Immunohistochemical Detection of Coxiella burnetii in Cattle Spleen Organs from Ampel Slaughterhouse, Boyolali Regency

Coxiella burnetii immunohistochemical cattle Boyolali

Authors

  • Eko Prasetyo Nugroho
    drh.koko@gmail.com
    Sekolah Pascasarjana IPB, Program Studi Ilmu Biomedis Hewan, Fakultas Kedokteran Hewan, Institut Pertanian Bogor
  • Agus Setiyono Laboratorium Patologi, Divisi Patologi Veteriner IPB, Fakultas Kedokteran Hewan, Institut Pertanian Bogor
  • Upik Kesumawati Hadi Laboratorium Entomologi, Divisi Parasitologi dan Entomologi Veteriner IPB, Fakultas Kedokteran Hewan, Institut Pertanian Bogor
  • Wiwin Winarsih Laboratorium Patologi, Divisi Patologi Veteriner IPB, Fakultas Kedokteran Hewan, Institut Pertanian Bogor
  • Dwi Astuti Pusat Penelitian Biologi, Divisi Zoologi, Lembaga Ilmu Pengetahuan Indonesia Bogor
April 15, 2021

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Q-fever is a zoonotic bacterial disease that caused by Coxiella burnetii. These microorganism are gram negative and obligate intracellular bacteria. This study was conducted to detect C. burnetii in cattle organs which collected from Ampel slaughterhouse Boyolali Regency. In this study, spleen, heart, lung, liver and kidney were collected from 100 cattle. The samples were tested by immunohistochemical (IHC) method using polyclonal anti- C. burnetii antibodies. Immunohistochemical examination found the presence of C. burnetii in the cytoplasm of macrophage cells with specific brown color only in the spleen as many as 4 out of 100 cattle showing immunoreactive (4%). The four positive individual samples were from Simental cattle. These results indicate that Q-fever was found in local cattle in Boyolali Regency.

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