Stres Dapat Mengganggu Proses Spermatogenesis pada Mencit
Downloads
Method: This was an experimental study using a completely randomized design. The variables examined were the number of spermatogenic cells (spermatogonia, spermatocytes, and spermatids oval) and testoseron levels. Data analysis using oneway analysis of variance (ANOVA), test a small real difference (LSD) 5%, and the kruskal wallis test. This study used 36 mice (Mus musculus) male age 2 months with 20–40 gram body weight divided into 4 groups. The control group (subcutaneous injection of 0.1 ml physiological saline), subcutaneous epinephrine injection group 0.001 mg/20 g BW, subcutaneous epinephrine injection group 0.005 mg/20 g BW, the subcutaneous injection of epinephrine 0.01 mg/20 BB gr.
Result: The results show that the analysis of spermatogenic cells (spermatogonia, spermatocytes, and spermatids oval), that repeated exposure to epinephrine with different doses showed that there was a significant decrease. The average decline in a row in the treated group (P1, P2, P3) compared with the control group (K0) for spermatogonia was 28.8%, 42.4%, 45.5%, to spermatocytes was 13.7%, 37.4%, 38.9% and for oval spermatids was 33.3%, 44.2%, 50.4% Having followed by LSD 5% found that there were significant differences for almost all groups except the pair P2–P3 groups for the number of spermatogonia, spermatocytes, and partner groups P1–P2 and P2–P3. As for the blood serum levels of testosterone hormone mice showed no significant decrease.
Discussion: Administration of high doses of epinephrine (as stressor) can lead to bottlenecks in the process of spermatogenesis as indicated by decreased number of spermatogenic cells but not cause a decrease in testosterone levels.
Antony, 1995. Endocrinology of the Male Reproductive Sistem, (Online), (http// www.endotext.org/male/male2/male1.
htm., diakses tanggal 5 agustus 2006).
Arief, M., Triyanti Kuspuji, Savitri Rakhmi, Wardhani Wahyu Ika, Setiowulan Luiwik, 1999. Kapita Selekta Kedokteran. Jakarta: Media Aesculapius.
Cunningham, G., 2002. Textbook of Veterinay Physiology, 3rd edition. WB. Souinders Company. Philadhelpia pp: 356–357.
Ganong, W.F., 2001. Review of Medical Physiology, 20th ed. Appleton and Large, Stanford, Connectut, pp 545–567.
Guyton, A.C., 2000. Texbook of Medical Physiology, 10th ed. West Washington Aquare: WB Saunder Company, pp: 1284–1298.
Ismudiono, 1999. Fisiologi reproduksi pada ternak. Edisi 2, Surabaya: Fakultas Kedokteran Hewan Universitas Airlangga, hlm 70–79.
Johnson, H., M., J. Everitt, 2000. Essential Reproduction. Fifth edition, Blackwell Science Ltd, 53–68, 102–103.
Kusumawati, D., 2003. Bahan Ajar tentang HEWAN COBA. Surabaya: Fakultas Kedokteran Hewan Universitas Airlangga, hlm. 11–2, 22, 67, 87.
Prato, F.S., Choleris, E., Thomas, A.W., Moran, G.R., 2001. Behavioural stress responses of mice may be sensitive to weak, ambient elf agnetic fi elds on the order of 0.1 uT. Bioelectromagnetics Society, 23rd Annual Meeting, 11–14 June, St. Paul, MN,
Sugiyono, Wibowo Eri, 2001. Statistik Penelitian dan Aplikasinya dengan SPSS 10.0 for windows, Bandung: Alfabeta.
Authors who publish with Jurnal Ners agree to the following terms:
- Authors transfer the Copyright and grant Jurnal Ners the right of first publication with the work simultaneously licensed under a Creative Commons Attribution 4.0 International License that allows others to remix, adapt and build upon the work with an acknowledgment of the work's authorship and of the initial publication in Jurnal Ners.
- Authors are permitted to copy and redistribute the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in Jurnal Ners.
Jurnal Ners requires a formal written declaration and transfer of copyright from the author(s) for each article published. We, therefore, ask you to complete and return this form, retaining a copy for your own records. Your cooperation is essential and appreciated. Any delay will result in a delay in publication. The form can be downloaded HERE.
