Stem Cell from Human Exfoliated Deciduous Teeth (SHED) versus Human Umbilical Cord Blood Mononuclear Cells (cbMNC) Transplantation in Neural Damage Reduction in Rat Model of Cerebral Ischemia

yetty ramli

Abstract views = 631 times | downloads = 443 times


Ischemic stroke is one of major cause of mortality and disability in Indonesia. Stem Cells are considered as a promising therapy for ischemic stroke. In this study, we compared therapeutic potency of Stem cell from human exfoliated deciduous teeth (SHED) and Human umbilical cord blood mononuclear cell (cbMNC) using rat models of ischemic stroke. Following middle cerebral artery occlusion (MCAO), twenty male wistar rats were divided into four groups : normal rats (n=5), rats undergone permanent MCAO (n=5) as the control (stroke) group, rats undergone permanent MCAO and SHED transplantation (n=5) and rats undergone permanent MCAO and cbMNC transplantation (n=5) as the treatment group. SHED transplantation was performed at the acute phase after MCAO by intravenous injection. Histopathological evaluation of the neuron death ratio with hematoxylin and eosin staining confirmed that there was no significant differences at comparative study of neuron death ratio in rats transplanted with SHED and rats transplanted with cbMNC (p=0,81). SHED and cbMNC transplantation at acute stroke showed reduction in the neuron death ratio in the brain of rat models with ischemic stroke, and may provide an opportunity for neuroprotection and neural regeneration after ischemic stroke.


cbMNC, Cell transplantation, Ischemic stroke, MCAO, SHED

Full Text:



Badan Penelitian dan Pengembangan Kesehatan. Riset Kesehatan Dasar (RISKESDAS) 2013 Lap Nas 2013. 1–384.

Boltze J, Schmidt UR, Reich DM, Kranz A, Reymann KG, Strassburger M, et al 2012 Determination of the therapeutic time window for human umbilical cord blood mononuclear cell transplantation following experimental stroke in rats. Cell Transplant 21 1199–211

Chen J, Li Y, Katakowski M, Chen X, Wang L, Lu D, et al 2003 Intravenous bone marrow stromal cell therapy reduces apoptosis and promotes endogenous cell proliferation after stroke in female rat. J Neurosci Res 73 778 – 86.

Chen J, Zhang ZG, Li Y, Wang L, Xu YX, Gautam SC, et al 2003 Intravenous administration of human bone marrow stromal cells induces angiogenesis in the ischemic boundary zone after stroke in rats Circ Res 92 : 692 – 9.

Fruchtman SM, Hurlet A, Dracker R, Isola L, Goldman B, Schneider BL, Emre S 2004 The successful treatment of severe aplastic anemia with autologous cord blood transplantation. Biol Blood Marrow Transplant 10 741-2

Ikegame Y,et al 2011 Comparison of mesenchymal stem cells from adipose tissue and bone marrow for ischemic stroke therapy Cytotherapy 13 675–85.

Inoue T, Sugiyama M, Hattori H, Wakita H, Wakabayashi T, Ueda M 2013 Stem Cells from Human Exfoliated Deciduous Tooth-Derived Conditioned Medium Enhance Recovery of Focal Cerebral Ischemia in Rats Tissue Eng Part A 19 1–2.

Iskander A, Knight RA, Zhang ZG, Ewing JR, Shankar A, Varma NR, et al 2013 Intravenous administration of human umbilical cord blood-derived AC133+ endothelial progenitor cells in rat stroke model reduces infarct volume:magnetic resonance imaging and histological findings. Stem Cells Transl Med 2 703–14.

Johnson W, Onuma O, Owolabi M, Sachdev S 2016 Stroke: A global response is needed. Bulletin of the World Health Organization.

Mita T, Furukawa-Hibi Y, Takeuchi H, Hattori H, Yamada K, Hibi H, et al 2015 Conditioned medium from the stem cells of human dental pulp improves cognitive function in a mouse model of Alzheimer’s disease. Behav Brain Res.

Lei, et al 2017 Intraarterial transplantation of human umbilical cord blood mononuclear cells in hyperacute stroke improves vascular function. Stem Cell Research & Therapy 8:74

Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, et al 1999 Multilineage potential of adult human mesenchymal stem cells Science 284 143 – 7

Ramli Y, Alwahdy AS, Kurniawan M, Juliandi B, Wuyung PE, Bilianti Susanto YD 2017 Intravenous Versus Intraarterial Transplantation of Human Umbilical Cord Blood Mononuclear Cells for Brain Ischemia in Rats HAYATI J Biosci 24 187–94

Ringden O, Okas M, Uhlin M, Uzunel M, Remberger M, Mattsson J 2008 Unrelated cord blood and mismatched unrelated volunteer donor transplants, two alternatives in patients who lack an HLA-identical donor. Bone Marrow Transplant 42 643-8

Rosa V, Dubey N, Islam I, Min KS, Nor JE 2016 Pluripotency of Stem Cells from Human Exfoliated Deciduous Teeth for Tissue Engineering. Stem Cells International 1–6.

Wei X, Xue Y, Zhi-peng H, Fan-fang Q, Li S, Yu-fang S 2013 Mesenchymal stem cells: a new trend for cell therapy. Acta Phamacol Sin 34 747-54.

Yamagata M, Yamamoto A, Kako E, Kaneko N, Matsubara K, Sakai K, et al 2013 Human dental pulp-derived stem cells protect against hypoxic-ischemic brain injury in neonatal mice Stroke 44 551–4.


  • There are currently no refbacks.

Copyright (c) 2019 Journal of Stem Cell Research and Tissue Engineering

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

View Stats

Creative Commons License
JSCRTE by Unair is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.