Brain Derived Neurotrophic Factor Levels in Aged Rats Post-Systemic Human Mesenchymal Stem Cell Administration

adisti dwijayanti

Abstract views = 509 times | downloads = 320 times


Brain-Derived Neurotrophic Factor (BDNF) levels were affected by aging. Brain BDNF levels were known to decrease along with advanced age thus correlated with any diseases such as cognitive impairment and Alzheimer. Mesenchymal Stem Cell (MSC) is one of the potential modalities actively investigated against age-related diseases. This study evaluated the effect of human MSC administration to brain BDNF levels in aged rats. Intravenous injection of 10 million per body weight human MSC were given four times in 3 months interval to 22-24 months old female and male Spraque–Dawley rats. As control group, aged rats were injected by normal saline at the same volume and frequencies. Moreover, young 3-6 months rats also examined as negative control.  By the end of the experiment, we analyzed three rats from each group. Brain BDNF levels were measured by enzyme-linked immunosorbent assay and normalize to the protein levels. One-way ANOVA and LSD post hoc analysis was performed to compare the differences between groups. BDNF levels in male appeared similar between young, aged, and MSC treated groups. Meanwhile, control aged female groups had significantly lower BDNF levels compared to young (p = 0.019) and MSC-treated aged rats (p = 0.001). There was no difference of BDNF levels between young and MSC-treated aged in female rats (p = 0,068). Both sex had similar BDNF levels (p = 0.249) in control-aged groups. In contrast, female young and MSC-treated aged rats achieved significantly higher BDNF levels (p = 0.009 and p <0.001) compared to the male groups, respectively. These results suggest that human mesenchymal stem cell intravenous injection can increase brain BDNF levels in female aged rats.


BDNF, human mesenchymal stem cell, aged rats.

Full Text:



Bettio LEB, Rajendran L, Gil-Mohapel J. 2017. The effects of aging in the hippocampus and cognitive decline. J Neu Bio Rev. 79:66–86.

Budni J, Bellettini-Santos T, Mina F, Garcez ML, Zugno AI. 2015. The involvement of BDNF, NGF and GDNF in aging and alzheimer's disease. Aging Dis. 6(5): 331–41.

Chan CB and Ye K. 2017. Sex differences in brain-derived neurotrophic factor signaling and functions. J Neurosci Res. 95(1-2): 328–35.

Coria-lucero CD, et al. 2016. Rhythmic bdnf and trkb expression patterns in the prefrontal cortex are lost in aged rats. Brain Res. 1653: 51–8.

De Bruyn C, et al. 2011. A rapid, simple, and reproducible method for the isolation of mesenchymal stromal cells from Wharton's jelly without enzymatic treatment. Stem Cells Dev. 20(3): 547–57.

Kim D, et al. 2015. Health span-extending activity of human amniotic membrane- and adipose tissue-derived stem cells in F344 rats. Stem Cells Transl Med. 4(10): 1144–54.

Komulainen P, et al. 2008. BDNF is a novel marker of cognitive function in ageing women: the DR’s EXTRA study. Neurobiol Learn Mem. 90: 596–603.

Murinova J, Hlavacova N, Chmelova M, Riecansky I. 2017. The evidence for altered BDNF expression in the brain of rats reared or housed in social isolation: a systematic review. Front Behav Neurosci. 11: 101.

Park D, et al. 2013. Human adipose tissue-derived mesenchymal stem cells improve cognitive function and physical activity in ageing mice. J Neurosci Res. 91: 660–70.


  • There are currently no refbacks.

Copyright (c) 2019 Journal of Stem Cell Research and Tissue Engineering

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

View Stats

Creative Commons License
JSCRTE by Unair is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.