2006.
Wade A, Waller PJ. Handbook of pharmaceutical excipient second sedition. London The Pharmaceutical Press. 1994.
Waspadji S, dkk 2003. Indeks Glikemik Berbagai Makanan Indonesia. Jakarta : Fakultas Kedokteran Universitas Indonesia.
Wartini, N.M. 2009. Senyawa Penyusun Ekstrak Flavor Daun Salam (Eugenia polyantha Wight) Hasil Distilasi Uap Menggunakan Pelarut n-Heksana dan Tanpa n-Heksana. Agrotekno 15(2): 72- 77.
Wortman RL. Disorders of purine and pyrimidine metabolism. Dalam: Kasper DL, editor.Harrison’s principles ofinternal medicine. Edisi 16. Volume 2. New York McGraw-Hill; 2005. hlm. 2308-13.
Copyright (c) 2024 Fahrul Andika Pratama, Salma Aisyafalah, Rochmah Kurnijasanti
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Journal of Basic Medical Veterinary (JBMV) by Unair is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
1. The journal allows the author to hold the copyright of the article without restrictions.
2. The journal allows the author(s) to retain publishing rights without restrictions
3. The legal formal aspect of journal publication accessibility refers to Creative Commons Attribution Share-Alike (CC BY-SA).
4. The Creative Commons Attribution Share-Alike (CC BY-SA) license allows re-distribution and re-use of a licensed work on the conditions that the creator is appropriately credited and that any derivative work is made available under "the same, similar or a compatible license”. Other than the conditions mentioned above, the editorial board is not responsible for copyright violation.
Perhimpunan Dokter Hewan Indonesia
